BIORESOURCE TECHNOLOGY
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2025
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IF 9
Xin Jin,Sumeng Wang,Yaping Gao,Qingsheng Qi,Quanfeng Liang
Background The industrial production of L-threonine faces challenges because of high production costs, especially those of substrates, meaning new production methods are needed. Methods Fur, a new global transcription factor related to L-threonine biosynthesis, was discovered in this study. Multidimensional regulation combined with global transcriptional machinery engineering was used to modify an Escherichia coli strain. Results: The most efficient mutant showed high titer (154.2 g/L), productivity (2.14 g/L/h), and yield (0.76 g/g) of L-threonine production. These three parameters indicated that these engineering strategies were economically feasible for developing high L-threonine-producing strains. We integrated the sucrose utilization gene cluster into the genome to further reduce the production cost of L-threonine. Using untreated cane molasses as the substrate, L-threonine was successfully produced with a titer of 92.46 g/L and a cost reduction of 48 %. Conclusion This research offers advantages for industrial scalability, and the resulting engineered bacterium holds significant industrial application potential.
研究领域:
生物工程
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
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2025
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IF 2.4
Tian Jingyin,Zhao Guoyu,Zhou Xia,Hu Feng,Shao Kangmeng,Dassanayaka Sajith Madhusanka,Jin Linhong
The successful commercialization of entomopathogenic nematodes (EPNs) as a biopesticide product depends on industrial mass production of viable infective juveniles (IJs) at low cost. This study focused on enhancing the yield of the Steinernema carpocapsae All (Weiser) and reducing its production costs. An optimal basal medium was screened among several basal media, and several optimized components were included in the selected optimal basal medium. The optimal medium formulation was obtained by evaluating the yield, virulence, and survival rates of S. carpocapsae All at different times. The results showed that when isopropylstilbene was added to the basal medium together with insulin, the yield increased from 13.14 × 104 IJs/ml with the basal medium to 19.63 × 104 IJs/ml. At 30 d of low temperature storage, the survival rate increased from 31.42% (basal medium) to 56.83% (optimal medium), demonstrating a prolonged the shelf life. The virulence of S. carpocapsae All was measured before and after 30 d of storage on sponges. The virulence against Tenebrio molitor (Linnaeus) (Coleoptera: Tenebrionidae) and Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) was 90 and 86.67% (before) and 93.33 and 94.44% (after), respectively, when insects were treated at 48 h, long-term cryogenic storage has no effect on the virulence. The yield with the individual optimization components added to the optimal basal medium was lower than when the components were combined, indicating that there may be a synergy between the 2 components. The results would provide practical technology for low-cost commercial production of S. carpocapsae All and promote field applications.
研究领域:
农业昆虫学
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生物防治
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线虫学
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微生物发酵
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应用昆虫学
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产品关联
DOI
PubMed
高影响因子
Nature Sustainability
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2024
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IF 25.7
Chen Chaohuang,Zhou Qianhai,Guo Zhongyuan,Li Hao,Miao Chen,Chen Du,Hu Xiaohong,Feng Xia,Noël Vincent,Ghoshal Subhasis,Lowry Gregory V.,Zhu Lizhong,Lin Daohui,Xu Jiang
Using nanoscale zero-valent iron (nFe 0 ) materials for heavy metal removal is a viable approach for in situ groundwater pollution remediation. However, conventional nFe 0 materials have indiscriminate reactivity towards various electron acceptors (for example, water) and just accumulate heavy metals onto the surface, which leads to poor selectivity and short longevity. Here we develop a lattice-sulfur-impregnated nFe 0 (S-nFe 0 ), achieving intraparticle sequestration of heavy metals enabled by a boosted Kirkendall-like effect. This metal-encapsulation approach outcompetes water for electrons and efficiently uses Fe-released spots, and the reacted S-nFe 0 becomes inert to release metals (78–220× less than nFe 0 ) in real groundwater matrices. The treated groundwater is estimated to meet drinking-water standards with a longevity of over 20–100 years. The synthesis of S-nFe 0 has negligible environmental impacts according to Biwer–Heinzle environmental evaluation results. S-nFe 0 also shows competitive production and operation costs for metal-contaminated groundwater remediation. Overall this work presents a strategy for achieving metal encapsulation in nFe 0 , which breaks the reactivity–selectivity–stability trade-offs of redox nanomaterials, providing a powerful tool to tackle groundwater pollution.
研究领域:
环境科学
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材料科学
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化学工程
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水资源管理
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Liangyuan Qi,Wei Cai,Tianyang Cui,Bichen Lin,Zefan Feng,Jing Gao,Guangyong Jiang,Wenyue Guo,Chuanshen Wang,Bin Fei,Jixin Zhu,Yuan Hu,Weiyi Xing,Lei Song
Even though the huge potential in significantly decreasing the energy consumption for cooling buildings and the human body is presented by radiative cooling technology, there is still a long way to realize its real-commercial application, considering comprehensive performances, such as safety, comfort, longevity, and production cost. Inspired by the biological micro-nano structures of Cyphochilus, we develop a one-step, large-scale, and low commercial-cost method to prepare multifunctional composite textiles, with effective cooling, fire safety, and directional water transportation. The high emissivity (96.8 %) in the atmospheric window and high reflectivity (97.1 %) in the solar waveband are successfully achieved. When exposed to direct sunlight, composite textiles can achieve up to 5 °C below commercial textiles at a solar intensity of 750 W•m −2 . Besides, the directional water transportation and the excellent mechanical strength further improve the practical application. This work proposes a radiatively cooled composite textile to achieve personal thermal management and fire protection to meet the needs of specialty garments such as firefighting suits.
研究领域:
材料科学
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纺织工程
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热能工程
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生物启发
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可持续技术
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
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IF 8.355
Jie Ma,Lei Wang,Fei Yu,Xiaohu Dai
Due to its low energy consumption, capacitive deionization (CDI) is regarded as one of the most promising technologies in brackish water desalination. However, CDI has two drawbacks, namely, low deionization capacity and unavoidable parasitic reactions that limit its further development. Unlike the traditional CDI electrodes that store the ions in the electric double layer capacitors, faradic electrodes store ions at crystallographic sites or between the atomic planes, which could effectively address the issues mentioned above. However, obtaining low-cost faradic electrode materials with a high salt removal capacity for practical applications is still challenging. Here, we report a new material, mesoporous amorphous FePO 4 nanosphere@Graphene (FePO 4 @RGO) synthesized via a one-step hydrothermal method. Electrochemical experiments demonstrate that the formation of a mesoporous structure and covered by graphene can address the drawbacks of pure FePO 4 for electroconductivity and ion diffusion rates. When used in desalination, the novel design enhances the desalination capacity, and the rates increase by 60% (50.13–85.94 mg/g) and 180% (0.079–0.24 mg/g/s), respectively, while energy consumption decreases by 25%. The production cost of the material was calculated, and implies that this material has potential for use in large-scale desalination.
研究领域:
环境科学
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材料科学
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水处理
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
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2021
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IF 5.64
Wang Zhihua,Li Qiuke,Li Jinze,Li Jiawei,Shang Lu,Chou Shuli,Lyu Yinfeng,Shan Anshan
Antibiotic resistance is emerging as a hot issue with the abuse and overuse of antibiotics, and the shortage of effective antimicrobial agents against multidrug resistant bacteria creates a huge problem to treat the threatening nosocomial skin and soft tissue infection. Antimicrobial peptides (AMPs) exhibite enormous potential as one of the most promising candidates of antibiotic to fight against pathogenic infections because of its unique membrane penetration mechanism to kill pathogens, whereas the clinical application of AMPs still faces the challenges of production cost, stability, safety, and design strategy. Herein, a series of Trp-rich peptides was designed following the principle of paired Trp plated at the ith and ith+4 position on the backbone of peptides, based on the template (VKKX)4, where X represents W, A, or L, to study the effect of intramolecular aromatic interactions on the bioactivity of AMPs. Through comparing the antimicrobial performance, hemolysis, cytotoxicity, and stability, VW5 which is equipped with the characters of direct antimicrobial efficacy (GM=1.68μM) and physical destruction of bacterial membrane (SEM and electron microscopy) stood out from the engineering peptides. VW5 also performed well in mice models, which could significantly decrease the bacterial colony (VW5 vs infection group, 12.72±2.26 vs 5.52±2.01×109CFU/abscess), the area of dermo-necrosis (VW5 vs infection group, 0.74±0.29 vs 1.86±0.98mm2) and the inflammation cytokine levels at the abscess site without causing toxicity to the skin. Overall, this study provides a strategy and template to diminish the randomness in the exploration and design of novel peptides.
研究领域:
药理学
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微生物学
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传染病学
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分子生物学
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
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