Food Bioscience
·
2024
·
IF 4.8
Yufei Liu,Qingxi Ren,Zhilei Zhou,Zhongwei Ji,Dongliang Ren,Yi Yang,Jian Mao
Baijiu, a distilled Chinese spirit, is one of the most popular alcoholic beverages. However, excessive drinking of Baijiu may cause severe hangover symptoms, including headache, reduced appetite, and dizziness. Herein, we aimed to characterize various clinical hangover symptoms induced by binge consumption of Baijiu samples with different post-drinking qualities in a BALB/c mouse model. Baijiu sample with higher concentrations of alcohols and aldehydes resulted in more severe hangovers in population. Mice administered with the low post-drinking quality Baijiu exhibited altered neurobiochemical parameters and impaired motor performance 10 h after gavage dose of 4.3 g ethanol/kg body weight, including decreased brain 5-hydroxytryptamine, sucrose preference, increased serum endothelin and gait ataxia. Correlation analysis further revealed that these mouse indices were strongly correlated with hangover symptoms in population, and profound correlations were observed between mice indicators and Baijiu congeners. These results indicated that assessing these mouse indices at 10 h after administration can effectively discriminate Baijiu with different hangover effects, and high concentrations of congeners, such as alcohols and aldehydes, may be responsible for the varying post-drinking qualities of Baijiu.
研究领域:
毒理学
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神经科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Zhiying Zhang,Ting Pan,Chunrong Liu,Xiaoyun Shan,Zeqiong Xu,Huachang Hong,Hongjun Lin,Jianrong Chen,Hongjie Sun
The widespread use of cyclophosphamide (CP) in medical treatment had caused ubiquitous contamination in the environment. To data, many studies have been carried out on the toxic effect of CP. However, among these toxic effects of CP, which are the most sensitive remains unclear. Present study aimed to investigate the toxicity of CP on mice and evaluate the sensitivity of physiological-biochemical parameters upon exposure of mice to CP. Results showed that as compared with the control group, CP caused significant reduction in body weight (p < 0.01), spleen coefficient (p < 0.01), leukocyte density (p < 0.01) and alanine transaminase (ALT) in kidney (p < 0.01); However superoxide dismutase (SOD), malondialdehyde (MDA), ALT in liver and creatinine (Cr) in kidney significantly (p < 0.05) increased. Among the suppressed physiological and biochemical parameters, the sensitivity to CP toxicity was generally ranked as body weight > leukocyte density > ALT in kidney > spleen coefficient; while among the stimulated parameters, the sensitivity was ranked as MDA (liver) > Cr (kidney) > ALT (liver). Overall, the most sensitive parameters to CP toxicity may be associated with growth, immune system and the normal function of liver and kidney.
研究领域:
毒理学
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生物化学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
Biomed Research International
·
2022
·Vol. 2022
·期 1
·1254824
·
IF 3.246
Jielun Yu,Lichun Zhang,Xiaoshuang Li,Kaixuan Lv,Shiyu Sun,Weihua Wu,Lifeng Ping,Guifang Guo,Wei Tan,Shoudong Guo,Kezhou Wang,Aihua Zhao,Nana Yang
Background . This study was designed to investigate differences in biochemical parameters between mouse and humans after paraquat (PQ) poisoning and develop a suitable animal model for studying organ damage after PQ poisoning. The prognostic factors of PQ-poisoned patients were further analyzed. Methods . Thirty C57BL/6J mice were randomly divided into five groups (control, sham, and 3 PQ doses), and the mouse model was established by intragastric administration of PQ. Physiological indexes such as the body weight, mental state, and mortality rate were observed. Biochemical parameters were analyzed 24 h after PQ poisoning. We also performed a retrospective analysis of clinical data from 29 patients with PQ poisoning admitted to the Emergency Department of the Affiliated Hospital of Taishan Medical College between April 2016 and February 2018. Biochemical parameters were compared between the mouse model and patients with PQ poisoning. Results . In the PQ poisoning mouse model, the lethal dose group PQ360 showed remarkable increases in serum levels of potassium (K + ), carbon dioxide (CO 2 ), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) compared with the nonlethal dose PQ100 and PQ200 groups. The biochemical results of the patients showed that K + and Cl - levels were significantly reduced in the death group compared to the survival group. Levels of ALT, AST, blood urea nitrogen (BUN), and amylase were higher, and the neutrophil-to-lymphocyte ratio (NLR) was increased in the death group compared with the survival group. Conclusions . The combination of age, PQ dosage, K + , Cl - , BUN, ALT, AST, amylase, and NLR can be used to more accurately predict the outcome of patients with PQ poisoning. C57 mice are an appropriate animal model to study liver and kidney functions following PQ exposure.
研究领域:
毒理学
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医学
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实验动物学
✓ 已验证
DOI
高影响因子
AQUACULTURE NUTRITION
·
2017
·Vol. 23
·期 5
·1076-1085
·
IF 1.665
Q. Zou,Y. Huang,J. Cao,H. Zhao,G. Wang,Y. Li,Q. Pan
The present research evaluated the effects of four feeding stimulants on feed intake (FI), growth performance, body composition, serum biochemical parameters, digestive enzyme activities and appetite-related gene expression for juvenile GIFT tilapia. Five experimental high plant-based diets (with 0, 0.4 g kg −1 dimethyl-β-propiothetin (DMPT), 0.6 g kg −1 dimethylthetin (DMT), 1.8 g kg −1 tryptophan (Trp) and 6 g kg −1 betaine (Bet), respectively supplemented) were fed to juvenile GIFT tilapia ( Oreochromis sp .) (3.34 ± 0.01 g) for 8 weeks. The results showed that FI was significantly increased by Trp and DMT. The highest specific growth rate (SGR) and protein efficiency ratio (PER) was observed for fish fed Bet diet, followed by DMT. Serum triglyceride was significantly increased by DMPT. Fish fed Bet diet showed significantly higher intestinal protease, liver protease and intestinal amylase activities. The neuropeptide Y (NPY) mRNA expression in brain was significantly improved by Trp, and ghrelin mRNA expression in stomach was significantly increased by DMT. It is concluded that supplementation of Trp or DMT could act as an effective feeding stimulants (FS) in high plant-based diets for juvenile GIFT tilapia, which might be associated with the relatively higher orexigenic (NPY or ghrelin) genes expression, and supplementation of Bet could improve SGR, PER and reduce feed conversion ratio (FCR) of juvenile GIFT tilapia.
研究领域:
水产养殖学
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营养学
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分子生物学
✓ 已验证
DOI
高影响因子
CEREAL CHEMISTRY
·
2022
·Vol. 100
·期 2
·393-413
·
IF 2.534
Guangbiao Gao,Jianzhang Wu,Zheng Wei,Xingjun Li
Background and Objectives Cold plasma (CP) treatment is a promising way to remove mycotoxins from cereal kernels, although its effects on nutrient contents remain unclear. This study evaluates the biochemical parameters, gelatinization properties, and fatty acid profile of Chinese hard-white wheat flour treated with low-pressure radio-frequency (RF) CP (13.56 MHz, 140 Pa, helium or oxygen, 120 W, 0–180 s, 70 g sample). Findings 120 W oxygen and helium plasma respectively required 90 and 120 s treatment to remove 50% deoxynivalenol (DON) from hard-white wheat flour samples with 1.22 μg/g DON. A General Linear Model–Univariate analysis shows that oxygen and helium plasma both significantly increased water absorption rate, conductivity, and contents of reducing sugars, amylose, soluble protein, and saturated fatty acids in wheat flours but decreased the contents of soluble amino acids, total, unsaturated, mono-unsaturated and poly-unsaturated fatty acids, and enthalpy of gelatinization, and kept gelatinization peak temperature. At the same treatment time, helium plasma induced a higher water absorption rate, conductivity, and contents of pentosan and sulfhydryl groups in wheat flours than oxygen plasma. Oxygen plasma induced more reductions in mono- and poly-unsaturated fatty acids in wheat flours than helium plasma. Compared with the untreated samples, helium plasma increased the values of water SRC, NaHCO 3 SRC, and sucrose SRC, but decreased the lactic acid SRC, suggesting that the flour functional polymers were modified by helium plasma treatment, but oxygen plasma kept these four SRC values and did not significantly alter the functional polymers in wheat flour. Conclusion The treatment with low-pressure RF CP can increase hydrophilicity, and unsaturated fatty acid oxidation in wheat flours while kept starch gelatinization, and helium plasma can modify the flour's functional polymers. Significance and Novelty The suitable helium CP treatment (low-pressure RF, 120 W, and 90–120 s) is a promising way to reduce the contaminants of wheat flours and improve their functional properties.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2017
·
IF 3.743
Peng Xu,Ledan Huang
Populations of many amphibian species are declining worldwide in part because of pesticide contamination. As a surface water contaminant, α-cypermethrin may have severe ecological impacts on amphibians. Here, we examined the acute toxicity of α-cypermethrin enantiomers to dark-spotted frog Rana nigromaculata tadpoles at 24, 48, 72 and 96 h, finding that the tadpoles were indeed sensitive to α-cypermethrin. The (S)-(1R, 3R)-enantiomer was approximately 29 times more toxic than the (R)-(1S, 3S)-enantiomer at 96 h. A significant delayed growth in R. nigromaculata tadpoles after exposure to 0.5 µg L −1 of S-(1R, 3R)-cypermethrin was observed. Additionally, increased superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) and malondialdehyde (MDA) levels indicate the presence of oxidative stress in the tadpoles. Further, tadpoles exposed to sublethal concentrations of α-cypermethrin enantiomers exhibited enantioselective growth and oxidative damage. Bioaccumulation experiments showed that the tadpoles could rapidly accumulate α-cypermethrin. The (R)-(1S, 3S)-enantiomer was preferentially accumulated over the (S)-(1R, 3R)-enantiomer, and it was also eliminated more quickly, as evidenced in the subsequent depuration experiments.
研究领域:
环境科学
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毒理学
·
生态学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2018
·Vol. 9
·期 1
·1-8
·
IF 12.353
Zhang Ting,Liu Gang-Qin,Leong Weng-Hang,Liu Chu-Feng,Kwok Man-Hin,Ngai To,Liu Ren-Bao,Li Quan
Diamond nitrogen-vacancy (NV) center-based magnetometry provides a unique opportunity for quantum bio-sensing. However, NV centers are not sensitive to parameters such as temperature and pressure, and immune to many biochemical parameters such as pH and non-magnetic biomolecules. Here, we propose a scheme that can potentially enable the measurement of various biochemical parameters using diamond quantum sensing, by employing stimulus-responsive hydrogels as a spacing transducer in-between a nanodiamond (ND, with NV centers) and magnetic nanoparticles (MNPs). The volume phase transition of hydrogel upon stimulation leads to sharp variation in the separation distance between the MNPs and the ND. This in turn changes the magnetic field that the NV centers can detect sensitively. We construct a temperature sensor under this hybrid scheme and show the proof-of-the-principle demonstration of reversible temperature sensing. Applications in the detection of other bio-relevant parameters are envisioned if appropriate types of hydrogels can be engineered.
研究领域:
量子传感
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材料科学
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生物物理
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL BIOLOGY
·
2020
·
IF 2.971
Xiaoman Zhang,Jie Feng,Shaofeng Su,Lei Huang
Context Camellia nitidissima Chi (Theaceae) is an evergreen shrub, the leaves of which are used in many medicinal applications.Objective To characterize the chemical composition of a 10% aqueous ethanol extract of C. nitidissima leaves (CNE), and to explore the protective effect of the extract against acute liver injury (ALI) in rats.Materials and methods Male Sprague-Dawley rats were divided into six groups (n = 10): control and negative (0.5% CMC-Na, 5 mL/kg/d), thiopronin (20 mg/kg/d) and CNE (40, 80 and 160 mg/kg/d). All groups were treated for seven consecutive days, and then, except for the control, carbon tetrachloride was administered intraperitoneally. The biochemical parameters, mRNAs, and proteins were analyzed using enzyme-linked immunoassays kits, quantitative polymerase chain reaction and western blot. Chemical components were identified using mass spectroscopy, and the phenol and flavonoid content determined by ultraviolet spectrophotometry.Results Pre-treatment with CNE (160 mg/kg) attenuated the pathological changes in liver tissues and decreased alanine transaminase (62 and 60%), aspartate transaminase (49 and 53%) and malondialdehyde (35 and 42%) levels in serum and liver tissues. Moreover, CNE reduced the concentrations of reactive oxygen species (55%), tumour necrosis factor-α (26%), interleukin-1β (19%) and IL-6 (19%) and blocked the nuclear translocation of p65. Pre-treatment with CNE increased anti-heme oxygenase-1 (40%), superoxide dismutase (108%) and glutathione (97%) levels through upregulating nuclear factor erythroid-2-related factor 2. Twelve compounds were detected; the content of phenols and flavonoids was determined as 34.474 ± 1.026 and 15.228 ± 0.422 mg/g crude drug in CNE, respectively.Discussion and conclusions These results suggested that CNE is a promising agent for functional food and hepatoprotective drug against ALI.
研究领域:
药理学
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毒理学
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分子生物学
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药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2017
·Vol. 82
·期 3
·757-764
·
IF 1.815
Weimin Tang,Lu Jin,Lianghua Xie,Juqing Huang,Nan Wang,Bingquan Chu,Xulin Dai,Yu Liu,Rui Wang,Ying Zhang
en This link goes to a English section pt This link goes to a English section Maca ( Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo . The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model. MP is an acidic heteropolysaccharide with an average molecular weight ( M w ) of 793.5 kDa. It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %). The findings revealed that MP contained β-1,3-Gal p (A), β-1,3-Glc p , and α-1, 3-Man p linked alternatingly to form a backbone (5:4:1). MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice ( P < 0.05) and improve the serous biochemical parameters of mice. Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities ( P < 0.05) and decreased lactate dehydrogenase activity ( P < 0.01). High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde ( P < 0.05). MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca. Practical Application en This link goes to a English section pt This link goes to a English section The antifatigue activity of polysaccharides isolated from maca ( Lepidium meyenii Walp) was investigated in this study. Polysaccharides, as a major component of maca (above 10% in dry weight), could be explored as a natural antifatigue functional food in the future.
研究领域:
药理学
·
营养保健品
·
生物化学
·
功能性食品
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
·
2015
·
IF 3.262
Zhangjian Chen,Yun Wang,Lin Zhuo,Shi Chen,Lin Zhao,Xianguo Luan,Haifang Wang,Guang Jia
Titanium dioxide nanoparticles (TiO 2 NPs) have been widely used in various consumer products, especially food and personal care products. Compared to the well-characterized adverse cardiovascular effect of inhaled ambient ultrafine particles, research on the health response to orally administrated TiO 2 NPs is still limited. In our study, we performed an in vivo study in Sprague-Dawley rats to understand the cardiovascular effect of TiO 2 NPs after oral intake. After daily gastrointestinal administration of TiO 2 NPs at 0, 2, 10, 50 mg/kg for 30 and 90 days, heart rate (HR), blood pressure, blood biochemical parameters and histopathology of cardiac tissues was assessed to quantify cardiovascular damage. Mild and temporary reduction of HR and systolic blood pressure as well as an increase of diastolic blood pressure was observed after daily oral administration of TiO 2 NPs for 30 days. Injury of cardiac function was observed after daily oral administration of TiO 2 NPs for 90 days as reflected in decreased activities of lactate dehydrogenase (LDH), alpha-hydroxybutyrate dehydrogenase (HBDH) and creatine kinase (CK). Increased white blood cells count (WBC) and granulocytes (GRN) in blood as well as increased concentrations of tumor necrosis factor α (TNF α) and interleukin 6 (IL-6) in the serum indicated inflammatory response initiated by TiO 2 NPs exposure. It was hypothesize that cardiac damage and inflammatory response are the possible mechanisms of the adverse cardiovascular effects induced by orally administrated TiO 2 NPs. Data from our study suggested that even at low dose of TiO 2 NPs can induce adverse cardiovascular effects after 30 days or 90 days of oral exposure, thus warranting concern for the dietary intake of TiO 2 NPs for consumers.
研究领域:
毒理学
·
心脏病学
·
纳米技术
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）