BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2024
·Vol. 114
·期 1
·1-8
·
IF 2.7
Wang Jun,Li Meng,Gu Lei,Sun Yunfei,Zhu Xuexia,Yang Zhou
Atrazine is a predominant herbicide globally, and its residues are commonly found in natural water bodies due to its extensive use. Atrazine is known for its detrimental effects on the reproductive abilities of aquatic plants and animals. Our study explored the impact of maternal exposure to atrazine on the survival and performance of offspring using the water flea Daphnia magna as a model organism. We investigated how the life-history traits and population dynamics of maternal D. magna and their offspring are influenced by atrazine. We hypothesized that exposure to atrazine in mothers would negatively affect the survival and performance of their offspring. The findings revealed that short-term exposure to atrazine adversely affects fitness-related traits, manifesting as decreased survivorship and reduced fecundity. Offspring from atrazine-exposed mothers displayed a limited recovery in fitness; no mortality was observed during the 14-day experiment, yet fecundity remained significantly reduced. At the population level, maternal exposure to atrazine led to a decreased population carrying capacity. The notable maternal effects on offspring reproduction underscore the long-term toxicity of atrazine to D. magna populations, suggesting prolonged consequences even after the cessation of atrazine pollution. This study underscores the transgenerational effects of atrazine, highlighting its persistent threat to D. magna populations.
研究领域:
生态毒理学
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环境科学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Shilong Ma,Yang Yang,Zhongmin Fu,Qingyun Diao,Mengyue Wang,Qihua Luo,Xing Wang,Pingli Dai
Tropilaelaps mercedesae is not only a major threat to honey bees in Asia but also a potential risk to global apiculture due to trade. Imidacloprid is a systemic insecticide that negatively affects individual bees. Moreover, the health of honey bees may be threatened by imidacloprid exposure and T. mercedesae infestation. We studied the effects of T. mercedesae and imidacloprid on the survival, food consumption and midgut bacterial diversity of Apis mellifera in the laboratory . Illumina 16S rRNA gene sequencing was used to determine the bacterial composition in the honey bee midgut. T. mercedesae decreased survival in parasitized honey bees compared with nonparasitized honey bees, but there was no significant difference in food consumption. The imidacloprid 50 μg/L diet significantly decreased syrup consumption of A. mellifera compared with the control diet. The combination of T. mercedesae infestation and imidacloprid 50 μg/L exposure reduced survival and increased pollen consumption of A. mellifera . T. mercedesae infestation or a combination of T. mercedesae infestation and exposure to 25 μg/L imidacloprid affected the midgut bacterial composition of honey bees. T. mercedesae infestation and imidacloprid exposure may reduce the survival and affect honey bee health.
研究领域:
昆虫学
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微生物学
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生态毒理学
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PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2022
·
IF 4.52
Ziyu Li,Shaoting Yin,Jing Zhang,Gengsi Xi
Aspartame is a food additive that is widely used in the food industry. Previous data have shown that aspartame is toxic to humans and animals. However, there are few reports on the effect of aspartame on social insects living in the soil. The present study was designed to evaluate aspartame toxicity for Polyrhachis vicina Roger. Five dosages of aspartame were fed to the workers and 40 mg/kg (acceptable daily intake, ADI) aspartame was chosen to feed the 4th instar larvae, pupae, females and males in P. vicina . The results showed that the effect of aspartame on the survival rate of workers was in a time and dose dependent manner. The survival rate of 4th instar larvae, pupae and males decreased at 40 mg/kg aspartame. Meanwhile, we found that aspartame toxicity can cause weight and morphological changes. These changes may be related to the abnormal gene expression of Ecdysone receptor , Tailless and Extradenticle of P. vicina ( PvEcR , PvTll and PvExd ) resulting from aspartame treatment. The present study confirms the effect of aspartame toxicity on P. vicina even at the ADI dosage.
研究领域:
昆虫学
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毒理学
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分子生物学
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PubMed
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
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2020
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IF 4.419
Bin Li,Guoqiang Sun,Daquan Sun,Tong Lu,Jianmin Ma,Yue Deng
Microcapsule technology exhibits great potential in improving the healing efficiency of asphalt mixture and prolonging the service life of the pavement. The capsule should be kept intact in the mixing and compaction process of asphalt mixture, whereas broken to release the healing agents in time when cracks encounter it. This paper aims to investigate the survival and activation behavior of microcapsules in asphalt mixture during construction and service period. By means of the in situ polymerization method, the microcapsules of various sizes were prepared and their particle size distribution, core material content, shell permeability, and other properties were characterized. Based on the quantitative analysis principle of Fourier transform infrared spectrometer (FTIR), the influence of microcapsule size and mixing temperature on the survival rate of microcapsules was analyzed. Meanwhile, the morphology and size distribution of surviving microcapsules was obtained via fluorescence microscope (FM). In addition, the morphologies of microcapsules before and after the bitumen bond strength (BBS) test and dynamic shear rheological (DSR) test were observed by FM to examine the releasing behavior of microcapsules. Finally, the fatigue-rest-fatigue test was performed to investigate the influence of microcapsule size on the healing efficiency of aged asphalt mastic. The results showed that the survival rate could be improved by reducing the construction temperature and the microcapsules size. The loss of microcapsules was more likely to happen in the mixing process rather than in the compaction process. Microcapsules with larger sizes were easier to be activated to remarkably enhance the healing efficiency of the aged asphalt mastic. Notably, the microcapsules with appropriate size are recommended to be added to the mixtures to ensure that the microcapsules survive in the construction process and are activated when cracks appeared.
研究领域:
土木工程
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材料科学
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DOI
高影响因子
JOURNAL OF FISH DISEASES
·
2022
·Vol. 46
·期 3
·215-227
·
IF 2.58
Ziqing Zhong,Xiping Wu,Mindong Bai,Xiaodian Huang,Qilin Zheng,Chunxiang Ai
Cryptocaryon irritans causes one of the most serious diseases in various wild and cultured marine fish, leading to mass mortality and economic loss. In this study, hydroxyl radical (•OH) solution produced by strong ionization discharge combined with water jet cavitation effect was injected into orange-spotted grouper ( Epinephelus coioides ) aquaculture tanks for C. irritans control. The results showed that all C. irritans theronts were inactivated by •OH solution at concentrations of 0.5 mg/L within 2 min. •OH could induce alteration of shape, the absence of motility and macronucleus dispersion in theronts. A possible explanation was that the macronucleus of C. irritans might be damaged by •OH; as a result, its metabolism and life activities were disturbed. The •OH treatment increased the survival rate of E. coioides challenged with C. irritans from 64.7 ± 8.0% (mean ± SD) to 100% and reduced their infection intensity significantly. Stress response biomarkers such as malonaldehyde, glutathione, glutathione peroxidase, superoxide dismutase (SOD) and catalase levels in the gills of E. coioides at different time points were analysed. The SOD activity in the •OH group first decreased and then recovered to the initial level at the end of the experiment. The other stress response biomarkers had no significant difference from that in the uninfected control group after •OH treatment. Additionally, the gill of E. coioides in the •OH group exhibited slight and reversible transformation compared with the uninfected control group. Compared with •OH treatment, chlorine dioxide and formalin treatment reduced the survival rate, induced oxidative damage and changed the histological gill structure in E. coioides . In conclusion, •OH could be applied effectively to control C. irritans infection without affecting the normal physiological condition of E. coioides .
研究领域:
水产养殖学
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寄生虫学
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DOI
PubMed
高影响因子
Antibiotics-Basel
·
2022
·Vol. 11
·期 11
·1660
·
IF 5.222
Yan Wang,Lanyu Liu,Qiping Wu,Qiufen Yin,Feifan Xie
Background: The pharmacokinetic/pharmacodynamic (PK/PD) index of carbapenems that best correlates with in vivo antimicrobial activity is percent time of dosing interval in which free drug concentration remains above MIC (%fT > MIC), while the magnitudes of the PK/PD index of carbapenems remains undefined in critically ill sepsis patients.Methods: A sepsis rat model was first developed by comparing the survival outcomes after intraperitoneal injection of different inoculum size (1–10 × 107CFU) ofPseudomonas aeruginosaATCC9027 (MIC = 0.125 mg/L) in neutropenic rats. The PK characteristics of the model drug meropenem in the developed sepsis rat model was then evaluated, and PK modeling and simulation was applied to design meropenem dosing regimens attaining various PD targets (40%fT > MIC, 100%fT > MIC, and 100%fT > 4 × MIC). The microbiological response and survival outcomes for different meropenem treatment regimens were investigated in the rat sepsis model (n= 12 for each group).Results: The optimal inoculum for the rat sepsis model was 1 × 107CFU ofPseudomonas aeruginosaATCC9027. A one-compartment model with first-order absorption best described the PK of meropenem in sepsis rats. Pronounced survival prolongation and lower hazard risk were observed in the treatment groups of 50 or 75 mg/kg/q2.4h (100%fT > MIC) and 75 mg/kg/q2h (100%fT > 4 × MIC) compared to the 75 mg/kg/q6h (40%fT > MIC) group, while meropenem groups with PD targets of 100%fT > MIC and 100%fT > 4 × MIC showed comparable survival curves. Microbiological response for different PD targets is inconclusive due to irregular bacterial counts in blood samples.Conclusions: The PD target of 40%fT > MIC is suboptimal for sepsis rats, and the aggressive 100%fT > 4 × MIC target does not provide a survival benefit against the target of 100%fT > MIC.Keywords:pharmacokinetics;PK/PD;survival;meropenem;sepsis rat model
研究领域:
药理学
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感染病学
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PubMed
高影响因子
Journal of Oncology
·
2021
·
IF 4.375
Jie Mei,Tiejun Wang,Shaojie Zhao,Yan Zhang
Osthole (OST) is a plant-derived compound that can inhibit the proliferation of tumor cells and has a tumor-suppressive effect in multiple types of cancers. However, the mechanisms of OST-mediated breast cancer (BrCa) inhibition were still largely unknown. In this study, we made full use of the GSE85871 dataset to identify potential targets of OST in BrCa via multiple bioinformatics analysis. Next, a series of in vitro experiments were conducted to check the role of GNG7 in BrCa and the relationship between OST and GNG7. Through a series of bioinformatics analyses, GNG7 was identified as a potential target of OST, which could be significant upregulated by OST exposure in BrCa cells. Besides, GNG7 was lowly expressed in BrCa tissues compared with normal breast tissues, and BrCa patients with low GNG7 expression had shorter overall survival (OS) and relapse-free survival (RFS) compared with those with high GNG7 expression. Moreover, GNG7 silencing significantly enhanced cell proliferation and inhibited apoptosis, and exogenous overexpression of GNG7 showed reverse effects on BrCa cells. Last but not least, GNG7 inhibition could notably rescue OST-mediated cytotoxic effects. In summary, we identified GNG7 as a novel target for OST in BrCa and a potential tumor suppressor. Thus, OST could be therapeutically beneficial for BrCa through a GNG7-dependent mechanism.
研究领域:
癌症生物学
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分子生物学
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药理学
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生物信息学
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DOI
PubMed
高影响因子
STAR Protocols
·
2023
·
IF 0
Jie Zhang,Xiao-Tong Lin,Lei Fang,Chuan-Ming Xie
Summary FBXO45, an E3 ubiquitin ligase highly expressed in liver tumors, is positively correlated with poor survival of hepatocellular carcinogenesis (HCC) patients, but whether FBXO45 drives HCC tumorigenesis remains largely unclear. Here, we describe a protocol that shortens the observation period for HCC tumorigenesis to assess the effects of FBXO45 in a DEN/CCl 4 -induced HCC mouse model. We describe steps for chemical induction of HCC in FBXO45-overexpressing mice, followed by tissue collection and pathology assessment via quantitative real-time PCR, histology, and immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Lin et al. (2021). 1
研究领域:
癌症生物学
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分子生物学
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病理学
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DOI
PubMed
Experimental and Therapeutic Medicine
·
2019
·Vol. 18
·期 4
·3117-3124
·
IF 1.448
Qiurong Zhang,Xiao Wu,Jing Cao,Feng Gao,Kun Huang
A total of 133 patients with acute myeloid leukemia (AML) were enrolled in the current study and were subdivided into 4 groups: 34 harboring DNA methyltransferase 3 α (DNMT3A) + fms related tyrosine kinase 3‑internal tandem duplication (FLT3‑ITD) mutations, 37 harboring only FLT3‑ITD mutation, 32 harboring only DNMT3A mutation and 30 harboring no mutations in DNMT3A and FLT3‑ITD (control). Patients in all groups were administered daunorubicin and cytarabine chemotherapy regimens. The rates of complete remission (CR), 1‑year relapse (RR) and 3‑year overall survival (OS) were compared. Patients in the DNMT3A + FLT3‑ITD mutation group exhibited higher proportions of peripheral white blood cells (WBCs) and myeloid progenitor cells compared with those in DNMT3A mutation only, FLT3‑ITD mutation only and control groups (P<0.05). The rates of CD15+ and HLA‑DR+ in the DNMT3A + FLT3‑ITD mutation and DNMT3A mutation only groups were significantly higher than those in the FLT3‑ITD mutation only and control groups (P<0.05); in addition, the rate of CD38+ in the DNMT3A + FLT3‑ITD mutation and FLT3‑ITD mutation only groups was significantly higher compared with that in the DNMT3A mutation only and control groups (P<0.05). The overall chemotherapy effectiveness rate, CR, 1‑year RR and the 3‑year OS rates of patients in the DNMT3A + FLT3‑ITD mutation group were significantly worse compared with FLT3‑ITD mutation only, DNMT3A mutation only and control groups (P<0.05). The results of this study indicated that increased mutation rates in DNMT3Α and FLT3‑ITD may be associated with increased WBC and myeloid progenitor cell counts, an inferior chemotherapy efficacy and prognosis, a lower CR rate, and higher 1‑year RR and mortality rate.
研究领域:
血液学
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肿瘤学
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分子生物学
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Siyu Xu,Yu Lei,Mulin Wei,Zhixiang Fang,Yue Cheng,Gang Zhao,Zhongrong Chen
Oocyte cryopreservation plays a crucial role in safeguarding female fertility in clinical practice. However, commonly used cryopreservation methods involving tedious and time-consuming procedures, as well as uncontrolled variability between operators, which can easily lead to cell damage. Here, a novel method for cryopreserving oocytes using a transparent alumina ceramics-based microfluidic chip is developed, which provides an integrated platform for cryoprotectants (CPAs) loading/unloading, as well as freeze-thaw process and storage. The cryopreservation protocol is optimized to significantly simplify the overall process, reduce CPA osmotic damage, and achieve high-performance oocyte cryopreservation. The survival rate of oocyte cryopreserved with this method reached 86.23%. In comparison to conventional method, the recovered oocytes exhibit structural stability, functional integrity, genetic normality and higher developmental capacity. The study offers a low-cost, easy-to-operate, high-performance, on-chip semi-automatic cryopreservation technique for oocytes, which is of great significance for standardizing and automating the cryopreservation of female fertility, and has important potential for translational applications.
研究领域:
生殖医学
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生物工程
·
材料科学
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DOI
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6
Zi-Qiang Feng,Jing Ding,Min-Zhen Zhu,Wei-Song Xie,Rui-Chen Liu,Si-Si Liu,Si-Meng Liu,Ming-Jia Yu,Xin-Hong Zhu,Jian-Hua Liang
Recently, some inhibitors of soluble epoxide hydrolase (sEH) showed limited potential in treating sepsis by increasing survival time, but they have unfortunately failed to improve survival rates. In this study, we initially identified a new hit 11D , belonging to a natural skeleton known as stilbene and having an IC 50 of 644 nM on inhibiting murine sEH. Natural scaffold-based sEH inhibitors are paid less attention. A combination of structure-activity relationships (SARs)-guided structural optimization and computer-aided skeleton growth led to a highly effective lead compound 70P (IC 50 : 4.0 nM). The dose-response study indicated that 70P (at doses of 0.5–5 mg/kg, ip.) significantly increased survival rates and survival time by reducing the levels of the inflammatory factors TNF-α and IL-6 in the liver. Interestingly, 70P exhibited much higher accumulation in the liver than in plasma (AUC ratio: 175). In addition, 70P exhibits equal IC50 value (1.5 nM) on inhibiting human sEH as EC5026 (1.7 nM). In conclusion, the natural scaffold-extended sEH inhibitor 70P has the potential to become a new promising lead for addressing the unmet medical need in sepsis treatment, which highlighted the importance of natural skeleton in developing sEH inhibitors.
研究领域:
药理学
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医学
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计算化学
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DOI
PubMed
高影响因子
CELL AND TISSUE RESEARCH
·
2023
·
IF 3.6
Yuan Haojie,Yu Junbo,Liu Chun,Zhao Heyan,Xue Jianhua,Liu Jiajia,Yang Yang
This study aims to discuss the role of exosomes KCNQ10T1 derived from bone marrow mesenchymal stem cells (BMMSCs) in sepsis and to further investigate its potential molecular mechanisms. Exosomes extracted from BMMSCs are identified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blot. Fluorescence labeling is applied to detect the internalization of exosomes in receptors. The proliferation ability, migration ability, and invasion ability of HUVECs are determined by CCK-8, EdU, wound healing, and Transwell. The levels of inflammatory cytokines in sepsis cells are quantitatively detected by ELISA. Kaplan–Meier survival curve is used to describe the overall survival. RT-qPCR is used to detect mRNA expression of related genes. Bioinformatics analysis is performed to search the downstream target of KCNQ1OT1 and miR-154-3p and the interaction is verified by luciferase reporter assay. Exosomes derived from BMMSCs alleviated the toxicity in sepsis cell models and animal models. In mice with septic cell models, exosomal KCNQ10T1 was down-regulated and associated with lower survival. Overexpression of KCNQ10T1 inhibited the proliferation and metastasis of LPS-induced HUVECs. Further research illustrated that miR-154-3p was the downstream target gene of KCNQ1OT1 and RNF19A was the downstream target gene of miR-154-3p. Importantly, functional research findings indicated that KCNQ1OT1 regulated sepsis progression by targeting miR-154-3p/RNF19A axis. Our study demonstrates that the exosomal KCNQ1OT1 suppresses sepsis via mediating miR-154-3p/RNF19A, which provides a latent target for sepsis treatment.
研究领域:
分子生物学
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干细胞研究
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免疫学
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细胞生物学
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医学遗传学
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DOI
PubMed
高影响因子
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