BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2024
·Vol. 114
·期 1
·1-8
·
IF 2.7
Wang Jun,Li Meng,Gu Lei,Sun Yunfei,Zhu Xuexia,Yang Zhou
Atrazine is a predominant herbicide globally, and its residues are commonly found in natural water bodies due to its extensive use. Atrazine is known for its detrimental effects on the reproductive abilities of aquatic plants and animals. Our study explored the impact of maternal exposure to atrazine on the survival and performance of offspring using the water flea Daphnia magna as a model organism. We investigated how the life-history traits and population dynamics of maternal D. magna and their offspring are influenced by atrazine. We hypothesized that exposure to atrazine in mothers would negatively affect the survival and performance of their offspring. The findings revealed that short-term exposure to atrazine adversely affects fitness-related traits, manifesting as decreased survivorship and reduced fecundity. Offspring from atrazine-exposed mothers displayed a limited recovery in fitness; no mortality was observed during the 14-day experiment, yet fecundity remained significantly reduced. At the population level, maternal exposure to atrazine led to a decreased population carrying capacity. The notable maternal effects on offspring reproduction underscore the long-term toxicity of atrazine to D. magna populations, suggesting prolonged consequences even after the cessation of atrazine pollution. This study underscores the transgenerational effects of atrazine, highlighting its persistent threat to D. magna populations.
研究领域:
生态毒理学
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环境科学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Shilong Ma,Yang Yang,Zhongmin Fu,Qingyun Diao,Mengyue Wang,Qihua Luo,Xing Wang,Pingli Dai
Tropilaelaps mercedesae is not only a major threat to honey bees in Asia but also a potential risk to global apiculture due to trade. Imidacloprid is a systemic insecticide that negatively affects individual bees. Moreover, the health of honey bees may be threatened by imidacloprid exposure and T. mercedesae infestation. We studied the effects of T. mercedesae and imidacloprid on the survival, food consumption and midgut bacterial diversity of Apis mellifera in the laboratory . Illumina 16S rRNA gene sequencing was used to determine the bacterial composition in the honey bee midgut. T. mercedesae decreased survival in parasitized honey bees compared with nonparasitized honey bees, but there was no significant difference in food consumption. The imidacloprid 50 μg/L diet significantly decreased syrup consumption of A. mellifera compared with the control diet. The combination of T. mercedesae infestation and imidacloprid 50 μg/L exposure reduced survival and increased pollen consumption of A. mellifera . T. mercedesae infestation or a combination of T. mercedesae infestation and exposure to 25 μg/L imidacloprid affected the midgut bacterial composition of honey bees. T. mercedesae infestation and imidacloprid exposure may reduce the survival and affect honey bee health.
研究领域:
昆虫学
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微生物学
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生态毒理学
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DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2022
·
IF 4.52
Ziyu Li,Shaoting Yin,Jing Zhang,Gengsi Xi
Aspartame is a food additive that is widely used in the food industry. Previous data have shown that aspartame is toxic to humans and animals. However, there are few reports on the effect of aspartame on social insects living in the soil. The present study was designed to evaluate aspartame toxicity for Polyrhachis vicina Roger. Five dosages of aspartame were fed to the workers and 40 mg/kg (acceptable daily intake, ADI) aspartame was chosen to feed the 4th instar larvae, pupae, females and males in P. vicina . The results showed that the effect of aspartame on the survival rate of workers was in a time and dose dependent manner. The survival rate of 4th instar larvae, pupae and males decreased at 40 mg/kg aspartame. Meanwhile, we found that aspartame toxicity can cause weight and morphological changes. These changes may be related to the abnormal gene expression of Ecdysone receptor , Tailless and Extradenticle of P. vicina ( PvEcR , PvTll and PvExd ) resulting from aspartame treatment. The present study confirms the effect of aspartame toxicity on P. vicina even at the ADI dosage.
研究领域:
昆虫学
·
毒理学
·
分子生物学
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DOI
PubMed
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2020
·
IF 4.419
Bin Li,Guoqiang Sun,Daquan Sun,Tong Lu,Jianmin Ma,Yue Deng
Microcapsule technology exhibits great potential in improving the healing efficiency of asphalt mixture and prolonging the service life of the pavement. The capsule should be kept intact in the mixing and compaction process of asphalt mixture, whereas broken to release the healing agents in time when cracks encounter it. This paper aims to investigate the survival and activation behavior of microcapsules in asphalt mixture during construction and service period. By means of the in situ polymerization method, the microcapsules of various sizes were prepared and their particle size distribution, core material content, shell permeability, and other properties were characterized. Based on the quantitative analysis principle of Fourier transform infrared spectrometer (FTIR), the influence of microcapsule size and mixing temperature on the survival rate of microcapsules was analyzed. Meanwhile, the morphology and size distribution of surviving microcapsules was obtained via fluorescence microscope (FM). In addition, the morphologies of microcapsules before and after the bitumen bond strength (BBS) test and dynamic shear rheological (DSR) test were observed by FM to examine the releasing behavior of microcapsules. Finally, the fatigue-rest-fatigue test was performed to investigate the influence of microcapsule size on the healing efficiency of aged asphalt mastic. The results showed that the survival rate could be improved by reducing the construction temperature and the microcapsules size. The loss of microcapsules was more likely to happen in the mixing process rather than in the compaction process. Microcapsules with larger sizes were easier to be activated to remarkably enhance the healing efficiency of the aged asphalt mastic. Notably, the microcapsules with appropriate size are recommended to be added to the mixtures to ensure that the microcapsules survive in the construction process and are activated when cracks appeared.
研究领域:
土木工程
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材料科学
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DOI
高影响因子
PLoS One
·
2014
·Vol. 9
·期 4
·e94418
·
IF 3.534
Wu Li,Quanju Zhao,Wanyan Deng,Tian Chen,Minqiang Liu,Jianping Xie
Intracellular survival plays a central role in the pathogenesis of Mycobacterium tuberculosis , a process which depends on an array of virulence factors to colonize and replicate within the host. The M. tuberculosis iron regulated open reading frame (ORF) rv3402c , encoding a conserved hypothetical protein, was shown to be up-regulated upon infection in both human and mice macrophages. To explore the function of this ORF, we heterologously expressed the rv3402c gene in the non-pathogenic fast-growing Mycobacterium smegmatis strain, and demonstrated that Rv3402c, a cell envelope-associated protein, was able to enhance the intracellular survival of recombinant M. smegmatis . Enhanced growth was not found to be the result of an increased resistance to intracellular stresses, as growth of the Rv3402c expressing strain was unaffected by iron depletion, H 2 O 2 exposure, or acidic conditions. Colonization of macrophages by M. smegmatis expressing Rv3402c was associated with substantial cell death and significantly greater amount of TNF-α and IL-1β compared with controls. Rv3402c-induced TNF-α and IL-1β production was found to be mediated by NF-κB, ERK and p38 pathway in macrophages. In summary, our study suggests that Rv3402c delivered in a live M. smegmatis vehicle can modify the cytokines profile of macrophage, promote host cell death and enhance the persistence of mycobacterium within host cells.
研究领域:
微生物学
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免疫学
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DOI
高影响因子
CHEMOSPHERE
·
2013
·
IF 3.137
Xiaoyan Zhang,Peiyong Guo,Jinxiu Huang,Xiufu Hou
Suspended particles are a natural component of aquatic ecosystems. This study provides a report on the survival, growth and reproduction of common-scale and nanoscale particles of Daphnia magna Straus exposed to five types of particles (i.e. KN (kaolinite), MN (montmorillonite), MNn (nanoscale MN), NP (natural particles), and NPn (nanoscale NP)). The results of the study show that the suspended particles elicited a dose-dependent toxicity in KN, MN and MNn, with the following toxicity pattern: MN > KN > MNn. On the contrary, NP and NPn did not show any harmful effects on the animals. Instead, NP and NPn, especially NPn, contributed to the survivorship of the animals. The animals were able to survive throughout the 21-day period of bioassays, and when the particle concentration reached 600 mg L −1 , they produced many neonates without any addition of food. This experimental results also indicated that the nanoscale particles were less toxic than the common-scale ones, both for MN and NP. Moreover, the results of the 21-day period of bioassays indicated that when the organisms were exposed to particles of different size scales, they showed different selection patterns for allocating resources. This may be due to the generation of different assimilation and digestion patterns.
研究领域:
生态毒理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FISH DISEASES
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2022
·Vol. 46
·期 3
·215-227
·
IF 2.58
Ziqing Zhong,Xiping Wu,Mindong Bai,Xiaodian Huang,Qilin Zheng,Chunxiang Ai
Cryptocaryon irritans causes one of the most serious diseases in various wild and cultured marine fish, leading to mass mortality and economic loss. In this study, hydroxyl radical (•OH) solution produced by strong ionization discharge combined with water jet cavitation effect was injected into orange-spotted grouper ( Epinephelus coioides ) aquaculture tanks for C. irritans control. The results showed that all C. irritans theronts were inactivated by •OH solution at concentrations of 0.5 mg/L within 2 min. •OH could induce alteration of shape, the absence of motility and macronucleus dispersion in theronts. A possible explanation was that the macronucleus of C. irritans might be damaged by •OH; as a result, its metabolism and life activities were disturbed. The •OH treatment increased the survival rate of E. coioides challenged with C. irritans from 64.7 ± 8.0% (mean ± SD) to 100% and reduced their infection intensity significantly. Stress response biomarkers such as malonaldehyde, glutathione, glutathione peroxidase, superoxide dismutase (SOD) and catalase levels in the gills of E. coioides at different time points were analysed. The SOD activity in the •OH group first decreased and then recovered to the initial level at the end of the experiment. The other stress response biomarkers had no significant difference from that in the uninfected control group after •OH treatment. Additionally, the gill of E. coioides in the •OH group exhibited slight and reversible transformation compared with the uninfected control group. Compared with •OH treatment, chlorine dioxide and formalin treatment reduced the survival rate, induced oxidative damage and changed the histological gill structure in E. coioides . In conclusion, •OH could be applied effectively to control C. irritans infection without affecting the normal physiological condition of E. coioides .
研究领域:
水产养殖学
·
寄生虫学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
·
2019
·Vol. 38
·期 10
·2169-2177
·
IF 3.421
An He,Xinyong Liu,Liang Qu,Yongfei Gao,Jianfeng Feng,Lin Zhu
We exposed zebrafish ( Danio rerio ) to different concentrations of lead and cadmium, and monitored them for survival at 24, 48, 72, and 96 h. Metal toxicity was predicted and compared using the dose–response and general threshold survival models in terms of required data sets, fit performance, and applicability. Environ Toxicol Chem 2019;38:2169–2177. © 2019 SETAC.
研究领域:
环境毒理学
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生态学
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产品关联
DOI
PubMed
高影响因子
Journal of Oncology
·
2021
·
IF 4.375
Jie Mei,Tiejun Wang,Shaojie Zhao,Yan Zhang
Osthole (OST) is a plant-derived compound that can inhibit the proliferation of tumor cells and has a tumor-suppressive effect in multiple types of cancers. However, the mechanisms of OST-mediated breast cancer (BrCa) inhibition were still largely unknown. In this study, we made full use of the GSE85871 dataset to identify potential targets of OST in BrCa via multiple bioinformatics analysis. Next, a series of in vitro experiments were conducted to check the role of GNG7 in BrCa and the relationship between OST and GNG7. Through a series of bioinformatics analyses, GNG7 was identified as a potential target of OST, which could be significant upregulated by OST exposure in BrCa cells. Besides, GNG7 was lowly expressed in BrCa tissues compared with normal breast tissues, and BrCa patients with low GNG7 expression had shorter overall survival (OS) and relapse-free survival (RFS) compared with those with high GNG7 expression. Moreover, GNG7 silencing significantly enhanced cell proliferation and inhibited apoptosis, and exogenous overexpression of GNG7 showed reverse effects on BrCa cells. Last but not least, GNG7 inhibition could notably rescue OST-mediated cytotoxic effects. In summary, we identified GNG7 as a novel target for OST in BrCa and a potential tumor suppressor. Thus, OST could be therapeutically beneficial for BrCa through a GNG7-dependent mechanism.
研究领域:
癌症生物学
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分子生物学
·
药理学
·
生物信息学
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产品关联
DOI
PubMed
高影响因子
Antibiotics-Basel
·
2022
·Vol. 11
·期 11
·1660
·
IF 5.222
Yan Wang,Lanyu Liu,Qiping Wu,Qiufen Yin,Feifan Xie
Background: The pharmacokinetic/pharmacodynamic (PK/PD) index of carbapenems that best correlates with in vivo antimicrobial activity is percent time of dosing interval in which free drug concentration remains above MIC (%fT > MIC), while the magnitudes of the PK/PD index of carbapenems remains undefined in critically ill sepsis patients.Methods: A sepsis rat model was first developed by comparing the survival outcomes after intraperitoneal injection of different inoculum size (1–10 × 107CFU) ofPseudomonas aeruginosaATCC9027 (MIC = 0.125 mg/L) in neutropenic rats. The PK characteristics of the model drug meropenem in the developed sepsis rat model was then evaluated, and PK modeling and simulation was applied to design meropenem dosing regimens attaining various PD targets (40%fT > MIC, 100%fT > MIC, and 100%fT > 4 × MIC). The microbiological response and survival outcomes for different meropenem treatment regimens were investigated in the rat sepsis model (n= 12 for each group).Results: The optimal inoculum for the rat sepsis model was 1 × 107CFU ofPseudomonas aeruginosaATCC9027. A one-compartment model with first-order absorption best described the PK of meropenem in sepsis rats. Pronounced survival prolongation and lower hazard risk were observed in the treatment groups of 50 or 75 mg/kg/q2.4h (100%fT > MIC) and 75 mg/kg/q2h (100%fT > 4 × MIC) compared to the 75 mg/kg/q6h (40%fT > MIC) group, while meropenem groups with PD targets of 100%fT > MIC and 100%fT > 4 × MIC showed comparable survival curves. Microbiological response for different PD targets is inconclusive due to irregular bacterial counts in blood samples.Conclusions: The PD target of 40%fT > MIC is suboptimal for sepsis rats, and the aggressive 100%fT > 4 × MIC target does not provide a survival benefit against the target of 100%fT > MIC.Keywords:pharmacokinetics;PK/PD;survival;meropenem;sepsis rat model
研究领域:
药理学
·
感染病学
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产品关联
DOI
PubMed
高影响因子
Bioengineering & Translational Medicine
·
2021
·Vol. 7
·期 2
·e10290
·
IF 10.711
Fabiao Hu,Changping Deng,Yiwen Zhou,Yuping Liu,Tong Zhang,Peiwen Zhang,Zhangting Zhao,Hui Miao,Wenyun Zheng,Wenliang Zhang,Meiyan Wang,Xingyuan Ma
Regulation of the apoptotic pathway plays a critical role in inducing tumor cell death and circumventing drug resistance. Survivin protein is the strongest inhibitor of apoptosis found so far. It is highly expressed in several cancers and is a promising target for cancer therapy. However, clinical applications are limited by incomplete inhibition of survivin expression. Here, we present a novel strategy that extended the release of YM155 (an effective survivin inhibitor that works by inhibiting the activity of survivin promoter) and TATm-survivin (T34A) (TmSm) protein (survivin protein mutant with penetrating peptide, a potential anticancer protein therapeutic) via tumor matrix microenvironment-mediated ferritin heavy chain nanocages (FTH1 NCs), enabling significant inhibition of survivin activity at both transcript and protein levels. FTS (FTH1-matrix metalloproteinase-2-TmSm)/YM155 NC synthesis was easily scaled up, and these NCs could sequentially release TmSm protein through matrix metalloproteinase-2 and promote YM155 to enter the nucleus via transferrin receptor 1 (TfR1) binding, which increased the cytotoxicity and apoptosis of Capan-2 and A549 cells compared to that with individual drugs. Moreover, FTS/YM155 NCs enhanced drug accumulation at tumor sites and had a higher tumor inhibition rate (88.86%) than the compounds alone in A549 tumor-bearing mice. In addition, FTS/YM155 NCs exerted significant survivin downregulation (4.43-fold) and caspase-3 upregulation (4.31-fold) and showed better therapeutic outcomes without inducing organ injury, which highlights their promising future clinical application in precision therapy. This tumor microenvironment-responsive platform could be harnessed to develop an effective therapy via multilevel inhibition of cancer targets.
研究领域:
癌症生物学
·
纳米技术
·
药学
·
分子生物学
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治疗学
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产品关联
DOI
PubMed
高影响因子
Dose-Response
·
2019
·
IF 2.451
Yiying Gao,Xugang Li,Jingjing Gao,Zhen Zhang,Yang Feng,Jihua Nie,Wei Zhu,Shuyu Zhang,Jianping Cao
Radiation-induced lung injury is a major dose-limiting toxicity that occurs due to thoracic radiotherapy. Metabolomics is a powerful quantitative measurement of low-molecular-weight metabolites in response to environmental disturbances. However, the metabolomic profiles of radiation-induced lung injury have not been reported yet. In this study, male Sprague-Dawley rats were subjected to a single dose of 10 or 20 Gy irradiation to the right lung. One week after radiation, the obvious morphological alteration of lung tissues after radiation was observed by hematoxylin and eosin staining through a transmission electron microscope. We then analyzed the metabolites and related pathways of radiation-induced lung injury by gas chromatography–mass spectrometry, and a total of 453 metabolites were identified. Compared to the nonirradiated left lung, 19 metabolites (8 upregulated and 11 downregulated) showed a significant difference in 10 Gy irradiated lung tissues, including mucic acid, methyl-β-d-galactopyranoside, quinoline-4-carboxylic acid, and pyridoxine. There were 31 differential metabolites (16 upregulated and 15 downregulated) between 20 Gy irradiated and nonirradiated lung tissues, including taurine, piperine, 1,2,4-benzenetriol, and lactamide. The Kyoto Encyclopedia of Genes and Genomes–based pathway analysis enriched 32 metabolic pathways between the irradiated and nonirradiated lung tissues, including pyrimidine metabolism, ATP-binding cassette transporters, aminoacyl-tRNA biosynthesis, and β-alanine metabolism. Among the dysregulated metabolites, we found that taurine promoted clonogenic survival and reduced radiation-induced necrosis in human embryonic lung fibroblast (HELF) cells. This study provides evidence indicating that radiation induces metabolic alterations of the lung. These findings significantly advance our understanding of the pathophysiology of radiation-induced lung injury from the perspective of metabolism.
研究领域:
医学
·
放射生物学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）