Agronomy-Basel
·
2022
·Vol. 12
·期 7
·1489
·
IF 3.949
Kai Yao,Yanqing Wang,Yanyou Wu
Little is known about the competition between and niche differentiation of water and nutrients between angiosperm and gymnosperm tree species under prolonged drought stress, especially in fragile environments. We imposed 21 d drought and competition treatments onBroussonetia papyriferaandPlatycladus orientalisand measured water, N, and P contents, the isotopic composition of N and C, the activity of P assimilation enzymes, and stomatal conductance under solo planting and mixed planting to characterize resource diversity and competition in response to treatments. The N content, δ13C, δ15N, phosphomonoesterase, phosphodiesterase,gs, and foliage water content were significantly affected by the soil water content. The δ15N content in young leaves showed that N competition between these two plants could be alleviated through niche differentiation, but the changes in the PDE: PME ratio for these two plants indicated that they lost the niche differentiation of the P source under drought stress. Additionally, it was observed that foliage water content, WUE, N contents, and N and P sources were significantly affected by interspecific competition, andBroussonetia papyriferabenefited from water competition under moderate drought. Our results indicate that plants have different competition and niche differentiation modes to different nutrients under drought stress, and the effect of interspecific water competition should be seriously considered in mixed forests in semiarid areas.Keywords:angiosperm;gymnosperm;interspecific competition;water stress;stable isotopic
研究领域:
植物学
·
生态学
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DOI
高影响因子
EMBO JOURNAL
·
2020
·Vol. 39
·期 5
·e101679
·
IF 9.889
Jun Wu,Wen-Jia Tian,Yang Liu,Huanhuan J Wang,Jiangli Zheng,Xin Wang,Han Pan,Ji Li,Junyu Luo,Xuerui Yang,Lester F Lau,H Troy Ghashghaei,Qin Shen
Adult neural stem cells (NSCs) reside in specialized niches, which hold a balanced number of NSCs, their progeny, and other cells. How niche capacity is regulated to contain a specific number of NSCs remains unclear. Here, we show that ependyma-derived matricellular protein CCN1 (cellular communication network factor 1) negatively regulates niche capacity and NSC number in the adult ventricular–subventricular zone (V-SVZ). Adult ependyma-specific deletion of Ccn1 transiently enhanced NSC proliferation and reduced neuronal differentiation in mice, increasing the numbers of NSCs and NSC units. Although proliferation of NSCs and neurogenesis seen in Ccn1 knockout mice eventually returned to normal, the expanded NSC pool was maintained in the V-SVZ until old age. Inhibition of EGFR signaling prevented expansion of the NSC population observed in CCN1 deficient mice. Thus, ependyma-derived CCN1 restricts NSC expansion in the adult brain to maintain the proper niche capacity of the V-SVZ.
研究领域:
神经科学
·
干细胞生物学
·
分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yu-Yue Lu,Shu-Lin Li,Fu-Peng Li,Zi-Hao Long,Ting-Ting Lu,Nai-Yong Liu
Three Tomicus bark beetles ( T. yunnanensis , T. brevipilosus and T. minor ) coexist in the trunks of Pinus yunnanensis but possess relatively independent spatio-temporal ecological niches. This makes them become a good case for addressing functional differentiation of chemosensory-related proteins among closely related species in odorant recognition and insecticide sequestering. In this study, we used odorant binding protein (OBP) orthologues highly expressed in antennae to illustrate this question. We first identified 33, 35 and 34 OBPs from T. yunnanensis , T. brevipilosus and T. minor , respectively, with 33 groups of OBP orthologues. Sequence and phylogenetic analyses revealed the conservation and differences of OBP orthologues across three Tomicus beetles. Expression profiles revealed that the majority of Tomicus OBPs were abundantly expressed in antennae where 11 groups of OBP orthologues shared a significantly antenna-enriched distribution. Four groups of antennal OBP orthologues (OBP2, OBP9, OBP10 and OBP16 totaling 12 genes) presented diverse ligand-binding properties, responding differently to five classes of ecologically relevant odorants as well as various insecticides. In particular, OBP orthologues were tuned differentially to host and non-host odorants, and chlorpyrifos was identified as the best ligand for 12 Tomicus OBPs. Our study thus sheds light on functional conservation and divergence of OBP orthologues among three sympatric Tomicus bark beetles, possibly as an implication for the early or late of host colonization, ecological niche differences and the strong habitat adaptation.
研究领域:
昆虫学
·
分子生物学
·
化学生态学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Mingjian Liu,Xiaowen Yu,Mengyao Yang,Wangxinze Shu,Furong Cao,Qian Liu,Jun Wang,Yong Jiang
Nanoplastic is increasing in environments and can address toxic effects on various organisms. Particle size, concentration, and surface functionalization most influence nanoplastic toxicity. Besides, nanoplastic can adsorb other contaminants (e.g., antibiotics) to aggravate its adverse effects. The combined effects of nanoplastics and antibiotics on planktonic/benthic microbial communities , however, are still largely unknown. In this study, the combined effects of polystyrene nanoplastic and ofloxacin on the structure, assembly, and metabolic activities of marine microbial communities were investigated based on amplicon sequencing data. The results mainly demonstrate that: (1) nanoplastic and ofloxacin have greater impacts on prokaryotic communities than eukaryotic ones; (2) niche breadths of planktonic prokaryotes and benthic eukaryotes were shrank with both high nanoplastic and ofloxacin concentrations; (3) increased ofloxacin mainly reduces nodes/edges of co-occurrence networks, while nanoplastic centralizes network modularity; (4) increased nanoplastic under high ofloxacin concentration induces more differential prokaryotic pathways in planktonic communities, while benthic communities are less influenced. The present work indicates that co-presence of nanoplastics and ofloxacin has synergistic combined effects on community structure shifts, niche breadth shrinking, network simplifying, and differential prokaryotic pathways inducing in marine microbial communities, suggesting nanoplastics and its combined impacts with other pollutions should be paid with more concerns.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Lin-Mei Pu,Peng-Fei Wang,Yu-Yue Lu,An-Jin Yang,Li-Li Liu,Nai-Yong Liu
Three sympatric Tomicus bark beetles, Tomicus yunnanensis , Tomicus brevipilosus and Tomicus minor that coexist in the trunks of a pine but occupy different spatiotemporal ecological niches, are the best-studied examples for addressing functional differentiation of chemosensory protein (CSP) orthologs. Here, totally 36 CSP-coding genes belonging to 12 groups of orthologs were identified from three Tomicus beetles. Tomicus CSP orthologs shared the high conservation and slight differences in sequence characteristics, phylogenetic relationships and tissue expression profiles. Binding assays revealed that 12 antenna-enriched Tomicus CSPs could respond strongly to non-host volatiles, pheromones and insecticides where each group of CSP orthologs presented diverse ligand-binding properties. CSP1 and CSP2 could interact strongly with benzaldehyde and salicylaldehyde (dissociation constant, K i < 14 μM) whereas CSP10 and CSP11 preferred to bind 1-hexanol and acetophenone (K i < 13 μM). All the proteins were tuned to chlorpyrifos with particularly high affinities (K i < 10 μM). Two conserved residues (glutamine, Q64 and leucine, L72) of CSP11 orthologs contributed to the specific binding to optimal ligands, but exhibited opposite binding properties between chlorpyrifos and other four compounds. Our study sheds light on the functional conservation and divergence of Tomicus CSP orthologs in olfactory reception and insecticide resistance, implying the correlation between ecological niche differentiation and olfactory specialization in three Tomicus bar beetles.
研究领域:
昆虫学
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分子生物学
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化学生态学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2021
·
IF 15.881
Anran Yuan,Fan Xia,Qiong Bian,Haibin Wu,Yueting Gu,Tao Wang,Ruxuan Wang,Lingling Huang,Qiaoling Huang,Yuefeng Rao,Daishun Ling,Fangyuan Li,Jianqing Gao
Androgenetic alopecia (AGA) is highly prevalent in current society but lacks effective treatments. The dysregulation of the hair follicle niche induced by excessive reactive oxygen species (ROS) and insufficient vascularization in the perifollicular microenvironment is the leading cause of AGA. Herein, we designed a ceria nanozyme (CeNZ)-integrated microneedles patch (Ce-MNs) that can alleviate oxidative stress and promote angiogenesis simultaneously to reshape the perifollicular microenvironment for AGA treatment. On the basis of the excellent mechanical strength of Ce-MNs, the encapsulated CeNZs with catalase- and superoxide-mimic activities can be efficiently delivered into skin to scavenge excessive ROS. Moreover, the mechanical stimulation induced by the administration of MNs can remodel the microvasculature in the balding region. Compared with minoxidil, a widely used clinical drug for AGA treatment, Ce-MNs exhibited accelerated hair regeneration in the AGA mouse model at a lower administration frequency without inducing significant skin damage. Consequently, such a safe and perifollicular microenvironment-shaping MNs patch shows great potential for clinical AGA treatment.
研究领域:
纳米技术
·
皮肤科
·
药学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Xing Zhao,Liya Wang,Ya-Jun Fu,Fei Yu,Kai Li,Yu-Qiang Wang,Yingqiang Guo,Shaobing Zhou,Wei Yang
Intestinal stem cells (ISCs) engage in proliferation to maintain a stable stem cell population and differentiate into functional epithelial subpopulations. This intricate process is upheld by various signals derived from the host and gut microbiota, establishing an ISC niche. However, during inflammatory bowel disease (IBD), this signaling niche undergoes dramatic changes, leading to impaired ISC and hindered restoration of the damaged intestinal epithelial barrier. This study introduces intestinal inflammatory microenvironment-responsive microsphere vehicles designed to remodel the ISC niche, offering an approach to treat IBD. Using an advanced emulsion technique, these microsphere vehicles specifically target colonic inflammation sites, delivering a responsive release of MXene and l-arginine. This delivery system is formulated to modulate intestinal flora and immune responses effectively. l-arginine is converted into nitric oxide to regulate the gut microbiome, while MXene serves as a nanoimmunomodulator to stabilize immune homeostasis. Our findings demonstrate that the anti-inflammatory properties of the microspheres are key to promoting epithelial repair and remodeling of the ISC niche. This study highlights the role of antioxidant microspheres as anti-inflammatory agents that indirectly support ISC function and gut regeneration.
研究领域:
药理学
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生物医学工程
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免疫学
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微生物学
·
干细胞研究
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Xuejun Wang,Hao Wu,Chuhan Dai,Xiaoyu Wang,Lvjing Wang,Jianming Xu,Zhenmei Lu
Co-contamination of organic pollutants and heavy metals is universal in the natural environment. Dibutyl phthalate (DBP), a typical plasticizer , frequently coexists with cadmium (Cd) in nature. However, little attention has been given to the impacts of co-contamination by DBP and Cd on microbial communities or the responses of microbes. To address this, a microcosm experiment was conducted by supplying the exogenous DBP-degrading bacterium Glutamicibacter nicotianae ZM05 to investigate the interplay among DBP-Cd co-contamination, the exogenous DBP-degrading bacterium G. nicotianae ZM05, and indigenous microorganisms . To adapt to co-contamination stress, microbial communities adjust their diversity, interactions, and functions. The stability of the microbial community decreased under co-contamination, as evidenced by lower diversity, simpler network, and fewer ecological niches . Microbial interactions were strengthened, as evidenced by enriched pathways related to microbial communications. Meanwhile, interactions between microorganisms enhanced the environmental fitness of the exogenous DBP-degrading bacterium ZM05. Based on co-occurrence network prediction and coculture experiments, metabolic interactions between the non-DBP-degrading bacterium Cupriavidus metallidurans ZM16 and ZM05 were proven. Strain ZM16 utilized protocatechuic acid, a DBP downstream metabolite, to relieve acid inhibition and adsorbed Cd to relieve toxic stress. These findings help to explain the responses of bacterial and fungal communities to DBP-Cd co-contamination and provide new insights for the construction of degrading consortia for bioremediation.
研究领域:
环境微生物学
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生物修复
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生态毒理学
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微生物生态学
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产品关联
DOI
PubMed
高影响因子
Cell Reports
·
2023
·
IF 8.8
Dan Zhong,Haowei Jiang,Chengzhuo Zhou,Abrar Ahmed,Hongji Li,Xiaona Wei,Qiuyu Lian,Melodi Tastemel,Hongyi Xin,Mei Ge,Chenhong Zhang,Lili Jing
The commensal microbiota regulates the self-renewal and differentiation of hematopoietic stem and progenitor cells (HSPCs) in bone marrow. Whether and how the microbiota influences HSPC development during embryogenesis is unclear. Using gnotobiotic zebrafish, we show that the microbiota is necessary for HSPC development and differentiation. Individual bacterial strains differentially affect HSPC formation, independent of their effects on myeloid cells. Early-life dysbiosis in chd8 -/- zebrafish impairs HSPC development. Wild-type microbiota promote HSPC development by controlling basal inflammatory cytokine expression in kidney niche, and chd8 -/- commensals elicit elevated inflammatory cytokines that reduce HSPCs and enhance myeloid differentiation. We identify an Aeromonas veronii strain with immuno-modulatory activities that fails to induce HSPC development in wild-type fish but selectively inhibits kidney cytokine expression and rebalances HSPC development in chd8 -/- zebrafish. Our studies highlight the important roles of a balanced microbiome during early HSPC development that ensure proper establishment of lineal precursor for adult hematopoietic system.
研究领域:
免疫学
·
血液学
·
微生物学
·
发育生物学
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DOI
PubMed
高影响因子
Mucosal Immunology
·
2019
·Vol. 12
·期 4
·945-957
·
IF 7.352
Qiu Miao,Huang Keqing,Liu Yanzhuo,Yang Yuqing,Tang Honglin,Liu Xiaoxiao,Wang Chenlong,Chen Honglei,Xiong Yu,Zhang Jing,Yang Jing
High-fat diet (HFD) promotes lung pre-metastatic niche formation and metastasis. Thus, there is an urgent need to identify the underlying mechanisms and develop strategies to overcome them. Here we demonstrate that glycyrrhizic acid (GA) prevents HFD-enhanced pre-metastatic niche formation and metastasis through gut microbiota. GA reduced HFD-enhanced myeloid-derived suppressor cell recruitment, pro-metastatic protein S100A8/A9 expression and metastasis burden of 4T1 breast cancer and B16F10 melanoma, accompanied by gut microbiota alteration and colonic macrophage polarization far away the M1-like phenotype. These parameters were greatly decreased by treatment with antibiotics, recolonization of Desulfovibrio vulgaris and Clostridium sordellii , and administration of lipopolysaccharide or deoxycholic acid. Macrophage depletion attenuated HFD-enhanced pre-metastatic niche formation and metastasis, but failed to further affect the effects of GA. Mechanistically, counteraction of HFD-enhanced gut microbiota dysbiosis by GA inhibited Gr-1 + myeloid cell migration and S100A8/A9 expression through decreasing the proportion of M1-like macrophages and their production of CCL2 and TNF-α in the colons via LPS/HMGB1/NF-κB signaling inactivation. Together, targeting the gut microbiota by GA to modulate colonic macrophages could be a novel strategy for the prevention of HFD-enhanced pre-metastatic niche formation and metastasis.
研究领域:
肿瘤学
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微生物学
·
免疫学
·
营养学
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2025
·Vol. 11
·期 9
·
IF 12.5
Kai Tang,Yufan Zheng,Guanshuo Hu,Ying Xin,Keming Li,Cunyu Zhang,Xi Chen,Bai Zhang,Xueyi Li,Bing Hu,Qiong Jia,Yong-ping Zheng,Mo Yang,Youhua Tan
Tumor cells with organ-specific metastasis traits arise in primary lesions with substantial variations of local niche mechanics owing to intratumoral heterogeneity. However, the roles of mechanically heterogeneous primary tumor microenvironment in metastatic organotropism remain an enigma. This study reports that persistent priming in soft but not stiff niches that mimic primary tumor mechanical heterogeneity induces transcriptional reprogramming reminiscent of neuron and promotes the acquisition of brain metastatic potential. Soft-primed cells generate brain metastases in vivo through enhanced transendothelial migration across blood-brain barrier and brain colonization, which is further supported by the findings that tumor cells residing in local soft niches of primary xenografts exhibit brain metastatic tropism. Mechanistically, soft niches suppress cytoskeleton-nucleus–mediated mechanotransduction, which promotes histone deacetylase 3 activity. Inhibiting histone deacetylase 3 abolishes niche softness-induced brain metastatic ability. Collectively, this study uncovers a previously unappreciated role of local niche softness within primary tumors in brain metastasis, highlighting the significance of primary tumor mechanical heterogeneity in metastatic organotropism.
研究领域:
肿瘤学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Qingyun Ma,Qingqing Li,Jie Wang,Rebecca E. Parales,Lin Li,Zhiyong Ruan
Herbicide mixtures are a new and effective agricultural strategy for managing suppress weed resistance and have been widely used in controlling weeding growth in maize fields. However, the potential ecotoxicological impact of these mixtures on the microbial community structure and function within various root-associated niches, remains inadequately understood. Here, the effects of nicosulfuron , mesotrione and atrazine on soil enzyme activity and microbial community structure and function were investigated when applied alone and in combination. The findings indicated that herbicide mixtures exhibit a prolonged half-life compared to single herbicides. Ecological niches are the major factor influencing the structure and functions of the microbial community, with the rhizosphere exhibiting a more intensive response to herbicide stress. Herbicides significantly inhibited the activities of soil functional enzymes, including dehydrogenase , urease and sucrose in the short-term. Single herbicide did not drastically influence the alpha or beta diversity of the soil bacterial community, but herbicide mixtures significantly increased the richness of the fungal community. Meanwhile, the key functional microbial populations, such as Pseudomonas and Enterobacteriaceae, were significantly altered by herbicide stress. Both individual and combined use of the three herbicides reduced the complexity and stability of the bacterial network but increased the interspecific cooperations of fungal community in the rhizosphere. Moreover, by quantification of residual herbicide concentrations in the soil, we showed that the degradation period of the herbicide mixture was longer than that of single herbicides. Herbicide mixtures increased the contents of NO 3 − -N and NH 4 + -N in the soil in the short-term. Overall, our study provided a comprehensive insight into the response of maize root-associated microbial communities to herbicide mixtures and facilitated the assessment of the ecological risks posed by herbicide mixtures to the agricultural environment from an agricultural sustainability perspective.
研究领域:
农业科学
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生态学
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微生物学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）