ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Ke Gao,Kai Hua,Xi Chen,Congyi Zheng,Xingtong Li,Qingyan Wu,Lingrui Ji,Linxiao Wang,Wei Wei,Liping Lu
Phthalates (PAEs) constitute the primary components of indoor dust pollutants and significantly impact human reproductive health. However, research on novel alternatives to PAEs and the risk assessment of mixed exposure has remained relatively sparse. In this study, 193 indoor dust samples were collected in 2022 from various campus locations, including classrooms, canteens, dormitories, offices, and laboratories. Forty-four traditional PAEs and their alternatives were identified, with concentrations ranging from 0.44 to 91.5 μg/g. Di(2-ethylhexyl) phthalate (DEHP) and dioctyl terephthalate (DEHTH) were the predominant compounds, with mean concentrations of 86.3 and 59.2 μg/g, respectively. The ingestion pathway was the principal route of exposure, with dormitories identified as the primary exposure sites. The mixed reproductive toxicity equivalent factor (TEFmix) of PAEs and their alternatives was developed using a quantitative structure–activity relationship (QSAR) model in conjunction with machine learning algorithms. The TEFmix was found to be lower than the sum of individual PAEs, potentially due to the antagonistic effects of PAE monomers on reproductive health. Under high-exposure scenarios, the TEFmix of PAEs in canteen dust was determined to be 0.245, surpassing values observed in other environments. Females exhibited a higher risk, with dormitories presenting a greater exposure risk than those in other indoor locations. This study provided essential data to inform regulatory measures aimed at mitigating the impact of PAEs and their alternatives in indoor dust on human reproductive health.
研究领域:
环境科学
·
毒理学
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Na Liu,Xiaowei Jin,Junying Zhou,Yeyao Wang,Qi Yang,Fengchang Wu,John P. Giesy,Andrew C. Johnson
Triadimefon, a broad-spectrum, systemic fungicide used to protect agricultural crops is popular in China. However, sub-lethal effects of triadimefon on aquatic organisms remained poorly understood, and its risks to aquatic organisms were unclear. In the current study, thresholds for chronic toxicity to five aquatic organisms were determined and a PNEC based on reproductive fitness of nine aquatic organisms was derived through use of a species sensitivity distribution (SSD). NOECs, based on reproduction or inhabitation of growth, for Oryzias latipes , Daphnia magna , Brachionus calyciflorus , Heterocypris incongruens and Soirodela polyrhiza were 5, 25, 80, 320 and 500 μg L −1 , respectively, and the final PNEC derived was 3.66 μg L −1 . A screening-level hazard assessment of surface water based on both measured environment concentrations (ND∼5.22 μg L −1 ) in 3 lakes, 2 reservoirs and 1 river and predicted environment concentrations (0.36–65 μg L −1 ) in a simulated river and pond, identified unacceptable hazard to aquatic organisms posed by triadimefon, with maximum hazard quotients (HQs) of 1.43 and 17.8, respectively. Potential deleterious effects and hazards or risks of exposure of aquatic organisms from current patterns of use of triadimefon in surface water if of concern. Since HQs were relatively small and the benefits large, it is suggested that mitigations be applied to allow use while minimizing potential for adverse effects on aquatic organisms.
研究领域:
生态毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Mei-Ling Le,Yi-Qi Wan,Jia-Yu Xue,Shi-Yi Deng,Bin-Hui Wang,Li-Hai Wu,Hou-Peng Wang,Yang-Yang Yuan,Xiao-Dong Kuang,Tao Luo,Da-Lei Zhang
Hexafluoropropylene oxide (HFPO) homologues (HFPOs), presenting HFPO-dimeric acid (DA), HFPO-trimeric acid (TA) and HFPO-tetrameric acid (TeA), are emerging replacements for legacy perfluorooctanoic acid that are increasingly used for a variety of industrial applications. These novel fluorinated compounds have attracted much attention due to their environmental ubiquity and potential toxicities. However, there is a lack of clarity about their toxicological impact on male reproductive health. In our study, the mouse model was used to investigate the toxic effects of HFPOs on male reproductive function and their underlying mechanisms. The results revealed that male mice exposed to HFPOs for 8 weeks presented structural and functional abnormalities in the testis and mature sperm, as evidenced by destructed seminiferous tubule, decreased testosterone levels and sperm quality. Furthermore, treatment with HFPOs elevated reactive oxygen species and malondialdehyde content and diminished superoxide dismutase activity and succinate dehydrogenase levels, along with downregulated anti-apoptotic BCL-2 expression and upregulated pro-apoptotic BAX expression. Moreover, oral administration of HFPOs impaired testicular mitochondrial structure along with reduction in ATP content and mitochondrial DNA copy number and disruption of mitochondrial dynamics. In summary, HFPOs can impair male fertility by disrupting spermatogenesis and reducing sperm quality through multiple mechanisms, including mitochondrial dysfunction-induced oxidative stress, apoptosis, and suppression of steroidogenesis. This work aims to improve our comprehension regarding the potential risks and adverse impacts of exposure to HFPO congeners on male reproductive health.
研究领域:
毒理学
·
生殖生物学
·
环境健康科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
REPRODUCTIVE TOXICOLOGY
·
2024
·
IF 3.3
Luyao Yang,Shuangshuang Wang,Jing Jin,Jiahui Wang,Wenyue Chen,Yun Xue,Liang Sheng,Yongning Zhai,Weifeng Yao
Sucralose, the extensively utilized sweetener, might lead to metabolic disorders with prolonged consumption, but it remains uncertain if sucralose has any impact on female reproductive health. We incorporated sucralose into drinking water and observed food intake, body weight, estrous cycle, follicular development, serum hormones, and insulin sensitivity of mice. The mice did not experience any changes in their food intake or body weight after consuming sucralose. However, they displayed irregularities in the estrous cycle, marked by a reduced count of primordial, primary, and secondary follicles, coupled with a significant increase in the number of antral follicles. There was a decline in follicle-stimulating hormone (FSH), estradiol (E2), and progesterone (P4) levels, while testosterone (T) and luteinizing hormone (LH) levels surged, leading to a notable elevation in the LH / FSH ratio. Sucralose also induced insulin resistance, as evidenced by elevated insulin levels and impaired insulin tolerance, which responded to an increase in bacterial-derived serum endotoxin. By eliminating insulin resistance with rosiglitazone (RSG), eradicating intestinal flora-derived endotoxins with neomycin (NEO), or enhancing intestinal barrier function with indole-3-carbinol (I3C), the abnormalities in estrous cycle, disruptions in follicular development, hormonal imbalances and elevation in serum endotoxins induced by sucralose were successfully reversed. The present study indicates that sucralose-induced follicular dysplasia in mice is probably related to impaired intestinal permeability, infiltration of endotoxins, initiation of systemic inflammation, and insulin resistance.
研究领域:
生殖生物学
·
内分泌学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Qigang Fan,Yi Sun,Qingjun Shen,Yuxin Wang,Jisheng Nie,Yanbing Lin,Shuaishuai Xing,Yan Li,Benhong Xu,Jianjun Liu,Xiaoting Shen,Guangbo Qu,Jun Xie,Liqin Zeng,Huidong Zhang
Li exposure is becoming a new prominent major issue in energy-environment-health field. However, its reproductive health risk has been less explored. In this study, we find that exposure to 28-fold REED ( r eally e nvironmental e xposure d ose) of Li is enough to induce mouse miscarriage by altering the structures and functions of placenta. Exposure to ≥ 5.6-fold REED of Li is enough to alter the structure and functions of ovary and placenta, and exposure to ≥ 28-fold REED of Li alters fetus morphology and uterus functions, showing a susceptibility to Li exposure as the order ovary > placenta > fetus > uterus. Some characteristics (such as AMH (anti-müllerian hormone) levels in serum, the mRNA levels of Pgr (progesterone receptor) and Nr2f2 in placenta, and the mRNA levels of Cyp19A1 and Sohlh2 in Ovary) are significantly altered with exposure to 5.6-fold REED of Li, which could be used as sensitive targets to predict reproductive health risk with Li exposure. Moreover, the doses of Li in clinical Li treatment (28- or 56-fold REED of Li) should be more cautious, especially for women. Collectively, this study identifies Li exposure as a new risk factor for female reproductive healthy in energy-environment-health field, which deserves the critical attention by governments, enterprises, and hospitals.
研究领域:
环境科学
·
毒理学
·
生殖生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Advanced Research
·
2024
·
IF 11.4
Pei-Yu Dong,Shuai Yuan,Yu-Mei Chen Yan,Yu Chen,Yue Bai,Yang Dong,Yin-Yin Li,Wei Shen,Xi-Feng Zhang
Iron deficiency is a common nutritional issue that seriously affects male reproductive health. Lotus root polysaccharide iron (LRPF), a novel nutritional supplement, may ameliorate the damage caused by iron deficiency, however, the mechanism is unclear. In this study, we comprehensively determined the benefits of LRPF on reproduction in iron-deficient mice by integrating transcriptomics, microbiomics and serum metabolomics. Microbiomics showed that LRPF could restore changes to the intestinal microbiota caused by iron deficiency. Metabolomics results showed that LRPF stabilised steroid hormone and fatty acid metabolism in iron-deficient mice, reduced the content of ethyl chrysanthemumate (EC) and ameliorated the reproductive impairment. The transcriptomic analysis showed that LRPF regulated steroid hormone synthesis and the peroxisome proliferator-activated receptor (PPAR) signalling pathway in iron-deficient mice. In vitro experiments showed that LRPF could promote steroid hormone synthesis in Leydig cells by activating PPARγ. In conclusion, this study highlights the advantage of LRPF to improve testicular development.
研究领域:
营养学
·
生殖生物学
·
分子生物学
·
转录组学
·
代谢组学
·
微生物组学
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
·
2023
·
IF 3.5
Hao Lin,Shaowei Wang,Yunbing Tang,Zhiyan Hu,Xiaofang Chen,Huitao Li,Yang Zhu,Yiyan Wang,Yi Liu,Ren-shan Ge
Benzophenones (BPs) are a class of chemicals found in various personal care and cosmetic products, such as sunscreens and lotions. Their usage is known to cause reproductive and hormonal health risks, but the exact mechanism of action remains unknown. In this study, we investigated the effects of BPs on human and rat placental 3β-hydroxysteroid dehydrogenases (3β-HSDs), which play a crucial role in the biosynthesis of steroid hormones , particularly progesterone . We tested inhibitory effects of 12 BPs, and performed structure-activity relationship (SAR) and in silico docking analysis. The potency of BPs to inhibit human 3β-HSD1 (h3β-HSD1) is BP-1 (IC 50 , 8.37 μM)>BP-2 (9.06 μM)>BP-12 (94.24 μM)>BP-7 (1160 μM) >BP-8 (1257 μM) >BP-6 (1410 μM) > other BPs (ineffective at 100 μM). The potency of BPs on rat r3β-HSD4 is BP-1 (IC 50 , 4.31 μM)>BP-2 (117.3 μM)>BP-6 (669 μM) >BP-3 (820 μM)>other BPs (ineffective at 100 μM). BP-1, BP-2, and BP-12 are mixed h3β-HSD1 inhibitors and BP-1 is a mixed r3β-HSD4 inhibitor. LogP, lowest binding energy, and molecular weight were positively associated with IC 50 for h3β-HSD1, while LogS was negatively associated with IC 50 . The 4-OH substitution in the benzene ring plays a key role in enhancing the effectiveness of inhibiting h3β-HSD1 and r3β-HSD4, possibly through increasing water solubility and decreasing lipophilicity by forming hydrogen bonds . BP-1 and BP-2 inhibited progesterone production in human JAr cells. Docking analysis shows that 2-OH of BP-1 forms hydrogen bonds with catalytic residue Ser125 of h3β-HSD1 and Thr125 of r3β-HSD4. In conclusion, this study demonstrates that BP-1 and BP-2 are moderate inhibitors of h3β-HSD1 and BP-1 is a moderate inhibitor of r3β-HSD4. There is a significant SAR differences for 3β-HSD homologues between BPs and distinct species-dependent inhibition of placental 3β-HSDs.
研究领域:
毒理学
·
药理学
·
内分泌学
·
分子生物学
·
计算化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Xinghua Yu,Rui Yao,Ge Meng,Lingan Zeng,Leyi Zhou,Yingkang Shen,Amali Upekshika Wijayaraja,Reshmi Hareendra Kariyapperuma,Wentao Xu,Yuxiang Mei,Xinyan Gu,Wen-Long Lei,Pengfei Zhang,Fei Sun
Given the extensive use of plastic materials in modern society, there is an escalating concern about the potential risks associated with exposure to plastic products. This study investigated the impact of plastic leachates from boiled-water-treated cups, including polypropylene (PP), high-density polyethylene (HDPE), low-density polyethylene (LDPE), polystyrene (PS), and polyethylene terephthalate (PET), on male reproductive health. Experimental mice were administered daily doses of the plastic leachates for 180 consecutive days. Histological analysis of the testes and epididymis was conducted, revealing vacuolization and absence of sperms in the seminiferous tubules of mice treated with PP, HDPE, LDPE, and PS, while PET exhibited lower reproductive toxicity. Furthermore, transcriptomic and metabolomic profiling were employed to identify key genes and metabolites related to plastic exposure. Transcriptome analysis showed significant changes in genes associated with spermatogenesis following exposure to leachates, while metabolome analysis indicated an impact on the lipid metabolism pathway. Overall, the study provides evidence that oral exposure to leachates from boiled-water-treated plastic cups could negatively affect spermatogenesis and lipid metabolism, thereby posing risks to male reproductive health. These findings offer crucial insights into the potential risks associated with plastic consumption and may advocate for the selection of relatively safe plastic cups for everyday use.
研究领域:
毒理学
·
生殖生物学
·
环境健康科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
iMeta
·
2025
·
IF 33.2
Pan Li,Li Xie,Huimin Zheng,Yinglin Feng,Feihong Mai,Wenli Tang,Jiajia Wang,Zixin Lan,Shuaijun Lv,Thisun Jayawardana,Sabrina Koentgen,Shuangbin Xu,Zhengwei Wan,Yunjie Chen,Haiyan Xu,Sj Shen,Fan Zhang,Yuanhao Yang,Georgina Hold,Fangjie He,Emad M. El-Omar,Guangchuang Yu,Xia Chen
Polycystic ovary syndrome (PCOS) is a prevalent endocrine and reproductive disorder affecting women of reproductive age. While the gut microbiota has been implicated in PCOS pathophysiology, the role of microbial-derived metabolites as mediators of host–microbe interactions remains poorly defined. Here, we integrated untargeted gut metabolomics with metagenomic profiling in patients with PCOS and identified a marked depletion of 3,4-dihydroxyphenylacetic acid (DHPAA), a flavonoid-derived microbial catabolite. Oral administration of DHPAA ameliorated PCOS-like phenotypes in two mouse models by suppressing bone morphogenetic protein signaling and reducing anti-Müllerian hormone (AMH) levels. We found that DHPAA production depends on gut microbial degradation of dietary flavonoids. We further identified a bacterial species, Streptococcus thermophilus , consistently depleted in PCOS across two human cohorts and a mouse model, restored DHPAA levels and improved reproductive outcomes in mice. Conversely, a β-galactosidase-deficient mutant of S. thermophilus failed to confer these benefits, highlighting β-galactosidase as a critical enzyme in DHPAA biosynthesis. Our findings establish DHPAA as a key microbial metabolite linking diet, microbiota, and reproductive health, and propose its potential as a novel therapeutic candidate for PCOS. Graphical By integrating multi-level and multi-omics analyses, we identify 3,4-dihydroxyphenylacetic acid (DHPAA), a gut microbiota-derived degradation product of dietary flavonoids, as a key bioactive end-product responsible for the beneficial effects against polycystic ovary syndrome (PCOS). DHPAA exerts direct therapeutic effects on PCOS-like phenotypes independent of the gut microbiota through inhibition of bone morphogenetic protein signaling. We further demonstrate that Streptococcus thermophilus metabolizes flavonoids into DHPAA via β-galactosidase activity, thereby contributing to the improvement of PCOS symptoms. These findings highlight the essential role of the gut microbiota in transforming flavonoids into functionally active metabolites and establish DHPAA as a critical mediator linking microbial metabolism to host reproductive health. This study deepens our understanding of the microbiota-metabolite-host axis in PCOS pathophysiology and provides a promising candidate molecule for therapeutic development. Highlights 3,4-dihydroxyphenylacetic acid (DHPAA) is identified as a key microbial-derived metabolite responsible for the protective effects of flavonoids against polycystic ovary syndrome (PCOS). Streptococcus thermophilus produces DHPAA from flavonoids through β-galactosidase-mediated metabolism. Gut microbiota is essential for transforming flavonoids into bioactive metabolites that confer protection against PCOS.
研究领域:
微生物组研究
·
内分泌学
·
生殖医学
·
代谢疾病
·
营养科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 5
·e202201190
·
IF 2.9
Busra Dincer,Ahmet Bulent Yazici,Irfan Cinar,Erdem Toktay,Jale Selli,Elif Cadirci,Zafer Bayraktutan,Esra Yazici
The pathophysiological mechanism behind the link between antipsychotic drugs and sexual dysfunction is still unknown. The goal of this research is to compare the potential effects of antipsychotics on the male reproductive system. Fifty rats were randomly assigned into the five groups indicated: Control, Haloperidol, Risperidone, Quetiapine and Aripiprazole. Sperm parameters were significantly impaired in all antipsychotics-treated groups. Haloperidol and Risperidone significantly decreased the level of testosterone. All antipsychotics had significantly reduced inhibin B level. A significant reduction was observed in SOD activity in all antipsychotics-treated groups. While GSH levels diminished, MDA levels were rising in the Haloperidol and Risperidone groups. Also, the GSH level was significantly elevated in the Quetiapine and Aripiprazole groups. By causing oxidative stress and altering hormone levels, Haloperidol and Risperidone are damaging to male reproductivity. This study represents useful starting point for exploring further aspects of the underlying mechanisms reproductive toxicity of antipsychotics. Graphical
研究领域:
药理学
·
毒理学
·
生殖健康
·
氧化应激研究
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qi Zhao,Zhencheng Chen,Chen-Wei Shan,Tao Zhan,Chen-Yang Han,Guo-Cheng Han,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
Human chorionic gonadotropin (HCG), a vital protein for pregnancy determination and a marker for trophoblastic diseases , finds application in monitoring early pregnancy and ectopic pregnancy . This study presents an innovative approach employing electrochemical immunosensors for enhanced HCG detection, utilizing Anti-HCG antibodies and gold nanoparticles (AuNPs) in the sensor platform. Two sensor configurations were optimized: BSA/Anti-HCG/c-AuNPs/MEL/e-AuNPs/SPCE with [Fe(CN) 6 ] 3−/4− as a redox probe (1) and BSA/Anti-HCG/PPy/e-AuNPs/SPCE using polypyrrole (PPy) as a redox probe (2). The first sensor offers linear correlation in the 0.10–500.00 pg∙mL −1 HCG range, with a limit of detection (LOD) of 0.06 pg∙mL −1 , sensitivity of 32.25 μA∙pg −1 ∙mL∙cm −2 , RSD <2.47 %, and a recovery rate of 101.03–104.81 %. The second sensor widens the HCG detection range (40.00 fg∙mL −1 -5.00 pg∙mL −1 ) with a LOD of 16.53 fg∙mL −1 , ensuring precision (RSD <1.04 %) and a recovery range of 94.61–106.07 % in serum samples. These electrochemical immunosensors have transformative potential in biomarker detection, offering enhanced sensitivity, selectivity , and stability for advanced healthcare diagnostics.
研究领域:
生物医学工程
·
分析化学
·
生殖健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 16
·8764
·
IF 4.9
Jun Ma,Ruixue Huang,Huai Zhang,Dongju Liu,Xiaodong Dong,Yan Xiong,Xianrong Xiong,Daoliang Lan,Wei Fu,Honghong He,Jian Li,Shi Yin
Fumonisin B1 (FB1), a mycotoxin produced byFusariumspecies, is prevalent in crops and animal feed, posing significant health risks to livestock and humans. FB1 induces oxidative stress in Sertoli cells, destroys testicular structure, and affects spermatogenesis. However, methods to mitigate the reproductive toxicity of FB1 in testes remain unknown. Quercetin, a natural flavonoid antioxidant, may offer protective benefits. This study investigated the protective effects and mechanisms of quercetin against FB1-induced reproductive toxicity in TM4 cells (a Sertoli cell line). The results indicated that 40 μM quercetin improved cell viability, reduced apoptosis, and preserved cell functions. Quercetin also decreased reactive oxygen species (ROS) levels in TM4 cells exposed to FB1, enhanced the expression of antioxidant genes, and improved mitochondrial membrane potential. Compared with FB1 alone, the combination of quercetin and FB1 increased ATP levels, as well as pyruvate and lactic acid, the key glycolysis products. Furthermore, this combination elevated the mRNA and protein expression of glycolysis-related genes, including glucose-6-phosphate isomerase 1 (Gpi1), hexokinase 2 (Hk2), aldolase (Aldoa), pyruvate kinase, muscle (Pkm), lactate dehydrogenase A (Ldha) and phosphofructokinase, liver, B-type (Pfkl). Quercetin also boosted the activity of PKM and LDHA, two crucial glycolytic enzymes. In summary, quercetin mitigates FB1-induced toxicity in TM4 cells by reducing ROS levels and enhancing glycolysis. This study offers new insights into preventing and treating FB1-induced toxic damage to the male reproductive system and highlights the potential application of quercetin.
研究领域:
毒理学
·
细胞生物学
·
生殖健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）