Toxins
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2024
·Vol. 16
·期 3
·121
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IF 3.9
Hira Sayed,Qiongqiong Zhang,Yu Tang,Yanan Wang,Yongpeng Guo,Jianyun Zhang,Cheng Ji,Qiugang Ma,Lihong Zhao
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin found in many agricultural products and can cause reproductive disorders, mainly affecting spermatogenesis in male animals. Rutin (RUT) is a natural flavonoid compound recognized for its significant antioxidant, anti-inflammatory and estrogenic properties. The present study aimed to determine the protective role of RUT against ZEN-induced reproductive toxicity in male mice. Twenty-four adult Kunming male mice were divided into four groups: control, RUT (500 mg/kg RUT), ZEN (10 mg/kg ZEN), ZEN + RUT (500 mg/kg RUT + 10 mg/kg ZEN), with six replicates per treatment. The results indicated that RUT mitigated ZEN-induced disruption in spermatogenic cell arrangement, decreased spermatozoa count, and increased sperm mortality in the testes. RUT significantly restored ZEN-induced reduction in T, FSH, LH, and E2 serum levels. Moreover, RUT mitigated ZEN-induced apoptosis by increasing the mRNA expression level ofbcl-2, decreasing the mRNA expression level ofkiss1-r, and decreasing the protein expression level of caspase 8 in reproductive tissues. These findings indicate the protective role of RUT against ZEN-induced reproductive toxicity in male mice by regulating gonadotropin and testosterone secretions to maintain normal spermatogenesis via the HPG axis, which may provide a new application direction for RUT as a therapeutic agent to mitigate ZEN-induced reproductive toxicity.
研究领域:
毒理学
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生殖生物学
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分子生物学
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DOI
PubMed
高影响因子
iScience
·
2023
·
IF 5.8
Fuwen Liu,Xue Cao,Lei Zhou
Nonylphenol (NP), a widely recognized endocrine disruptor, exhibits lipophobic properties that drive its accumulation in adipose tissue, leading to various physiological disruptions. Using Caenorhabditis elegans , this study investigated the effects of NP exposure on lipid homeostasis and physiological indicators. NP exposure increased lipid storage, hindered reproduction and growth, and altered phospholipid composition. Transcriptional analysis revealed NP’s promotion of lipogenesis and inhibition of lipolysis. Metabolites related to lipid metabolism like citrate, amino acids, and neurotransmitters, along with lipids, collectively influenced physiological processes. This work elucidates the complex link between lipid metabolism disturbances and NP-induced physiological disruptions, enhancing our understanding of NP’s multifaceted toxicity.
研究领域:
环境毒理学
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分子生物学
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DOI
高影响因子
Forests
·
2024
·Vol. 15
·期 7
·1157
·
IF 2.4
Huihui Liu,Lin Chen,Qinghua Ma,Xinghong Wang,Sufang Zhang,Xuguo Zhou
Hox genes, specifically the bithorax complex (ubx,abdominal-a, andabdominal-b), play a crucial role in specifying posterior abdominal development and serve as key regulators of germline gene development in insects. However, the function of the bithorax complex in the pine caterpillar moth,Dendrolimus punctatus, a major pine tree defoliator in China, remains largely unknown. Specifically,Abdominal-A(Abd-a) controls regional variation in abdominal segmentation in model insects such asDrosophilaandTribolium; however, its role inD. Punctatusremains unexplored. In this study, CRISPR/Cas9 was used to functionally characterizeAbd-ainD. punctatus. Two target sites were selected, and the genotypes and phenotypes of the G0 and G1 generations were evaluated. Our findings indicate that knocking outAbd-aled to an abnormality in the posterior segments A2–A7, as well as the loss of appendages, mainly prolegs, and affected the thoracic T3 segmentation as well as wing development. Moreover, mutation inAbd-aalso impacted anal and reproductive development. Taken together, these results demonstrate thatDpAbd-ais essential for embryonic and reproductive development inD. punctatusand could be a promising target for genetic control of this devastating conifer defoliator.
研究领域:
昆虫学
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遗传学
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分子生物学
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DOI
高影响因子
Ecohydrology
·
2024
·
IF 2.5
Yuqing Lin,Ting Li,Qiuwen Chen,Ruxia Qiao,Shufeng He,Tao Feng,Jun Wang
The establishment of reservoirs, which alter water temperature and flow dynamics, significantly affects the indigenous fish population, yet the underlying molecular mechanisms remain unclear. To investigate effects of the changing water temperature and flow rate on fish ovaries, we subjected female Coreius guichenoti to the combination of different water temperatures (17°C, 20°C and 23°C denoted as L, N and H, respectively) and flow velocities (0 and 0.5 m/s denoted as S and V, respectively). After 30-day experiment period, we identified 393, 545, 300 and 657 differentially expressed transcripts in HV versus NV, LV versus NV, HS versus NV and LS versus NV, respectively. The transcriptome results showed that both environmental factors could significantly influence oogenesis, with water temperature exerting a more pronounced effect than flow velocity. In particular, the higher temperature (HV and HS) led to a tendency towards masculinization and even infertility in females, which were consistent with the histopathology results. By contrast, the lower temperature (LV and LS) promoted the progression of desirable female attributes, where static water conditions (LS) had a greater effect compared with flowing water (LV). These findings were of great significance for the adaptive operation of reservoirs to create reasonable and precise ecological flows for managing fish reproduction.
研究领域:
生态学
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分子生物学
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鱼类学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Yufei Xie,Qiuyu Liu,Wenting Lin,Yuan Ren,Lingtian Xie
Various anabolic androgenic steroids (AAS) are frequently detected in aquatic environments, yet the potential ecotoxicological impacts of their increased load have not received adequate attention. This study investigates the effects of exposure to the androgen nandrolone on sex differentiation, gonadal development, and the expression of hormones, enzymes, and proteins related to the hypothalamic-pituitary-gonadal (HPG) axis in zebrafish. The results indicate that exposure to nandrolone at 60 days post-fertilization promotes the maturation of spermatogonia, inhibits the development of oocytes, and induces their apoptosis. Additionally, there were dose-dependent changes in male differentiation and hormone levels within the HPG axis: in the high-dose group (5 μg/L), males constituted 73 % of the population, and levels of E2 hormones in gonadal tissues decreased by 33 %, while T hormone levels increased by 55 %. Furthermore, the expression of genes essential for ovarian differentiation were downregulated, whereas the expression of testicular differentiation-related genes were upregulated. The molecular docking results show that the drug can form hydrogen bonds with key proteins, which have high affinity, and are more likely to cause conformational changes in the proteins, thereby altering their activity. KEGG analysis suggests that the MAPK signaling pathway is likely the target pathway through which nandrolone exposure induces reproductive toxicity.
研究领域:
生态毒理学
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分子生物学
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内分泌学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Yufan Nie,Simin Yu,Luyao Zhang,Zikang Wang,Rui Liu,Yuping Liu,Wentao Zhu,Zhiqiang Zhou,Jinling Diao
Declines in reptile populations due to climate warming and environmental pollution have been documented. Although recent ecotoxicological studies of reptiles have increased, little is known about how these two stressors interact to affect reptile reproductive processes. This study investigated the single and combined effects of atrazine and warming on reproduction in female lizards (Eremias argus) following 10 weeks of exposure to environmentally relevant concentrations of atrazine (0–10 mg·kg–1) at two temperature treatments (control or warming). Reproductive traits, clutch characteristics, and endpoints related to endocrine disruption (HPG axis gene expression) and energy metabolism (enzyme activity, hepatic metabolomics) were assessed. Atrazine inhibits female reproduction by disrupting HPG axis-related gene expression and energy metabolism, resulting in delayed spawning time and reduced fecundity. In contrast, warming promoted female reproduction and partly alleviated the inhibitory effects of atrazine, which is related to the upregulation of HPG axis-related gene expression and an additional energy metabolism compensatory response. Additionally, atrazine and/or warming altered the direction and intensity of the trade-off between egg number and size and affected maternal nutritional investment in eggs. These findings highlight the complex interplay of environmental stressors on lizard reproduction and add to a better understanding of reptile reproductive strategies and ecological consequences under environmental stress.
研究领域:
生态学
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毒理学
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内分泌学
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产品关联
DOI
PubMed
高影响因子
Journal of Hazardous Materials Advances
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2025
·
IF 7.7
Peigen Chen,Lina Chen,Xing Yang,Tingting Li,Cong Fang
The pervasive contamination by Di(2-ethylhexyl) phthalate (DEHP) and nanoplastics (NPs) poses a significant threat to reproductive health, yet the combined effects of these pollutants on endometrial receptivity remain poorly understood. Our study investigates the impact of chronic DEHP and NPs co-exposure on endometrial ciliary function in mice and explores the potential protective role of dietary inulin. Utilizing a multifaceted approach—including transcriptomic analysis, immunohistochemistry, scanning electron microscopy, and immunofluorescence—we reveal that DEHP and NPs synergistically impair endometrial cilia, crucial for embryo implantation. Remarkably, inulin supplementation restores ciliary structure and function, mitigating the detrimental effects of these pollutants. Our findings suggest that dietary interventions could offer a practical strategy to counteract the reproductive toxicity of environmental contaminants, providing new insights into maintaining reproductive health in polluted environments. This research underscores the urgency of addressing environmental pollutants and highlights the potential of inulin as a protective agent against reproductive toxicity.
研究领域:
生殖生物学
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环境医学
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营养学
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DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2019
·
IF 2.697
Xianliang Yi,Tongtong Chi,Bei Liu,Chenxi Liu,Guanqun Feng,Xinyu Dai,Keke Zhang,Hao Zhou
Given the absorbability of nZnO and its inevitable coexistence with trace metals such as Cd and Pb in coastal environment, nZnO can adsorb these pollutants thereby affecting their distribution in different media of marine ecosystem. The marine copepod Tigriopus japonicus was applied in the present study to investigate the combined effect of nZnO and Cd or Pb on mortality and reproduction in marine organisms. For acute exposure, presence of 1.0 mg/L nZnO increased the toxicity of both Cd and Pb, as their LC 50 decreased from 5.9 and 75.4 mg/L to 3.95 and 48.0 mg/L, respectively. For 21 d chronic exposure, the reproduction of the copepod was influenced by Cd and Pb at environmental relevant concentrations, and the interaction between nZnO and Cd or Pb appeared to be antagonistic. The waterborne Cd and Pb concentration was affected by nZnO for neither acute nor chronic exposure, indicating no adsorption of these two metals to nZnO at relative low concentration. The overall findings of this study suggested the binary exposure to nZnO/Cd or nZnO/Pb might have potential different toxic mechanisms between acute and chronic exposure.
研究领域:
环境科学
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毒理学
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海洋生物学
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DOI
PubMed
高影响因子
Cell Journal
·
2021
·
IF 2.479
Sadaf Mahboudi,Kazem Parivar,Zohreh Mazaheri,Shiva Irani
Objective Numerous evidence indicates that microRNAs (miRNAs) are critical regulators in the spermatogenesisprocess. The aim of this study was to investigate Mir-106b cluster regulates primordial germ cells (PGCs) differentiationfrom human mesenchymal stem cells (MSCs). Materials and Methods In this experimental study, samples containing male adipose (n: 9 samples- age: 25-40 years)were obtained from cosmetic surgeries performed for the liposuction in Imam Khomeini Hospital. The differentiationof MSCs into PGCs was accomplished by transfection of a lentivector expressing miR-106b . The transfection of miR-106b was also confirmed by the detection of a clear green fluorescent protein (GFP) signal in MSCs. MSCs weretreated with bone morphogenic factor 4 (BMP4) protein, as a putative inducer of PGCs differentiation, to induce thedifferentiation of MSCs into PGCs (positive control). After 4 days of transfection, the expression of miR-106b , STELLA,and FRAGILIS genes was evaluated by real-time polymerase chain reaction (PCR). Also, the levels of thymocytedifferentiation antigen 1 (Thy1) protein was assessed by the western blot analysis. The cell surface expression of CD90was also determined by immunocytochemistry method. The cytotoxicity of miR-106b was examined in MSCs after 24,48, and 72 hours using the MTT assay. Results MSCs treated with BMP4 or transfected by miR-106b were successfully differentiated into PGCs. The resultsof this study also showed that the expression of miR-106b was significantly increased after 48 hours from transfection.Also, we showed STELLA, FARGILIS , as well as the protein expression of Thy1, was significantly higher in MSCstransfected by lentivector expressing miR-106b in comparison with MSCs treated with BMP4 (P≤0.05). MTT assayshowed miR-106b was no toxic during 72 hours in 1 µg/ml dose, that this amount could elevated germ cells markersignificantly higher than other experimental groups (P≤0.05). Conclusion According to this findings, it appears that miR-106b plays an essential role in the differentiation of MSCsinto PGCs.
研究领域:
分子生物学
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生殖医学
·
干细胞研究
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DOI
PubMed
高影响因子
Animals
·
2025
·Vol. 15
·期 2
·140
·
IF 2.7
Kexin Ma,Yueyao Yang,Yifan Li,Chuan Li,Taicheng Li,Haiyan Ma,Zibin Jiang,He Zhou,Wei Wang
Simple SummaryOur study investigates the karyotype of a vital polyploid fish,Carassius auratus gibelio. We found 150 chromosomes with 6 nucleolar organizer regions, suggesting complex chromosomal interactions. Male and femaleC. gibelioshow different chromosome pairings, indicating asynchronous evolution. The formation of two trivalents or two bivalents and two univalents from six NOR bearing chromosomes in maleC. gibeliois considered evidence of being in the process of rediploidization. The presence of only univalents and bivalents in the oocytes of femaleC. gibelioindicates that their cytogenetics are more stable, suggesting that they have completed rediploidization. Our findings contribute to the understanding of the underlying mechanisms and evolutionary outcomes of this species’ genome. They provide relevant insights for further exploration of the reproductive modes of polyploid organisms and breeding strategies.Carassius auratus gibelio, a significant polyploid fish in Chinese aquaculture, can reproduce through both unisexual gynogenesis and sexual reproduction. WhileC. gibeliois known to exhibit polyploidy throughout its lifecycle, whether the homologous pairing of chromosomes is associated with the mode of reproduction remains unclear. Our study determined its karyotype as 2n = 150, comprising 42 metacentric, 24 submetacentric, 6 subtelocentric, and 78 telocentric chromosomes. Using AgNORs, CMA3/DAPI double fluorescence staining, and FISH, we identified six nucleolar organizer regions inC. gibeliochromosomes. FemaleC. gibelioexhibited only univalent and bivalent configurations, while males showed a range of chromosome configurations from univalent to hexavalent. CMA3-positive sites and FISH signals in trivalents spermatocytes hint at homologous triploidization. The formation of two trivalents or two bivalents and two univalents from six NORs bearing chromosomes in maleC. gibeliois considered evidence of being in the process of rediploidization. The presence of only univalents and bivalents in the oocytes of femaleC. gibelioindicates that their cytogenetics are more stable, suggesting that they have completed rediploidization. Asynchronous progression of polyploidization may lead to differences in reproductive patterns between male and femaleC. gibelio. Our results offer cytogenetic insights into the polyploidy and evolution ofC. gibelio, providing foundational cytogenomics data. This study provides relevant references for revealing the distinct reproductive strategies of male and femaleC. gibelio: under stable conditions, gynogenesis enables rapid clonal reproduction and swift population growth, while sexual reproduction enhances the genetic diversity within the population.
研究领域:
细胞遗传学
·
进化生物学
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DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Zhihua Ni,Xinling Tian,Wenbo Zhao,Wenkai Hu,Jinghua Lv,Xiaoli Sun,Yajie Zhang,Yiwen Zhang,Yuming Zhang,Baoku Li,Fengsong Liu
Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL. Here, the effects of ORL on Daphnia magna were evaluated using a 21-day chronic test at concentrations of 1, 10, 100, and 1000 μg/L. We found the inhibition of feeding and swimming activities in the 100 and 1000 μg/L ORL exposed D. magna , respectively. Their digestive enzyme activities and metabolites were reduced even at 1 μg/L ORL exposure. It is noteworthy that exposure to 100 μg/L ORL induced a decrease in the reproductive capacity of D. magna , although no discernible genotoxicity was observed. To identify the toxicological mechanisms of ORL, a metabolic analysis was conducted on D. magna exposed to 1000 μg/L ORL. A comprehensive reduction in carbohydrates, lipids, and amino acids was observed, which resulted in a blockage of metabolic flux towards the TCA cycle, as evidenced by mitochondrial dysfunction. These findings substantiate the detrimental impact of ORL on D. magna and provide insights into the underlying molecular mechanisms from a metabolic perspective.
研究领域:
环境科学
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毒理学
·
生态学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Ying Ding,Jin-Ting Zheng,Ya-Ning Wang,Di Wu,Dong Zhu
There is growing interest in interactions of microplastics (MPs) with other pollutants. However, there is limited understanding of the combined effects of MPs and silver nanoparticles (AgNPs) on nontarget soil organisms. This work aimed to examine the effects of exposure to various AgNPs' concentrations alone (0, 0.1, 1, 10, 100, 1000 mg kg −1 , 50 nm) and in combination with polyvinyl chloride microplastics (PVC MPs, 80−250 μm) at 0.1% concentration for 28 days on reproduction, Ag accumulation, C/N ratio, and isotopic fractionation of the standard soil fauna collembolan Folsomia candida . Results showed that compared to the AgNPs exposure alone, the presence of MPs significantly reduced reproduction by 51.4% and markedly increased Ag content in collembolans by 87.7% at 1000 mg kg −1 AgNPs, which evidenced a synergistic effect. Co-exposure to MPs and AgNPs resulted in a noticeable reduction in the C/N ratio in F. candida body tissues by 9.90% and 5.27% at 1 and 10 mg kg −1 AgNPs, respectively, showing additive and synergistic effects. Additionally, this co-exposure altered stable isotope fractionation, with the highest increments of δ 15 N by 32.3% and inhibition of δ 13 C by 2.62%, demonstrating the turnover of nutrients shift in the collembolan tissues. Collectively, this study demonstrates that con-current exposure to environmentally relevant concentration of MPs and relatively high doses of AgNPs synergistically induces toxic effects on F. candida , leading to Ag accumulation and reproduction decline. These findings imply that MPs could alter collembolans' responses to AgNPs exposure, potentially enhancing the metal ions’ bioavailability in soil environments and posing ecotoxicological threats to soil-dwelling organisms.
研究领域:
环境科学
·
生态毒理学
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产品关联
DOI
PubMed
高影响因子
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