BIOELECTROCHEMISTRY
·
2020
·
IF 4.722
Jiasheng Wang,Dongwei Wang,Ni Hui
The application of polypeptides in bio-interfaces and biosensors is of great interest because polypeptides are biocompatible and easy to design. A novel polymer nanocomposite was prepared by the electropolymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) with a newly designed polypeptide. The nanocomposite polypeptide doped PEDOT (PEDOT/PEP), with a 3D microporous network structure, large surface area and excellent antifouling ability, was utilized for the attachment of BRCA1 complementary oligonucleotides to construct a DNA biosensor. The fabricated DNA biosensor showed favorable selectivity (with a detection limit of 0.0034 pM) and high sensitivity. The biosensor was also capable of detecting the target DNA (BRCA1) in 1% (V/V) human serum samples. The combination of a conducting polymer PEDOT with an antifouling and biocompatible polypeptide demonstrates a new method for preparing electrochemical sensors, that are capable of detecting targets in complex biological samples without strong nonspecific protein adsorption.
研究领域:
生物传感器
·
材料科学
·
癌症研究
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2019
·
IF 2.032
Dan Wang,Yanan Zheng,Xiaosu Fan,Lina Xu,Ting Pang,Ting Liu,Legui Liang,Shan Huang,Qi Xiao
Mercury pollution has always been a huge threat to human health due to its significant toxicity. Thus, it's the continuing goal to obtain new mercury detection techniques that are cost-effective, operational stable, performance efficient, and applicable to the environmental and biological milieus. In this research, the soluble pigment pyocyanin with anti-bacterial and anti-fungal activities, the biosynthesis pathway of which was engineered under the regulation of Hg 2+ -dependent transcriptional activator MerR, was firstly used as the visual detection signal in the whole-cell biosensor. The engineered biosensor displayed optical sensing window and a good linearity for Hg 2+ in the range of 25–1000 nM, and the detection limit could reach as low as 10 nM. It permitted on-site detection of bioavailable Hg 2+ with extraordinary selectivity and could resist the interferences of extra metal ions. What's more, the developed biosensor performed function well in a wide pH range (pH 4–10) as well as the environmental water. By fully imitating and utilizing the biosystems from nature, the engineered colorimetric biosensor has great economic and performance advantages over most chemosensors as well as whole-cell biosensors in the practical application of detecting Hg 2+ in the contaminated aquatic systems.
研究领域:
环境科学
·
微生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Wei Xie,Shu Jia,Rongjun Yu,Jianjiang Xue,Shulian Bai,Chenxing Wang,Gang Tian,Qiongyuan Zhang,Xiao Gui,Lilin Wang,Silin Chen,Ke Lin,Wei Mao,Yu Huang,Jiangling Wu,Gang Liu
Sepsis represents a life-threatening systemic inflammatory condition characterized by organ dysfunction, where the identification of specific biomarkers and development of effective diagnostic modalities are critical for timely clinical intervention to mitigate disease progression. Emerging evidence positions exosomal microRNAs (exomiRs) as promising novel biomarkers with multi-dimensional clinical utility spanning early diagnosis, therapeutic efficacy assessment, and longitudinal prognostic monitoring in sepsis management. However, the direct rapid detection of exomiRs in clinical specimens undergoes severe critical challenges due to the absence of robust detection methodologies with these characteristics of operational simplicity, high sensitivity and analytical robustness. Here, we designed and constructed a hairpin/loop-constrained three-dimensional topological self-assembling nanostructure, which was denoted as DNA molecular lock (DML), and developed a spatially allosteric-mediated electrochemical nanobiosensor for ultrasensitive and specific analysis of exomiR-125b. This independent, rapid, and portable detection method, combined with Ti 3 C 2 T x MXene/AuAgNPs nanozymes, achieved excellent sensitivity with a linear range from 2 fM to 200 nM and a low detection limit of 0.15 fM. It provided required low-volume plasma samples (2 mL) for analysis while supported Bluetooth-enabled wireless communication and real-time data processing. Furthermore, integration of machine learning algorithms with this developed nanobiosensor improved differential diagnostic accuracy to 96.6 % in the training cohort ( n = 83) and 89.2 % in the validation cohort ( n = 57), effectively distinguishing sepsis patients (SPs), general inflammation patients (GIPs), and healthy donors (HDs). Our study establishes the sensor's efficacy in improving sepsis detection through biomarker-guided analysis. These findings highlight the potential application of this developed nanobiosensors for portable, personalized bedside monitoring of exomiRs as the point-of-care testing (POCT) platforms.
研究领域:
生物传感器
·
分子诊断学
·
纳米技术
·
微小RNA研究
·
临床检验医学
·
医疗健康中的机器学习
✓ 已验证
产品关联
DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2020
·Vol. 12
·期 9
·1079-1086
·
IF 1.128
Hao Haitao
At present, chemical biosensors still have poor reproducibility and stability due to that the density of nanomaterials particles is not ideal. Therefore, an optimal design method of electrochemical biosensor based on gold nanomaterials is proposed. Two types of sensors, namely DNA biosensor
and immune biosensor, were designed in this work. A DNA biosensor with gold nanorod nanocomposite modified electrode was prepared, and its electrical activity was measured by Methylene blue. In contrast, an electrochemical immune sensor with gold nanorod-modified electrode was prepared and
used for detection of alpha fetoprotein. The experimental results show that the designed DNA biosensor and electrochemical immune sensor have the advantages of simple preparation, high sensitivity, good selectivity and good stability.
研究领域:
生物传感技术
·
纳米生物学
·
医学检测
✓ 已验证
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Qiao Liu,Xing-Li Xie,Chang-Jie Mao,Jing-Shuai Chen,He-Lin Niu,Ji-Ming Song
A highly selective DNA-based electrochemiluminescence (ECL) based biosensor is described for the detection of human IgG. It is exploiting the effect of steric hindrance that affects the strength of the ECL signal in the presence of IgG. Digoxin-linked signaling DNA was specifically bound to IgG, and this causes steric hindrance which limits the ability of DNA to hybridize with capturing DNA attached to a gold electrode. Europium (II) doped CdSe quantum dots were covalently linked to the DNA in order to generate the ECL signal. Using this steric hindrance hybridization method, the ECL signal of the biosensor were proportional to the concentration of IgG with a wide linear range and a 14 pM detection limit. Conceivably, the method can be expanded to the detection of a wide range of proteins for which homologous recognition elements are available.
研究领域:
生物化学
·
分析化学
·
生物传感器
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Jia Li,Junlin He,Chengli Zhang,Jun Chen,Weiran Mao,Chao Yu
In this study, a dual-type responsive electrochemical biosensor was developed for the quantitative detection of α2,6-sialylated glycans (α2,6-sial-Gs), a potential biomarker of tumors. The gold nanorods (AuNRs), which exhibited great specific surface area, as well as good biocompatibility , was synthesized by the way of seed growth method. Furthermore, a biotin-streptavidin (biotin-SA) system was introduced to improve the immunoreaction efficiency. Accordingly, a label-free biosensor was fabricated based on AuNRs-SA for the quick detection of α2,6-sial-Gs by recording the signal of differential pulse voltammetry (DPV). Furthermore, to expand the ultrasensitive detection of α2,6-sial-Gs, a carboxylated single-walled carbon nanohorns/sulfur-doped platinum nanocluster (c-SWCNHs/S-PtNC) was synthesized for the first time as a novel signal label, which showed an excellent catalytic performance. The usage of c-SWCNHs/S-PtNC could significantly amplify the electrochemical signal recorded by the amperometric i-t curve. Herein, a sandwich type biosensor was constructed by combining the AuNRs-SA on the electrode and c-SWCNHs/S-PtNC (signal amplifier). The label-free biosensor possessed a linear range from 5 ng mL −1 to 5 μg mL −1 with a detection limit of 0.50 ng mL −1 , and the sandwich-type biosensor possessed a wide linear range from 1 fg mL −1 to 100 ng mL −1 with a detection limit of 0.69 fg mL −1 . Furthermore, the biosensor exhibited excellent recovery and stability, indicating its potential for use in actual samples.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sensing and Bio-Sensing Research
·
2025
·
IF 4.9
Baolin Li,Yaxin Huang,Zixin Zhu,Min Zhong,Guangrong Li,Jinbo Liu
Adenosine triphosphate (ATP) plays a critical role in cellular energy metabolism, acting as the energy source for many biological reactions. Abnormal fluctuations in ATP levels are directly linked to various diseases such as malignancies, bacterial infections, and cardiovascular disorders, making early-stage disease detection through rapid, highly sensitive ATP measurement essential. The current methods for ATP detection suffer from drawbacks such as complex procedures, reliance on expensive instruments, and prolonged reaction times. To this end, we have developed a novel biosensor named ATP Output Sensor Activated by CRISPR (AOSAC).This biosensor employs aptamer-based specific recognition combined with a dual-enzyme, amplification-free system involving Exo III and CRISPR-Cas12a for ATP detection. This sensor operates through an aptamer in a closed duplex structure, upon binding ATP, releases a trigger strand. This strand displaces an activation chain, which in turn interacts with Cas12a's crRNA, initiating trans-cleavage activity that cleaves a single-stranded DNA probe to produce a fluorescent signal. Concurrently, the activation chain's release sites are targeted by Exo III, enhancing signal amplification without the need for complex nucleic acid amplification, thus providing a highly selective, rapid detection method. This technique offers a linear detection range from 0 nM to 20 μM with a detection limit of 44.2 nM, presenting a novel strategy for signal amplification and high specificity in distinguishing ATP from its analogues.
研究领域:
分子生物学
·
生物化学
·
医学检测
✓ 已验证
产品关联
DOI
高影响因子
SENSORS
·
2017
·Vol. 17
·期 6
·1235
·
IF 2.677
Yao Zhao,Xueyong Wei,Niancai Peng,Jiuhong Wang,Zhuangde Jiang
Uric acid (UA) is a kind of purine metabolism product and important in clinical diagnosis. In this work, we present a study of ZnS nanostructures-based electrochemical and photoelectrochemical biosensors for UA detection. Through a simple hydrothermal method and varying the ratio of reaction solvents, we obtained ZnS nanomaterials of one-dimensional to three-dimensional morphologies and they were characterized using field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). To fabricate the UA biosensor and study the effect of material morphology on its performance, ZnS nanomaterials were deposited on indium tin oxide (ITO) conducting glass and then coated with uricase by physical absorption. Three kinds of working electrodes were characterized by cyclic voltammetry method. The effect of material morphology on performance of UA detection was investigated via amperometric response based electrochemical method based on enzymatic reaction. The ZnS urchin-like nanostructures electrode shows better sensitivity compared with those made of nanoparticles and nanoflakes because of its high surface-area-to-volume ratio. The photoelectrochemical method for detection of UA was also studied. The sensitivity was increased 5 times after irradiation of 300 nm UV light. These results indicate that ZnS nanostructures are good candidate materials for developing enzyme-based UA biosensors.Keywords:biosensor;enzyme;nanostructures;ZnS;UA
研究领域:
生物化学
·
材料科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Wenxue Li,Siyuan Chen,Shiwang Xie,Xianmao Lu,Zhou Li,Yujia Lv,Dan Luo
Bacteriorhodopsin is a biological material with excellent photosensitivity properties. It can directly convert optical signals into electrical signals and is widely used in various biosensors. Here, we present a bR-based wearable pH biometer that can be used to monitor wound infection. The mechanism of the pH-sensitive effect of the bR electrode is explained, which generates a transient photovoltage under light irradiation and a negative photovoltage when the lamp is turned off. Since the photoelectric signal of bR is affected by different pH values, the photovoltage is changed by adjusting the pH value. The ratio (Vn/Vp) of negative photovoltage (Vn) to positive photovoltage (Vp) has a good linear relationship (R2 = 0.9911) in the pH range of 4.0–10.0. In vitro experiments using rats as a model confirmed that this wearable pH biometer can monitor pH changes that occur in wound infection.
研究领域:
生物技术
·
生物工程
·
传感器
·
医疗器械
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-9
·
IF 5.3
Si Zhongzhou,Shao Rong,Li Ting,Yang Minghui
Photoelectrochemical biosensor based on Bi 2 S 3 /Bi 2 O 2 CO 3 heterojunction as photoelectrically active material and lactate oxidase was constructed for sensitive detection of lactic acid in serum. Topological transformation method was utilized to prepare Bi 2 S 3 /Bi 2 O 2 CO 3 composites, which can generate electron–hole pairs triggering photoelectrochemical reactions under light illumination. The Bi 2 S 3 /Bi 2 O 2 CO 3 heterojunction effectively promoted the separation and transportation of photogenerated electrons and holes, leading to improved photoelectric conversion efficiency. Lactic acid is catalyzed by lactate oxidase to generate H 2 O 2 , which reacts with photogenerated holes, leading to photocurrent increase, achieving quantitative detection of lactic acid. The prepared biosensor has a wide detection range for lactic acid from 0.01 mM to 10 mM, with detection limit as low as 0.006 mM. In addition, the detection of lactic acid in serum samples further validated the potential application of the sensor. Graphical abstract
研究领域:
生物传感器
·
临床检验
·
纳米材料应用
·
光电化学分析
·
生物医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICAL FIBER TECHNOLOGY
·
2025
·
IF 2.7
Ling Chen,Ruike Liu,Xianfeng Liu,Zhaoyang Li,Guohao Li
A highly economical fiber immunosensor with the harmonic Vernier effect (HVE) is reported for cancer antigen 125 (CA125) tumor marker detection. The sensor architecture is constructed using a special fiber fusion splicer to cascade three sections of single-mode fiber (SMF) in sequence with a large offset. This configuration yields a cascaded Fabry-Perot interferometer (FPI) architecture incorporating the HVE. The misalignment fusion design facilitates direct and seamless interaction between biochemical fluids and optical signals. Experimental results demonstrate that the refractive index sensitivities of the internal envelope and upper envelope reach −5236, and 1163 nm/RIU, respectively, when refractive index increases from 1.334 to 1.339. It has excellent consistency with the simulation results of −5375 nm/RIU and 1257 nm/RIU as refractive index increases from 1.334 to 1.335. Obviously, the refractive index sensitivity is significantly enhanced via the HVE. The designed sensor is further biologically modified with the 50 μg/mL ovarian cancer 125 monoclonal antibody, enabling varying cancer antigen 125 concentrations detection. The proposed biosensor has low detection limit with value of 44.12 fg/mL and a fast response time (<30 min). Therefore, the proposed biosensor has latent application as an ideal platform for disease diagnosis and medical detection.
研究领域:
生物传感器
·
生物光子学
·
癌症诊断
·
光学传感
·
光纤技术
·
生物医学工程
·
免疫传感器
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yihui Ma,Nianzu Liu,Zhenying Xu,Jingjing Wang,Xiliang Luo
The rapid, selective and ultrasensitive assaying of biomarkers remains a challenge relevant to biosensor/diagnostic applications. Herein, a promising electrochemical biosensor based on three-dimensional (3D) nanoporous conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) decorated with gold nanoparticles (AuNPs) was developed, in which methylene blue (MB) was employed as redox indicator for microRNA24 (miRNA24) detection. The unique porous construction was fabricated by a simple hard-template method and cyclic voltammetry (CV) electrochemical technique. AuNPs were electrodeposited onto the porous PEDOT surface to increase the interface conductivity and provide more active sites to load identification elements. The obtained porous biosensor based on conducting polymer PEDOT and AuNPs exhibited significantly enlarged specific surface area (about 2.4 times) and enhanced sensitivity (about 2.1 times), compared to the biosensor based on planar PEDOT. The developed porous biosensor achieved a miRNA24 quantification range from 1 fM to 10 nM with satisfying stability, high selectivity, and a low limit of detection (0.38 fM). The construction of biosensors based on porous conducting polymers decorated with AuNPs offers an effective strategy to increase the electroactive surface area of the sensing interfaces, so as to effectively enhance the sensitivity.
研究领域:
生物传感器
·
电化学
·
纳米技术
·
分析化学
·
分子诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）