ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Chunyu Jiang,Shixing Zhou,Lin Liu,Zokir Toshmatov,Ling Huang,Kai Shi,Chi Zhang,Hua Shao
The chemical profile and the phytotoxicity of Artemisia absinthium essential oil (EO) were investigated to evaluate its potential value as a biopesticide for food safety purposes. A total of 54 compounds were identified in A. absinthium EO, with the most abundant constituents being eucalyptol (25.59%), linalool (11.99%), and β-myrcene (10.05%). The EO, linalool, and a mixture of three major components exhibited potent suppressive activity against four receiver species; however, eucalyptol and β-myrcene showed a much weaker effect. Bioassay-guided fractionation led to the isolation of linalool as the major active compound responsible for the EO’s phytotoxicity. Subsequent scanning electron microscopy (SEM) analysis revealed that linalool significantly inhibited root-hair formation and metaxylem development. This is the first report on the determination of linalool as the major active phytotoxic compound in A. absinthium EO, as well as the elucidation of its mechanism of phytotoxicity from the perspective of root structure changes in the receiver species. Our results suggest that both the EO and its major constituents have potential value as environmentally friendly herbicides.
研究领域:
植物学
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食品科学
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环境科学
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DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-16
·
IF 15.7
Gao Zhou,Rensing Christopher,Wang Jie,Shen Chenhui,Elzaki Mohammed Esmail Abdalla,Li Xiaoyun,Tan Jinfang,Jiang Xiaoqian
RNA interference is an eco-friendly alternative to chemical pesticides, yet its efficacy in lepidopterans like Spodoptera frugiperda ( S. frugiperda ) is limited by poor uptake. Here, we report on ZIF-8 polydopamine nanoparticles that protect dsRNA against enzymatic degradation and active the endocytic/phagosome pathways for increased uptake. Furthermore, the uptake of nano-enabled dsRNA induces the overgrowth of Serratia marcescens , this reduces the S. frugiperda reactive oxygen species (ROS) immune response, increasing the effects of plant’s natural defenses, further inhibiting Enteroccous mundtii growth. This work shows the synergistic potential of nanoparticles for influencing the gut bacteria to prevent resistance mechanisms and for RNAi delivery for pest management.
研究领域:
昆虫学
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分子生物学
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农业科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Yan Wang,Zhengxuan Wang,Yaping Liu,Charles Brennan,Jueting Liu,Jinhong Zhou,Wenbing Zhou,Guiguang Cheng
Natural polysaccharides have been attracted increasing attention in biopesticides for the antiviral effects. Tobacco leaves enriched the polysaccharides and had the potential against tobacco mosaic virus (TMV), however, the chemical structure and anti-TMV effect of polysaccharides from discarded tobacco leaves (DTCP) are still unclear. In this study, an optimized condition for extraction time of 3.5 h, temperature of 90 °C, and solid-liquid ratio of 1:45 led to the maximum extraction value of 21.11 % for DTCP. Subsequently, a homogeneous polysaccharide (DTP) of 3061 Da was purified from DTCP by DEAE and Sephadex G-100 column. The major monosaccharide composition of DTP was glucose (Glc), glucuronic acid (GlcA), galactose (Gal), and galacturonic acid (GalA) in a molar ratio of 3.9:2.6:1.06:0.84. NMR, methylation and IR analysis showed that DTP was a polysaccharide with →4)- β -D-Galp-(1 → 4)- β -D-Galp-(1 → 4)- β -D-Glcp as the main chain, and its branching points were composed of α -D-Glcp-(1→, α -D-GlcpA-(1→ and α -D-GalpA-(1→. Furthermore, DTP exhibited significant inactive and protective effects against TMV with the inhibitory rates of 80.40 % and 76.18 % at 500 μg/mL, respectively. DTP also interfered with the polymerization of TMV-CP to limit its self-assembly. Additionally, DTP could increase the superoxide dismutase, catalase, peroxidase and phenylalanine ammonia lyase activities of tobacco plants by 1.83, 2.73, 3.69 and 4.84 times, respectively, and could alleviate the hypersensitive response and systemic acquired resistance. Hence, DTP could serve as a new plant-derived pesticide for controlling TMV in agriculture.
研究领域:
植物学
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病毒学
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农业科学
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产品关联
DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2018
·Vol. 34
·期 9
·1-10
·
IF 2.1
Peng Huasong,Tan Jian,Bilal Muhammad,Wang Wei,Hu Hongbo,Zhang Xuehong
Phenazine-1-carboxamide (PCN) is one of the major biocontrol agents produced by plant growth-promoting rhizosphere (PGPR) pseudomonads including Pseudomonas chlororaphis . In this study, a combined strategy of genetic modification and statistical experimental designs was applied to obtain mutants of P. chlororaphis strains with high-yield PCN production. To achieve this, the lon gene was knocked out in wild-type P. chlororaphis HT66 and the breeding mutant P3 strain with a non-scar deletion strategy. The resulting HT66Δ lon and P3Δ lon mutants produced a significantly higher PCN production in shake-flask cultures which was 5- and 9-folds greater than their native counterparts. The potential ability of strain P3 Δlon for PCN production was further optimized by statistical designs. A two-level Plackett–Burman (PB) experimental design with six variables was employed to scrutinize medium components that significantly influence PCN production. Notably, glycerol, tryptone, and soy peptone were identified to be the most significant factors ( p < 0.05). Response surface methodology (RSM) based on the central composite design (CCD) was adopted to determine these factors optimal levels and their interactive effects between culture components for PCN production. The predicted maximum PCN production was 9002 mg/L, whereas an actual PCN production of 9174 mg/L was recorded in the validation experiments using the optimal medium containing glycerol 37.08 mL/L, tryptone 20.00 g/L, and soy peptone 25.03 g/L, which was nearly threefolds higher than without optimization and 20-folds higher than the wild-type strain. In conclusion, the results revealed that P. chlororaphis display a high potential for industrial-scale production for phenazine biopesticides.
研究领域:
微生物学
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生物技术
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DOI
PubMed
高影响因子
Micro & Nano Letters
·
2022
·Vol. 17
·期 2
·42-48
·
IF 0.98
Liya Zeng,Zhe Wang
The chemical compositions of Ginkgo biloba exocarp extracts (GBEE) and leaf extracts are similar, however, studies describing the development of GBEE biopesticides are few. In this study, the authors investigated the preparation of GBEE nanoemulsionand and characterized by transmittance, physical stability, droplet size and size distribution for the first time. Insecticidal activity and lipophilicity screening of four different solvents indicated ethyl acetate was satisfactory. Based on dispersibility, centrifugation, heating-cooling cycle, cloud point and long-term stability experiments, two formulations (ethyl acetate/Tween 40-Span 60 (9:1)/water and ethyl acetate/Tween 60-Span 60 (9:1)/water) were optimum to formulate nanoemulsions. However, the smallest droplet size and size distribution (10.93 ± 0.16 nm, PDI = 0.15 ± 0.01) was obtained using the Tween 60/Span 60 combination. The insecticidal activity of GBEE nanoemulsion was evaluated against the third instar larvae of Plutella xylostella . Lethal concentration (LC 50 ) values of the optimized nanoemulsion were 127.38 mg/L at 12 h and 72.80 mg/L at 24 h, respectively. This indicated that GBEE nanoemulsion had higher insecticidal activity than the GBEE. The results proposed that the GBEE nanoemulsion is a potential and effective natural alternative for the management of P. xylostella .
研究领域:
植物保护
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环境科学
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农业科学
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产品关联
DOI
高影响因子
Egyptian Journal of Biological Pest Control
·
2021
·Vol. 31
·期 1
·1-12
·
IF 1.995
Luo Meng,Zhu Dandan,Lin Juntao,Zhou Xinhua,Zheng Changge,Pu Xia
Background Biological pesticides, especially baculovirus, often lose their activity under the influence of external light, temperature, and other changes. This limited the application of them. The present study was aimed to prolong the biological activity and ensure the efficacy of a biological pesticide using microencapsulation technology. Results In this study, gelatin/carboxymethylcellulose (CMC)- Spodoptera litura nucleopolyhedrovirus microcapsules were prepared. The morphological characteristics, apparent morphology, embedding rate, virus loading, particle size, laboratory virulence, and UV resistance of the microencapsulated virus, were tested. The best conditions for preparing gelatin /CMC- S. litura nucleopolyhedrovirus microcapsules include the gelatin/CMC ratio of 9:1, wall material concentration of 1%, core material/wall ration ratio of 1:2, re-condensation pH of 4.67, and curing time of 1 h. The prepared microcapsules of S. litura nucleopolyhedrovirus exhibited a good external appearance and spherical shapes with an average particle size of 13 μm, an embedding rate of 62.53%, and a drug loading of 43.87%. The virulence test showed that the microencapsulated virus lost by 2.21 times of its initial activity than the untreated virus. After being treated with field exposure, the gelatin/CMC shell of the microcapsule can better protect the virus in the wild environment. Conclusion Microencapsulation improves the tolerance of S. litura nuclear polyhedrosis virus to ultraviolet radiation. These results will provide ideas for the research of stable and efficient baculovirus preparations and further promote the application and promotion of environmental friendly biological pesticides.
研究领域:
病毒学
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农业科学
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生物技术
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DOI
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Zhang Shujing,Liu Qi,Han Yunfei,Han Jinghua,Yan Zhiqiang,Wang Yonghong,Zhang Xing
This study was conducted to purify and identify metabolites of antimicrobial activity against phytopathogens from Xenorhabdus nematophila YL001. Three dipeptide compounds were purified from its cell-free cultural broth and identified as (±)-nematophin, cyclo (L-Pro-Gly), and N, N′-dimethyl-cyclo (L-Phe-L-Leu). Nematophin demonstrated a wider antifungal spectrum than the other two compounds. It also exhibited strong inhibitory effects on mycelial growth of Rhizoctonia solani and Phytophthora infestans with EC50 values of 40.00 and 51.25 μg/ml, respectively. Its (S)-configuration structure [(+)-nematophin] was also synthesized and exhibited higher antimicrobial activity than the enantiomeric mixture. The detached leaf assay revealed that nematophin possessed significant preventive and curative efficacy against R. solani on broad bean leaves showing corresponding control efficacies of 93.01 and 94.93% at 1,000 μg/ml, comparable to those of a chemical fungicide (carbendazim) at 500 μg/ml. Additionally, the pot experiments indicated that nematophin could effectively inhibit the disease extension on rice and broad bean plants caused by R. solani. Nematophin also exerted some adverse influences on the sclerotial development of R. solani by dramatically suppressing their formation and maturation at 40.00 μg/ml, as well as their germination at 15.00 μg/ml. Morphological and ultrastructural observations showed that the hyphae of R. solani became twisted, shriveled, and deformed at the growing points after exposure to nematophin at 40.00 μg/ml, and that the subcellular fractions also became abnormal concurrently, especially the mitochondrial structure. These results indicate that nematophin has great potential to be used as a bio-pesticide in agricultural production.
研究领域:
农业科学
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微生物学
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植物病理学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2017
·
IF 1.046
Yunhua Zhang,Jinyun Ji,Xiao Zhao,Ruoheng Jin,Jianhong Li,Hu Wan
A previous study demonstrated that a β- N -acetyl- d -hexosaminidase ( NlHex4 ) from the brown planthopper could serve as a new potential target for biopesticides due to its requirement for molting, and lethal phenotypes were observed via RNA interference . However, the enzymatic properties of NlHex4 remain unclear. In the present study, NlHex4 was expressed, purified and characterized. Recombinant NlHex4 was functionally expressed in baculovirus-infected insect cells and purified with a molecular weight of approximately 70 kDa. Recombinant NlHex4 had an optimal pH and temperature of 6.0 and 60 °C, respectively. Moreover, recombinant NlHex4 exhibited thermal stability at 50 °C for at least 1 h. Furthermore, the activity of recombinant NlHex4 was inhibited by the metal ions Fe 2 + and Fe 3 + and the serine protease inhibitor phenylmethanesulfonyl fluoride. These results reveal the enzymatic properties of NlHex4 and provide a basis for future investigation of the interaction between HEX and chitin oligosaccharide .
研究领域:
昆虫学
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生物化学
·
害虫管理
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产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 1
·173-182
·
IF 4.462
Dongbiao Lü,Yanlu Dong,Zizheng Yan,Xueying Liu,Yongjun Zhang,Daibin Yang,Kanglai He,Zhenying Wang,Ping Wang,Xiangqun Yuan,Yiping Li
BACKGROUND Spodoptera frugiperda is an important invasive agricultural pest that causes huge economic losses worldwide. Gut microorganisms play a vital role in host feeding, digestion, nutrition, immunity, growth and insecticide resistance. Illumina high-throughput sequencing was used to study the gut microbial community dynamics across the life cycle (egg, 1st to 6th instar larvae, pupae, and male and female adults) of S. frugiperda fed on maize leaves. Furthermore, the gut microbial community and food intake of the 5th instar S. frugiperda larvae were studied after feeding them antibiotics. RESULTS Enterobacteriaceae and Enterococcaceae dominated the gut during growth and feeding of the larvae. The relative abundance of Enterobacteriaceae was higher in the 4th and 6th instar larvae. With the increase in larval feeding, the relative abundance of Enterococcaceae gradually increased. In addition, principal coordinate analysis and linear discriminant effect size analysis confirmed differences in the structure of gut microbiota at different developmental stages. After antibiotic treatment, the relative abundance of Firmicutes, Proteobacteria and Fusobacteriota decreased. The relative abundance of Enterococcus and Klebsiella decreased significantly. Antibiotic treatment inhibited the gut flora of S. frugiperda , which decreased larval food intake and body weight gain, and prolonged the larval stage. CONCLUSION The composition of the gut bacterial community plays an important role in the growth, development, and feeding of S. frugiperda . The results have a certain theoretical value for the development of bio-pesticides targeting intestinal flora. © 2022 Society of Chemical Industry.
研究领域:
昆虫学
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微生物学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
Biotechnology Journal
·
2023
·
IF 4.7
Haiquan Yang,Kunjie Zhang,Wei Shen,Yuanyuan Xia,Yiting Li,Xianzhong Chen
Cembratriene-ol is a good biodegradable biopesticide ingredient with future potential applications in the field of sustainable agriculture. Cembratriene-ol is a monocyclic diterpenoid compound that is synthesized only in the trichome gland of Nicotiana plants. In this study, geranylgeranyl diphosphate synthase gene ggpps from Taxus canadensis and cbts*Δp were heterologously expressed in Saccharomyces cerevisiae W303-1A to successfully synthesize cembratriene-ol. The titer of cembratriene-ol was increased by 1.84-fold compared to the control by overexpressing the S. cerevisiae bifunctional (2E,6E)-farnesyl diphosphate synthase genes ERG20 and cbts*Δp under one promoter P GAP . The titer of cembratriene-ol in the engineered S. cerevisiae BY4741 was increased by 1.39-fold compared to the engineered S. cerevisiae W303-1A. The titer of cembratriene-ol in the engineered S. cerevisiae BY4741 was increased by 2.22-fold compared to the control by overexpressing ERG20 and cbts*Δp , respectively, using two promoters P GAP . Cembratriene-ol was found to be successfully synthesized via the integrated expression of cbts*Δp , ggpps and ERG20 on the genome of S. cerevisiae BY4741. The titer of cembratriene-ol in S. cerevisiae S25 was further increased by 1.80-fold compared to the control via dynamic control of the squalene synthase gene ERG9 . Overexpression of the genes cbts*Δp and ggpps using pY26-GPD-TEF in S. cerevisiae S25 with their integration expression increased the titer of cembratriene-ol by 26.1-fold compared to S. cerevisiae S25. The titer of cembratriene-ol was significantly enhanced by mitochondrial compartmentalized expression of cbts*Δp and ggpps , which was 76.3-fold higher than that of the initial strain constructed. It was indicated that the systematic optimization has great potential in facilitating high-level production of cembratriene-ol production in S. cerevisiae . This article is protected by copyright. All rights reserved
研究领域:
生物技术
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微生物学
·
植物科学
·
分子生物学
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代谢工程
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Jinsheng Xu,Lin Zheng,Mingtao Tan,Hongfei Wu,Shanchun Yan,Dun Jiang
Heavy metal pollution is an abiotic factor that can affect the efficiency of pest control. In this study, two microbial pesticides , Bacillus thuringiensis and Mamestra brassicae nuclear polyhedrosis virus (MbNPV), were used to treat Hyphantria cunea larvae with Cd pre-exposure, and the humoral and cellular immunity of H. cunea larvae with Cd exposure were evaluated. The results showed that Cd exposure increased the susceptibility of H. cunea larvae to microbial pesticides B. thuringiensis and MbNPV, and the lethal effect of Cd exposure and microbial pesticides on H. cunea larvae was synergistic. Cd exposure significantly decreased the expression of pathogen recognition genes (GNBP1 and GNBP3), signal transduction genes (Relish, Myd88, Tube, and Imd), and antimicrobial peptide gene (Lebocin) in the humoral immunity of H. cunea larvae compared with the untreated larvae. Parameters of cellular immunity, including the number of hemocytes , phagocytic activity , melanization activity, encapsulation activity, and the expression of three phagocytic regulatory genes (HEM1, GALE1, GALE2), were also found to decrease significantly in Cd-treated larvae. TOPSIS analysis showed that humoral immunity, cellular immunity, and total immunity levels of H. cunea larvae with Cd exposure were weaker than those in untreated larvae. Correlation analysis showed that the mortality of two microbial pesticides investigated in H. cunea larvae was negatively correlated with the humoral and cellular immunity of larvae. Taken togther, Cd exposure results in immunotoxic effects on H. cunea larvae and the use of microbial pesticides are an effective strategy for pest control in heavy metal-polluted areas.
研究领域:
昆虫学
·
环境科学
·
毒理学
·
害虫管理
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF MICROBIOLOGY
·
2022
·Vol. 204
·期 3
·1-12
·
IF 2.667
Ming Shanxiu,Chen Xujun,Zhang Na,Li Shuyi,Zhu Zhenzhou,Cheng Shuiyuan
Microorganisms can produce many antibiotics against bacteria and fungi, which have been used as a potential choice of new antibiotics. In this paper, we studied the characteristics of antibacterial substances by Bacillus cereus BC1. The results showed that the acid-precipitated substance played the main role in antibacterial activity, and further characterization indicated that the antibacterial substance might be a lipopeptide substance. Then the antibacterial spectrum suggested that the antibacterial substance had an inhibitory effect on Gram-positive bacteria and fungi, while selenium-riched antibacterial substance of Bacillus cereus BC1 could significantly enhance the inhibition. Then the morphological effects of the antibacterial substance to indicator bacteria were determined. The effects of different treatment methods on the stability of antibacterial substances were studied and the results showed that the antibacterial substance was stable to heat, ultrasonic, and ultraviolet treatment, and their antibacterial activity would not be greatly affected. However, they were sensitive to pepsin. The optimum pH range of antibacterial activity was 3–5. This study may contribute to reusing the fermentation supernatant often discarded in the previous fermentation process. At the same time, the lipopeptide antibacterial substance extracted from the fermentation broth of selenium-enriched Bacillus cereus BC1 can be used in the development of antibiotics and biopesticides, and open up a new way for the control of plant diseases.
研究领域:
微生物学
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生物技术
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药学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
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