ACS Biomaterials Science & Engineering
·
2020
·
IF 4.152
Yinan Qu,Tianyi Gu,Qiaolin Du,Changyu Shao,Jing Wang,Biao Jin,Weijing Kong,Jian Sun,Chaoqun Chen,Haihua Pan,Ruikang Tang,Xinhua Gu
Biomineralization has intrigued researchers for decades. Although mineralization of type I collagen has been universally investigated, this process remains a great challenge due to the lack of mechanistic understanding of the roles of biomolecules. In our study, dentine was successfully repaired using the biomolecule polydopamine (PDA), and the remineralized dentine exhibited mechanical properties comparable to those of natural dentine. Detailed analyses of the collagen mineralization process facilitated by PDA showed that PDA can promote intrafibrillar mineralization with a decreased heterogeneous nucleation barrier for hydroxyapatite (HAP) by reducing the interfacial energy between collagen fibrils and amorphous calcium phosphate (ACP), resulting in the conversion of an increasing amount of nanoprecursors into collagen fibrils. The present work highlights the importance of interfacial control in dentine remineralization and provides profound insight into the regulatory effect of biomolecules in collagen mineralization as well as the clinical application of dentine restoration.
研究领域:
牙科材料学
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生物医学工程
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口腔医学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wen Ma,Mingxia Liu,Mengling Tu,Gang Wang,Jun Li,Fen Liu,Jian Yang,Xianjiang Li
Five two-dimensional multi-layered MXenes namely MO 2 C, Ti 2 C, V 2 C, Nb 2 C and Ta 2 C were synthesized by a one-step exfoliation process and evaluated as laser desorption ionization mass spectrometry (LDI-MS) matrices for fast detection of small biomolecules for the first time. Among them, Nb 2 C exhibited the best performance due to its high carrier mobility, strong photo-induced charge transfer resonance and good UV absorption ability. A simple, universal and efficient LDI-MS platform was established for small biomolecule analysis based on the Nb 2 C matrix. Key factors that affect LDI-MS efficacy were investigated and the established method demonstrated ultraclean MS background, high sensitivity with detection limits low to fmol level and good reproducibility. The Nb 2 C-based LDI-MS platform was successfully applied in the rapid determination of 13 amino acids (AAs) in human serum and 3 AAs, as potential biomarkers, were found to be significantly down-regulated in major depressive disorder patients. This work proved the feasibility of Nb 2 C MXene as an LDI-MS matrix and provided a promising and universal platform for the rapid detection of small molecules in biomedical research.
研究领域:
分析化学
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生物医学
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材料科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-8
·
IF 5.479
Yang Hongmei,Su Rui,Wishnok John S.,Liu Ning,Chen Changbao,Liu Shuying,Tannenbaum Steven R.
Magnetic silica nanoparticles (MSNPs) were prepared and applied for the first time as a matrix in MALDI MS for analysis of small thermally labile biomolecules including oligosaccharides, amino acids, peptides, nucleosides, and ginsenosides. The matrix was characterized by scanning electron microscopy and UV-vis spectroscopy. It displays good performance in analyses of such biomolecules in the positive ion mode. In addition, the method generates significantly less energetic ions compared to the use of carbon nanotubes or graphene-assisted LDI MS and thus produces intact molecular ions with little or no fragmentation. In addition, the MSNPs have better surface homogeneity and better salt tolerance and cause lower noise. It is assumed that the soft ionization observed when using MSNPs as a matrix is due to the specific surface area and the homogenous surface without large clusters. The matrices were applied to the unambiguous identification and relative quantitation of the water extract of Panax ginseng roots. Any false-positive results as obtained when using graphene and carbon nanotubes as a matrix were not observed.
Schematic presentation of the application of magnetic silica nanoparticles in laser desorption ionization mass spectrometry. Their use results in little or no fragmentation during analysis of small labile biomolecules with some advantages such as better surface homogeneity, high salt tolerance, and lower noise.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2024
·
IF 10.7
Yujing Liang,Yifei Wang,Mengdi Yu,Yuqian Jiang,Jinhua Shang,Xiaoqing Liu,Fuan Wang
Biomolecules play essential roles in regulating the orderly progression of biochemical reaction networks. DNA-based biocircuits supplement an attractive and ideal approach for the visual imaging of endogenous biomolecules, yet their sensing performance is commonly encumbered by the undesired signal leakage. To solve this issue, here we proposed a glutathione (GSH)-activated DNA circuit for achieving the spatio-selective microRNA imaging through the successive response of a GSH-specific activation procedure and a non-enzymatic catalytic signal amplification procedure. In this design, by incorporating a disulfide bond into the pre-sealed nucleic acid probe, the uncontrolled circuitry leakage could be effectively ameliorated. In target cancer cells with high-abundant GSH and miR-21, endogenous GSH recognized and cleaved the pre-installed disulfide bond within DNA probes, thereby restoring the activity of circuitry components. The miR-21 then catalyzed the specific operation of circuitry for generating an amplified readout signal. We demonstrate that this system not only enables the effective discriminations of various cell types, but also contributes to the exploration of the correlationship between GSH and miR-21. This on-site activated DNA circuit can be extended to the robust analysis and exploration of different biomolecular interactions, offering a reliable reference for the in-depth understanding of biochemical interaction networks.
研究领域:
生物化学
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分子生物学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Zhi-Gang Wang,Yusi Hu,Hao-Yang Liu,Hai-Yan Wen,Bao-Ping Qi,Shu-Lin Liu
Electrochemiluminescence (ECL) imaging, a rapidly evolving technology, has attracted significant attention in the field of cellular imaging. However, its primary limitation lies in its inability to analyze the motion behaviors of individual particles in live cellular environments. In this study, we leveraged the exceptional ECL properties of quantum dots (QDs) and the excellent electrochemical properties of carbon dots (CDs) to develop a high-brightness ECL nanoprobe (CDs-QDs) for real-time ECL imaging between living cells. This nanoprobe has excellent signal-to-noise ratio imaging capabilities for the single-particle tracking (SPT) of biomolecules. Our finding elucidated the enhanced ECL mechanism of CDs-QDs in the presence of reactive oxygen species through photoluminescence, electrochemistry, and ECL techniques. We further tracked the movement of single particles on membrane nanotubes between live cells and confirmed that the ECL-based SPT technique using CD-QD nanoparticles is an effective approach for monitoring the transport behaviors of biomolecules on membrane nanotubes between live cells. This opens a promising avenue for the advancement of ECL-based single-particle detection and the dynamic quantitative imaging of biomolecules.
研究领域:
细胞生物学
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分析化学
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DOI
PubMed
高影响因子
Scientific Reports
·
2015
·Vol. 5
·期 1
·1-4
·
IF 5.578
Yin Yongqi,Sun Ye,Yu Miao,Liu Xiao,Jiang Tingting,Yang Bin,Liu Danqing,Liu Shaoqin,Cao Wenwu
ZnO nanorods (NRs) are known for ultra-sensitive biomolecule detection through fluorescence enhancement. In this work, we demonstrate that ZnO NR arrays grown on Ag layers can significantly improve the enhancement up to 86 times compared to that grown on bare Si and the enhancement can be modified in a controlled manner by varying Ag thickness. The much improved waveguide properties are attributed to the high reflectance of the Ag layers and their tuning effect on the diameters of ZnO NRs. Our results provide a deep insight into the mechanism of NRs-based fluorescence enhancement platform.
研究领域:
材料科学
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纳米技术
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光学
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生物传感
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Wenjing Zhang,Yue Han,Shuxin Li,Shengyu Wu,Juanfen Chai,Jincai Ma,Dayi Zhang,Yuesuo Yang
The diversity of colloidal types and the differences in the composite ratios in porous media are important factors governing the migration and biological risk of pathogenic microorganisms in the subsurface environment. In this study, E. coli O157:H7 was subjected to co-migration experiments with different compositions of the composite colloid montmorillonite (MMT)-Fe 2 O 3 , and the biomolecular response of E. coli under the action of colloids was analyzed by Raman spectroscopy to quantify the risk of E. coli under the action of composite colloids based on both. The results showed that Fe 2 O 3 colloids inhibited E. coli migration mainly by electrostatic adsorption and reduced E. coli metabolism. MMT colloid inhibited E. coli migration mainly by blockage, and E. coli metabolism increased, and surface macromolecules decreased to reduce E. coli adhesion. MMT-Fe 2 O 3 complex colloids inhibited migration through electrostatic attraction between the two and formation of cohesive colloids, with reduced E. coli metabolism and insignificant biomolecular response. It was briefly assessed that the composite colloids reduced E. coli risk less strongly than single colloids, stemming from the difference in the mechanism of influence and the actual need to consider colloid interactions. This conclusion can inform the management and control of pathogen risk in porous media environments.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yue Wang,Yingjie Zhao,Yingfei Ma,Dengchao Wang
Carbon nanopipettes (CNPs) have attracted much attention in nanoscale electrochemical applications recently, while the carbon structure and surface oxygen-containing groups limit its applications. Herein, we grow the carbon nanotubes (CNTs) inside the quartz nanopipet via the chemical vapor deposition method, and the fabricated carbon nanotube nanopipettes (CNT-NPs) exhibit better electrochemical responses toward biomolecules such as glutathione and ascorbic acid, compared to the conventional CNPs. In addition, the carbon nanopipette can also be easily doped by a chemical reaction with urea, to display positive surface charges and high electrochemical activity for H2O2 oxidation/reduction. This work provides an easy way to tailor the surface structure and charges of the deposited carbon inside the pipettes and thus would further promote its broader usage in electrochemical sensing applications in biological fields.
研究领域:
纳米技术
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生物医学工程
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电化学传感
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2020
·
IF 6.551
Zhicheng Wu,Xiaobao Su,Zhang Lin,Nasreen Islam Khan,Gary Owens,Zuliang Chen
While iron-based nanoparticles ( n Fe) prepared using green tea extracts have been successfully used to degrade many organic contaminants , their application to remove As(V) remains limited. Thus, in this work, n Fe (GT-1) prepared using a green tea extract was used to removal As(V). The maximum adsorption capacity of GT-1 for As(V) was 19.9 mg g −1 at 298 K. The formation of GT-1 and the removal mechanism of As(V) by GT-1, was examined using XRD, TEM and SEM, which showed that GT-1 was composed of amorphous particulates sized between 50 and 100 nm. GC–MS and LC-MS analysis also showed that biomolecules presented in the green tea extract, including polyphenols and L-theanine, participated in the formation of GT-1. Mössbauer spectral analysis confirmed that an organo-Fe(III) complex was formed due to the reaction between biomolecules and Fe(III). FTIR and XPS showed that the adsorption of As(V) by GT-1 occurred both via complexation with Fe(III) in GT-1 and via coordination of As(V) with free hydroxyl groups on the surface of GT-1. Batch experiments showed that adsorption was spontaneous and conformed to the pseudo-second order kinetic model. Finally, mechanisms for the formation of GT-1 and the removal of As (V) by GT-1 were proposed.
研究领域:
环境科学
·
材料科学
·
化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Lu Chen,Liping Song,Yichi Zhang,Ping Wang,Zhidong Xiao,Yuguo Guo,Feifei Cao
Nitrogen (N) and sulfur (S) codoped reduced graphene oxide (N,S-rGO) was synthesized through a facile solvothermal process. The introduction of N and S heteroatoms into GO effectively activated the sp2-hybridized carbon lattice and made the material an ideal electron/energy acceptor. Such unique properties enable this material to perform as a general platform for rapid and sensitive detection of various biological species through simple fluorescence quenching and recovering. When quantum dot (QD)-labeled HBV (human being disease-related gene hepatitis B virus DNA) and HIV (human being disease-related gene human immunodeficiency virus DNA) molecular beacon probes were mixed with N,S-rGO, QD fluorescence was quenched; when target HBV and HIV DNA were added, QD fluorescence was recovered. By the recovered fluorescence intensity, the target virus DNA detection limits were reduced to 2.4 nM for HBV and 3.0 nM for HIV with detection time of less than 5 min. It must be stressed out that different viruses in the same homogeneous aqueous media could be discriminated and quantified simultaneously through choosing diverse QD probes with different colors. Moreover, even one mismatched target DNA could be distinguished using this method. When altering the molecular beacon loop domain to protein aptamers, this sensing strategy was also able to detect thrombin and IgE in 5 min with detection limits of 0.17 ng mL–1 and 0.19 ng mL–1, respectively, which was far more rapid and sensitive than bare GO-based fluorescence detection strategy.
研究领域:
材料科学
·
生物化学
·
医学检测
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-9
·
IF 4.259
Hu Yalei,Geng Xin,Zhang Lin,Huang Zhongming,Ge Jia,Li Zhaohui
In this report, a novel fluorescent sensing platform using nitrogen-doped carbon dots (N-CDs) as probes for fluorescence signal transmission has been designed for the detection of significant biomolecules pyrophosphate (PPi) and alkaline phosphatase (ALP). The high fluorescent N-CDs could be selectively quenched by Cu 2+ , and recovered by the addition of PPi because PPi preferentially binds to Cu 2+ . Once ALP was introduced into the system, ALP can specifically hydrolyze PPi into Pi, the intense fluorescence of N-CDs could be quenched again due to the recombination of the as-released Cu 2+ with N-CDs. So, fluorescence of N-CDs is regulated by an ALP-triggered reaction. Based on this strategy, we demonstrated that N-CDs could serve as a very effective fluorescent sensing platform for label-free, sensitive and selective detection of PPi and ALP with low detection limit of 0.16 μM and 0.4 U/L for PPi and ALP, respectively. Moreover, the assay time is just around 0.5 min for PPi and 30 min for ALP. This developed strategy shows remarkable advantages including sensitive, rapid, simple, convenient, and low-cost and so forth. Furthermore, this method was also successfully applied to monitor ALP in human serum, which indicates its great potential for practical applications in biological and clinical diagnosis.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2013
·Vol. 15
·期 6
·1-11
·
IF 2.175
Jiang Xinde,Sun Daohua,Zhang Genlei,He Ning,Liu Hongyu,Huang Jiale,Odoom-Wubah Tareque,Li Qingbiao
The effects of different biomolecules in Artocarpus heterophyllus Lam leaf extract on the morphology of obtained gold nanoparticles were investigated in this study. The results indicated that reducing sugars, flavones, and polyphenols consisting of about 79.8 % dry weight of the leaf extract were mainly involved in providing the dual function of reduction and the size/shape control during the biosynthesis. The gold nanoparticles present included 64 ± 10 nm nanospheres, 131 ± 18 nm nanoflowers, and 347 ± 136 nm (edge length) nanoplates and they were synthesized using the main content of reducing sugars, flavones, and polyphenols, respectively, after they were desorbed by the AB-8 macroporous adsorption resin column. Particularly, flower-like and triangular/hexagonal gold nanoparticles with a yield more than 80 % were obtained. Possible shape-directed agents for the nucleation and growth were characterized by FTIR, it can be seen that ketones were bound on the surface of the spherical and flower-like GNPs, while both the ketones and carbonyls bound on the Au {111} plane this may have favored the formation of the twin defects, which are very essential for nanoplates’ formation.
研究领域:
植物学
·
纳米生物学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）