Current Pharmaceutical Analysis
·
2018
·Vol. 14
·期 1
·41-52
·
IF 0.859
Sun Congyong,Yuan Yangyang,Omari-Siaw Emmanuel,Tong Shanshan,Zhu Yuan,Wang Qilong,Wang Yuanwen,Wei Qiuyu,Yu Jiangnan,Xu Ximing
Objective: This study aims to establish an efficient, sensitive and specific analytical method (in vitro and in vivo) for syringic acid. Method: Syringic acid loaded liposome was prepared and characterized. In vitro and in vivo chromatographic conditions for the determination of
syringic acid were investigated, with the application verified by pharmacokinetics and biodistribution studies. Results: The developed liposomal formulation produced homogeneous regular, spherical and multilamellar- shaped liposome. Efficient HPLC method for the analysis of syringic acid
was established and validated. The chromatographic separation was performed on a Waters symmetry C18 column (4.6 150 mm, 5 μm) at 25 °C by using mobile phases of 30 % methanol and 70 % acetic acid solution (0.1 %) (in vitro) and 28 % methanol and 72 % acetic acid solution (0.1 %) (in
vivo) at a flow rate of 0.8 mL/min. The wavelength was set at 272 nm. Vanillin was used as internal standard for the in vivo analysis. The established linearity ranges of SA were 1 - 100 μg/mL (R2 = 0.9997) in vitro and 0.125 – 64 μg/mL (R2 = 0.9991) in vivo. In vitro accuracy
of the method was > 99%, and in vivo accuracies were between 91.23 % - 109.54 %, while the precisions met the acceptance criterion. In vivo HPLC method was also confirmed by pharmacokinetics and biodistribution studies. Conclusion: Established HPLC method exhibited high linearity,
precision, accuracy and specificity, could be used for quantitative determination of SA in vitro and in vivo.
研究领域:
药物分析
·
药理学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2014
·Vol. 302
·期 1
·123-130
·
IF 1.415
Sun Zhi-Zhong,Wei Hong-Yuan,Wang Guan-Quan,Zhou Zhi-Jun,He Jia-Heng,Wang Jing,Luo Shun-Zhong
[ 131 I]-5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin and [ 131 I]-5-(4-aminophenyl)-10,15,20-triphenylporphyrin ( 131 I-TPPOH and 131 I-TPPNH 2 ) were prepared and their biodistribution properties were evaluated in normal Kunming (KM) mice and SMMC-7721 tumor-bearing BALB/c mice. The optimized labeling yields were 98 and 96 % for 131 I-TPPOH and 131 I-TPPNH 2 , respectively. They were stable in vitro saline and in vivo. Both compounds had a specific affinity to liver and lung, and mainly metabolized through liver. They showed a time-dependent accumulation and retainable characteristics in SMMC-7721 tumor. These results supported their potential value for targeted tumor therapy agents.
研究领域:
放射医学
·
肿瘤学
·
药理学
✓ 已验证
DOI
高影响因子
Food & Function
·
2017
·Vol. 8
·期 7
·2558-2565
·
IF 3.247
Yao Li,Jingran Bi,Shan Liu,Haitao Wang,Chenxu Yu,Dongmei Li,Bei-Wei Zhu,Mingqian Tan
The presence of nanomaterials during food processing has attracted significant concern due to the physicochemical properties of nanomaterials. In this study, the presence and formation of nitrogen-containing fluorescence carbon dots (C-dots) in a grilled hamburger at different temperatures (220, 260, and 300 °C) were investigated during the pyrolysis process. The size and morphology of the C-dots were found to be highly dependent on the heating temperatures, which again affected the functional groups on their surface. The C-dots are strongly fluorescent with multicolor emission accompanied by a gradual decrease in fluorescence intensity. The fluorescence quantum yield of the C-dots produced at 260 °C was measured to be 23.25%. The potential cytotoxicity and biodistribution of the C-dots within live organisms were examined with the mouse osteoblasts cell line and mung bean sprout, respectively. The cell viability after 24 h incubation remained 79% for the C-dots obtained at 300 °C at a concentration of 3.2 mg mL−1, and no obvious phytotoxicity in the growing mung bean sprout was observed. The results showed an increased cytotoxicity of the C-dots formed at higher temperatures.
研究领域:
食品科学
·
毒理学
·
纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2018
·
IF 6.383
Doudou Hu,Ting Li,Zongpu Xu,Di Liu,Mingying Yang,Liangjun Zhu
A variety of colloid stabilizers and cryoprotectants confer improved nanoparticle (NP) colloidal stability and redisperability. However, discounted tumor targetability, delivery efficacy and possible side effects limit the application in vascular delivery of NPs. Here we present water-soluble silk sericin (SS) not only as a material for the preparation of NPs, but also both a dispersion stabilizer and a cryoprotectant. In the absence of any stabilizers, SS-based NPs (SSC@NPs) can resist the adsorption of serum proteins , preventing the formation of particle agglomerates. Following freeze-drying without addition of cryoprotectants, SSC@NPs powder can be easily resuspended into NP dispersion with a nearly monodispersed distribution. Additionally, SSC@NPs do not result in acute toxicity in mice at a dose of 400 mg/kg with a slow injection. Moreover, doxorubicin (DOX)-loaded SSC@NPs (DOX-SSC@NPs) diminish the biodistribution of DOX in the heart, mitigating DOX-induced cardiotoxicity of mice without compromising therapeutic efficacy. Our results suggest that the self-stabilized SSC@NPs could be a secure and effective drug carrier for intravenous administration when deprived of protective agents. Statement of Significance During manufacturing process such as freeze-drying, or interaction with complex fluids like blood, NPs for systemic drug delivery need to be highly dispersible and structurally intact. In this work, we have demonstrated the self-stability of SSC@NPs subjected to biological media and freeze-drying. This study represents the first work showing water-soluble SS could both act as a dispersion stabilizer and a cryoprotectant due to its hydrophilicity . Plus, good in vivo biocompatibility of SSC@NPs has been confirmed. Therefore, it may be promising that water-soluble SS can be generally used as a safe biomaterial against serum adsorption.
研究领域:
药剂学
·
生物医学工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Lixin Ding,Qian Guo,Ya’nan Ren,Pei Wang,Yongxiang Pan,Jin Ding,Feng Wang,Zhi Yang,Hua Zhu
Extracellular vesicles (EVs) act as carriers that transfer molecules between donor and recipient cells, thereby altering the phenotype and function of the latter. EVs derived from mesenchymal stem cells (MSCs) inherit the homing ability of their parent cells to tumor sites. We speculate that MSC-derived EVs labeled with the isotope zirconium-89 ([89Zr]Zr) will accumulate within tumors and have the potential for tumor location via positron emission tomography (PET) imaging. Here, as a proof of concept, we used [89Zr]Zr-labeled human umbilical cord MSC (hucMSC)-derived EVs to characterize the homing of EVs to tumor regions via PET imaging. The uptake of [89Zr]Zr-DFO-hucMSC-EVs by 4T1, H292, and FaDu cells increased in a time-dependent manner. [89Zr]Zr-DFO-hucMSC-EVs were rapidly cleared from the blood circulation, showing a 2-phase exponential decay with a biodistribution half-life of 0.46 h and an elimination-phase half-life of 11.87 h. At 24 h postinjection, [89Zr]Zr-DFO-hucMSC-EVs were mainly distributed in the liver (10.39 ± 0.52%ID/g) and, to a lesser extent, in the spleen (9.87 ± 0.87%ID/g). PET imaging in tumor-bearing mouse models revealed persistently high tumor accumulation. The projected effective dose for an adult human female was low and was 0.084 mSv/MBq. There was no obvious normal tissue toxicity following the administration of excessive radioactivity. In summary, these studies demonstrate the potential feasibility of using the [89Zr]Zr-DFO-hucMSC-EV tracer for noninvasive visualization of tumor lesions.
研究领域:
医学影像学
·
细胞生物学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Huaqiao Tang,Min Xu,XueRong Zhou,Yuanli Zhang,Ling Zhao,Gang Ye,Fei Shi,Cheng Lv,Yinglun Li
In order to compare the detailed toxicity of nano‑copper and CuCl 2 ·2H 2 O (Cu ions) in vivo , the oral toxicity of four differently sized Cu particles (30 nm, 50 nm, 80 nm and 1 μm) on rats was investigated compared with CuCl 2 ·2H 2 O in acute exposure scenarios. We compared the acute LD 50 values and evaluated the kinetics of Cu following a single equivalent dose (200 mg/kg) of five Cu materials. Continuous gavage of nano‑copper for 7 days, the mortality rates, relative organ weights, and hematological, biochemical, and histopathologic characteristics of rats were examined. The results showed that the LD 50 values of Cu ions, 30 nm, 50 nm, 80 nm, and 1 μm copper particles were 359.6, 1022, 1750, 2075, and >5000 mg/kg, respectively. Physiological and biochemical indexes indicated that 80 nm nano‑copper (Cu NPs) produced the highest degrees of toxicity in short term. The liver and kidneys were the major organs most affected by Cu NPs, and also the target organs for Cu accumulation. The toxic effects of Cu ions are similar to those of nano‑copper, but they were not the same. Therefore, the toxic effect of nano‑copper is likely to be the result of the dual action of nano‑copper particles and copper ions. Collectively, the acute toxic effects produced by Cu NPs were highly correlated with particle size. Moreover, the toxic effects produced by repeated dosing differed from those produced by a single dose, and this may be due to organ targeting effects that are dependent on the size of the nano-particles.
研究领域:
毒理学
·
纳米生物学
·
环境科学
✓ 已验证
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2018
·
IF 4.432
Ying Duan,Ruiping Duan,Rui Liu,Man Guan,Wenjuan Chen,Jingjing Ma,Mingmao Chen,Bo Du,Qiqing Zhang
Biocompatible, near-infrared luminescent gold nanoclusters were synthesized in situ using as-prepared chitosan grafted with N-acetyl-l-cysteine (NAC-CS). The fluorescent gold nanoclusters coated with chitosan-N-acetyl-l-cysteine (AuNCs@NAC-CS) were aggregated by multiple ultrasmall gold nanoclusters closing with each other, with strong fluorescence emission at 680 nm upon excitation at 360 nm. AuNCs@NAC-CS did not display any appreciable cytotoxicity on cells even at a concentration of 1.0 mg mL–1. AuNCs@NAC-CS were more insensitive to H2O2 and trypsin compared with fluorescent gold nanoclusters coated with Albumin Bovine V (AuNCs@BSA), which make them have long time imaging in HeLa cells. Furthermore, the obvious fluorescence signal of AuNCs@NAC-CS appeared in the liver and kidney of the normal mice after 6 h injection. And the fluorescence intensity decreased after that because of the highly efficient clearance characteristics of ultrasmall nanoparticles. These findings demonstrated that AuNCs@NAC-CS possessed good fluorescence, low cytotoxicity, and low sensitivity to some content of cells, allowing imaging of the living cells.
研究领域:
生物医学
·
纳米技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 22
·12260-12268
·
IF 2.936
Meiyu Wu,Liang Chen,Ruiru Li,Mo Dan,Haining Liu,Xinsheng Wang,Xiaochun Wu,Ying Liu,Liming Xu,Liming Xie
Along with the increasing applications of nanomaterials in medical fields, to know the systemic distribution of nanomaterials in the body through a precise method is required for the biosafety assessment of nanomaterials. In this study, we firstly have established a reliable inductively coupled plasma mass spectrometry (ICP-MS) method for concentration measurement of silver (Ag) and gold (Au) in biological tissues. Then, based on this method, the Ag or Au distribution in rat blood and almost all of the organs were analyzed after an i.v. or s.c. administration of Au@Ag NRs. Both the time-dependent contents of Ag and Au in blood and two pharmacokinetic models confirmed the rapid clearance of Ag from blood. At 24 h after i.v. injection, there was the highest level of Ag in liver, followed by portal nodes, spleen, lung, bone marrow and pancreas. In addition, we also found there were gender-related distributions of Ag and Au in some organs, especially after s.c. injection. Therefore, these more comprehensive and important results would give fundamental information for the biological risk assessment of nanomaterials.
研究领域:
医学
·
毒理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Guang Ma,Xiaolin Xu,Ming Qi,Yingjian Zhang,Xiaoping Xu
Interventional therapy is very important in current clinical applications. However, the lack of quantifiable embolic agents to affect the therapeutic effects negatively. In the present study, we sought to prepare a novel embolic agent, 99m Tc-Konjac glucoman/Poly(lactide- co -glycolide)@Polyethyleneimine@Diethylenetriaminepentaacetic acid(KGM/PLGA@PEI@DTPA)microspheres, which can be located and quantified noninvasively to further evaluate its biological effects in animal models. The KGM/PLGA microspheres were prepared with water/oil/water double emulsion-solvent evaporation. Subsequently, the surface modification was performed with PEI and DTPA as a bifunctional chelator to label the microspheres with 99m Tc. The in vitro stability of 99m Tc-KGM/PLGA@PEI@DTPA was determined through instant thin layer chromatography, and the in vitro cytotoxicity of KGM/PLGA@PEI@DTPA was evaluated with CCK-8 assays. The location and quantitative calculation of 99m Tc-KGM/PLGA@PEI@DTPA in vivo were evaluated in rabbits through SPECT/CT, and the in vivo toxicity was assayed in rabbits.The microspheres showed no toxicity in vitro and in vivo , andthe diameter of microsphere was about 82.5 ± 14.5 μm(range 60–120 μm). The 99m Tc-KGM/PLGA@PEI@DTPA was stable in phosphate-buffered saline and 50% fetal bovine serum within 6 h. The Single-photon emission computed tomography/computed tomography(SPECT/CT) imaging and biodistribution results showed that microspheres were mainly distributed in the liver, kidney, and bladder, and excreted through the urinary system. And the dose of microspheres embolizing in the liver was approximately 2.54 mg, 50.79% of the total injection, an estimate calculated according to the gamma ray emission from 99m Tc in SPECT/CT.Hence, the 99m Tc-KGM/PLGA@PEI@DTPA may be as a quantitative embolic agent and provide potential clinical value for interventional therapy.
研究领域:
医学影像学
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yan Gao,Quanzhi Xiao,Jie Zhang,Kena Zhang,Liping Fang,Xiao-Xia Zhou,Bing Yan
Nanoplastics (NPs) are prevalent in the environment, posing risks to ecosystems and human health. While research into their effects on bacterial activity has increased, the mechanisms underlying NP-bacteria interactions─specifically whether NPs penetrate cells or adhere to the cell surface─remain poorly understood. This knowledge gap largely stems from the absence of quantitative analytical methods. Herein, we developed a novel approach combining lysozyme treatment with pyrolysis gas chromatography–mass spectrometry (Py-GC/MS) to differentiate between intracellular and cell wall-bound NPs in Escherichia coli (E. coli) quantitatively. The method involves selective removal of the bacterial cell wall using lysozyme, protein corona-induced extraction to enrich cell wall-bound NPs, and hydrogen peroxide digestion to eliminate protoplast interference before Py-GC/MS analysis. Validation with europium (Eu)-labeled NPs, quantified by inductively coupled plasma mass spectrometry (ICP-MS), confirmed the method’s accuracy and reliability. Using this approach, we found that after NP exposure, only a small fraction (9.6–10.5%) of NPs penetrated E. coli cells, while the majority (36.9–63.8%) adhered to the cell surface. Transmission electron microscopy further corroborated these findings. Consequently, this work provides a robust tool for the quantification of NP uptake and biodistribution in bacterial systems, advancing our understanding of NP–microorganism interactions and their environmental implications.
研究领域:
环境科学
·
微生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Talanta Open
·
2025
·
IF 3.7
Amna Naz,Sadia Haider,Mavra Rana,Neelum Malik,Attia Afzal,Muhammad Farooq,Muhammad Sarfraz
Oleanolic acid (OA) and apigenin (APG) demonstrate a wide range of pharmacological effects, including antioxidant, anti-inflammatory, antiviral, antidiabetic, hepatoprotective, and cardioprotective activities. Both compounds show anticancer potential and have been used individually to treat various cancer types, creating a rationale for their combined use. However, quantifying both drugs in quality and plasma samples presents challenges due to differences in their physicochemical properties and absorption wavelengths (OA: 210-215 nm, APG: 212, 269, and 337 nm). We developed and validated a novel High-Performance Liquid Chromatography UV detection (HPLC-UV) method to address this problem. Using a C18 column (4.6 mm x 250 mm, packing L1, 5 µm particle size) and a mobile phase of acetonitrile: methanol: tetrahydrofuran: water (60:20:8:12, v/v) with a detection wavelength of 215 nm. The method achieved retention times of 4.7 and 10 minutes for OA and APG in quality samples, and 4.9 and 9.8 minutes in plasma samples, respectively. The plasma sample was prepared by simple protein precipitation step. All validation parameters, including sensitivity, selectivity, precision, accuracy, robustness, and stability, met the required criteria. The validated HPLC-UV bioanalytical method was effectively applied in quantification, stability, and pharmacokinetic studies of co-loaded nanoformulations of OA and APG with particle size 163 nm and entrapment efficiency up to 93 %, establishing it as a promising analytical technique for detecting these anticancer agents in complex matrices such as blood, plasma, and polymeric systems.
研究领域:
药理学
·
分析化学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Zhengjie Meng,Hao Wang,Xue Fang,Zhangya Liu,Zheng Yang,Jiahui Yong,Qingqing Yang,Yunhao Bai,Hao Ren,Hao Xu,Xueming Li
The vascular wall is the first physiologic barrier that circulating nanoparticles (NPs) encounter, which also is a key biological barrier to cancer drug delivery. NPs can continually scavenge the endothelium for biomarkers of cancer, and the chance of NPs’ extravasation into the tumors can be enhanced. Here, we envision P-selectin as a target for specific delivery of drug nanocrystals to tumors. The cupric diethyldithiocarbamate nanocrystals (CuET NCs) were first prepared by an antisolvent method, and then nanocrystals were coated with fucoidan via physical interaction. The fucoidan-coated CuET nanocrystals (CuET@Fuc) possess high drug loading and have the ability to interact with human umbilical vein endothelial cells expressing P-selectin, which transiently enhances the endothelial permeability and facilitates CuET@Fuc extravasation from the peritumoral vascular to achieve higher tumor accumulation of drugs than bare CuET NCs. The CuET NC shows poorer anticancer efficacy than CuET@Fuc at the same dose of CuET. Upon repeated dosing of CuET@Fuc for 2 weeks, no mortality was observed in treated melanoma-bearing mice, while the mortality in the control group and excipient-treated groups reached 23%. The growth rate of melanoma in the CuET@Fuc-treated group was significantly lower than those in other groups. Furthermore, an acute toxicity study revealed that CuET@Fuc is a safe formulation for cancer treatment.
研究领域:
药剂学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）