Tingting Yu,Lifen Liu,Qiao Yang,Jing Song,Fenglin Yang
Using the flat module membrane electrodes based on polyester filter modified with polyaniline (PANI)–phytic acid (PA), a new type of membrane bio-reactor (MBR) system is developed for both bio-electrochemical electricity generation and effluent filtration. A specifically prepared carbon foam–Fe–Co catalyst was coated on the conductive, filterable cathode membrane which makes it catalytic for enhancing cathodic oxygen reduction reaction and electricity generation. The cell voltage (with carbon foam–Fe–Co, 0.5–0.4 V) is superior to control test 1 (without any catalyst, 0.2 V), moreover, it is even better than control test 2 (with Pt–C catalyst, 0.5–0.3 V). The corresponding maximum power density of carbon foam–Fe–Co is 38.5 and 2.4 times higher than control test 1 and 2, respectively. More importantly, the base electrode materials and catalyst were both low-cost. The overall removal efficiency of COD and NH4+–N are satisfactory, at 95% ± 2.5% and 85% ± 2.5%, respectively. This integrated system is easy to scale up for practical application in waste-water treatment and offers a better option in operating and coupling MBR with bio-electricity generation.
Tirapazamine (TPZ), an antitumor prodrug, can be activated in hypoxic environment. It specifically targets the hypoxic microenvironment of tumors and produces toxic free radicals. However, due to the tumor is not completely hypoxic, TPZ often fails to effectively treat the entire tumor tissue, resulting in suboptimal therapeutic outcomes. Herein, a low pathogenic Escherichia coli TOP10 is utilized to selectively colonize tumor tissues, disrupt blood vessels, and induce thrombus formation, leading to the expansion of hypoxic region and improving the therapeutic effect of TPZ. Additionally, a thermosensitive hydrogel is constructed by Pluronic F-127 (F127), which undergoes gelation in situ at the tumor site, resulting in sustained release of TPZ. To monitor the therapeutic process, it is genetically modified TOP10 by integrating the bioluminescent system luxCDABE (TOP10-Lux). The bioluminescent signal is associated with tumor hypoxia enhancement and thrombus formation, which is beneficial for therapeutic monitoring with bioluminescence imaging. In the murine colon cancer model, the TOP10-Lux combined with TPZ-loaded F127 hydrogel effectively suppressed tumor growth, and the treatment process is efficiently monitored. Together, this work employs genetically modified TOP10-Lux to enhance the therapeutic efficacy of TPZ and monitor the treatment process, providing an effective strategy for bacteria-based tumor-targeted chemotherapy and treatment monitoring.
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