Nanomedicine
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2014
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IF 5.824
Bates Ferdia
Nanomedicine is one of the most dynamic, lucrative and productive contemporary research streams; it is playing an intimate role in the progression of modern medicine through the development of new and highly innovative treatments as well as improving and enhancing established techniques. But what is nanomedicine and why the pomp and ceremony? This chapter aims to dissect nanomedicine with the intention of both defining it as a field and demonstrating its worthiness to receive the title of ‘revolutionary interdiscipline’. With this in mind, the field is introduced as combination of three distinct subsections: nanobiotechnology, nanotechnology and nanobiomimetics, and also through the two main synthetic approaches, top-down and bottom-up, both of which are pivotal in separating the enhancement of existing technology from the development of revolutionary new technology.
研究领域:
纳米医学
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生物医学工程
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分子医学
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转化医学
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药剂学
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生物材料
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纳米生物技术
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再生医学
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2024
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IF 3.4
Caixia Pei,Xinlv Peng,Yiran Wu,Runmiao Jiao,Tiehai Li,Siming Jiao,Lei Zhou,Jianjun Li,Yuguang Du,Eika W. Qian
Eukaryotic sialyltransferases play key roles in many physiological and pathological events. The expression of active human recombinant sialyltransferases in bacteria is still challenging. In the current study, the genes encoding human N -acetylgalactosaminide α2,6-sialyltransferase V (hST6GalNAc V) and N -acetylgalactosaminide α2,6-sialyltransferase VI (hST6GalNAc VI) lacking the N -terminal transmembrane domains were cloned into the expression vectors, pET-32a and pET-22b, respectively. Soluble and active forms of recombinant hST6GalNAc V and hST6GalNAc VI when coexpressed with the chaperone plasmid pGro7 were successfully achieved in Escherichia coli . Further, lactose (Lac), Lacto- N -triose II (LNT II), lacto- N -tetraose (LNT), and sialyllacto- N -tetraose a (LSTa) were used as acceptor substrates to investigate their activities and substrate specificities. Unexpectedly, both can transfer sialic acid onto all those substrates. Compared with hST6GalNAc V expressed in the mammalian cells, the recombinant two α2,6-sialyltransferases in bacteria displayed flexible substrate specificities and lower enzymatic efficiency. In addition, an important human milk oligosaccharide disialyllacto- N -tetraose (DSLNT) can be synthesized by both human α2,6-sialyltransferases expressed in E. coli using LSTa as an acceptor substrate. To the best of our knowledge, these two active human α2,6-sialyltransferases enzymes were expressed in bacteria for the first time. They showed a high potential to be applied in biotechnology and investigating the molecular mechanisms of biological and pathological interactions related to sialylated glycoconjugates.
研究领域:
分子生物学
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生物化学
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生物技术
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酶学
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糖生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Yanbin Feng,Nuanqing Wang,Qianqian Cao,Shang Li,Ying Xu,Xinyu Che,Jinfeng Zhao,Chuanlun Yang,Song Xue
Trehalose 6-phosphate (Tre6P) is a critical metabolic signaling molecule in plants, orchestrating diverse biological processes, including stress resistance and photosynthetic efficiency. Recent advancements highlight its promising role in improving crop yield. However, the efficient synthesis of Tre6P in vitro remains a major challenge. Here, we identified a trehalose-6-phosphate phosphorylase WcTre6PPase from Weissella ceti, the enzyme catalyzes the reversible synthesis of Tre6P from β-glucose 1-phosphate (βGlc1P) and glucose 6-phosphate (Glc6P), with a strong catalytic bias for Tre6P synthesis. The crystal structure of WcTre6PPase resolved in complex with βGlc1P reveals critical molecular determinants for substrate recognition and catalytic efficiency. Binding studies demonstrate a higher affinity of WcTre6PPase for βGlc1P by 1.0 ± 0.2 mM compared to Glc6P by 24.3 ± 7.1 mM. Complementary molecular docking and dynamics simulations provide detailed insights into the catalytic mechanism. Subsequently, by integrating WcTre6PPase into a novel multienzyme cascade, comprising maltose phosphorylase, polyphosphate glucokinase, and AMP/ADP-polyphosphate phosphotransferase, and optimizing reaction conditions, we achieved a remarkable Tre6P yield of 90% from maltose. This study provides molecular insights into the function of WcTre6PPase and establishes a prospective platform for Tre6P production for agricultural applications.
研究领域:
结构生物学
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生物化学
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植物代谢
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酶学
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生物催化
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农业生物技术
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
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IF 15.7
Xia Xue,Dong Jiawei,Li Aijie,Wang Yanlin,Liu Yang,Zhu Yingfang,Xu Liang,Jing Zhiyang,Wang Jing,Zou Yan,Sun Shiyong,Wang Lu,Lu Yiqing,Soeriyadi Alex,Wang Xuelu,Patrick John W.,Offler Christina E.,Zheng Meng,Song Chun-Peng,Shi Bingyang
Presence of the cell wall and the lack of streamlined pathways for cellular delivery of external agents into plants is a core challenge of plant biotechnology and crop engineering development. However, both viral and bacterial transmission have their own restrictions and the few non-heavy metal nanodelivery platforms require external forces for tissue penetration. Such dependency limits any high-throughput application considering the large plant numbers to be treated in the field or even laboratory exercises. Herein, we demonstrate Aspartic acid (Asp) decorated poly(ethylene glycol)-block-poly(2-(diisopropylamino)ethyl methacrylate) (Asp-PEG-PDPA) copolymers assembled micelles (Asp/PDPA-NP), a platform that utilises amino acid transporters (AtAAP1 and AtLHT1) as receptors for clathrin-dependent endocytosis, freely translocate to release loaded cargo into various plant tissue/cell types in a species-independent manner within ≤10 minutes through simple spray or co-culture. As proof-of-concept, abscisic acid (ABA)-loaded Asp/PDPA-NP was tested for its efficacy to confer plant drought resistance. Asp/PDPA-NP@ABA reduced the effective ABA dose down to 1 nM (one million-fold) and elicited anti-drought potency in representative eudicot (soybean) and monocot (maize) crop species. Owing to its delivery efficiency, Asp/PDPA-NP holds promise as a potent carrier for diverse chemicals and biomolecules in plant systems.
研究领域:
植物学
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纳米技术
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生物技术
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分子生物学
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农学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2024
·Vol. 29
·期 17
·4201
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IF 4.2
Wenxuan Jiang,Xiangrong Deng,Lanxian Qin,Dahai Jiang,Mengqi Lu,Kai Chen,Manqi Yang,Liangliang Zhang,Jianchun Jiang,Liming Lu
This study focused on developing an effective cell wall-breaking method forPhaffia rhodozyma, followed by utilizing subcritical fluid extraction to isolate, extract, and concentrate astaxanthin from the complex fermentation products ofP. rhodozyma. A comprehensive comparison of seven distinct methods for disrupting cell walls, including dimethyl sulfoxide treatment, lactic acid treatment, sodium hydroxide treatment,β-glucanase enzymatic digestion,β-mannanase enzymatic digestion, and a combined enzymatic treatment involving bothβ-mannanase andβ-glucanase was conducted. The results identified the lactic acid method as the most effective in disrupting the cell walls ofP. rhodozyma. The software, Design Expert, was used in the process of extracting astaxanthin from cell lysates using a subcritical extraction method. Through fitting analysis and response surface optimization analysis by Design Expert, the optimal extraction conditions were determined as follows: an extraction temperature of 41 °C, extraction frequency of two times, and extraction time of 46 min. These parameters facilitated the efficient extraction, concentration, and enrichment of astaxanthin fromP. rhodozyma, resulting in an astaxanthin concentration of 540.00 mg/L. This result can establish the foundation for its high-value applications.
研究领域:
生物技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
Water
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2025
·Vol. 17
·期 2
·179
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IF 3
Wei Xu,Xiaoping Zhang,Guichang Zhang,Xin Zhang
The treatment of wastewater using microalgae is regarded as a green and potential technology. However, its engineering application has been largely hindered because of the limitation of microalgae separation and harvesting. Therefore, immobilization technology has been widely used to embed microalgae for wastewater treatment. In this paper, sodium alginate (SA) and polyvinyl alcohol (PVA) as the common immobilized carriers were used to immobilizeankistrodesmus falcatusfor simulated slaughtering wastewater (SSW) treatment. The experimental results of the mass transfer and adsorption of immobilized carriers were found to show that the mass transfer of SA-SiO2gel balls (SS-GB) was better than PVA-SA gel balls (PS-GB) and that the adsorption of PS-GB was better than SS-GB. When immobilizing microalgae with the two kinds of carriers, it was found that SA-SiO2microalgal spheres (SS-MS) were better than PVA-SA microalgal spheres (PS-MS) for the maintenance of microalgal cell activity and that PS-MS were better than SS-MS for the resistance to biodegradation. This is because the carrier of PS-MS had a thick shell and dense structure, while the carrier of SS-MS had a thin shell and loose structure. The results of SSW treatment by PS-MS and SS-MS were found to show that the total phosphorus (TP) removal rates of PS-MS and SS-MS were 90.31% and 86.60%, respectively. This indicates that the TP removal effect of PS-MS was superior to that of SS-MS. The adsorption kinetics simulation showed that the adsorption of TP onto PS-GB was controlled by chemisorption and that the adsorption of TP onto SS-GB was controlled by physical adsorption. The chemical oxygen demand (COD) and ammonium nitrogen (NH4+-N) removal of PS-MS were 9.30% and 10.70%, respectively, and the COD and NH4+-N removal of SS-MS were 54.60% and 62.08%, respectively. This indicates that the COD and NH4+-N removal effect of SS-MS were superior to PS-MS. This is the result of the combined action of the degradation by microalgal cells and adsorption by the carrier.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
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2024
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IF 7.7
Xuran Du,Litao Wang,Hong Liang,Guanyu Chen,Jing Wu,Wenjie Xia,Dawen Gao
Bentonite is a non-metallic mineral with montmorillonite as the main component. It is an environmentally friendly mineral material with large reserves, wide distribution, and low price. Bentonite can be easily modified organically using the surfactant saponin to obtain saponin-modified bentonite (Sap-BT). This study investigates the immobilization of crude enzymes obtained from Trametes versicolor by physical adsorption with Sap-BT. Thus, saponin-modified bentonite immobilized crude enzymes (CE-Sap-BT) were developed to remove benzo[a]pyrene. Immobilization improves the stability of free enzymes. CE-Sap-BT can maintain more than 80% of activity at 45 °C and after storage for 15 d. Additionally, CE-Sap-BT exhibited a high removal rate of benzo[a]pyrene in soil, with 65.69% after 7 d in highly contaminated allotment soil and 52.90% after 6 d in actual soil contaminated with a low concentration of benzo[a]pyrene at a very low laccase dosage (0.1 U/3 g soil). The high catalytic and removal performance of CE-Sap-BT in contaminated sites showed more excellent practical application value.
研究领域:
环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2024
·
IF 7.4
Changwei Niu,Bing Wang,Yueyue Wang,Zichao Wang
The investigation focused on the removal of antibiotic resistance genes from wastewater using ferrate treatment at various sulfite concentrations. As the sulfite concentration increased from 0 to 28.5 mg/L, the total DNA removal rate treated by 10 mg/L ferrate increased from 71.45% to 96.48%. The antibiotic resistance genes , save our souls genes and class I integron abundance reduction treated by ferrate ranged from 0.02 log to 1.66 log copies/ml at different sulfite concentrations, and the antibiotic resistance gene parC removal was enhanced as the sulfite addition. When the sulfite concentration reached to 28.5 mg/L, the catalase and superoxide dismutase levels were decreased by 21.57% and 10.04%, respectively, while the reactive oxygen species and lactate dehydrogenase levels were increased by 75.34% and 28.67%, respectively, compared to those in the wastewater treated by alone ferrate without sulfite. In the coupled ferrate (10 mg/L) and sulfite (28.5 mg/L) system, sulfate radicals and hydroxyl radicals were the main contributors to the DNA removals, and their contribution rates were 51.99% and 28.95%, respectively. The antibiotic resistance genes and save our souls genes hosts changes showed microbial community significantly affected the DNA removal. This study offers novel comprehensions on the antibiotic resistance genes removal from the wastewater by the combined ferrate and sulfite.
研究领域:
环境科学
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水处理
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微生物学
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生物技术
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Zhiguo Wu,Dan Liu,Ying Deng,Runyi Pang,Jinjin Wang,Tian Qin,Zongzheng Yang,Rongliang Qiu
In this research, a new material, chitosan/polypyrrole (CS/PPy), was synthesized and linked with the Cr(VI)-reducing bacterial strain YL3 to treat Cr(VI)-polluted soil. The findings demonstrated that the synergistic application of strain YL3 and CS/PPy achieved the greatest reduction (99.6 %). During the remediation process, CS/PPy served as a mass-storage and sustained release agent in the soil, which initially decreased the toxic effects of high concentrations of Cr(VI) on strain YL3, thereby enhancing the Cr(VI) reduction efficiency of strain YL3. These combined effects significantly mitigated Cr(VI) stress in the soil and restored enzyme activities . Furthermore, wheat growth in the treated soil also significantly improved. High-throughput sequencing of the microorganisms in the treated soil revealed that CS/PPy was not only effective at removing Cr(VI) but also at preserving the original microbial diversity of the soil. This suggests that the combined treatment using strain YL3 and CS/PPy could rehabilitate Cr(VI)-contaminated soil, positioning CS/PPy as a promising composite material for future bioremediation efforts in Cr(VI)-contaminated soils.
研究领域:
环境科学
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生物技术
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土壤学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2024
·
IF 7.4
Zeqi Zheng,Weitao Liu,Jiantao Li,Miao Yu,Aurang Zeb,Qixing Zhou,Maohong Lin
Trichloromethane (TCM) in soil causes adverse effects on the soil ecosystem and human health. Therefore, it is urgent to develop an efficient and low-cost soil TCM removal technology. In this study, we prepared sodium alginate-biochar-laccase immobilized pellets (SA-BC-LC) using composite immobilization technology, exploring the fitting impact of artificial neural network model (ANN) and general linear model (GLM) models on laccase loading and the correlation properties of SA-BC-LC. The SA-BC-LC exhibited a porous multi-layer network structure internally. Compared to free laccase, the relative activity of SA-BC-LC remained above 50 % after 5 reuses and still maintained at 48 % after 50 days of storage. Moreover, SA-BC-LC demonstrated significant capacity for adsorption and degradation of TCM in soil with ratios approximately at 31.3 % and 68.7 %, respectively. During the remediation process of TCM-contaminated soil from Taizhou Chemical plant, there was a rapid decrease in TCM concentration within the first hour. Under IM (Immobilization) treatment (added with 5 % SA-BC-LC), the removal rate of TCM reached up to 88.9 % in the first hour. The adsorption of TCM by SA-BC-LC was mainly chemical adsorption, which induced the improvement of the relative abundance of the co-metabolic dechlorinated microbial Pseudomonas . Our research presents a promising material for effectively remediating soils contaminated with chlorinated hydrocarbons (CHs), with excellent removal efficiency.
研究领域:
环境科学
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生物技术
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土壤修复
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yumin Wang,Songbai Liu
Conventional radical grafting of proteins with catechins consumed the most antioxidant-active hydroxyls during grafting, thus failing to effectively retain antioxidant activity in conjugates. In this study, a novel strategy of selective protection of the most reactive hydroxyls before grafting was developed to preserve the most reactive hydroxyls and effectively retain antioxidant activity in conjugates. Selective protection of the most reactive hydroxyls of (−)-epigallocatechin-3-gallate (EGCG) was successfully realized in a yield of 87% applying trimethyl orthopropionate and catalytic calcium triflate at 40 °C. The novel ovalbumin (OVA)–EGCG conjugate with 93% grafting ratio was prepared by radical grafting with the selectively protected EGCG and subsequent deprotection. Substantially enhanced antioxidant performance of the novel OVA–EGCG conjugate in liposomes was unveiled with notably reduced curcumin degradation and leakage. The strategy and approaches developed in this study will be valuable to effectively improve the antioxidant activities of protein–catechin grafting conjugates.
研究领域:
食品科学
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化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Qin Wang,Yanjie Li,Zhangyong Tang,Kaifeng Du
The burgeoning requirement for purified biomacromolecules in biopharmaceutical industry has amplified the exigency for advanced chromatographic separation techniques. Herein, macroporous cellulose microspheres (CCMs) with micron-sized pores are produced by a facile regulation via carbon nanotubes (CNTs). In this strategy, the incorporation of CNTs breaks the homogeneous regeneration of the cellulose, thus providing anisotropic phase force to produce macropores. The CCMs have manifested a faster mass transfer rate and more available adsorption sites owing to well-defined macropores (2.69 ± 0.57 μm) and high specific surface area (147.47 m 2 g −1 ). Further, CCMs are functionalized by quaternary ammonium salts (GTAc-CCMs) and utilized as anion adsorbents to adsorb pancreatic kininogenase (PK). The prepared GTAc-CCMs show rapid adsorption kinetics for PK at pH 6.0, reaching 90 % equilibrium within 60 min. Also, GTAc-CCMs for PK exhibit high adsorptive capacity (632.50 mg g −1 ), excellent recyclability (> 80 % removal amount after 10 cycles) and selectivity especially at pH 6.0. Notably, the GTAc-CCMs have been successfully applied in a fixed-bed chromatography process, indicating their potential as an effective chromatographic medium for rapid separation of biomacromolecules.
研究领域:
生物技术
·
材料科学
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制药工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）