BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Qiushi Jiang,Yanjing Li,Minmin Wang,Wen Cao,Xueying Yang,Sihu Zhang,Liejin Guo
This study investigated the mediating effect of Triethanolamine on Fe@C- Rhodobacter sphaeroides hybrid photosynthetic system to achieve efficient biohydrogen production. The biocompatible Fe@C generates excited electrons upon exposure to light, releasing ferrum for nitrogenase synthesis, and regulating the pH of the fermentation environment. Triethanolamine was introduced to optimize the electron transfer chain, thereby improving system stability, prolonging electron lifespan, and facilitating ferrum corrosion. This, in turn, stimulated the lactic acid synthetic metabolic pathway of Rhodobacter sphaeroides , resulting in increased reducing power in the biohybrid system. The ternary coupling system was analyzed through the regulation of concentration, initial pH, and light intensity. The system achieved the highest total H 2 production of 5410.9 mL/L, 1.29 times higher than the control (2360.5 mL/L). This research provides a valuable strategy for constructing ferrum-carbon-based composite-cellular biohybrid systems for photo-fermentation H 2 production.
研究领域:
微生物学
·
可再生能源
·
光合作用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 69
·36603-36614
·
IF 3.708
Hongyun Peng,Lingling Gao,Mengjiao Li,Yibin Shen,Qiongqiu Qian,Xia Li
Bio H2 conversion from wetland lignocellulosic biomass is one of the promising alternatives to fossil fuels. Both Phragmites (sp.) and Thalia dealbata are holocellulose-rich and lignin-rich wetland plant biomasses. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) demonstrated alteration of lignocellulosic structures due to hemicelluloses removal by steam explosion (SE) pretreatment, and further disruption of cellulose crystallinity after treatment with SE followed by [Bmim]Cl (SE-[Bmim]Cl). Thermogravimetry/differential thermogravimetry (TG/DTG) revealed an increase in amorphous cellulose and partial delignification in lignocellulosic structures as a consequence of SE-[Bmim]Cl. The pretreatment of both SE and SE-[Bmim]Cl yielded lignocellulosic substrates with improved properties in terms of their conversion into glucose and bio H2. Five-to-ten folds (by SE) and ten-to-twenty folds (by SE-[Bmim]Cl) more glucose was released from the lignocellulosic substrates of both plants than those of contrast samples. Compared to Phragmites (sp.), the greater destruction in lignocellulosic structure of Thalia dealbata as a result of SE and SE-[Bmim]Cl, increased the accessible surface area and disrupted the cellulose crystallinity much more, thus making it more efficient for bio H2 conversion. The bio H2 of 1.97 ± 0.14 mmol H2 g−1 dry weight (DW) was yielded after sludge anaerobic fermentation of Thalia dealbata treated with SE, and it increased to 4.79 ± 0.86 mmol H2 g−1 DW after SE-[Bmim]Cl treatment. For Phragmites (sp.) it was 1.45 ± 0.42 and 2.75 ± 0.76 mmol H2 g−1 DW after SE and SE-[Bmim]Cl pretreatment, respectively. Therefore, SE-[Bmim]Cl pretreatment can be developed for efficiently enhancing bio H2 conversion from wetland plant Thalia dealbata.
研究领域:
能源生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2024
·
IF 15.8
Wei Zhou,Wen Zhang,Wei Geng,Yaoqi Huang,Tong-Kai Zhang,Zi-Qian Yi,Yang Ge,Yao Huang,Ge Tian,Xiao-Yu Yang
External electric field has the potential to influence metabolic processes such as biological hydrogen production in microorganisms. Based on this concept, we designed and constructed an electroactive hybrid system for microbial biohydrogen production under an electric field comprised of polydopamine (PDA)-modified Escherichia coli (E. coli) and Ni foam (NF). In this system, electrons generated from NF directly migrate into E. coli cells to promote highly efficient biocatalytic hydrogen production. Compared to that generated in the absence of electric field stimulation, biohydrogen production by the PDA-modified E. coli-based system is significantly enhanced. This investigation has demonstrated the mechanism for electron transfer in a biohybrid system and gives insight into precise basis for the enhancement of hydrogen production by using the multifield coupling technology.
研究领域:
微生物学
·
生物工程
·
能源生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Lei Yu,Wei-xin Cheng,Quan Wang
Biohydrogen productions from xylose and glucose under dark condition were enhanced by the presence of natural Fe 3 O 4 . The electron equivalent of H 2 fractions accounted for 4.55 % and 5.69 % of the total given xylose and glucose in the experiments without Fe 3 O 4 , and that were correspondingly increased to 5.14 % and 6.50 % in the experiments with 100 mg/L of Fe 3 O 4 , respectively. Moreover, Fe 3 O 4 increased the total intracellular NAD(H) concentrations by 8.84 % and 8.37 %, and boosted the ratios of NADH/NAD + by 8.33 % and 17.72 % in xylose and glucose fermentation, respectively, comparing to the corresponding control experiments. The formation of electron couples of Fe(III)/Fe(II) during the iron oxide respiration and more generation of active extracellular polymeric substances components were determined as the important reasons for the improved biohydrogen production performance. Thus, a promotion mechanism of the internal “driving forces” from extracellular iron oxide respiration on the biohydrogen production was proposed.
研究领域:
生物技术
·
环境工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2025
·
IF 10
Qian Zhang,Jiawei Hu,Cheng Li
To solve the problem of low bio-hydrogen yield from anaerobic fermentation (AF) of sludge, a novel and efficient method by combining thermal hydrolysis (TH) with calcium hypochlorite (CH) for sludge pretreatment was proposed in this study. Experimental data demonstrated that TH + CH method synergistically promoted the bio-hydrogen production from sludge. The optimal condition of the combined pretreatment was TH at 70 °C plus 0.12 g/g volatile suspended solids (VSS) of CH, and relevant hydrogen yield was 22.52 mL/g VSS. Mechanism study exposed that TH + CH method effectively destructed extracellular polymeric substances (EPS) and sludge cells, causing the promotion of hydrolysis rate and generation of abundant fermentation substrates. In the several biochemical processes of sludge AF, acidogenesis and acetogenesis that responsible for hydrogen generation were not or only slightly suppressed by TH + CH pretreatment, while homoacetogenesis, sulfate-reducing and hydrogenotrophic methanogenesis that can consume hydrogen were severely inhibited under this condition, resulting in hydrogen accumulation. Microbiological analysis demonstrated that the functional bacteria (e.g. Rhodobacter , Proteiniborus and Petrimonas ) related to hydrogen generation were all enriched under TH + CH condition.
研究领域:
环境工程
·
生物能源
·
废弃物管理
·
微生物学
·
生化过程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Hui Xu,Xiaonan Yuan,Yu Rao,Shuna Gao,Jiangna Guo,Feng Yan
Infection and oxidative stress seriously hinder the healing of diabetic wounds, resulting in various serious health and clinical problems. Herein, a sustainable biological hydrogen (H2)-producing hyaluronic acid–based hydrogel patch (HAP-Chl) was constructed by loading an imidazolium-based poly(ionic liquid) (PIL) flocculated live Chlorella as a diabetic wound dressing. The PIL can flocculate Chlorella through electrostatic interactions between PIL and Chlorella to form Chlorella agglomerates, endowing the Chlorella in the central agglomerates with the ability to continuously produce H2 for 24 h under mild conditions. Combining the membrane disruption-related bactericidal mechanism of PIL and the antioxidant properties of the produced H2, HAP-Chl was determined to be antibacterial and antioxidant. In addition to exhibiting biocompatible and nontoxic activities, subsequent Staphylococcus aureus-infected chronic wound studies revealed that HAP-Chl is capable of promoting the healing of chronic wounds by effectively killing bacteria, reducing extensive ROS, relieving inflammation, and promoting the deposition of mature collagen and angiogenesis. This study provides a new strategy for constructing an in situ sustainable H2-producing hydrogel, enabling the formation of novel antibacterial and antioxidant material platforms with potential for wound dressing applications.
研究领域:
材料科学
·
生物医学工程
·
伤口愈合
·
微生物学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）