BIORESOURCE TECHNOLOGY
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2020
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IF 7.539
Ying Zang,Yuan Yang,Yisong Hu,Huu Hao Ngo,Xiaochang C. Wang,Yu-You Li
Pre-concentrated domestic wastewater (PDWW) rich in organic matters can be a suitable substrate for anaerobic digestion (AD) towards holistic resource and bioenergy recovery. Micron zero-valent iron (ZVI) was applied in designed batch experiments during anaerobic treatment of PDWW to verify its roles in performance enhancement and associated mechanisms. In the selected range of food to microorganism (F/M) ratio, 0.5 gCOD/gMLVSS was most appropriate as biomethane production potential (BMP) of 0.275 L CH 4 /gCOD was obtained. The optimal ZVI dosage at fixed F/M of 0.5 was 6 g/L, further enhancing the BMP by 15.2%. Furthermore, ZVI improved the hydrolysis process (producing more soluble organics) and regulated acidification process (affecting volatile fatty acids distribution). No obvious impact on acetoclastic and hydrogenotrophic methanogenesis processes was noted with ZVI addition. ZVI based AD of the PDWW is promising for promoting the practical application of advanced domestic wastewater treatment strategy (pre-concentration plus anaerobic digestion).
研究领域:
环境科学
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微生物学
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Kongyun Zhu,Qiutong Liu,Chao Dang,Aimin Li,Lei Zhang
Integrating hydrothermal carbonization (HTC) and anaerobic digestion for biorefinery-oriented full utilization of wet organic wastes is a promising emerging technology. The objectives of this study were to identify the potential inhibitory substances, evaluate the biomethane potential of mixed and aqueous products and explore process intensifying strategies. The results indicated that the high HTC temperature of 240 °C resulted in a significantly low methane yield of 60 ± 5 mL/g COD and a high Short chain fatty acid (SCFAs) accumulation of 4174 ± 76 mg/L. GC-MS analysis showed that the contents of inhibitory pyrazines, pyridines and ketones in aqueous fraction at 240 °C substantially increased from 13.14%, 0.4%, 0.55% at 180 °C to 23.34%, 2.89%, 5.13%, respectively. When the aqueous products obtained from 240 °C-HTC was supplemented or pretreated by carbonaceous material, the methane yields were greatly improved and increased to 1.3-fold and 1.8-fold, respectively. These finding could provide some valuable technical information for HTC based biorefinery of organic waste.
研究领域:
环境科学
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生物技术
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2024
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IF 9.7
Mingming Yang,Xueli Chen,Lu Ding,Fuchen Wang,Guangsuo Yu
Hydrothermal pretreatment (HTP) is used to increase the biochemical methane potential (BMP) of food waste (FW). The formation of melanoidins will seriously affect the microbial activity and methane production during anaerobic digestion (AD). Based on spectroscopic methods, similarities and heterogeneity of melanoidins from different sources were investigated, and the wide peak band (260–350 nm) in the ultraviolet region and the differences of amide groups of fructose-amino acid system, fructose-casein system and FW system were revealed. Aqueous phase analysis showed that aldehydes, dicarbonyl compounds and diketopiperazine derivatives were the key intermediates in the formation of melanoidins, and the formation of pyrazines reflected additional competitive pathways in the system. Aqueous phase analysis of the fructose-casein system demonstrated that high protein content hindered the formation of pyrazines. Finally, the fluorescence spectra of the fermentation broth before and after AD proved that melanoidins were difficult to be consumed during AD process.
研究领域:
环境科学
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微生物学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2023
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IF 7.7
Zhiwei Dong,Yudong Ding,Fei Chen,Xun Zhu,Hong Wang,Min Cheng,Qiang Liao
The inefficient conversion of carbon dioxide (CO 2 ) to methane often restricts the advancement of CO 2 biomethanation technologies . This study explored the degrading performance, microbial community compositions, and functional gene abundance to examine the impact of zirconium metal-organic frameworks (Zr-MOFs) on the CO 2 biomethanation process. The results showed that the maximum utilization rates of CO 2 and H 2 increased by 2.77 times and 2.36 times after the addition of Zr-MOFs, respectively, and the corresponding conversion rate of CO 2 to methane also increased by 57.53%. Additionally, Zr-MOFs enhanced the COD removal efficiency and hydrolysis of tryptophan-like proteins and humic acid-like substances, resulting in more volatile organic compounds, such as tetradecane, 2-pentanone, and hexadecanoic acid. The analysis of microbial community structure revealed that the relative abundance of Methanothrix and Metanobacterium increased by 24.40% and 36.02% after the addition of Zr-MOFs, respectively. The methane metabolism revealed that Metanobacterium was a member of the same aceticlastic methanogenic pathway as Metanothrix , which encodes acetyl-CoA synthetase to catalyze the synthesis of acetyl-CoA from acetate, and Zr-MOFs also increased the relative abundance of genes that encode the enzyme responsible for converting intermediates to methane. Finally, Zr-MOFs improved the biogenesis and utilization of acetate and hydrogen (H 2 ) by enhancing the activity of enzymes, thereby providing more nutrients for methanogenesis . This study provided thorough explanations for the application and improvement of Zr-MOFs in CO 2 biomethanation technology.
研究领域:
环境生物学
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SUPERCRITICAL FLUIDS
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2023
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IF 3.9
Xiao Feng,Zizhong Zhang,Hongguang Guo,Zhigang Li,Zaixing Huang,Michael Urynowicz
Supercritical CO 2 (ScCO 2 ) extraction could improve the bioavailability of coal for the enhancement of biogenic methane production. Here, the influence of prolonged ScCO 2 extraction on methane yield was investigated since in-situ ScCO 2 injection is time-consuming. The results showed that the enhancement of biomethane production from coal was negatively correlated with extraction time and extraction yield. With the extension of ScCO 2 extraction time from 0.5 day to 28 days, the methane yield of extracted coals increased by 20.1∼111.4% than that of raw coal. ScCO 2 mainly reacted with the organic structures on the surface of coal (such as the C-O and CH 2 -C O), rarely the skeleton structure. In the prolonged extraction, aromatics were first extracted, then oxygen-containing matters and aliphatics which reduces the methane production of extracted coal but increases the methane generation potential of extractable organic matter. Then, a conceptual model of ScCO 2 -MECBM was proposed for enhancing methane recovery in-situ.
研究领域:
环境科学
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化学工程
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能源工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2022
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IF 8.91
Yujia Tang,Shuangshuang Chu,Peixin Su,Ruichao Guo,Yan Duan,Yujie Wang
Anaerobic digestion is widely considered to be a promising technology for waste activated sludge (WAS) treatment, by which sludge stabilization and resource recovery are simultaneously achieved. The poor reaction efficiency however hinders the large-scale applications of WAS anaerobic digestion technology. This study reported an efficient sludge pretreatment method by combining freezing with calcium hypochlorite (Ca(ClO) 2 ) for enhancing the anaerobic digestion efficiency. Experimental data showed that the optimal combination was freezing at −20 °C coupled with 0.075 g/g VSS (volatile suspended solids) Ca(ClO) 2 , by which the maximum biomethane production of 274.4 ± 8.2 mL/g VSS was realized, 1.62 times higher than that of the control. Model-based analysis demonstrated that higher potential and rate for methane production were attained by the combined pretreatment . Mechanism analysis revealed that the extracellular polymeric substances (EPS) and microbial cells were both effectively destructed when treated by combined freezing and Ca(ClO) 2 , and more dissolved organics were generated in consequence. Microbial analysis demonstrated that the co-treated reactor enriched more functional microbes (such as Methanosaeta , Methanosarcina and Candidatus_Methanofastidiosum ) responsible for biomethane generation than that of the control. Furthermore, the number of fecal coliform was largely reduced in co-treated reactor. As the correlation between sludge anaerobic digestion performance and numerous pretreatment parameters was systematically revealed, this study can provide important references for engineers when applying the combined freezing and Ca(ClO) 2 technology in practical engineering.
研究领域:
环境工程
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微生物学
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
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IF 7.963
Zhi-Man Yang,Rong-Bo Guo,Xiao-Huan Dong
Using a batch anaerobic system constructed with 60 mL serum bottles, potential of a composite material with Fe 2 O 3 nanoparticles decorated on carbon nanotubes (CNTs) to enhance biomethane production was investigated. The composites (Fe 2 O 3 @CNTs) with well dispersed Fe 2 O 3 nanoparticles (4.5 nm) were fabricated by a facile thermal decomposition method in a muffle furnace under nitrogen atmosphere. Compared with Fe 2 O 3 , Fe 2 O 3 @CNTs showed a large specific surface area and good electrical conductivity. Supplementation of Fe 2 O 3 @CNTs to the propionate-degrading enrichments enhanced the methane production rate, which was 10.4-fold higher than that in the control experiment without material addition. The addition of Fe 2 O 3 @CNTs also not only showed a clearly electrochemical response to flavin and cytochrome C, but also reduced the electron transfer resistance when compared to the control. Comparative analysis showed that Fe 2 O 3 in Fe 2 O 3 @CNTs played a key role in initiating electrochemical response and triggering rapid methane production, while CNTs functioned as rapid electron conduits to facilitate electron transfer from iron-reducing bacteria (e.g., Acinetobacter , Syntrophomonas , and Geobacter ) to methanogens (e.g. Methanosarcina ).
研究领域:
环境工程
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微生物学
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能源技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2025
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IF 9
Weiqi Zhang,Xin Kong,Linlin Bo,Delin Yin,Zhichun Zhang,Xiaojun Fan,Hongping Chen,Jin Yuan
Zeolitic imidazolate framework-8 (ZIF-8) has been widely employed for carbon dioxide (CO 2 ) and methane (CH 4 ) separation, and CO 2 adsorption and storage owing to its high porosity and tunable pore structure. However, its potential to enhance CO 2 -to-CH 4 conversion by grasping CO 2 in hydrogenotrophic methanogenic systems, thereby increasing CH 4 percentage in biogas, remains unexplored. This study synthesized ZIF-8 material and applied it in a biological biogas upgrading system. The results indicated that the CO 2 -to-CH 4 conversion rate was maximized in the system with 0.75 g/L of ZIF-8 added. Over a single reaction cycle (5 days), the CH 4 proportion increased from 60 % to 97 %. Furthermore, in continuous operation over three reaction cycles, the ultimate CH 4 proportion in this reactor consistently exceeded 95 %, meeting the standards of biomethane. The pathway analysis revealed that the ZIF-8 introducing could increase abundance of hydrogenotrophic methanogens and facilitated the transition of metabolic pathway from acetotrophic to hydrogenotrophic methanogenesis.
研究领域:
环境科学
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微生物学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED ENERGY
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2018
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IF 7.9
Wanwu Li,Habiba Khalid,Zhe Zhu,Ruihong Zhang,Guangqing Liu,Chang Chen,Eva Thorin
Lignocellulosic biomass is the most abundant natural resource with high biomethane potential. However, complex structure of lignocellulosic biomass has hampered the efficient utilization of this bioresource. Previous studies have investigated the overall anaerobic digestion performance of lignocellulosic biomass, but the individual participation of each lignocellulosic component during anaerobic digestion remained unclear. Thus, this study investigated the methane production characteristics of cellulose, hemicellulose, lignin and their mixtures along with the microbial communities involved in anaerobic digestion. The results showed that the biomethane potential of cellulose was higher than that of hemicellulose; however, hemicellulose was hydrolysed more quickly than cellulose, while lignin was very difficult to be digested. The higher concentrations of acetic, n -butyric and n -valeric acids hydrolysed from the hemicellulose resulted in a lower pH and more severe inhibition on methane production than that of cellulose, and the methanogenesis gradually recovered after pH adjustment. The co-digestion of cellulose and hemicellulose increased the methane yield and biodegradability compared to mono-digestions. The addition of lignin to cellulose brought more significant decrease in the methane yield of cellulose than that of hemicellulose. Substrate-related bacteria such as Clostridium sensu stricto , Lutaonella , Cloacibacillus and Christensenella showed higher relative abundance in cellulose digestate, and sugar-fermenting bacteria such as Saccharofermentans , Petrimonas and Levilinea were more rich in the digestate of hemicellulose. Moreover, methanogenic Methanospirillum and Methanothrix likely contributed to the methane production of cellulose, while aciduric methanogens from Methanobrevibacter , Methanomassiliicoccus , Methanobacterium and Methanoculleus contributed to that of hemicellulose. This study provides a deeper understanding of the mechanism in the bioconversion of lignocellulosic biomass during anaerobic digestion.
研究领域:
环境科学
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生物工程
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
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2022
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IF 4.132
Yujia Tang,Jiawei Hu
This work aims to reveal the effect of potassium permanganate (KMnO4) on the sludge anaerobic digestion process, as well as the relevant mechanisms. Experimental data showed that the biomethane production was gradually increased from 159.3 ± 3.0 to 211.5 ± 5.1 mL/g VSS (volatile suspended solids) when the KMnO4 content was increased from 0 to 0.08 g/g VSS, with an increasing rate of 32.8%. A further increase in the KMnO4 dosage however resulted in the decline of the methane yield. First-order kinetic model analysis indicated that higher methane production potentials and hydrolysis rates were achieved in KMnO4-added reactors than in the control. Mechanism analysis demonstrated that KMnO4 not only efficiently disintegrated the sludge flocs, which resulted in the increased contents of dissolved organics, but also enhanced the proportion of biodegradable substances in the sludge liquor. Meanwhile, the biodegradabilities of recalcitrant humus and lignocellulose substances were found to be promoted by KMnO4 treatment as higher methane yields were attained from KMnO4-treated model substrates. 16S rRNA analysis illustrated that the functional microbes participated in anaerobic digestion were largely enriched in the KMnO4-pretreated digestor. Furthermore, efficient inactivation of the fecal coliform was achieved by KMnO4 pretreatment.
研究领域:
环境工程
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废物管理
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生物化学工程
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Yongsen Shi,Boyuan Pang,Yanyan Jia,Haoting Quan,Ning Zhang,Qiujin Deng,Yujian Yan,Fahui Ji,Lianpeng Sun,Hui Lu
Discarded cefradine pellets (DCP) as the hazardous wastes contain lots of bioavailable sucrose. Anaerobic digestion (AD) may be a promising technology for treating DCP, achieving dual goals of waste treatment and resource recovery. However, high concentration of cefradine will inhibit the AD process. This study applied thermo-alkaline pretreatment (TAP) to remove cefradine and improve the AD performance of DCP. Around 95% cefradine could be degraded to different intermediate degradation products (TPs) in TAP at optimal condition, and hydrolysis and hydrogenation were the main degradation pathways. Quantitative structure-activity relationship analysis indicated that the main TPs exhibited lower toxicity than cefradine, and DCP residues after TAP were almost not toxic to E. coli K12 and B. subtilis growth by antibacterial activity analysis. Therefore, TAP promoted the biomethane yield in AD of DCP residues (274.74 mL/g COD), which was 1.91 times that of control group. Besides, compared to control group, final cefradine concentrations in liquids and sludge were significantly decreased in AD system with TAP, lowering environmental risk and indicating stronger prospect for process application. Microbiological analysis revealed that acidogens ( Macellibacteroides , Bacteroides ), syntrophs ( Syntrophobacter , Syntrophorhabdus ), and acetoclastic Methanosaeta were enriched in AD system with TAP, which contributed to improving AD performance of DCP.
研究领域:
环境工程
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废物管理
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生物技术
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY
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2019
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IF 5.537
Shuaishuai Ma,Hongliang Wang,Jingxue Li,Yu Fu,Wanbin Zhu
Lignocellulosic biomass holds great potential in the production of renewable energy such as biomethane via anaerobic digestion . However, the digestion performance of the three main compositions of lignocellulose during biogas production is not well understood so far, and this has greatly hindered the efficient use of biomass by anaerobic fermentation. In this study, the anaerobic digestion performances of glucose, cellulose , hemicellulose , lignin, and their combinations in biomethane production were investigated. The biomethane production potential of cellulose was higher than that of hemicellulose and lignin, and high crystallinity cellulose shows adverse impact on methane production. Hemicellulose had a fast hydrolysis rate and was easier to be digested than cellulose, but it also easily led to excessive acids accumulation. Lignin showed a very limited potential in biomethane production. The co-fermentation of cellulose and hemicellulose could promote cellulose conversion and eliminate the over-acidification of hemicellulose, resulting in efficient and stable biogas production . The co-fermentation of lignin with cellulose and hemicellulose showed an inhibition in biomethane production, and lignin with different structure and sulfur content had different degree of inhibition. This study provides insights into the mechanisms of anaerobic fermentation of lignocellulosic biomass, and paves the way for the better use of biomass.
研究领域:
环境科学
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生物能源
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生物技术
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可再生能源
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）