Nanoscale
·
2020
·Vol. 12
·期 24
·13010-13016
·
IF 6.895
Qiaoling Liu,Xiuyun Niu,Yan Zhang,Ying Zhao,Kaixin Xie,Boru Yang,Qing He,Shiyou Lv,Lin Li
The abnormal concentrations of both biothiols and pH in lysosomes are seriously related to many major diseases, such as Parkinson's and Alzheimer's diseases. Up to now, there are few reports that clearly illustrate the relationship between lysosomal pH and biothiols via fluorescence assay. Herein, novel carbon dots (Scy-CDs) are prepared with good water dispersibility and excellent photostability, and a large Stokes shift of 106 nm is exhibited under an excitation wavelength of 450 nm. The remarkable pH-dependent behavior of Scy-CDs is presented with the fluorescence quenching based on the donor-excited photoinduced electron transfer (d-PET) process. The pKa value is 5.30, which is in good agreement with the range of the normal and abnormal lysosomal pH. Upon the addition of cysteine (Cys) or homocysteine (Hcy), the d-PET process is effectively inhibited with fluorescence recovery totally. The significant co-localization of Scy-CDs with Lyso-Tracker Deep Red in HEp-2 cells and the Pearson correlation coefficient 0.88 strongly suggest that the Scy-CDs can target lysosomal pH and Cys/Hcy in living cells.
研究领域:
细胞生物学
·
医学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Materials
·
2018
·Vol. 11
·期 11
·2232
·
IF 2.467
Jiahui Wang,Hao Zhang,Ruifang Guan
Dual-emission nanosensor for Hg2+detection was prepared by coupling CA-AEAPMS on the surface of RBS-doped modified silica microspheres. The CA-AEAPMS was synthesized by usingN-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS) and citric acid as the main raw material. The obtained nanosensor showed characteristic fluorescence emissions of Rhodamine B (red) and CA-AEAPMS (blue) under a single excitation wavelength (360 nm). Upon binding to Hg2+, only the fluorescence of CA-AEAPMS was quenched, resulting in the ratiometric fluorescence response of the dual-emission silica microspheres. This ratiometric nanosensor exhibited good selectivity to Hg2+over other metal ions, because of the amide groups on the surface of CA-AEAPMS serving as the Hg2+recognition sites. The ratio of F450/F580linearly decreased with the increasing of Hg2+concentration in the range of 0 to 3 × 10−6M, and a detection limit was as low as 97 nM was achieved. Then, the addition of three thiol-containing amino acids (Cys, Hcy, GSH) to the quenched fluorescence solution with Hg2+can restore the fluorescence, and the detection limits of the three biothiols (Cys, Hcy, GSH) are 0.133 μM, 0.086 μM, and 0.123 μM, respectively.Keywords:dual-emission;silica microspheres;fluorescence
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Jia Yu Liang,Lei Han,Shi Gang Liu,Yan Jun Ju,Nian Bing Li,Hong Qun Luo
In this work, sodium salicylate and ethylenediamine (EDA) are used as the precursors to synthesize green fluorescent carbon dots (CDs). The CDs have some attractive properties, including better oxidation resistance, good water solubility, and excellent stability in high ionic strength solutions in a pH range of 6.0–10.0. Compared to other metal ions , only Hg 2+ can quench the fluorescence of CDs, and with the introduction of biothiols, the fluorescence of the CDs/Hg 2+ system can be recovered. Therefore, a turn off/on fluorescent sensor is constructed using CDs as a fluorescent probe , and the sensor is applied to the detection of Hg 2+ and biothiols (glutathione, homocysteine and cysteine). In addition, the fluorescent sensor exhibits excellent selectivity and sensitivity. The linear range of Hg 2+ is 0.05–10 μM with the detection limit of 44 nM. Glutathione, homocysteine, and cysteine have a linear response in the range of 0.5–10 μM with the limit of detection of 80, 76, and 69 nM, respectively. Furthermore, the fluorescence method is successfully used to detect Hg 2+ in actual water samples and biothiols in human plasma.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 32
·期 5
·1921-1930
·
IF 2.525
Gao Xiaoxiao,Zhang Yan,Fu Zheng,Cui Fengling
In this paper, the carbon dots (CDs) with strong blue fluorescence were synthesized through hydrothermal method, which using folic acid, ammonium citrate and ethylenediamine as precursors. The prepared CDs with a high absolute quantum yield of 81.94% and showed excellent stability in high concentration salt solution and different pH conditions. With the addition of Hg 2+ , the signal of CDs was selectively quenched. At the same time, the CDs-Hg 2+ system could be recovered after the introduction of biothiols. Moreover, the fluorescence of CDs showed a good linear relationship with Hg 2+ (1–15 µM), and the detection limit as low as 0.08 µM. In addition, the prepared CDs with low toxicity could be used to detect Hg 2+ in living cells and actual water samples.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 20
·9066-9072
·
IF 3.591
Shaoguang Li,Huilin Liu,Xiaoying Zhang,Jiasong Cai,Kunlong Huang,Bing Chen,Liying Huang,Zhen Lin,Xinhua Lin,Hong Yao
A label-free, rapid, and highly sensitive colorimetric assay for the detection of glutathione (GSH) was developed. o-Phenylenediamine (OPD) can be selectively oxidized by Au3+ in phosphate buffer to produce light yellow 2,3-diaminophenazine. After the addition of GSH, the autocatalytic redox reaction between OPD and Au3+ can be effectively inhibited, resulting in a significant decrease in chroma and absorbance intensity. Reduced absorbance intensity can be used for the quantification of GSH. In addition, this method can achieve the discriminative detection of biothiols, such as cysteine and homocysteine. Based on these findings, a simple colorimetric assay was developed for the detection of GSH in the range of 1 nM to 1 mM with a low detection limit of 0.08 nM. More importantly, this procedure is simple, and the whole assay can be accomplished quickly within 15 min. Additionally, the proposed method was successfully used for the determination of GSH in biological fluids, such as serum samples.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2019
·
IF 4.018
Jing Cao,Xiaoxue Jiang,Nanyan Fu
Due to their highly significant biological activities, in vivo monitoring biothiols, including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), draws great attention. Among them, GSH is the representative nonprotein thiol inside the cell with the high abundance, protecting cells from oxidative stress and toxins. In this work, we developed a fluorescent probe NI for sensitive mapping endogenous biothiol distribution in zebrafish. We used 1,8-naphthalimide scaffold as easy modifiable fluorophore and 2-thiophenecarbonxyl as recognition moiety. After the cleavage of ester bond by GSH addition, NI exhibited strong emission with large Stokes shift (110 nm) and its LOD is 0.120 μM. We also studied the difference of kinetic response of GSH over Cys and Hcy. Finally, the described probe was successfully applied in sensing endogenous biothiol in zebrafish, showing its potential utility in vivo imaging.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Tao Luo,Lingli Bu,Siyu Peng,Yaya Zhang,Zhi Zhou,Guorui Li,Jing Huang
Carbon quantum dots (CQDs), owing to its unique optical properties , have achieved tremendous progress for the detection of different metal ions , organic small molecules, macromolecules , etc. Here we synthesized fluorescent CQDs by a simple one-step microwave-assisted method using 3,4-Dihydroxy- l -phenylalanine (levodopa) as the carbon resource. The CQDs proved to be a multifunctional probe which can be used for detection of various species including copper ion , biothiols and curcumin , via different mechanisms. The selective detection of copper ion was obtained by fluorescence quenching and the mechanism was proved to be static quenching by electrostatic interaction . The fluorescence of levoCQDs-Cu 2+ system can be recovered by biothiols (GSH, Cys and Hcy), implying levoCQDs-Cu 2+ system can also be applied for biothiols detection. The excitation spectrum of levoCQDs had a good overlap with the absorption peak of curcumin, making it as a suitable curcumin probe by fluorescence quenching via inner filter effect (IFE). Furthermore, the levoCQDs can also track the formation of Cu 2+ -Curcumin complexes by restoring the fluorescence of the CQDs in levoCQDs and Cu 2+ -Curcumin system, and this feature may be exploited in the mechanism study of Cu 2+ -Curcumin in the treatment of Alzheimer's disease. The fluorescent levoCQDs were finally used for Cu 2+ and curcumin detection in some real samples including different types of environmental water samples and human serum samples, providing a convenient strategy to monitor Cu 2+ and curcumin in environmental and biological samples.
研究领域:
化学
·
生物学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xiaoyu Wang,Li Qin,Min Zhou,Zhangping Lou,Hui Wei
Nanozymes have emerged as promising alternatives to overcome the high cost and low stability of natural enzymes. Nanozymes with peroxidase-like activities have been studied to construct versatile biosensors by using specific biorecognition ligands (such as enzymes, antibodies, and aptamers) or molecularly imprinted polymers (MIPs). However, the use of bioligands compromises the high stability and low cost promise of nanozymes, while the MIPs may not be applicable to multiplex detection. To address these limitations, here we constructed the nanozyme sensor arrays based on peroxidase-like Pt, Ru, and Ir nanozymes. The cross-reactive nanozyme sensor arrays were successfully used for the detection of biothiols and proteins as well as the discrimination of cancer cells because of the differential nonspecific interactions between the components of the sensor arrays and the analytes. The usefulness of the nanozyme sensor arrays was further validated by the detection of blind unknown samples, where 28 of 30 biothiols and 42 of 45 proteins were correctly identified. Moreover, the practical application of the nanozyme sensor arrays was demonstrated by the successful discrimination of biothiols in serum and proteins in human urine.
研究领域:
分析化学
·
生物医学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
Journal of Analytical Science and Technology
·
2021
·Vol. 12
·期 1
·1-12
·
IF 2.769
Chen Kangni,Li Yanbo,Shang Jinyan,Li Heping
Biothiols play important roles in various physiological and biological processes, which closely related to many diseases. Hydrazine is widely used in the chemical industry, but it is harmful to humans and animals. Therefore, it is very important to develop a fluorescent probe that can simultaneously detect biological thiols and hydrazine. In this work, a new fluorescent probe (2E,4Z)-2-(benzo[d]thiazol-2-yl)-5-chloro-5-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)penta-2,4-dienenitrile (BCD) was synthesized by integrating coumarin and benzothiazole acetonitrile. Featured with four binding sites and different bonding mechanism between probe with biothiols and hydrazine, this probe exhibited fluorescent turn-on for distinguishing Cys, Hcy, GSH and hydrazine with 760-, 8-, 6- and 637-fold fluorescent intensity increase at 502, 479, 476 and 458 nm, respectively, through different excitation wavelengths. Research on the effect of pH on the fluorescent performance of BCD shows that the probe exhibits superior stability in a weakly alkaline to weakly acidic environment, which will facilitate the detection of biological thiols or hydrazine hydrate by BCD. Selectivity studies have shown that the probe has high specificity to biological thiols and hydrazine, which is of great significance to the application of BCD.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Hui Dong,Ke Xu,Zuying Wang,Wentian Chen,Yitong Jiang,Yidan Ye,Zhu Chang,Xu Zhu,Yanli Zhou,Maotian Xu,Yintang Zhang
Reactive sulfur species (RSS) play crucial roles in human physiology, with biothiols like hydrogen sulfide (H 2 S), cysteine (Cys), and glutathione (GSH) being key regulators of oxidative stress and signal transduction. Here, a dual-mode resorufin-based (Re) small-molecule probe (Re-RSS) with electrochemical and fluorescent properties was developed for biothiol detection. Using a ratiometric electrochemical strategy and a “turn-on” fluorescence method, Re-RSS enabled sensitive quantification of H 2 S, Cys, and GSH. The electrochemical approach showed linear ranges of 0–0.3 mM for H 2 S, 0–0.1 mM for Cys, and 0–0.08 mM for GSH. Fluorescence spectrometry revealed linear relationships in the ranges of 1 μM–0.3 mM (H 2 S), 2 μM–0.1 mM (Cys), and 5 μM–0.08 mM (GSH), with detection limits as low as 0.02, 0.01, and 0.08 μM (S/N = 3), respectively. This dual-mode sensor offers three major advantages: (1) dual-readout correction of system/background errors for improved accuracy; (2) independent signal transduction from distinct mechanisms, eliminating cross-interference; (3) negative-potential electrochemical detection free from biological interference. The direct dual-mode quantification highlights its significance and reliability for biothiol analysis in biological systems.
研究领域:
分析化学
·
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 15
·4520-4525
·
IF 4.019
Haiqing Zhang,Yan Xiao,Xiuhua Zhang,Shengfu Wang
Using a simple fluorogenic reaction and the inner filter effect, a label-free ratiometric method for biothiol detection is developed. The reaction between dopamine and resorcinol can generate azamonardine with strong fluorescence. Since the absorption spectrum of azamonardine overlaps with the excitation spectrum of the mercaptopropionic acid-stabilized CdTe quantum dots (MPA-CdTe QDs), the emission of QDs can be quenched via the inner filter effect in the presence of azamonardine. Biothiols can inhibit the above fluorogenic reaction, resulting in the weakening of the fluorescence of azamonardine and the recovery of the emission of MPA-CdTe QDs. The fluorescence intensity ratio of azamonardine to MPA-CdTe QDs can be used to detect biothiols. This approach exhibited a linear trend to the biothiol concentrations in the range of 2 μM to 12 μM, with a limit of detection of 0.6 μM (S/N = 3). This ratiometric analysis strategy was further applied to detect biothiols in human serum samples, showing their great application potentials in real biological samples. The constructed method is simple, easy to prepare, and low-cost.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Shengling Li,Duanlin Cao,Xianjiao Meng,Zhiyong Hu,Zhichun Li,Changchun Yuan,Tao Zhou,Xinghua Han,Wenbing Ma
Given the important role of biothiols in various physiological processes, there is a need to develop novel fluorescent sensors for detecting them. Herein, a novel “on-off-on” fluorescent sensor (E)- N' -((7-(diethylamino)-2-oxo-2 H -chromen-3-yl)methylene)-6-((quinolin-8-yloxy)methyl)picolinohydrazide ( PQC ) was synthesized and its absorbance and fluorescence properties were characterized. The sensor PQC could form a stable complex and showed a significant fluorescence quenching response to Cu 2+ with a quenching efficiency of approximately 100%, and the PQC- Cu 2+ complex showed a fluorescence enhancement response to GSH with a higher recovery rate of above 80% in a CH 3 OH/HEPES (9:1 v/v, pH = 7.23) buffer system. Its detection limits were determined to be 0.17 μM for Cu 2+ and 0.20 μM for GSH, and the binding stoichiometry of PQC- Cu 2+ was determined to be 1: 1 by Job’s plot method. Importantly, the sensor PQC can be used for filter paper strip tests and bioimaging in living cells.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）