DYES AND PIGMENTS
·
2021
·
IF 4.889
Kai Wang,Ya-Xi Ye,Cheng-Yan Jiang,Meng-Ya Guo,Xiang-Yang Zhang,Ai-Qin Jiang,Yu-Shun Yang,Qing-Cai Jiao,Hai-Liang Zhu
Hydrazine is a very important industrial chemical with high toxicity to the human body. In this work, a fluorescent probe HZ , based on the substitution-cyclization-elimination cascade which initiated by hydrazine, was designed and synthesized. HZ had various characteristics such as high selectivity, high sensitivity, fast detection speed, wide application range, and so on. After treating with hydrazine, the fluorescence signal of HZ at 520 nm in aqueous solution was significantly enhanced. Furthermore, HZ had the linear range of 1–10 equivalent for monitoring hydrazine, and the solution showed a colorless to yellow color change. In practical application, the detection limit of HZ for the detection of hydrazine was only 0.75 μM, which could be utilized for trace detection of hydrazine in different water samples. These indicated that HZ had a broad application prospect in the fields of environmental protection and water treatment. This work not only provided a useful instrument for the detection of the ecological environment but also supplied important information for the system optimization of other ecological environment detectors. In addition, HZ could monitor endogenous and exogenous hydrazine in MCF-7 cells successfully, which demonstrated its potential for practical application in complex biological systems.
研究领域:
环境科学
·
分析化学
·
生物学
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Hossain M. Zabed,Suely Akter,Yuehui Tian,Mudasir A. Dar,Junhua Yun,Mei Zhao,Arthur J. Ragauskas,Jia Li,Xianghui Qi
This study evaluated microbial systems and technological approaches to configure the whole slurry co-fermentation process for ethanol biosynthesis from a novel stover system rich in sugars. Two approaches, namely separate and simultaneous hydrolysis and co-fermentation (SHCF and SSCF, respectively), were investigated using Escherichia coli monoculture and E. coli- yeast coculture. The SSCF with E. coli monoculture produced 32.75 g/L ethanol, representing only 44.87% yield, which left 65.13 g/L of total sugars unconverted and exhibited limited xylose consumption. Subsequently, a coculture-based SHCF significantly enhanced sugar consumption, leading to increase in ethanol yield and concentration to 66.94% and 48.86 g/L, respectively. Nevertheless, xylose utilization remained minimal due to the preference for glucose and the inhibitory effects of certain compounds. Thereafter, modification of the medium composition by supplementing betaine and sodium metabisulfite improved ethanol production to 53.18 g/L by reducing the toxic effects of inhibitors. Finally, a dual-phase SSCF (DP-SSCF) was explored by allowing the consumption of sugars from the pretreated slurry in the first phase, followed by concurrent cellulose hydrolysis and utilization of the resulting glucose in the second phase. This strategy increased ethanol titer to 63.14 g/L, with 84.3% yield and 0.88 g/L/h productivity.
研究领域:
生物工程
·
微生物学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2024
·
IF 4.1
Cheng-lu Zhang,Chang Liu,Shi-ru Nie,Yang Zhang,Jing-hao Guo,Cui Liu
Hydrazine is toxic and reductive, and excessive intake will cause irreversible harm to animals and plants. It is necessary to develop an effective method to detect N 2 H 4 . Therefore, in this paper, a novel near-infrared fluorescent probe XCCM was designed and synthesized with xanthene as the fluorophore for the detection of N 2 H 4 . The probe XCCM is very sensitive to N 2 H 4 (LOD = 13.3 nM), fast response (20 s) and has a very large Stokes shift value (231 nm). In addition, it has been successfully applied to detect N 2 H 4 in natural environment (actual water sample, soil and food) and biological system (HepG2 cells).
研究领域:
生物学
·
环境科学
·
分析化学
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DOI
高影响因子
MOLECULES
·
2024
·
IF 4.2
Jiming Zhang,Hui Lin,Chaosong Zheng,Bin Yang,Miao Liang,Yi Lin,Liaoyuan Zhang
2,3-butanediol (2,3-BD) is a versatile bio-based platform chemical. An artificial four-enzyme synthetic biosystem composed of ethanol dehydrogenase, NADH oxidase, formolase and 2,3-butanediol dehydrogenase was designed for upgrading ethanol to 2,3-BD in our previous study. However, a key challenge in developing in vitro enzymatic systems for 2,3-BD synthesis is the relatively sluggish catalytic efficiency of formolase, which catalyzes the rate-limiting step in such systems. Herein, this study reports how engineering the tunnel and substrate binding pocket of FLS improved its catalytic performance. A series of single-point and combinatorial variants were successfully obtained which displayed both higher catalytic efficiency and better substrate tolerance than wild-type FLS. Subsequently, a cell-free biosystem based on the FLS:I28V/L482E enzyme was implemented for upgrading ethanol to 2,3-BD. Ultimately, this system achieved efficient production of 2,3-BD from ethanol by the fed-batch method, reaching a concentration of 1.39 M (124.83 g/L) of the product and providing both excellent productivity and yield values of 5.94 g/L/h and 92.7%, respectively. Taken together, this modified enzymatic catalysis system provides a highly promising alternative approach for sustainable and cost-competitive production of 2,3-BD.
研究领域:
生物化学
·
合成生物学
·
酶学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2017
·Vol. 23
·期 38
·9156-9161
·
IF 5.317
Yanyan Huang,Fang Pu,Jinsong Ren,Xiaogang Qu
Graphical Of mimes and men : Based on the biocatalytic properties of artificial enzymes and the redox characteristic of glutathione, we designed a colorimetric and resettable biological operation to mimic intracellular enzyme-participated redox balance system. By using four artificial enzymes, three simple biological logic gates were fabricated. With the assistance of glutathione, the system could be easily reset without needing a complicated procedure. Owing to the biocatalytic properties of artificial enzymes and the redox characteristic of glutathione, a colorimetric and resettable biological operation was successfully designed to mimic enzyme-participated redox balance in living systems. As one promising candidate of a natural enzyme, artificial enzyme possesses many advantages and has been used in numerous fields. Similar to natural enzymes, communications are also present between different artificial enzymes. With the assistance of four artificial enzymes, three simple biological logic gates were fabricated to help us deepen the understanding of communications between artificial enzymes. In the presence of glutathione, the system could be easily reset without any complicated procedures. We prospect that this work may push forward the exploration of communications between different artificial enzymes and the design of novel artificial enzyme-based biological gates or nanodevices. We hope that our mimic system may help us further understand the functioning of complex biological pathways in biological systems.
研究领域:
生物化学
·
合成生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2021
·Vol. 17
·期 2
·312-321
·
IF 4.099
Yan Jiamin,Lu Yuneng,Xie Shaowen,Tan Haihu,Tan Weilan,Li Na,Xu Lijian,Xu Jianxiong
The establishment of sensing platform for trace analysis of Fe3+ in biological systems is meaningful for health monitoring. Herein, a Fe3+ sensitive fluorescent nanoprobe was constructed based on highly fluorescent N-doped carbon quantum dots (NCQDs) derived from
bamboo stems through a hydrothermal method employing ethylenediamine as the nitrogen dopant. The prepared NCQDs had a uniformly distributed size and their mean size was around 2.43 nm. Abundant functional groups (C=N, N-H, C=O, and carboxyl) anchored on NCQDs demonstrated successful doping
of N in CQDs. The obtained NCQDs possessed a high fluorescence quantum yield of 20.02% and outstanding fluorescence stability over a wide pH range and at high ionic strengths. Moreover, Fe3+ ions presented a specific fluorescent quenching effect to the as-prepared NCQDs. The calibration
curve for fluorescence quenching degree corresponding to Fe3+ concentration showed a linear response in a range of 0.01–10 µM, and detection limit was 0.486 µM, which indicated that the NCQDs had high sensitivity to Fe3+ ions. Ascribed
to these unique properties, the NCQDs were selected as luminescent probes for trace amount of Fe3+ ions in human serum. These results demonstrated their promising use in clinical diagnostics and other biologically relevant studies.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
BioDesign Research
·
2024
·
IF 0
Xinyue Gui,Fei Li,Xinyu Cui,Ranran Wu,Dingyu Liu,Chunling Ma,Lijuan Ma,Huifeng Jiang,Chun You,Zhiguang Zhu
Terpenoids of substantial industrial interest are mainly obtained through direct extraction from plant sources. Recently, microbial cell factories or in vitro enzymatic biosystems have emerged as promising alternatives for terpenoid production. Here, we report a route for the synthesis of α-farnesene based on an in vitro enzyme cascade reaction using methanol as an inexpensive and renewable C1 substrate. Thirteen biocatalytic reactions divided into 2 modules were optimized and coupled to achieve methanol-to-α-farnesene conversion via integration with natural thylakoid membranes as a green energy engine. This in vitro enzymatic biosystem driven by light enabled the production of 1.43 and 2.40 mg liter−1 α-farnesene using methanol and the intermediate glycolaldehyde as substrates, respectively. This work could provide a promising strategy for developing light-powered in vitro biosynthetic platforms to produce more natural compounds synthesized from C1 substrates.
研究领域:
合成生物学
·
生物催化
·
生物能源
·
植物代谢工程
✓ 已验证
DOI
ACS Sensors
·
2021
·
IF 7.711
Fang Fan,Li Zhang,Fangya Mu,Guoyue Shi
A direct detection protocol for the anticancer drug of cisplatin is highly desirable for studying its actions and side effects. In this work, a high quantum yield fluorescent probe with two-photon excitation to detect cisplatin was designed. The probe (RD640-TC) was based on the rhodamine 640 (Rh640) fluorophore, responding to cisplatin with red fluorescence. It showed an excellent linear correlation between the fluorescence response and the concentration of cisplatin over the range of 2–50 μM, suggesting a feasible tool for convenient detection of cisplatin. RD640-TC had high fluorescence quantum yield (Φ = 0.68) and two-photon absorption properties, which made it more favorable to probe cisplatin in biological systems. We exemplified RD640-TC for the detection of cisplatin in cells and zebrafish, providing an accessible tool for in vivo tracking of cisplatin, which has great potential value for studying how cisplatin is processed at cellular level and further for facilitating the investigation into the origin of cisplatin’s toxicity.
研究领域:
药理学
·
生物化学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yantong Liu,Longfei Chen,Le Yu,Jiaomeng Zhu,Fang Wang,Fenghua Jiang,Chengjun Sun,Li Zheng,Yi Yang
The development of engineering microbiology is widely expected to advance carbon neutrality and sustainability in the wastewater industry. Engineering microbiology can deliver desired functions without high treatment costs, complex processes, massive greenhouse gas emissions, and resource waste. Here, we propose a complementary strategy to realize benefited wastewater utilization by configurable spatialized microorganisms-integrated biophotonic systems (CSMBs). The configurable spatialized microorganisms enable enhanced microbial synergy and empower on-demand waste-to-bioenergy conversion through optofluidic-assisted design. The developed CSMBs improve the reaction between light and microorganisms (light harvesting and microorganisms’ photosynthesis) via enhanced natural light photon capture and adjustable optical wavelength. Thanks to these features, in prospective wastewater treatment, the CSMBs breakthrough achieve 89.89% and 91.8% degradation of total nitrogen (TN) and total phosphorus (TP) and 91.98% removal of chemical oxygen demand (COD) within 72 h, respectively. The biomass (the absorbance of 680 nm), lipid content, and chlorophyll-a content of Chlorella in the CSMBs were increased by 56.95%, 62.91%, and 35.41%, respectively, compared to the growth of single Chlorella . The method possesses broad applicability and superior tunability, which can be extended to a wide variety of wastewater treatment and enable more ability in benefited waste utilization.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Yuan Jiao,Yating Meng,Wenjing Lu,Yifang Gao,Yang Liu,Xiaojuan Gong,Yang Liu,Shaomin Shuang,Chuan Dong
A facile strategy for the preparation of nitrogen and phosphorus co-doped carbon dots (N, P-CDs) with long-wavelength emission is attractively proposed in one-pot hydrothermal strategy. The resulting N, P-CDs hold exceptional optical features and display excitation wavelength-independent properties with the emission wavelength at 590 nm, which enable it with the satisfactory relative quantum yield (QY) of 15.6% in long-wavelength region. In addition, the proposed N, P-CDs demonstrates specific selectivity towards ClO − over other competitive reactive oxygen species and exhibits rapid fluorescence response time to ClO − . Moreover, the N, P-CDs exhibits low-cytotoxicity and excellent cell membrane permeability for recognizing ClO − in SMMC-7721 cells, which demonstrates their enormous potential in biological system.
研究领域:
材料科学与工程
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2013
·
IF 3.473
Jinhua Wu,Weizhao Yin,Jingjing Gu,Ping Li,Xiangde Wang,Bo Yang
Batch experiment was conducted to evaluate the capability of a biotic Fe 0 –H 2 O for nitrobenzene (NB) removal from groundwater. In this study, iron dosage was 0.25 g L −1 throughout the whole experiment and the Fe 0 –H 2 O system was amended with 180 mg L −1 VSS of mixed culture. The biotic system was tested at low concentrations (50 mg L −1 as COD) of organic substrates (e.g., ethanol, glucose and sodium acetate) and different concentrations of sulfate, nitrate and dissolved oxygen. The bio-iron system exhibited higher NB removal rate and more AN production. The increasing order of efficiency of tested substrates was: glucose > sodium acetate > ethanol. A complicated enhancement from oxygen was obtained, with 100% of NB removal rate and without any AN accumulation in the solution amended by dissolved oxygen. Sulfate and nitrate functioned as inhibitors since they competed with NB for electron donors (H 2 ), and nitrate showed more significant suppression on NB removal as compared to sulfate. AN elimination occurred during both sulfate-reducing and nitrate-reducing processes and microorganisms got extra reduction capacity from the degradation of AN to reduce nitrate and sulfate, causing the mineralization of NB under anaerobic or anoxic conditions. It suggested that the bio-iron treatment could function as a simplified process for the remediation of groundwater contaminated by combined NB and sulfate, nitrate.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 9
·3625-3637
·
IF 5.56
Wang Juan,Qu Ge,Xie Leipeng,Gao Chao,Jiang Yingying,Zhang Yi-Heng P. Job,Sun Zhoutong,You Chun
Abstract Dihydroxy-acid dehydratase (DHAD) plays an important role in the utilization of glycerol or glucose for the production of value-added chemicals in the in vitro synthetic enzymatic biosystem. The low activity of DHAD in the dehydration of glycerate to pyruvate hampers its applications in biosystems. Protein engineering of a thermophilic DHAD from Sulfolobus solfataricus (SsDHAD) was performed to increase its dehydration activity. A triple mutant (I161M/Y145S/G205K) with a 10-fold higher activity on glycerate dehydration was obtained after three rounds of iterative saturation mutagenesis (ISM) based on computational analysis. The shrunken substrate-binding pocket and newly formed hydrogen bonds were the reason for the activity improvement of the mutant. For the in vitro synthetic enzymatic biosystems of converting glucose or glycerol to L-lactate, the biosystems with the mutant SsDHAD showed 3.32- and 2.34-fold higher reaction rates than the wild type, respectively. This study demonstrates the potential of protein engineering to improve the efficiency of in vitro synthetic enzymatic biosystems by enhancing the enzyme activity of rate-limited enzymes. Key points • A screening method was established for the protein engineering of SsDHAD. • A R3 mutant of SsDHAD with 10-fold higher activity was obtained. • The R3 mutant exhibits higher productivity in the in vitro biosystems. Graphical abstract
研究领域:
酶学
·
合成生物学
·
生物催化
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）