Journal of Fungi
·
2023
·Vol. 9
·期 2
·237
·
IF 4.7
Feifei Wu,Heqin Wang,Qiufeng Chen,Xiao Pang,Hao Jing,Lijun Yin,Xiuqing Zhang
It has been demonstrated that lignin was efficiently degraded byLentinula edodes(L. edodes). However, the process of lignin degradation and utilization byL. edodeshas not been discussed in detail. Therefore, the effects of lignin onL. edodesmycelium growth, chemical compositions, and phenolic profiles were investigated herein. It has been revealed that 0.10% lignin acted as the most effective concentration to accelerate mycelia growth, which yielded the highest biomass of 5.32 ± 0.07 g/L. Furthermore, a 0.10% concentration of lignin promoted the accumulation of phenolic compounds, especially protocatechuic acid, with peak value of 48.5 ± 1.2 μg/g. In contrast, the higher concentration of lignin (0.20%) exerted an inhibitory effect on the growth ofL. edodes. Overall, the application of lignin at the optimal concentration of 0.10% could not only enhance the mycelial growth but also accumulate the phenolic acids and raise the nutritional and medical values ofL. edodes.Keywords:lignin;shiitake mushroom;biomass;phenolic compound
研究领域:
真菌学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Qiulian Liu,Weihua Li,Xiaolong Qiao,Haiguang Zhao
Colloidal carbon dots (C-dots) have excellent optical properties and they have been widely used for various types of applications. Compared to the C-dots prepared using chemicals, the Biomass-derived C-dots have attracted a great of attention because of their environmental friendliness and biocompatibility. However, currently obtained C-dots using biomass have low quantum yield. In this work, we demonstrated an efficient precursor of Magnolia flower for synthesizing high-quantum yield C-dots via hydrothermal approach. High performance liquid chromatography and Bradford assay were used to analyze the compositions of the Magnolia flower, which determine the quality of the C-dots. The typical emission bands of the as-prepared C-dots cover from 400 to 550 nm at different excitation wavelengths. The C-dots synthesized using violet Magnolia flower have the QY as high as 11.9%, which is higher than that of 10.4% (white Magnolia flower), because the violet color gives the higher content of the N. The as-obtained C-dots have excitation-dependent color and high quantum yield, showing a great potential for the use of the anti-counterfeiting system.
研究领域:
材料科学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Chunli Yu,Yao Hu,Yuqin Zhang,Wei Luo,Jing Zhang,Peilun Xu,Jun Qian,Jun Li,Jianfeng Yu,Jin Liu,Wenguang Zhou,Shengxi Shao
In industrial-scale cultivation of microalgae, salinity stress often stimulates high-value metabolites production but decreases biomass yield. In this research, we present an extraordinary response of Arthrospira platensis to salinity stress. Specifically, we observed a significant increase in both biomass production (2.58 g L −1 ) and phycocyanin (PC) content (22.31%), which were enhanced by 1.26-fold and 2.62-fold, respectively, compared to the control, upon exposure to exogenous glycine betaine (GB). The biochemical analysis reveals a significant enhancement in carbonic anhydrase activity and chlorophyll a level, concurrent with reductions in carbohydrate content and reactive oxygen species (ROS) levels. Further, transcriptomic profiling indicates a downregulation of genes associated with the tricarboxylic acid (TCA) cycle and an upregulation of genes linked to nitrogen assimilation, hinting at a rebalanced carbon/nitrogen metabolism favoring PC accumulation. This work thus presents a promising strategy for simultaneous enhancement of biomass production and PC content in A. platensis and expands our understanding of PC biosynthesis and salinity stress responses in A. platensis .
研究领域:
生物技术
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2021
·
IF 4.401
Xinshu Li,Xin Sun,Lin Gao,Juntian Xu,Guang Gao
Pyropia is an important marine crop and periodical dehydration could help kill fouling organisms during cultivation. However, our understanding regarding the effects of periodical dehydration on biomass yield and biochemical composition of Pyropia remains very lacking, particularly for thalli grown at different salinities. In this study, Pyropia yezoensis experiencing periodical dehydration or not was cultured at four levels of salinities (17, 25, 32, 39 psu) for 19 days. The highest growth rate was found at salinity 25 psu and lowest was at salinity 39 psu. Dehydration reduced relative growth rate at each salinity except for the insignificant decrease at 39 psu. Dehydration did not affect the maximal photochemical efficiency (Fv/Fm) and the highest salinity decreased it slightly. Dehydration induced more Chl a and carotenoids and highest Chl a content was found at salinity 39 psu. Dehydration did not affect phycoerythrin or phycocyanin content at salinities 25–39 psu but respectively increased them by 28% and 50% at salinity 17 psu. Dehydration did not affect content of any amino acid while salinity interacted with dehydration on most amino acids. The optimal salinity for the production of total amino acids (AA)，essential AA, umami AA, and sweet AA was 25 psu for the thalli without dehydration; while it shifted to 17 psu for the thalli with dehydration. Dehydration and salinity interacted on content of the fatty acids C12, C18:1(n-9), C18:2(n-6), and C20:2(n-6). For the synthesis of eicosapentaenoic acid (EPA, C20:5(n-3)) and polyunsaturated fatty acids (PUFA) in thalli without dehydration, the optimal salinity was 39 psu, but the effect of salinity disappeared when thalli experienced periodical dehydration. These findings show the interactive effects of periodical dehydration and salinity on biomass yield and biochemical composition in P. yezoensis and provide insights into optimizing conditions for nori cultivation.
研究领域:
海洋生物学
·
藻类学
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2020
·Vol. 25
·期 4
·589-598
·
IF 2.213
He Jinjia,Chen Yi,Dai Luyao,Yao Jiachao,Mei Yu,Hrynsphan Dzmitry,Tatsiana Savitskaya,Chen Jun
Acrylic acid is used in various industrial applications but inflicts harm to human health and causes environmental pollution. A new bacterium, identified as Rhodococcus ruber JJ-3, was isolated, which can degrade high concentrations of acrylic acid rapidly and completely. Experimental results showed that the strain can achieve complete degradation of 1000 mg·L −1 acrylic acid in 11 h under the following conditions: pH 7, temperature 35°C, and inoculation quantity 15%. A high concentration of acrylic acid (2000 mg·L −1 ) can be completely removed in 28 h. According to the Monod model, the maximum specific degradation rate ( v max ) and half saturation rate constant ( K S ) of the strain were 0.85 h −1 and 101.83 mg·L −1 , respectively. The results of carbon balance revealed that 54.6% carbon was assimilated by R. ruber JJ-3 as biomass, and 43.0% carbon was mineralized into CO 2 . Furthermore, glycerol and lactic acid were measured as intermediates, and the possible degradation pathway was proposed during the biodegradation of acrylic acid. These results suggested that R. ruber JJ-3 completely degrades acrylic acid and might have a potential environmental implication in the purification of acrylic acid-contaminated environments.
研究领域:
微生物学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Water Science and Engineering
·
2024
·
IF 3.7
Shan-Shan Gao,Xing Yin,Rui Huang,Jia-Yu Tian
Simultaneous nitrification and denitrification (SND) is an efficient method to remove nitrogen in municipal wastewater treatment. However, low dissolved oxygen (DO) concentrations are generally required, leading to serious membrane fouling in membrane bioreactors (MBRs). This study aimed to clarify the synergistic effect of biomass and DO on nitrogen removal and membrane fouling. To achieve this goal, four submerged MBRs equipped with ceramic membranes were operated with different biomass (mixed liquor suspended solids (MLSS)) concentrations (3 000 mg/L, 5 000 mg/L, 7 500 mg/L, and 12 000 mg/L) under various DO concentrations (2.0 mg/L, 1.0 mg/L, and 0.5 mg/L). As a result, increasing biomass in the MBRs enhanced total nitrogen (TN) removal via SND, and excellent TN removal efficiencies of 60.7% and 75.8% were obtained using the MBR with an MLSS concentration of 12 000 mg/L and DO concentrations of 2.0 mg/L and 1.0 mg/L. However, a further decrease in DO deteriorated TN removal due to the inhibition of nitrification. Moreover, high MLSS concentrations were beneficial to membrane fouling control for ceramic membranes in MBRs. The lowest transmembrane pressure development rate was observed for the MBR with an MLSS concentration of 12 000 mg/L. High biomass offset the adverse effect of DO decrease on membrane fouling to some extent, and improved the stability of the reactor. Therefore, biomass might be an important parameter for membrane fouling reduction in ceramic MBRs. Overall, optimal biomass and DO concentrations for TN removal were identified, providing useful information for the successful operation of MBRs with efficient TN removal and membrane fouling control.
研究领域:
环境工程
·
污水处理
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Yongteng Zhao,Qingqing Li,Dan Chen,Min Yang,Feiyan Huang,Jiani Liu,Xuya Yu,Lei Yu
Dopamine (DA) has attracted attention because of its effects on Haematococcus lacustris biomass, astaxanthin production, and physiological responses. The alga treated with 25 μM DA combined with 1 g L –1 sodium chloride exhibited 7.63 %, 41.25 %, and 52.04 % increases in biomass (1.41 g L –1 ), astaxanthin content (32.37 mg/g), and astaxanthin productivity (3.51 mg L –1 d –1 ) respectively, compared with the salinity stress and high light. Exogenous DA treatment promoted lipid synthesis while reducing carbohydrate and protein contents. Moreover, carotenogenesis and lipogenesis-associated genes were upregulated under DA induction. Inhibition of reactive oxygen species and autophagy, along with mitogen-activated protein kinase activation, promoted astaxanthin accumulation under DA. Furthermore, DA application boosted astaxanthin biosynthesis by regulating the levels of respiratory metabolic intermediates, the γ-aminobutyric acid shunt, and important phytohormones. These findings present a potential and successful biotechnological approach for enhancing biomass and astaxanthin production in H. lacustris under stressful conditions.
研究领域:
藻类生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Guang Gao,Qi Gao,Menglin Bao,Juntian Xu,Xinshu Li
Pyropia yezoensis is an important marine crop in the world. We cultured it under two levels of partial pressure of carbon dioxide (pCO 2 ) (408 (LC), 998 (HC) μatm) and nitrate (30 (LN) and 500 (HN) μmol L −1 ) to investigate the effect of ocean acidification on its growth and food quality under changing nitrogen supply. HC decreased growth rate of P. yezoensis under LN but did not affect it under HN. Phycoerythrin and phycocyanin were enhanced by HC, particularly at HN, which contributed to the darker color. HC stimulated the synthesis of sweat amino acids regardless of nitrate condition and umami amino acid only under LN. HN increased the content of umami amino acids regardless of pCO 2 condition and sweet amino acids only under HC. Our findings indicate that future ocean acidification may reduce biomass yield of P. yezoensis but increase its color and flavor, which was regulated by nitrate availability.
研究领域:
海洋生物学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2020
·
IF 4.402
Cai Kangfeng,Chen Xiaohui,Han Zhigang,Wu Xiaojian,Zhang Shuo,Li Qi,Nazir Muhammad Mudassir,Zhang Guoping,Zeng Fanrong
Drought is a devastating environmental constraint affecting the agronomic production of barley. To facilitate the breeding process, abundant germplasm resources and reliable evaluation systems to identify the true drought-tolerant barley genotypes are needed. In this study, 237 cultivated and 190 wild barley genotypes, originating from 28 countries, were screened for drought tolerance under the conditions of both water deficit and polyethylene glycol (PEG)-simulated drought at seedling stage. Drought stress significantly reduced the plant growth of all barley genotypes, but no significant difference in drought-induced reduction in the performance of barley seedlings was observed under these two drought conditions. Both cultivated and wild barley subspecies displayed considerable genotypic variability in drought tolerance, which underpinned the identification of 18 genotypes contrasting in drought tolerance. A comparative analysis of drought effects on biomass, water relation, photosynthesis, and osmotic adjustment was undertaken using these contrasting barley genotypes, in order to verify the reliability of the screening and to obtain the credible traits as screening criteria of drought tolerance in barley. As expected, the selected drought-tolerant genotypes showed much less reduction in shoot biomass than drought-sensitive ones under water deficit, which was significantly positively correlated with the results of large-scale screening, confirming the reliability of the screening for drought tolerance under two drought conditions in this study. Likewise, the traits of water relation, photosynthetic activity, and osmotic adjustment differed greatly between the contrasting genotypes under water deficit stress, and they were highly correlated to the growth of barley seedlings, suggesting the potential of them to be the selection criteria for drought tolerance. The analysis of the variable importance of these traits in drought tolerance indicated that sap osmolality and relative water content in the youngest fully-expanded leaf are the suitable selection criteria of screening for drought tolerance in barley at seedling stage.
研究领域:
植物学
·
农学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2023
·Vol. 13
·期 1
·83
·
IF 4.5
Qihua Wu,Wenling Zhou,Diwen Chen,Jiang Tian,Junhua Ao
Microplastic pollution in sugarcane areas of China is severe, and reducing the ecological risks is critical. Biochar has been widely used in soil remediation. This study aims to explore the effects and mechanisms of microplastics combined with or without biochar on sugarcane biomass, soil biochemical properties in red soil through a potted experiment. The results show that, compared with control (CK), treatments with microplastics alone reduced the dry biomass of sugarcane, soil pH, and nitrogen (N) and phosphorus (P) contents by an average of 8.8%, 2.1%, 1.1%, and 2.0%, respectively. Interestingly, microplastics combined with biochar could alleviate the negative effects of microplastic accumulation on sugarcane growth and soil quality. There were significant differences in the bacterial community alpha diversity indices and compositions among different treatments. Compared with CK, treatments with microplastics alone obviously decreased the observed operational taxonomic units (OTUs) and the Chao1 and Shannon indices of soil total bacteria (16S rRNA gene-based bacteria) while increasing them inphoD-harboringbacteria. Microplastics combined with biochar treatments significantly increased the abundance ofSubgroup_10for the 16S rRNA gene and treatments with microplastics alone significantly increased the relative abundance ofStreptomycesfor thephoDgene compared to CK. Moreover, compared with microplastics alone, the treatments with microplastics combined with biochar increased the relative abundance ofSubgroup_10,Bacillus,Pseudomonasin soil total bacteria, andAmycolatopsisandBradyrhizobiuminphoD-harboringbacteria, most of which can inhibit harmful bacteria and promote plant growth. Additionally, different treatments also changed the abundance of potential microbial functional genes. Compared to CK, other treatments increased the abundance of aerobic ammonia oxidation and denitrification but decreased the abundance of nitrate respiration and nitrogen respiration; meanwhile, these four functional genes involved in N cycling processes were obviously higher in treatments with microplastics combined with biochar than in treatments with microplastics alone. In conclusion, microplastics combined with biochar could alleviate the negative effects of microplastic accumulation on sugarcane biomass by altering soil nutrients and microbial community structure and function.Keywords:microplastics;biochar;sugarcane biomass;microbial community;red soil
研究领域:
环境科学
·
土壤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Jingjing Hu,Peihua Liu,Qiangqiang Wang,Xuyuan Nie,Jianhua Tan,Junchao Shu,Junjian Mai,Yi Cao,Yongdong Zou,Zebo Huang
Nostoc sphaeroides , originally discovered in selenium-rich fields, is a photosynthetic, nitrogen-fixing cyanobacterium that forms edible spherical macrocolonies. However, prolonged selenium supplementation negatively impacts colony quality in culture. To address this, a two-phase cultivation strategy was developed to spatiotemporally separate biomass accumulation from selenium enrichment, resulting in high-quality selenium-enriched N. sphaeroides colonies. The first phase focused on colony growth in nitrogen-free BG-11 0 medium, while the second phase emphasized selenium enrichment in selenium-supplemented, phosphorus-reduced, and nitrogen-containing BG-11 medium (“selenium enrichment medium”). Scale-up to 300 L confirmed the robustness of this process, achieving desired colony hardness of ∼3 newtons, selenium content of ∼350 μg/g, and yield of ∼0.5 g/L (dry weight). Additionally, polysaccharides containing ∼110 μg/g selenium were isolated from the colonies, exhibiting biochemical properties similar to selenium-free polysaccharides from control colonies. These results provide valuable insight into the potential of N. sphaeroides as a cell factory chassis for producing selenium bioproducts.
研究领域:
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Chemistry Letters
·
2021
·Vol. 19
·期 6
·3973-3979
·
IF 9.027
Yuan Jilin,Xiao Chuanbao,Li Linyang,Zhong Nianbing,Zhong Dengjie,Chang Haixing,He Yuanyuan,Li Tiancong
Phenolic pollution from wastewater induces harmful effects on aquatic ecosystems and human health, calling for advanced remediation methods. Common methods have a single purpose, e.g., contaminant degradation, whereas there is few knowledge on methods combining degradation and algal production. We coupled N-doped TiO 2 -coated photocatalysis with optical fibers and a microalgal biofilm to both remove 4-chlorophenol and produce biomass. We also monitored bacterial population and biomass production in microalgal biofilms. Results show that the biofilm maintained rapid removal of 4-chlorophenol while maintaining the growth of the microalgal biomass, with values of about 78 µM/h for 4-chlorophenol removal, 41 µM/h for dechlorination and 1.8 g/h/m 2 for the rate of biofilm growth. The biofilm became enriched in Salinarimonas and Pseudomonas. Our findings thus reveal a synergy between an optical catalyst and a bioreactor for potential wastewater remediation and microalgal biomass production.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）