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Synthesis, Characterization, and Antiradical Activity of 6-Hydroxygenistein

Shao Jin,Zhao Tong,Ma Hui-Ping,Jia Zheng-Ping,Jing Lin-Lin

A convergent synthesis route of 6-hydroxygenistein (6-OHG) was reported, starting from cheap and readily available biochanin A, via methylation, bromination, methoxylation, and demethylation. The structure of the products was confirmed by MS, IR, 1 H NMR, and 13 C NMR analysis. The antiradical activity of 6-OHG was determined using six different methods, namely, DPPH assay, ABTS assay, nitric oxide assay, superoxide assay, reducing power assay, and phosphomolybdenum assay using ascorbic acid (V C ) as positive control. The results show that 6-OHG possesses similar or greater antiradical activity than V C . In conclusion, 6-OHG can be useful as an antioxidant agent.