JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Dan Huang,Hao-Yun Zhang,Hua-Jun Wang,Hong-Jia Huang,Shuang Liu,Chu-Yi Qiu,Cai-Qin Cheng,Yun Huang,Zhaohe Luo,Wei-Dong Yang,Hong-Ye Li,Xiao-Fei Zheng,Da-Wei Li
Polycyclic aromatic hydrocarbons (PAHs), especially naphthalene (Nap), pose a significant threat to coastal ecosystems and may contribute to the occurrence of harmful algal blooms (HABs). However, the response mechanisms of HAB species to PAH pollution remain unclear. This study investigated the physiological and molecular responses of the HAB species Heterosigma akashiwo to varying Nap concentrations. Low concentrations (1–10 μg/L) promoted growth, while high concentrations (20–1000 μg/L) inhibited growth, causing abnormal cell morphology and oxidative stress. Within 96 h, H. akashiwo removed 82.38 % (10 μg/L) and 88.93 % (100 μg/L) of Nap, mainly through biodegradation and intracellular accumulation. Transcriptomic analysis revealed that H. akashiwo employs a multifaceted adaptive strategy to cope with Nap stress. Key mechanisms include the upregulation of calcium signaling, transcription factors (e.g., zinc finger protein C2H2, myeloblastosis transcription factor, basic leucine zipper transcription factor), heat shock proteins (e.g., HSP40), and fatty acid desaturase (FAD), enhancing stress tolerance through detoxification, antioxidant responses, and membrane integrity. Activation of peroxisomal and oxidative phosphorylation genes indicates improved detoxification and energy metabolism. Differential expression of genes in the PI3K-Akt pathway reveals dose-dependent growth responses, with low concentrations promoting proliferation and high concentrations inhibiting growth. These findings offer insights into H. akashiwo ’s adaptive responses to Nap, aiding ecological risk assessment and bioremediation strategies for PAH pollution in marine environments.
研究领域:
海洋生物学
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环境科学
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生态学
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DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2022
·
IF 4.52
Rui Zhang,Zhigang Zhao,Mingshuai Li,Liang Luo,Shihui Wang,Kun Guo,Wei Xu
Aquatic water with carbonate alkalinity presents a survival challenge to aquatic animals. As an economically important crab, large quantities of Eriocheir sinensis are cultured in carbonate-type saline-alkali ponds, while the toxic effect on E. sinensis from carbonate alkalinity is still unclear. In this study, untargeted liquid chromatography-mass spectrometry metabolomics was performed to investigate the metabolic change caused by culture alkalinity, and confirmed distinct physiological response under gradient alkalinities. There were 39 differential metabolites obtained in the low-alkalinity group (4.35 mmol/L) versus control group, and “arachidonic acid metabolism” was enriched as a core response pathway. 93 differential metabolites were identified in the high-alkalinity group (17.43 mmol/L) versus control group, and a complex response net was manifested through integrated analysis, building by “steroid hormone biosynthesis”, “phenylalanine, tyrosine and tryptophan biosynthesis”, “phosphonate and phosphinate metabolism”, “phenylalanine metabolism”, “mineral absorption”, “purine metabolism” and “carbon metabolism”. This indicated the mobilization of energy reserves and the suppression of protein and amino acid catabolism were manifested in E. sinensis gills to defense high alkalinity stress. In addition, the persistently regulation of key metabolites under various alkalinity, including diuretic compound “spironolactone” and the antiphlogistic compound “LXB 4 ”, suggested anti-inflammatory action and excretion regulation were initiated to defend the stress.
研究领域:
水产养殖学
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环境毒理学
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代谢组学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Pei Fan,Chunhua Liu,Zhen Ke,Wei Zhou,Zhonghua Wu
Ciprofloxacin is ubiquitous and poses a potential threat to aquatic ecosystems. However, the comprehensive effect of prolonged ciprofloxacin exposure on the submerged clonal plant Vallisneria natans (Lour.) Hara remains unknown. Growth and physiological responses in V. natans exposed to ciprofloxacin at concentrations of 0, 0.05, 0.25, 1.25, 2.5, 5 and 10 mg/L were repeatedly evaluated on Days 7, 14, 28, 42 and 56. V. natans maintained good growth properties under 0.05–0.25 mg/L ciprofloxacin treatments, while the inhibition effect on plant growth induced by higher-concentration treatments increased over time. The IC50 values of ciprofloxacin for growth endpoints ranged from 1.6 mg/L to 5.3 mg/L and displayed time-dependent decreases. Pigment contents were significantly stimulated by ciprofloxacin on Day 7 but decreased to varying degrees as the exposure time was extended. Soluble protein and hydrogen peroxide content rose significantly over the first 14 days of treatment with 0.25–10 mg/L ciprofloxacin but decreased under 1.25–10 mg/L ciprofloxacin treatments since Day 28. Antioxidants including superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase and proline functioned well in mitigating oxidative stress under different ciprofloxacin concentrations, lowering the comprehensive toxic effects of ciprofloxacin on V. natans during the period from Day 14 to Day 42, as evidenced by decreased IBR (integrated biomarker response) values. However, the toxic pressure of ciprofloxacin on V. natans peaked on Day 56. These findings suggest that exposure time can influence the responses of V. natans exposed to ciprofloxacin and that IBR can be employed to evaluate the integrated impacts of prolonged ciprofloxacin contamination in aquatic settings.
研究领域:
生态毒理学
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植物生理学
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DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2021
·
IF 6.498
Wanjun Wang,Pan Liao,Guiying Li,Heng Chen,Jingyi Cen,Songhui Lu,Po Keung Wong,Taicheng An
Harmful algal blooms (HABs) caused by Karenia mikimotoi have frequently happened in coastal waters worldwide, causing serious damages to marine ecosystems and economic losses. Photocatalysis has potential to in-situ inhibit algal growth using sustainable sunlight. However, the inactivation and detoxification mechanisms of microalgae in marine environment have not been systematically investigated. In this work, for the first time, visible-light-driven photocatalytic inactivation of K. mikimotoi was attempted using g-C 3 N 4 /TiO 2 immobilized films as a model photocatalyst. The inactivation efficiency could reach 64% within 60 min, evaluated by real-time in vivo chlorophyll-a fluorometric method. The immobilized photocatalyst films also exhibited excellent photo-stability and recyclability . Mechanisms study indicated photo-generated h + and 1 O 2 were the dominant reactive species. Algal cell rupture process was monitored by fluorescent microscope combined with SEM observation, which confirmed the damage of cell membrane followed by the leakage of the intracellular components including the entire cell nucleus. The physiological responses regarding up-regulation of antioxidant enzyme activity (i.e. CAT and SOD), intracellular ROSs level and lipid peroxidation were all observed. Moreover, the intracellular release profile and acute toxicity assessment indicated the toxic K. mikimotoi was successfully detoxified, and the released organic matter had no cytotoxicity. This work not only provides a potential new strategy for in-situ treatment of K. mikimotoi using sunlight at sea environments, but also creates avenue for understanding the inactivation and destruction mechanisms of marine microalgae treated by photocatalysis and the toxicity impacts on the marine environments.
研究领域:
环境科学
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海洋生物学
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生态毒理学
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DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Wantong Tang,Guiyin Wang,Shirong Zhang,Ting Li,Xiaoxun Xu,Ouping Deng,Ling Luo,Yan He,Wei Zhou
Rare earth elements inevitably release into the soil due to their widespread application. However, it is unclear how they affect the soil animals. The study surveyed the growth and physiological responses of earthworm ( Eisenia fetida ) exposed into artificial soils spiked with La , Ce, and their mixture, and actual mine soil collected from an abandoned La–Ce mining area (Mianning, Sichuan). The results showed that the 1000–1200 mg/kg combined exposure in two soils induced significant histopathological and phenotypic changes of earthworms. Concentration significantly affected the superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), and protein of E. fetida and the effects differentiated with the prolonging duration. These indicators were negatively affected under the La stress ≥800 mg/kg (SOD, POD, and protein), the 1200 mg/kg (SOD), Ce stress ≥1000 mg/kg (protein), and the combination ≥800 mg/kg (SOD, POD) and ≥1000 mg/kg (protein). Artificial combination had −15.04% (SOD), 8.87% (POD), 5.64% (MDA), and −8.34% (protein) difference compared with the contamination soil, respectively. Overall, E. fetida respond sensitively under the La and Ce stress, the antioxidant defense system and the lipid peroxidation were stimulated, and the artificial soil might overestimate eco-toxicological effect.
研究领域:
环境科学
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生态毒理学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 28
·期 11
·13955-13969
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IF 3.056
Wang Juncai,Chen Xunfeng,Chu Shaohua,Hayat Kashif,Chi Yaowei,Zhi Yuee,Zhang Dan,Zhou Pei
Solanum nigrum is a well-documented cadmium (Cd) hyperaccumulator; however, its Cd-induced tolerance capability and detoxification mechanism remain elusive. Hence, a short-term hydroponic experiment was performed in a multiplane glasshouse to determine the influence of Cd toxicity on subcellular distribution, chemical forms, and the physiological responses of cell wall towards Cd stress in a 4-week-old plant. The experiment was conducted following completely randomized design (CRD) with five treatments ( n = 4 replicates). The results showed that Cd stress showed dose-dependent response towards growth inhibition. The subcellular distribution of Cd in S. nigrum was in the order of cell wall > soluble fractions > organelles, and Cd was predominantly extracted by 1 M NaCl (29.87~43.66%). The Cd contents in different plant tissues and cell wall components including pectin, hemicellulose 1 (HC1), hemicellulose 2 (HC2), and cellulose were increased with the increase in Cd concentrations; however, the percentage of Cd concentration decreased in pectin and cellulose. Results of the polysaccharide components such as uronic acid, total sugar contents, and pectin methylesterase (PME) activity showed Cd-induced dose-dependent increase relative to exposure Cd stress. The pectin methylesterase (PME) activity was significantly ( p < 0.05) enhanced by 125.78% at 75 μM Cd in root, 105.78% and 73.63% at 100 μM Cd in stem and leaf, respectively. In addition, the esterification, amidation, and pectinase treatment of cell wall and Fourier transform infrared spectroscopy (FTIR) assay exhibited many functional groups that were involved in cell wall retention Cd, especially on carboxyl and hydroxyl groups of cell wall components that indicated that the –OH and –COOH groups of S. nigrum cell wall play a crucial role in Cd fixation. In summary, results of the current study will add a novel insight to understand mobilization/immobilization as well as detoxification mechanism of cadmium in S. nigrum .
研究领域:
植物学
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环境科学
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毒理学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 19
·55079-55091
·
IF 5.8
Liu Huan-Huan,Yang Lei,Li Xiao-Tong,Shi Hui,Guo Lin-Kai,Tu Li-Xin,Wang Jia,Li Yan-Li
Extensive application of nanomaterials enlarges its concentrations in the aquatic environments and poses a threat to algae. This study comprehensively analyzed the physiological and transcriptional responses of Chlorella sp. after being exposed to chromium (III) oxide nanoparticles (nCr 2 O 3 ). The nCr 2 O 3 at 0–100 mg/L presented adverse effects on cell growth (96 h EC 50 = 16.3 mg/L), decreasing the photosynthetic pigment concentrations and photosynthetic activity. Moreover, more extracellular polymeric substances (EPS), especially polysaccharides in soluble EPS, were produced in algae cell, which mitigated the damage of nCr 2 O 3 to cells. However, with the increase of nCr 2 O 3 doses, the EPS protective responses were exhausted, accompanied by toxicity in the form of organelle damage and metabolic disturbance. The enhanced acute toxicity was closely related to the physical contact of nCr 2 O 3 with cells, oxidative stress, and genotoxicity. Firstly, large amounts of nCr 2 O 3 aggregated around and were attached to cells, causing physical damage. Then, the intracellular reactive oxygen species and malondialdehyde levels were significantly increased that led to lipid peroxidation, especially at 50–100 mg/L nCr 2 O 3 . Finally, the transcriptomic analysis further revealed that the transcription of ribosome, glutamine, and thiamine metabolism-related genes were impaired under 20 mg/L nCr 2 O 3 , suggesting nCr 2 O 3 inhibited algal cell growth through metabolism, cell defense, and repair, etc. Graphical Abstract
研究领域:
环境科学
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分子生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2021
·
IF 6.498
Zhuo Jin,Linna Du,Qilu Cheng,Ying Jiang,Cai Hui,Ligen Xu,Yuhua Zhao,Hui Jiang
Microplastics (MPs) and nonylphenol (NP) are typical pollutants that are frequently detected in aquatic environments and can pose a risk to aquatic organisms. However, the responses of algae, the producers in aquatic ecosystems , to MP and NP co-exposure have not been extensively investigated. In this study, polystyrene (PS, 50 mg/L) was selected as a representative MP to evaluate its short-term effects on algae treated with NP (4 mg/L). The results showed that PS mitigated the toxicity of NP to algae after 96 h of exposure, as illustrated by the higher cell densities and pigment concentrations, as well as lower extracellular protein contents and better integrity of intracellular structures, in algae subjected to PS + NP treatment compared with those subjected to NP treatment. Moreover, the upregulated expression of genes involved in photosynthesis and downregulated expression of ribosomal genes as well as genes encoding ATPase and antioxidase, analyzed through RNA-sequencing analysis, further indicated the potential repair and defense mechanisms of PS in NP-treated algae.
研究领域:
环境科学
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水生生态学
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分子生物学
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DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 236
·期 12
·1-15
·
IF 3
Cao Qin,Tan Ai-Juan,Lan Yan,Zou Wen-Bo,Yang Gui-Li
Cadmium (Cd) contamination threatens aquatic ecosystems and human health. Duckweeds are ideal for toxicological studies due to their rapid growth and high metal uptake. While interactions between single duckweed species and Cd has been extensively studied, the impact of duckweed diversity on Cd tolerance remains unknown. This study examines three duckweed species ( Lemna minor , Landoltia punctata , and Spirodela polyrhiza ) under Cd stress, assessing growth, chlorophyll content, Cd accumulation, removal efficiency, and metabolic adaptations in monoculture and mixed-culture systems. The results showed that the growth of duckweeds was inhibited across all cultivation modes under 10 mg L⁻ 1 Cd stress, with growth rates remaining below 0.1 g d⁻ 1 and experiencing more than 80% inhibition. Moreover, chlorophyll content declined with increasing Cd concentration under all cultivation modes, exceeding 64% inhibition under 10 mg L⁻ 1 Cd stress. Cd accumulation peaked in L. minor monoculture at 10 mg L⁻ 1 Cd (1601.33 mg kg⁻ 1 ), with bioenrichment coefficients exceeding 50 in all systems. The combination of S. polyrhiza and L. minor exhibited the highest Cd removal efficiency, achieving 50.60% at a Cd concentration of 1 mg L⁻ 1 . However, the Cd removal rate of all culture modes was below 30% when the Cd concentration increased to 10 mg L⁻ 1 . Elevated antioxidant enzyme activities (POD, CAT) and malondialdehyde (MDA) levels indicated enhanced oxidative stress responses. This study provides support for the application of duckweed polycultures in water contaminated with heavy metals.
研究领域:
植物学
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环境毒理学
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生态毒理学
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植物修复
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产品关联
DOI
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 18
·2918
·
IF 4.1
Guannan Kong,Da Song,Chao Zhang,Xinyao Jia,Yingying Ren,Shuhe Wei,Huiping Dai
Cadmium contamination in soil threatens ecological safety and human health. Phytoremediation has gained attention due to its cost-effectiveness and environmental sustainability. Studies show that plant growth-promoting rhizobacteria can enhance the ability of hyperaccumulator plants to remove heavy metals. This research aimed to isolate and identify plant-growth-promoting rhizobacteria under Cd stress and assess their impact on the growth and Cd accumulation ofSolanum nigrumL. Six bacterial strains were isolated from the rhizosphere ofS. nigrum, all showing high Cd tolerance. Among them, LKT25 exhibited multiple growth-promoting traits, including indole-3-acetic acid production, nitrogen fixation, 1-aminocyclopropane-1-carboxylate deaminase, and siderophore synthesis. Under varying Cd concentrations (5, 25, and 50 mg/kg), theBacillus thuringiensisstrain LKT25 significantly improved Cd removal byS. nigrum. At 5 mg/kg Cd, the removal efficiency reached 45.13%. LKT25 also enhanced plant growth, photosynthesis, and antioxidant activity, contributing to improved Cd remediation. This study provides new microbial resources and technical support for using rhizobacteria in remediating heavy metal-contaminated soils.
研究领域:
环境微生物学
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植物生理学
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土壤修复
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重金属污染治理
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环境生态学
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产品关联
DOI
PubMed
高影响因子
PHYTOPATHOLOGY
·
2020
·Vol. 110
·期 5
·
IF 3.234
Ping Yang,Fang-Jing Li,Shi-Wen Huang,Man Luo,Wei Lin,Gao-Qing Yuan,Qi-Qin Li
Berberine, a botanical drug, has great ability to inhibit the growth of Xanthomonas oryzae pv. oryzae . However, the antibacterial mechanism of berberine against X. oryzae pv. oryzae remains poorly understood. In this study, we investigated the physiological and transcriptional response of X. oryzae pv. oryzae to berberine. When strain X. oryzae pv. oryzae GX13 was treated with berberine (10 µg/ml), the hypersensitive response in tobacco, virulence to rice, pathogen population in the rice xylem, production of extracellular polysaccharide (EPS), and activity of extracellular hydrolases decreased, but the levels of pyruvate and ATP increased. Moreover, biofilm formation was inhibited, and the cell membrane was damaged. Transcriptome sequencing analysis showed downregulated expression of gspD , gspE , and gspF , involved in the type II secretion system (T2SS); hrcC , hrcJ , hrcN , and others, involved in the type III secretion system (T3SS); gumB and gumC , associated with EPS; zapE , ftsQ , and zapA , associated with cell division; lpxH , lpxK , kdtA , and others, associated with the membrane; and pyk , pgk , and mdh , encoding pyruvate kinase, phosphoglycerate kinase, and malate dehydrogenase, respectively. Upregulated expression was observed for nuoA , nuoB , and nuoH , encoding the NADH dehydrogenase complex, and atpF , atpC , and atpB , encoding ATP synthase. An adenylate cyclase ( CyaA ) fusion assay showed that berberine affects type three effector protein secretion via the T3SS and reduces effector translocation in X. oryzae pv. oryzae . It is speculated that the negative growth and virulence phenotypes of berberine-treated X. oryzae pv. oryzae GX13 may involve differentially expressed genes associated with cytoarchitecture and energy metabolism, and these effects on primary cell function may further dampen virulence and result in differential expression of T3SS- and T2SS-related genes.
研究领域:
植物病理学
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微生物学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）