Small
·
2013
·Vol. 10
·期 7
·1379-1386
·
IF 7.823
Yuting Zhang,Wanfu Ma,Dian Li,Meng Yu,Jia Guo,Changchun Wang
Efficient enrichment of specific glycoproteins from complex biological samples is of great importance towards the discovery of disease biomarkers in biological systems. Recently, phenylboronic acid-based functional materials have been widely used for enrichment of glycoproteins. However, such enrichment was mainly carried out under alkaline conditions, which is different to the status of glycoproteins in neutral physiological conditions and may cause some unpredictable degradation. In this study, on-demand neutral enrichment of glycoproteins from crude biological samples is accomplished by utilizing the reversible interaction between the cis -diols of glycoproteins and benzoboroxole-functionalized magnetic composite microspheres (Fe 3 O 4 /PAA-AOPB). The Fe 3 O 4 /PAA-AOPB composite microspheres are deliberately designed and constructed with a high-magnetic-response magnetic supraparticle (MSP) core and a crosslinked poly(acrylic acid) (PAA) shell anchoring abundant benzoboroxole functional groups on the surface. These nanocomposites possessed many merits, such as large enrichment capacity (93.9 mg/g, protein/beads), low non-specific adsorption, quick enrichment process (10 min) and magnetic separation speed (20 s), and high recovery efficiency. Furthermore, the as-prepared Fe 3 O 4 /PAA-AOPB microspheres display high selectivity to glycoproteins even in the E. coli lysate or fetal bovine serum, showing great potential in the identify of low-abundance glycoproteins as biomarkers in real complex biological systems for clinical diagnoses.
研究领域:
生物化学
·
分析化学
·
医学诊断
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DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2014
·
IF 3.377
Peng Wang,Shuang Liu,Hongyun Liu
In the present work, N,N-diethylacrylamide (DEA) and methyl acrylic acid (MAA) monomers were copolymerized into P(DEA-co-MAA) thin films on the electrode surface with a simple one-step polymerization method at ambient temperature and pressure, and the enzyme glucose oxidase (GOD) was entrapped in the films, designed as P(DEA-co-MAA)-GOD. The cyclic voltammetric (CV) response of ferrocene dicarboxylic acid (Fc(COOH)2) at the film electrodes was very sensitive to environmental stimuli, such as temperature, pH, the identity and concentration of anions, and the concentration of CO2 in solution. This multiresponsive CV behavior of the system could be further employed to switch the electrochemical oxidation of glucose catalyzed by GOD entrapped in the films with Fc(COOH)2 as the mediator in solution, demonstrating the amplification effect. The SEM and stereomicroscopy results showed that the multisensitive behaviors of the system were attributed to the structure change of the copolymer films with the stimuli. Specifically, the synergistic effect of temperature and pH was observed, and the hydrogen bonding between PDEA and PMAA components in the copolymer played a key role for this. The present system could be performed under physiological conditions at 37 °C and pH 7.4, which may offer numerous possibilities not only to design new multiswitchable biosensors based on bioelectrocatalysis but also to establish foundations for controlled drug delivery and other medical applications.
研究领域:
生物化学
·
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 33
·5452-5460
·
IF 0
Xinjie Zhang,Chunmei Gao,Shaoyu Lü,Haogang Duan,Nannan Jing,Di Dong,Caifeng Shi,Mingzhu Liu
Optical glucose detection holds considerable promise for continuous in vivo glucose monitoring with wireless transdermal transmission and long-lasting activity. To construct a new class of optical glucose nanobiosensors with high sensitivity and selectivity at physiological conditions, the first generation of fluorescent poly(amido amine) (G1.0 PAMAM), serving as the optical code, was introduced into glucose-sensitive poly(N-isopropylacrylamide-(2-dimethylamino)ethyl methacrylate-3-acrylamidephenylboronic acid) copolymer microgels via a facile method. The fabricated microgels display the ability of adapting to the surrounding medium of different glucose concentrations over a clinically relevant range (0–20 mM) and convert biochemical signals into optical signals. As nanobiosensors, the G1.0 PAMAM functionalized microgels exhibit high selectivity for glucose over various kinds of potential primary interferents, such as lactate, human serum albumin and metal ions, in the physiologically important glucose concentration range. Compared to traditional fluorescent dyes and quantum dots, which are limited by photobleaching and toxicity, this microgel with remarkable anti-photobleaching property and low toxicity makes it possible to be used for long-term continuous glucose monitoring. Through in vivo investigations, it can be observed that G1.0 PAMAM functionalized microgels can achieve wireless transdermal detection, indicating that the fabricated microgels have potential applications as a new generation of nanobiosensors for the highly sensitive and minimally invasive continuous glucose monitoring.
研究领域:
生物医学工程
·
纳米技术
·
生物传感
·
材料科学
·
分析化学
✓ 已验证
DOI
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2025
·Vol. 122
·期 29
·e2510091122
·
IF 9.1
Jia Sun,Yusen Ding,Yicheng Ye,Fei Wang,Hao Tian,Jiamiao Jiang,Huaan Li,Junbin Gao,Haixin Tan,Fei Peng,Jinyao Tang,Yingfeng Tu
Physiological environment with high ionic strength will quench the propulsion of micro/nanomotors (MNMs) by suppressing electric double layers, especially for those motors based on electrolyte diffusiophoresis and electrophoresis. Herein, we demonstrate an efficient, general, and simple strategy to improve the ion tolerance of light-driven titanium dioxide (TiO2) micromotors with amino acid surface modification. Compared to the bare TiO2 counterpart, L-arginine (Arg)-treated TiO2 micromotors display over 200 times higher ion tolerance, which is mainly attributed to the increased surface conductivity. This simple ion tolerance improvement strategy can also be applicable to other motors driven by self-electrophoresis. As TiO2 is an efficient sonosensitizer, we combined the light-guiding ability with ultrasound to generate reactive oxygen species to effectively induce in situ tumor apoptosis. We envision that this simple amino acid surface modification can not only provide a solution for MNMs to tolerate the ionic environment but also open up opportunities for further biomedical and translational research of MNMs.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
生物工程
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2018
·
IF 5.738
Jingsong Yuan,Yi Zhang,Yue Sun,Zhicheng Cai,Lijiang Yang,Hua Lu
Controlling the helix–coil transition of polypeptides under physiological conditions is an attractive way toward smart functional materials. Here, we report the synthesis of a series of tertiary amine-functionalized ethylene glycol (EGx)-linked polypeptide electrolytes with their secondary structures tunable under physiological conditions. The resultant polymers, denoted as P(EGxDMA-Glu) (x = 1, 2, and 3), show excellent aqueous solubility (>20 mg/mL) regardless of their charge states. Unlike poly-l-lysine that can form a helix only at pH above 10, P(EGxDMA-Glu) undergo a pH-dependent helix–coil switch with their transition points within the physiological range (pH ∼5.3–6.5). Meanwhile, P(EGxDMA-Glu) exhibit an unusual salt-induced helical conformation presumably owing to the unique properties of EGx linkers. Together, the current work highlights the importance of fine-tuning the linker chemistry in achieving conformation-switchable polypeptides and represents a facile approach toward stimuli-responsive biopolymers for advanced biological applications.
研究领域:
生物材料
·
高分子化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Yuming Huang,Lin Liu,Hang Yang,Huixuan Yi,Xiangyu Li,Linqian Li,Dawei Lu,Qian Liu,Guibin Jiang
Accurately distinguishing between the endogenous formation and exogenous exposure of Fe-bearing particles (e.g., magnetic Fe particles) within biological organisms is the prerequisite for scientifically evaluating their health risks. However, this remains a challenging task due to lacking the comprehensive understanding of the endogenous formation process of Fe-bearing particles. Here, we report the formation dynamics of Fe-bearing particles under conditions closely resembling actual physiological conditions, and compare the morphological and structural differences between endogenous and exogenous Fe-bearing particles. We find that Fe-bearing particles can indeed form under physiological conditions at 37°C. In this process, phosphate plays a crucial role in the oxidation and mineralization of iron ions. Moreover, endogenously formed Fe-bearing particles typically have a diameter of less than 8 nm, and iron is the only metal element present. Therefore, we propose that Fe-bearing particles found in the body with a diameter larger than 8 nm are mainly derived from exogenous exposure. For Fe-bearing particles smaller than 8 nm, it is necessary to combine associated elements and crystal structure characteristics to distinguish between endogenous and exogenous sources. This study provides direct evidence from endogenous metabolism for tracing Fe-bearing particles, especially magnetic iron particles, within the human body.
研究领域:
材料科学
·
生物医学工程
·
环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yulin Liu,Shun Hu,Guoli Zhang,Qiuhua Wu,Guolin Zhang,Xue Liu
Protein types and concentrations in human body fluid environment have a direct correlation to health conditions, and can be used for primary screening and diagnostics of a great variety of diseases. Herein, we synthesized a series of fluorescent carbon nanodots (CDs) with various ratios of β-cyclodextrin doping, which can recognize proteins and adjust self-emission by combining both the cavity recognition effect of cyclodextrin and noncovalent bonding effect of carboxyl, amino and hydroxyl groups . The difference in β-cyclodextrin contents of various CDs will result in a differential response to various proteins, based on which a sensor array by four CDs was constructed to detect and discriminate six proteins with various subunit numbers. In order to improve the discriminative effect of the sensor array, CD-sensitive metal ions (Cu 2+ and Fe 3+ ) were introduced as sensing media to increase the action sites between the CDs and proteins, and these proteins were well discriminated by the [email protected] ion sensor array with a detection limit of 50 μM. The sensor array could work in physiological conditions (such as urine and serum) and realize multiple proteins detection. Combining with the good repeatable detection results and higher sensitivity, the sensor array based on the CDs and metal ions provides an efficient and accurate method to detect proteins, which is important for disease diagnosis through assessing protein levels.
研究领域:
分析化学
·
生物技术
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Peng Zhang,Xinjie Guo,Yuzhe Xiao,Qian Zhang,Caifeng Ding
Accurate detection of human serum albumin (HSA) in biological samples is quite meaningful for early disease diagnosis and treatment. Herein, a novel fluorescent probe 1-ethyl-4-[2-[4-(diethylamino)-2-hydroxyphenyl]ethenyl]]-pyridinium salt (DEHP) was developed for HSA determination. The inherent fluorescence of DEHP is essentially negligible at physiological conditions assigned to the well-developed twisted intramolecular charge transfer (TICT) protocol. An intriguing fluorescence light up is triggered as the addition of HSA, on account of the inhibited TICT procedure when DEHP enters the hydrophobic cavity of protein HSA. This combination leads to a turn on fluorescent response for HSA with a detection limit of 4.8 nM. After an overall investigation, it has been proved that the strong binding between DEHP and HSA is specific-site-related. In additional, the probe implies a great potential to assist clinical diagnosis due to the usage in actual serum detection. Cell imaging also shows that the probe is expected to monitor HSA production process at cell level.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
临床诊断
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale
·
2025
·
IF 5.1
Jiaxuan Wang,Shuang Feng,Tiwei He,Zenan Li,Tianyu Shi,Tao Cheng,Hui Huang,Yang Liu,Zhenhui Kang
Hydrogen (H2) has emerged as a therapeutic agent with potent antioxidative and anti-inflammatory effects, offering promising applications in the treatment of neurological and cardiovascular diseases. However, the development of controllable, clean, and in situ H2 generation strategies remains a significant challenge in biological systems. Herein, we report a metal-free carbon dot (CD) based photocatalyst synthesized via thermal polymerization of uracil, is capable of efficient and biocompatible photocatalytic H2 evolution. The photocatalyst exhibits a well-defined structure composed of interconnected C–N five-membered rings, where nitrogen atoms serve as active sites for proton adsorption and reduction. Under simulated physiological conditions, the photocatalyst enables H2 production without water oxidation or oxidative side reactions, thereby maintaining the ion concentration and pH stability. A H2 evolution rate of 73.87 μmol h−1 g−1 is achieved, and tunable H2 release is realized by varying the catalyst dosage, illumination time, and light intensity. Importantly, cytocompatibility tests reveal an enhanced cell viability of up to 165.6%, demonstrating the excellent biocompatibility and the potential of this system as a bio-green platform for therapeutic H2 delivery.
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Ruiyang Xiao,Yifu Fu,Lu Bai,Chu Chu,Junye Ma,Zongsu Wei,Richard Spinney,Dionysios D. Dionysiou,Jiaxi Pu
Ascorbate (Asc) has long been known to be an antioxidant against reactive oxygen species (ROS) for cytotoxicity and human diseases. However, its reported reactivity with superoxide radical ( O 2 • − ), an important ROS involved in aerobic metabolism system, exhibits great discrepancy; its mechanistic mode of actions on the molecular level is also largely debatable. Here, we investigated the reaction of Asc and O 2 • − by a combination of experimental and theoretical approaches. The rate constant measured by a competitive technique method was determined to be 3.3 × 10 4 M −1 s −1 under physiological condition. Mechanistically, our theoretical result indicated that O 2 • − abstracts a hydrogen atom on the enolic hydroxyl group forming ascorbyl radical (Asc • ), which is unreactive with O 2 • − or Asc • itself. However, with different protonation state of Asc • ( i.e., AscH • ) in acidic environment, its reactivity was demonstrated to be significantly altered. AscH • can react with O 2 • − via hydrogen atom abstraction and single electron transfer and/or undergo self−disproportionation. These complementary results provided fundamental principles for the ecotoxicological function and environmental potentials of Asc against ROS.
研究领域:
化学
·
生物化学
·
环境科学
·
分子生物学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Jinxin Li,Hao Zhang,Xijuan Yang,Ling Zhu,Gangcheng Wu,Xiguang Qi,Hui Zhang
The carbonyl trapping activity of bound-polyphenol rich insoluble dietary fiber (BP-IDF) from different whole grains and underlying mechanism of these BP-IDF actions were studied under simulated physiological conditions. We found that the black highland barley BP-IDF exhibited the most pronounced effect in scavenging carbonyls by trapping 88.7%, 72.2%, 95.7%, and 31.4% for methylglyoxal, glyoxal, acrolein, and malondialdehyde within 24 h, respectively. After vitro gastrointestinal digestion, the black highland barley BP-IDF still retained considerable trapping activity for carbonyls. The carbonyl scavenging capacity was reduced by up to 93% after removing bound polyphenols from the black highland barley BP-IDF, which was consistent with the reduction in its total phenolic content. Moreover, the formation of adducts between reactive carbonyl species (RCS) and polyphenols bound to insoluble dietary fiber (IDF) was also detected. Overall, these findings confirmed that IDF-bound polyphenols were still active to trap RCS, indicating the potential benefits of BP-IDF from whole grains as functional ingredients to limit carbonyl stress across the gastrointestinal tract.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2018
·
IF 8.806
Shan Yu,Yiyuan Duan,Xingang Zuo,Xinyi Chen,Zhengwei Mao,Changyou Gao
Cell migration plays an important role in many physiological and biological processes, which is influenced by both physicochemical properties of surrounding matrix and signal gradient generated by neighboring/remote cells. Here we aim to develop a co-culture system of immune cells and smooth muscle cells (SMCs) based on the combination of Transwell and cell-responsive hydrogels. This model can be used to study the cell invasion into hydrogels in dynamic physiological conditions , with better mimicking of the in vivo microenvironment . Methacrylic anhydride-modified hyaluronic acid (MA-HA) macromolecules were crosslinked by matrix metalloproteinases (MMPs) sensitive peptides (MMP SP) to fabricate a cell-degradable hydrogel mimicking dynamic extracellular matrix (ECM). The migration of SMCs into the MMP-sensitive hydrogel was investigated under the existence of U937 cells, a type of macrophage-like cells. The invasion distance of SMCs in the MMP-sensitive hydrogels was much longer than that in the MMP-insensitive ones both in vitro and in vivo . The impact of hydrogel degradability and inductive signal gradient generated by U937 cells on cell invasion was compared, revealing that the degradability plays a major role in regulating cell invasion into the 3D hydrogels. Further mechanism investigation revealed that the expressions of cell migration-related genes and proteins were significantly up-regulated in the MMP-sensitive hydrogels compared to those in the MMP-insensitive hydrogels.
研究领域:
生物医学工程
·
细胞生物学
·
组织工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）