Advanced Materials Interfaces
·
2021
·Vol. 8
·期 23
·2101605
·
IF 6.147
Shuai Chen,Yong Yan,Yue Yu,Zhenfeng Wang,Xiaojun Zhu,Liping Sun,Chao Li,Feng Wang
Pathogenic bacteria and biofilm formation have become the urgent threats to global public health. Herein, metal-epigallocatechin gallate (EGCG) network with improved bactericidal effect and pathogenic biofilm eradication ability via trivalent cationic Fe 3+ catalyzed hydrogen peroxide (H 2 O 2 ) decomposition is developed. EGCG, the predominant catechin from tea, is predicted to be a good chelator for eight metal ions (i.e., Al 3+ , Cr 3+ , Cu 2+ , Co 2+ , Zn 2+ , Mn 2+ , Ni 2+ , Fe 3+ ). 3,3',5,5'-tetramethylbenzidine oxidation experiment using UV–vis spectrometer demonstrated that Zn 2+ , Mn 2+ , Ni 2+ are the potential candidates to accelerate the generation of H 2 O 2 by EGCG. Compared with the other two metal-EGCG network (Zn 2+ -EGCG or Mn 2+ -EGCG), only Ni 2+ -EGCG network has better bactericidal property and plays a synergistic role in the network system. Furthermore, by introducing Fe 3+ to Ni 2+ -EGCG network, the complex exhibits the best bactericidal property for gram-negative bacteria Escherichia coli ( E. coli ) and Pseudomonas aeruginosa (PAO1), which can be explained by the rapid H 2 O 2 decomposition and associated production of hydroxyl radicals. With SYTO 9/PI staining assay, Fe 3+ as a catalytic mediator in Ni 2+ -EGCG network shows the most significant bactericidal effect and pathogenic biofilm eradication ability. According to the above analysis, Ni 2+ -EGCG network presents a promising nanoplatform as an alternative to antibiotics for eradication of pathogenic bacteria and biofilms.
研究领域:
微生物学
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材料科学
·
医学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Lu Shi,Feifei Cao,Limin Zhang,Yang Tian
The development of switches responding to specific pH changes was particularly useful in wide application fields. Owing to flexible switches simulated by pH, i-motif DNAs are widely used as a pH sensor. But its character of structure transition strongly dependent on acidic pH severely hampers the application of i-motif DNA in physiological media. Herein, we report the stable i-motif structure formed at a physiological pH triggered by spatial confinement of silica nanochannels. Three classic DNA chains containing 21-mer i-motif domain base-pairs and a single-stranded multiply (T)n spacer, 5′-COOH-(T)n-CCCTAACCCTAACCCTAACCC-3′, were employed to evaluate the enhanced stability of i-motif structure. Compared to their free states in a dilute solution, the transition pH of all i-motif DNAs decorated in nanochannels remarkably shifts toward a neutral pH. Moreover, the transition midpoint can be tuned sensitively over the physiologically relevant pH range through slightly varying the length of T base spacer. Density functional theory (DFT) calculations validate that the increased proton density in a nanochannel triggers the formation of an i-motif structure under a neutral pH. Finally, this i-motif DNA based nanochannels electrode was successfully employed to monitor pH in brain microdialysates followed by cerebral ischemia. The present approach is not limited by fundamental investigation for DNA conformation but may extend toward the manipulation of i-motif based structures for artificial molecular machines and signaling systems.
研究领域:
生物化学
·
分子生物学
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医学检测
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Xiulian Yin,Peng Liu,Xuechao Xu,Jianming Pan,Xin Li,Xiangheng Niu
In recent years, a growing number of nanosized materials that can mimic the catalytic characteristics of some natural enzymes have experienced a period of rapid development. Among these nanomaterials defined as ‘nanozymes’, peroxidase-mimicking materials draw intensive interest because of their potential applications in various fields, including biosensing, medicine, and environmental engineering. Nonetheless, most of currently explored peroxidase nanozymes exhibit favorable activity only in acidic environments, which greatly restricts their use especially in biochemical analysis. Developing peroxidase mimics with good catalytic performance in a broad pH range is highly desired and still challenging. Herein, we report a vitriolization strategy to broaden the catalytic pH scope of peroxidase-like CoFe 2 O 4 . The vitriolization process endows the nanomaterial with sustainable acidity on its surface. As a result, the obtained peroxidase nanozyme (SO 4 2− /CoFe 2 O 4 ) exhibits good catalytic activity in neutral or even weak alkaline solutions. This provides us a one-step assay for colorimetric glucose detection in one pot at physiological pH via combining the proposed peroxidase mimic with glucose oxidase, which shows advantages of easier operation and faster detection while offering comparable analytical performance.
研究领域:
生物化学
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医学检测
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纳米材料科学
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DOI
高影响因子
TALANTA
·
2014
·
IF 3.511
Qingqing Zhang,Ming Zhou
A ratiometric pH probe composed of a fluorescein moiety and an ionic near-infrared-emitting phosphorescent cyclometalated iridium(III) complex bis(6-(benzo[b]thien-2-yl)phenanthridinato)(4-(3-carboxypropyl)-4′-methyl-2,2′-bipyridine)iridium(III) was synthesized. With good cell permeability, the probe demonstrated a linear ratiometric response to the pH variation in the physiological range in HeLa cell assay.
研究领域:
生物化学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Wei Chen,Zhiyang Guo,Hao Yu,Qingyun Liu,Min Fu
Glycoproteins play vital roles in living organisms and often serve as biomarkers for some disease. However, due to the low content of glycoprotein in biological fluids, selective detection of glycoproteins is still a challenging issue that needs to be addressed. In this study, molecularly imprinted colloidal array with multi-boronic acid sites for glycoprotein detection under physiological pH was proposed. Monodispersed glycoprotein imprinted particles (SiO 2 @PEI/MIPs) was first prepared based on surface imprinting strategy using horseradish peroxidase (HRP) as template, and polyethyleneimine (PEI) was used to increase the number of boronic acid groups. The binding experiment indicated that the SiO 2 @PEI/MIPs hold satisfactory adsorption capacity (1.41 μmol/g), rapid adsorption rate (40 min) and preferable selectivity toward HRP. Then the SiO 2 @PEI/MIPs was assembled into close-packed colloidal array to construct a label free optical sensor (denoted as GICA). Benefiting from the high ordered photonic crystal structure, binding of HRP onto the GICA could be directly readout from the changes in structure color and diffracted wavelength. The structure color of the GICA changed from bright blue to yellow with the diffraction wavelength red shifted 59 nm when the HRP concentration increased from 2.5 to 15 μmol/L. Importantly, the GICA was capable of detecting HRP from human serum samples. All those results indicated the potential of the GICA for naked-eye detection of glycoprotein.
研究领域:
生物化学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2014
·Vol. 21
·期 7
·1-8
·
IF 1.897
Wu Wei,Wang Weigang,Li Shuai,Wang Jiantao,Zhang Qiujing,Li Xiuhua,Luo Xianglin,Li Jianshu
In order to develop polymeric self-assembly with an intelligent morphological transition property under appropriate stimuli, a series of pH-sensitive amphiphilic block copolymers have been prepared via atom transfer radical polymerization (ATRP). Due to the delicately designed macromolecular structure, poly(ethylene glycol) 44 - b -poly(2-diisopropylaminoethyl methacrylate) 15 (PEG 44 - b -PDPA 15 ) could form self-assemblies with a controllable morphological transition in response to the physiological pH changes. As demonstrated by the results of dynamic light scattering (DLS) records and transmission electron microscopy (TEM) micrographs, PEG 44 - b -PDPA 15 could self-assemble into toruloid aggregates arranged by several single micelles at pH 7.4, but the morphology changed into uniform single micelles at pH 6.5. With a further decrease of pH value to 5.5, PEG- b -PDPA became double hydrophilic and could not self-assemble into any nanostructure. Therefore, this block copolymer provides a feasible approach to construct a nanoscale smart self-assembly with adjustable morphologies, which exhibits its potential for biomedical applications with specific physiological pH stimuli, such as intracellular delivery and tumor therapy.
研究领域:
生物材料学
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高分子化学
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生物医药工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Hengjia Zhu,Hang Yao,Kexu Xia,Jinxin Liu,Xiulian Yin,Wenli Zhang,Jianming Pan
Boronate-functionalized surface imprinted nanoparticles that function under physiological pH would be highly desirable for specific enrichment of target glycoproteins. In this work, magnetic molecularly imprinted polymers integrated with low pKa teamed boronate affinity (Fe 3 O 4 @PGMA-TBA/MIPs) were fabricated for selective separation of glycoprotein at pH = 7.4. The teamed boronate affinity (TBA) was formed by boron-nitrogen (B-N) coordination between 1,6-hexamethylenediamine and 3-aminophenylboronic acid, and then was fixed on the surface of magnetic poly(glycidyl methacrylate) (Fe 3 O 4 @PGMA) through ring-opening reaction. After immobilizing the template glycoproteins (ovalbumin, OVA), surface imprinting layer was deposited onto Fe 3 O 4 @PGMA-TBA surface via redox polymerization of aniline, and Fe 3 O 4 @PGMA-TBA/MIPs with obvious core–shell-shell structure were prepared by removing template. Fe 3 O 4 @PGMA-TBA/MIPs were demonstrated with an imprinted polymer film (10–20 nm) and exhibited superparamagnetic property ( M s = 32 emu g −1 ) and magnetic stability after multiple regenerations. Besides, the results of 11 B MAS NMR spectrum and zeta potentials confirmed the presence of TBA resulting from B-N coordination. Additionally, taking advantage of surface imprinting and TBA, as-prepared Fe 3 O 4 @PGMA-TBA/MIPs posed high binding capacity (190.7 mg g −1 ) and fast capture kinetics (50 min) toward OVA under physiological pH. More importantly, because of the TBA was electroneutral at pH = 7.4, Fe 3 O 4 @PGMA-TBA/MIPs not only exhibited superior specific recognition toward OVA (imprinting factor IF = 7.51), but also maintained the activity of OVA from practical samples analysis. Therefore, this work opened up a universal route for developing intelligent controllability molecular imprinting materials for the specific separation of glycoproteins in biomedical filed under physiological pH.
研究领域:
生物化学
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材料科学
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医学
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产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Muhammad Mujahid Ali,Dilshad Hussain,Yan Tang,Xuechun Sun,Zhengchao Shen,Fengxia Zhang,Zhenxia Du
Boronic acid-based affinity materials have gained tremendous attention for the selective separation and recognition of cis-diol containing biomolecules. But often, these boronate affinity materials are stuck to some serious issues like high binding pH and weak affinity, especially in the case of glycoproteins . Here in this study, we used 5-boronoisophthlic acid as a novel affinity ligand for the selective capture and release of glycoproteins. The pKa value of 5-boronoisophthalic acid is investigated to be 7.8 which is just closed to physiological pH and is ideally suitable for the fast binding and elution kinetics of glycoproteins to avoid their degradation and deactivation. The affinity ligand is attached to the surface of polymer support using branched polyethyleneimine (PEI) which enhances the binding strength as it has multiple amine groups available for the attachment of 5-boronoisophthalic for synergistic interactions. The resulting affinity material is characterized and packed in a micropipette-tip using hydrophilic melamine foam as a frit to make the separation process smooth, simple, reliable, and robust. This boronic acid-based affinity tip exhibits binding constants for model glycoproteins in the range of 10 −6 –10 −7 M, binding capacities in the range of 0.662 μM/g, and selectivity up to 1:1000 (HRP to BSA) under optimized extraction conditions. Finally, the boronic-based affinity tip is successfully applied to selectively capture the glycoproteins from the human milk sample, especially lactoferrin which is highly important in dairy manufacture.
研究领域:
生物化学
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分离科学
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蛋白质组学
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DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 12
·2049
·
IF 5.719
Tianyu Gao,Jinwei Lin,Leilei Xu,Jianguo Guan
In living bodies, pH values, which are precisely regulated and closely associated with diseased cells, can act as an efficient biologically intrinsic indicator for future intelligent biomedicine microsystems. In this work, we have developed flask-like carbonaceous nanomotors (FCNMs), via loading Fe3O4nanoparticles (NPs) into a cavity, which exhibit a self-adaptive feature to a specific physiological pH by virtue of the pH-dependent dual enzyme-like activities of Fe3O4NPs. Specifically, the peroxidase-like activity of Fe3O4NPs in an acidic pH range, and the catalase-like activity in a near neutral and alkaline pH range, determine the products in the motion system (•OH, ions and O2), whose diffusions from the inner to the outside of the flask result in fluid movement providing the driving force for the movement of the FCNMs. Correspondingly, changes of the product concentrations and species in the physiological pH range (4.4–7.4) result, firstly, in velocity decrease and, then, with increase in pH, increase of the FCNMs occurs. Thanks to the non-linear velocity responsiveness, the FCNMs show intriguing pH taxis towards 6.8 (generally corresponding to the physiological pH in tumor microenvironments), where a maximum velocity appears. Furthermore, the superparamagnetic feature of the Fe3O4NPs simultaneously endows the FCNMs with the abilities to be magnetic-oriented and easily separated. This work could significantly increase the possibility of nanomotors for targeted therapy of tumors and next-generation biotechnological applications.Keywords:nanomotors;pH responsiveness;Fe3O4nanoparticles;dual enzyme-like activities;physiological pH chemotaxis
研究领域:
生物医学工程
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jingjing Kuang,Shengli Ruan,Yangkun Sun,Zeyu Wu,Jiayao Xu,Ting Zhang,Xiaofan You,Shiman Yang,Min Zhang,Hongyang Zhang,Ping Hu
Exosomes are extracellular vesicles that inherit molecular markers from their parent cells for the transfer and delivery of membrane and cytosolic molecules between cells. Exosome detection and protein profiling provide noninvasive access to disease diagnosis and treatment. The nanozyme designed in this paper contains polydopamine-functionalization of graphene oxide (rGO@PDA) units that can adsorb ssDNA aptamers and CeO 2 metal oxide nanosheet units with peroxidase activity. As auxiliary regulator, adenosine triphosphate (ATP) enhances peroxidase activity of rGO@PDA@CeO 2 under a wide range of pH (4–7.4), which breaks through the limitation that traditional nanozymes can only exhibit peroxidase activity under acidic conditions. The ssDNA aptamers, which are recognition probes of exosome, can be adsorbed on the surface of rGO@PDA by aromatic stacking, competing with ATP molecules. Thus, the ssDNA aptamer acts as the switch for colorimetric sensor to flexibly regulate the peroxidase activity of the rGO@PDA@CeO 2 under physiological conditions , and realizes sensitive detection of exosomes. This assay achieves a detection linear range of 0.46 × 10 7 –10 8 particle/mL and a limit down to 3.81 × 10 5 particles/mL. In addition, this aptasensor sensitively profiles six exosomal protein across five cell types and clinical samples, suggesting their potential in clinic diagnosis.
研究领域:
生物医学工程
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分子生物学
·
纳米技术
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产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2025
·
IF 5.5
Jun Liu,Xiangyun Lin,Junhui Ma,Lei Wang,Yan Tang,Nan Ma,Yuxia Zhao,Wei Zhang
Microbial colonization and infection of biomedical devices remain a persistent clinical challenge. Interfacial coatings with antifouling and antimicrobial functions simultaneously under physiological conditions are highly desired to mitigate the infection risks. Herein, we constructed a durable serine-derived zwitterionic antifouling and antimicrobial interfacial structure via amino-carboxylate coordination with Cu(II) ions in alkaline aqueous solutions. Comprehensive surface characterizations verified the successful incorporation of Cu(II) into serine-based zwitterionic polymeric brushes without changing the valence state. The engineered interfaces maintained favorable hydrophilicity with water contact angles ranging between 16° and 24°, which contributed to their remarkable antifouling activities against bacteria, red blood cells, and platelet adhesion. Crucially, the surface exhibited broad-spectrum antimicrobial efficacy against both Gram-positive and Gram-negative pathogens under physiological pH conditions without extra stimulation, achieving bactericidal rates exceeding 99%. Moreover, owing to the durable control release of Cu(II), the antibacterial rate against E. coli was still over 90% even after 28 days of exposure in PBS. Furthermore, the surface exhibited acceptable cytotoxicity while maintaining superior hemocompatibility, with the hemolysis rate below 1%. Overall, this physiological pH responsive integrated antifouling and antimicrobial interface presented a promising and convenient platform for next-generation biomedical devices requiring antifouling properties and long-term antimicrobial protection simultaneously.
研究领域:
生物材料
·
表面工程
·
抗菌涂层
·
生物医学工程
·
配位化学
·
感染控制
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产品关联
DOI
PubMed
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2018
·Vol. 27
·期 5
·1549-1557
·
IF 1.607
Liu Ting,Chen Xiaopeng,Xing Jinfeng,Ge Zhiqiang
The changes in activities and conformations of pepsin and trypsin induced by acetazolamide (ACZ) were studied with spectroscopic methods under their respective physiological pH conditions in vitro. The results showed that both the enzymes activities were obviously inhibited as increasing in acetazolamide concentration, while the inhibitory ratio of trypsin was higher than pepsin. The intrinsic fluorescence of the two enzymes was quenched by acetazolamide through static process. Acetazolamide bound trypsin in a more firmly way than pepsin. Thermodynamic analysis revealed that driving force of acetazolamide-pepsin was different from acetazolamide-trypsin. The electrostatic interaction had a significant effect on the binding process of acetazolamide-trypsin, whereas interaction of the acetazolamide-pepsin was driven by hydrogen bonding and van der Waals force. Circular dichroism spectroscopy and synchronous fluorescence showed acetazolamide induced conformational and microenvironmental changes of both the enzymes. These investigations suggested that acetazolamide at different physiological pH may have different effects on the conformations and functions of proteins through variant mechanisms. This study is helpful for understanding the changes in the function and structure of digestive enzymes influenced by acetazolamide in gastrointestinal tract in vivo.
研究领域:
生物化学
·
药理学
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酶学
·
光谱学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）