JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Liumin Huang,Jun Li,Yanlan Bi,Yanhui Xu,Yanmei Wang,Jian Wang,Dan Peng
A simple and fast normal-phase high performance liquid chromatography method for simultaneous detection of α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, sesamin, sesamolin, sesamol, and asarinin in sesame oil (SO) was investigated. Conditions were a silica gel column as the separation unit, n -hexane and tetrahydrofuran (93:7, v/v) as the mobile phase, isocratic elution, a flow rate of 0.8 mL/min, column temperature of 30 °C, and florescence detector with excitation and emission wavelengths of 295 and 330 nm. SO samples were directly dissolved in n -hexane for HPLC analysis. Results showed that the method performed wide linear ranges and good linearity (0.27–100.0 μg/mL, R 2 > 0.9996), low limits of detection (0.09–0.16 μg/mL) and quantification (0.27–0.41 μg/mL), good precision and accuracy (biases of <2.83 % and relative standard deviations of <3.77 %), good separation performance [resolution factor (1.66–6.20), separation factor (1.11–3.94), retention factor (1.08–1.42), and height of theoretical plates (0.01–0.04 mm)], and satisfactory recoveries (87.3–109.8 % for SO and 96.5–106.9 % for lard). The method was successfully applied for the simultaneous determination of the eight analytes in eleven SO samples.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2025
·
IF 1.8
Yufeng Zhao,Dan Huang,Xiangyu Wang,Baijun Chu,Yuxiu Wen,Qi Li,Xiuzhu Yu
The variations in tocopherols and phytosterols during the oxidation of walnut oils (Wen-185, Xiangling, and Dama oils) were analyzed, and their antioxidant effects were compared. Under both ambient temperature and accelerated oxidation conditions, the degradation order of tocopherols in walnut oils was α-tocopherol > γ-tocopherol > δ-tocopherol. Phytosterols exhibited less degradation than tocopherols under both oxidation conditions, and tocopherols were more strongly associated with oil oxidation than phytosterols. When δ-tocopherol degradation reached 15%–20%, the peroxide value approached 20 meq/kg, highlighting its potential as an indicator of oil oxidation. Further studies on the antioxidant effectiveness of tocopherols revealed that the optimal effect was achieved as 400 mg/kg α-tocopherol was added at Day 16 under ambient temperature. These findings suggested that δ-tocopherol can serve as an indicator of walnut oil oxidation, and appropriately timed and dosed α-tocopherol supplementation can significantly delay oxidation in walnut oils. Practical Applications : This study compared the changes and degradation of tocopherols and phytosterols in different walnut oils. The results suggested that tocopherol degraded faster than phytosterol, with a closer correlation to oil oxidation, and α-tocopherol underwent degradation preferentially. Furthermore, the antioxidant effectiveness of tocopherols was analyzed. The results showed that proper quantity and timing of α-tocopherol addition can enhance antioxidant effect, and δ-tocopherol can assess the oxidation of walnut oils as an concomitant indicator. This study has expanded the application of endogenous concomitants in the antioxidant properties of walnut oils. It has laid a theoretical foundation for the antioxidant properties of walnut oils.
研究领域:
食品化学
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油脂科学
·
抗氧化研究
·
食用油
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氧化稳定性
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Wan-zhen Li,Zi-liang Song,Jun-le Li,Jia-hui Yu,Du-jian Deng,Xiao-qing Cai,Martin J.T. Reaney,Zi-zhe Cai,Yong Wang
Linusorbs (LOs), significantly influence oil quality and sensory properties of flaxseed oil. Trp-containing LOs exhibit distinct oxidative behavior when γ-tocopherol (γ-T) is present. Polar fractions of crude flaxseed oil were stripped via silica absorption, and reintroduced (LO and γ-T) separately into the oil matrix to investigate their interaction during storage. Compared with crude oil, LOs account for 18.49% reduction of p -anisidine value, while LOs with γ-T contributed to most of the endogenous antioxidant effect in crude oil. γ-T was found to suppress oxidation of Trp-containing LO at early stage (Met form), while facilitate oxidation while at their mid-stage (MetO form, Methionine sulfoxide). In vitro oxidation shows that CLD more likely cleaved into peptide fragments, while few products retain intact ring structures. LC-MS/MS analysis and silicon simulation revealed proximity between MetO and Trp residues, facilitating inter- or intra-molecular reactions and ring structure rupture. Remarkably, the presence of γ-T facilitate these phenomena.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Xing-An Luo,Pei Zhao,Hai Zhang,Shu-Yi Feng,Ke-Xian Chen,Zhong-Xiu Chen
Dietary macromolecules are often used for food formulation. Most of the published literature usually focuses on a certain polysaccharide reducing lipid digestion, but the non-specific physical effects such as the excluded volume effect of the surrounding macromolecules, which will possibly enhance the hydrolysis of lipids, is rarely discussed. As the influence of polysaccharides on the gastrointestinal fate of α-tocopherol acetate (VE-A) encapsulated in emulsion have not been conducted so far, in this research, we study the effect of crowded medium created by polysaccharides (Ficoll400) on the hydrolysis of VE-A and its bioaccessibility. The results show that the presence of Ficoll400 increased the bioaccessibility and conversion of VE-A 1.6 times that of a dilute buffer. An enzyme-catalyzed kinetic reaction rate of VE-A hydrolysis is increased up to 3.5 times. Both the improved thermodynamic conversion yields and kinetic rates of the hydrolysis of the VE-A emulsion was further observed in a synthetic macromolecule PEG2000 medium. This research improves our understanding of the crowding effect of macromolecules on VE-A/lipid emulsion digestion.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Lijiao Kan,Shaoping Nie,Jielun Hu,Sunan Wang,Zhouya Bai,Junqiao Wang,Yaomin Zhou,Jun Jiang,Qin Zeng,Ke Song
Twenty-nine legumes were assessed for their nutritional and phytochemical compositions. Soybean and black soybean had the highest protein contents (34.05–42.65 g/100 g DW, dry weight of legumes), particularly being a rich source of lysine (1.78–2.23 g/100 g DW. Soybean and black soybean had the highest fat contents (14.13–22.19 g/100 g DW). Broad beans had the highest unsaturated fatty acids (83.57–89.01 g/100 g fatty acid), particularly rich in α-linolenic and linoleic acid. The highest and the lowest dietary fiber were found in red kidney beans (35.36 g/100 g DW) and mung beans (22.77 g/100 g DW), respectively. Except for soybean and white kidney bean, 6 major anthocyanins in the legumes samples were identified. The soybean contained the highest total tocopherols content (90.40–120.96 μg/g dry weight of beans), followed by black soybean (66.13–100.76 μg/g DW). The highest carotenoids were found in lentils (4.53–21.34 μg/g DW) and red kidney beans (8.29–20.95 μg/g DW).
研究领域:
食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Hailu Liao,Chenlinrui Yue,Zhenni Zhai,Xiaoni Lian,Hongkai Xie,Yong Fang,Fengjiao Fan
In this study, three-layer films based on gelatin/gelatin-dextran/gelatin with different concentrations of functional fillers (cinnamaldehyde and α-tocopherol) were fabricated by using the layer-by-layer casting method to preserve refrigerated scallop adductor muscle. The films demonstrated excellent bonding, with functional fillers creating a porous cross-section, a slightly rough surface, and increased hydrophobicity while maintaining thermal stability. The crystallinity, water contact angle, thickness, opacity, antioxidant activity, and antibacterial activity of the films were increased, while the water vapor permeability and mechanical properties were decreased with increasing concentrations of the functional fillers. The film with 1.2% active fillers showed over 82% free radical scavenging activity and significant inhibition zones (24.7 ± 1.2 to 43.9 ± 1.8 mm) against five foodborne pathogens and spoilage bacteria. Both cinnamaldehyde and α-tocopherol demonstrated controlled release properties in four food simulants, with the maximum release and minimum release times observed in 95% ethanol (9.0 h) and distilled water (2.0 h), respectively. Overall, the introduction of 1.2% of active fillers into the films significantly enhanced the chemical and microbiological stability of refrigerated scallops and prolonged their shelf life by 6 days. Furthermore, the developed films showed excellent controlled release, antibacterial, and antioxidant properties, making them potential candidates for preserving seafood.
CAS / MDL:
研究领域:
食品科学
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材料科学
·
海洋生物学
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产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Nana Zhang,Anyan Wen,Xin Yang,Xinxin Zhang,Likang Qin,Haiying Zeng
Homogentisic acid (HGA), a product of the L-tyrosine metabolic pathway, serves as a substrate for tocopherol synthesis. Previous studies revealed that the fermentation of coix seeds by M . purpureus could simultaneously upregulate tocopherol and HGA levels. Therefore, this study focused on HGA to investigate its specific effect on tocopherol production during the fermentation process. The results showed that 0.02% Exo-HGA significantly increased tocopherol yield by 53.11%, reaching 184.808 μg/g. Based on the seed culture medium, the optimal formulation of key factors for promoting End-HGA synthesis in M. purpureus was determined. It was obtained through single-factor experiments and uniform design: D-fructose (36.816 g/L), L-tyrosine (0.656%), yeast extract powder (7 g/L), ZnSO 4 (0.654 g/L). Under these conditions, M. purpureus exhibited good growth, with an End-HGA yield reaching 844.461 μg/mL. In addition, fermentation of coix seeds with End-HGA led to an increase in the total tocopherol yield by 57.36%, reaching 181.303 μg/g. This result was comparable to that achieved with Exo-HGA addition. Therefore, enhancing End-HGA synthesis is an effective strategy to achieve a high tocopherol yield through M. purpureus fermentation. In conclusion, this study demonstrated that the regulation of microorganisms can enable high-level synthesis of target products and improve the efficiency of deep conversion and utilization of plant substrates.
研究领域:
生物化学
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微生物学
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食品科学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Yuling Liu,Sai Fu,Longfei Lin,Yuhong Cao,Xi Xie,Hua Yu,Meiwan Chen,Hui Li
Pluronic F127 (F127), an amphiphilic triblock copolymer, has been shown to have significant potential for drug delivery, as it is able to incorporate hydrophobic drugs and self-assemble into nanosize micelles. However, it suffers from dissociation upon dilution owing to the relatively high critical micelle concentration and lack of stimuli-responsive behavior. Here, we synthesized the α-tocopherol (TOC) modified F127 polymer (F127-SS-TOC) via a redox-sensitive disulfide bond between F127 and TOC, which formed stable micelles at relatively low critical micelle concentration and was sensitive to the intracellular redox environment. The particle size and zeta potential of the F127-SS-TOC micelles were 51.87±6.39 nm and -8.43±2.27 mV, respectively, and little changes in both particle size and zeta potential were observed within 7 days at room temperature. With 10 mM dithiothreitol stimulation, the F127-SS-TOC micelles rapidly dissociated followed by a significant change in size, which demonstrated a high reduction sensitivity of the micelles. In addition, the micelles showed a high hemocompatibility even at a high micelle concentration (1,000 μg/mL). Low cytotoxicity of the F127-SS-TOC micelles at concentrations ranging from 12.5 μg/mL to 200 μg/mL was also found on both Bel 7402 and L02 cells. Overall, our results demonstrated F127-SS-TOC micelles as a stable and safe aqueous formulation with a considerable potential for drug delivery.
研究领域:
药剂学
·
生物材料学
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产品关联
DOI
PubMed
高影响因子
ACTA PHYSIOLOGIAE PLANTARUM
·
2023
·Vol. 46
·期 2
·1-17
·
IF 2.6
Kumari Monika,Singh Hidam Bishworjit,Khan Mohammad Imtiyaj
Betalains are health-promoting plant pigments accumulated in non-anthocyanic plants. Owing to its limited distribution in nature, metabolic trade-offs of inhibiting them are poorly understood. The aim of this study was to investigate the effects of inhibition of betalains in Amaranthus tricolor seedlings to gain insights into the relationship of betalain biosynthesis with other biosynthetic pathways in betalain-accumulating plants. Phenylalanine (Phe; 12.5, 25, and 50 mmol L −1 ) and 3-methyl-2-benzothiazolinone hydrazone (MBTH) (25 mmol L −1 ) were treated to inhibit betalain biosynthesis in ten-day-old A. tricolor (red) seedlings. After two and six days of treatment, target (betalain-related metabolites) and non-target metabolites were analyzed. In two days, Phe content increased by 2.6, 8.5, and 17.4-fold in Phe (12.5, 25, and 50 mmol L −1 )-treated seedlings, respectively, compared to control, indicating the uptake of Phe by the seedlings. Phe treatment led to a 10.1–18% decrease in betacyanins, while MBTH caused an 18.3% decrease in two days. In both treatments, cyclo -DOPA formation, which is essential for betacyanin biosynthesis, seems to be inhibited, albeit through different mechanisms. Betalain biosynthetic precursors and intermediates, viz., tyrosine, L-DOPA, and dopamine decreased differentially. Ascorbic acid, α -tocopherol, and retinol contents increased in both treatments concomitant with the reduction in betalains, total phenols, and antioxidant enzymes. Therefore, Phe treatment is beneficial in enhancing antioxidant metabolites in betalain-accumulating plants. However, the mechanism of increasing ascorbic acid on inhibiting betalains needs further investigation in other betalain-producing plants also to understand if ascorbic acid is involved in regulating betalain biosynthesis.
研究领域:
植物学
·
代谢生物学
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产品关联
DOI
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 3
·564
·
IF 7
Lingling Ma,Tiecheng Gao,Hao Cheng,Ning Li,Weining Huang,Li Liang
Protein particles have been reported as the potential carriers for the co-encapsulation of bioactive components. In this study, lysozyme, a basic protein, was used to simultaneously encapsulate folic acid andα-tocopherol at pH 4.0. The encapsulation efficiency and loading capacity of folic acid orα-tocopherol increased with its respective concentration. Folic acid had no influence on the encapsulation ofα-tocopherol. However, the encapsulation of folic acid was improved byα-tocopherol below 40 μg/mL but reduced byα-tocopherol at higher concentrations. The encapsulation by lysozyme shielded folic acid,α-tocopherol, or both partially from the attack of 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical cation. No masking effect of lysozyme encapsulation onα-tocopherol was found in DPPH antioxidant activity assay. Furthermore, the DNA coating was used to improve the dispersion of lysozyme with folic acid andα-tocopherol. The lysozyme/DNA particles with folic acid andα-tocopherol showed a homogenous size distribution of 180–220 nm with ζ-potential values between −33 and −36 mV. The release and bioaccessibility of folic acid in lysozyme/DNA withα-tocopherol were similar to that of folic acid alone, while the release ofα-tocopherol was delayed and its bioaccessibility was improved by encapsulation in lysozyme/DNA with folic acid. The data gathered here would provide guidance for the use of lysozyme-based co-encapsulating carriers in the development of functional foods.Keywords:lysozyme;folic acid;α-tocopherol;DNA coating;encapsulation;bioaccessibility
研究领域:
食品科学
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营养学
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生物技术
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Lei He,Yongze Liu,Haomiao Zhu,Feng Chen,Jiong Shi,Panpan Song,Fuqi Feng,Juqing Cui,Xiaofeng Ma,Jun Zhang
Nasal diseases are pervasive infections that can impair the quality of life of sufferers. In this study, a flexible modified triplochitin scleroxylon (MTS)-based Janus hydrogel (MTS@P/DLT) was obtained by disrupting the homogeneity of the system using DL-α-tocopherol (DLT) with flexible wood as the framework and poly(vinyl alcohol) (PVA) as the outer matrix. At a PVA concentration of 10 wt %, DLT formed a homogeneous layer, as revealed by electron microscopy. Mechanical property analysis showed that chemical and hydrogel modifications significantly increased the flexibility and toughness of the hydrogel. Microscopic and macroscopic assessment of the characteristics of the two sides of the MTS@P/DLT hydrogel revealed that one side of the DLT film had antiadhesion effects. Molecular dynamics simulation was used to determine the movement law of DLT in a PVA solution. The stronger intermolecular force between DLT and PVA than that between DLT and water, as well as between DLT molecules, enabled the formation of a homogeneous DLT film by controlling the concentration of PVA. In vitro and in vivo models demonstrated the potential of MTS@P/DLT to resist infection and facilitate recovery. Thus, MTS@P/DLT is promising for medical use.
研究领域:
材料科学
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医学
·
生物工程
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产品关联
DOI
高影响因子
Journal of Biobased Materials and Bioenergy
·
2020
·Vol. 14
·期 5
·657-663
·
IF 1.126
Liu Jie,Wu Lichao,Tan Yimin
Se-enriched camellia oil has received increasing attention because it can improve the Se intake of diet for Se deficiency areas and has a wide spectrum of biological effects. Six main cultivars of camellia oil in Guangxi, including Camellia oleifera cenxiruanzhi 2 (CR2), CR3,
C. oleifera xianglin 4 (XL4), XL40, C. oleifera changlin 1 (CL1), and CL210, were selected for fertilization experiment to produce Se-enriched camellia oil and investigated the effect of Se enrichment on quality. The Se content in camellia oil of six varieties was significantly increased
by Se fertilizer application. CR2 had the maximum Se content and raise ratio compared to CK, which was 0.87 mg kg−1 and fourfold, respectively. The Se contents in XL1, XL210, CR3, CL4, and CL40 improved by 2.14-, 2.77-, 3.58-, 2.56-, and 3.79-fold, respectively. Se fertilizer
reduced the acid and peroxide values and increased the squalene and tocopherol contents. The acid value decreased by 38.29%–61.30%, the peroxide value decreased by 20.66%–61.79%, the tocopherol content increased by 7.76%–32.78%, and the squalene content increased by 13.53%–77.39%.
The quality indices were significantly different among camellia oil varieties. Principal component analysis showed that CR2 obtained the highest score by comprehensive evaluation. Se fertilization, particularly for CR2 species, can significantly increase the contents of Se as well as functional
components, such as squalene and tocopherol, and thus improve the quality of camellia oil
研究领域:
植物学
·
营养学
·
食品科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）