Metabolites
·
2022
·Vol. 12
·期 5
·444
·
IF 5.581
Muhammad Khalid Hameed,Wajid Umar,Ali Razzaq,Tariq Aziz,Muhammad Aamer Maqsood,Shiwei Wei,Qingliang Niu,Danfeng Huang,Liying Chang
Nitrogen (N) is an essential element for plant growth and development. The application of a balanced and optimal amount of N is required for sustainable plant yield. For this, different N sources and forms are used, that including ammonium (NH4+) and nitrate (NO3−). These are the main sources for N uptake by plants where NH4+/NO3−ratios have a significant effect on the biomass, quality and metabolites composition of lettuce grown in soil, substrate and hydroponic cultivation systems. A limited supply of N resulted in the reduction in the biomass, quality and overall yield of lettuce. Additionally, different types of metabolites were produced with varying concentrations of N sources and can be used as metabolic markers to improve the N use efficiency. To investigate the differential metabolic activity, we planted lettuce with different NH4+/NO3−ratios (100:0, 75:25, 50:50, 25:75 and 0:100%) and a control (no additional N applied) in soil, substrate and hydroponic cultivation systems. The results revealed that the 25% NH4+/75% NO3−ratio increased the relative chlorophyll contents as well as the biomass of lettuce in all cultivation systems. However, lettuce grown in the hydroponic cultivation system showed the best results. The concentration of essential amino acids including alanine, valine, leucine, lysine, proline and serine increased in soil and hydroponically grown lettuce treated with the 25% NH4+/75% NO3−ratio. The taste and quality-related compounds in lettuce showed maximum relative abundance with the 25% NH4+/75% NO3−ratio, except ascorbate (grown in soil) and lactupicrin (grown in substrate), which showed maximum relative abundance in the 50% NH4+/50% NO3−ratio and control treatments, respectively. Moreover, 1-O-caffeoylglucose, 1,3-dicaffeoylquinic acid, aesculetin and quercetin-3-galactoside were increased by the application of the 100% NH4+/0% NO3−ratio in soil-grown lettuce. The 25% NH4+/75% NO3−ratio was more suitable in the hydroponic cultivation system to obtain increased lettuce biomass. The metabolic profiling of lettuce showed different behaviors when applying different NH4+/NO3−ratios. Therefore, the majority of the parameters were largely influenced by the 25% NH4+/75% NO3−ratio, which resulted in the hyper-accumulation of health-promoting compounds in lettuce. In conclusion, the optimal N applications improve the quality of lettuce grown in soil, substrate and hydroponic cultivation systems which ultimately boost the nutritional value of lettuce.Keywords:ammonium;lettuce;metabolites;nitrogen;nitrate
研究领域:
农业
·
植物科学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 56
·85454-85464
·
IF 5.19
Wang Wenfeng,Yuan Shu,Kwon Jung-Hwan
The prevalence of per- and polyfluoroalkyl substances (PFASs) in agricultural soils has raised concerns regarding the health risks associated with the consumption of PFAS-contaminated agricultural products. The present study investigated the uptake and translocation of nine PFASs in lettuce using a hydroponic setting. During the uptake experiments, long-chain PFASs (≥ C8) exhibited greater accumulations in lettuce roots, while short-chain PFASs (≤ C7) manifested preferential transport to the shoots. The average root concentration factors of PFASs were positively correlated with their log K ow values. A significantly negative relationship was found between the average translocation factors of PFASs and their molecular volume. Sorption of long-chain PFASs by lettuce roots was enhanced after heating the roots to increase the cell membrane permeability. The accumulation of perfluorododecanoic acid increased significantly in shoots of lettuce plants without roots as compared to whole lettuce plants. Results of the present study indicate that sorption to root surface tissues and efficiency in passing through the root Casparian strip are two important factors that affect the uptake and distribution of PFASs within plants.
研究领域:
环境科学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 12
·2976
·
IF 4.35
Jianying Zhao,Jing Qian,Hong Zhuang,Ji Luo,Mingming Huang,Wenjing Yan,Jianhao Zhang
This study aimed to investigate effects of plasma-activated solution (PAS) on the cell biology ofStaphylococcus aureusand qualities of fresh lettuce leaves. PAS was prepared by dielectric barrier discharge plasma and incubated withS. aureusfor 10–30 min or with lettuces for 10 min. Effects on cell biology were evaluated with microscopic images, cell integrity, and chemical modification of cellular components. Effects on lettuce quality were estimated with the viable microbial counts, color, contents of vitamin C and chlorophyll, and surface integrity. PAS reducedS. aureuspopulation by 4.95-log and resulted in increased cell membrane leakage. It also resulted in increased contents of reactive oxygen species in cells, C=O bonds in peptidoglycan, and 8-hydroxydeoxyguanosine content in cellular DNA, and reduced ratios of unsaturated/saturated fatty acids in the cell membrane. PAS treatment reduced bacterial load on fresh lettuce and had no negative effects on the quality. Data suggest that PAS can be used for the disinfection of ready-to-eat fresh vegetables.Keywords:reactive oxygen species;peptidoglycan;cell membrane;DNA;lettuces
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2023
·
IF 4.3
Muhammad Khalid Hameed,Wajid Umar,Ali Razzaq,Shiwei Wei,Qingliang Niu,Danfeng Huang,Liying Chang
Lettuce is an important leafy vegetable and an excellent source of phytochemicals in human diets. However, the metabolic levels in different green and red type lettuce varieties are not fully explored. Therefore, it is important to explore and identify the selected health promoting secondary metabolites in green and red type lettuce varieties with having different phenotypic characteristics. In this study, we explored the selected secondary metabolites, total polyphenol, antioxidants activities, total flavonoids and anthocyanin contents in 32 lettuce varieties of green and red color. The results showed that green and red type lettuce varieties showed a significant difference in lactupicrin, chlorogenic acid, caffeoylmalic acid, syringic acid and quercetin 3-D-galactoside. Selected metabolites showed a significant variation in green and red type lettuce varieties depends upon different color and phenotypic characteristics. Total flavonoids and anthocyanin contents were found more in red type lettuce varieties while the total polyphenols and total antioxidants were found significantly higher in green type lettuce varieties. Tentative identified 78 metabolites with online databases, the published literature and standards including phenolic acid, flavonoids and glycosylated flavonoids. The metabolomic profiling was present at significantly difference in green and red type lettuce varieties which suggested the significant variation between green and red type lettuce varieties to secondary metabolism. This study provides a selection of lettuce varieties for further studies on the basis of color and phenotypic characteristics for improving the nutritional value, quality and yield of lettuce.
研究领域:
植物科学
·
营养学
·
分析化学
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
ACS Agricultural Science & Technology
·
2022
·
IF 0
Zhiwei Huang,Baoyan Guo,Yujie Zou,Jingyi He,Chaofan Hu,Jianle Zhuang,Yingliang Liu
Herein, we synthesized different kinds of citric acid based CDs from low-cost citric acid and used them for culturing Italian lettuce. Since they all used citric acid as a precursor, some similarities were observed in terms of optical properties, morphologies, and chemical compositions. In comparison to the control group, they were able to increase the fresh weight of lettuce by 25.53% (CA-CDs), 33.25% (EDA-CDs), and 39.5% (Mg,N-CDs) respectively. Moreover, the three CDs can enhance the nutrient qualities of lettuce. After evaluating the promotion effect of three kinds of CDs on the growth and nutritional qualities of lettuce, we found that the promotion effect of Mg,N-CDs was the best among the three. The results of PAM chlorophyll fluorescence imaging indicate that Mg,N-CDs can promote photosynthesis, thereby increasing weight and accumulating nutrients. This work provides guidance for designing CDs for agricultural production.
研究领域:
农业科学
·
植物生理学
✓ 已验证
产品关联
DOI
Environmental Technology & Innovation
·
2025
·
IF 7.1
Hua Huang,Xiaoyan Liu,Xianying Shan,Jingrong Yang,Lijuan Ding,Rongyu Zhang,Jiale Dai,Yanzhong Zhen,Jian Wang,Di Guo,Yu Liu,Wenfeng Jiang,Zhirui Niu
Since hydrothermal aqueous phase (HAP) contains abundant nutrients and humic-like substances, it is developed as nitrogen (N) fertilizer to improve plant growth and soil quality. However, significant NH 3 volatilization is a major drawback in dryland soil. To solve this problem, a compound fertilizer of HAP and urea was modified with various dosage (0.25 % - 5.0 %) of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). The NH 3 , N loss, and lettuce growth were evaluated in parallel soil column and pot experiments. Results showed that increasing NBPT dosage increased soil dissolved organic N (DON) and ON while decreased NH 4 -N, NH 3 , N loss, urease activity, and ureC . NBPT successfully inhibited ammonification. Increasing NBPT dosage increased NO 3 -N but decreased PAO and AOA . The utilization rate of metabolic substrate (NH 4 -N) was more important for forming NO 3 -N, as evidenced by the negative correlation of NO 3 -N and NH 3 . The DON from HAP included N-heterocyclic compounds, which are toxic for nitrifiers. The toxic DON also significantly decreased root dry weight by 22.31 % when 5.0 % NBPT was added. The optimal additive dosages were 0.5 % and 2.0 % in the column and pot experiments, respectively, which decreased NH 3 and N loss by 45.38 % and 32.08 %. Further increasing NBPT dosage no longer decreased N loss but did harm to plant root. Therefore, this study demonstrated that an appropriate addition of NBPT to HAP fertilizer can effectively reduce ammonia volatilization and N loss. The innovative technique would promote the utilization of HAP resource within the context of sustainable dryland agriculture.
研究领域:
农业科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
Environmental Science-Processes & Impacts
·
2022
·Vol. 24
·期 4
·586-597
·
IF 5.334
Dongqing Gong,Xue Bai,Yuzhu Weng,Mengen Kang,Yue Huang,Fengjie Li,Yanling Chen
Nanoplastics and metal oxide nanoparticles are serious threats that inevitably enter the environment. Their similar particle properties likely lead to interaction and thus cause more unpredictable ecotoxicity to organisms. In this study, it was found that polystyrene nanoplastics (PS NPs) aggravate the toxic effect of iron oxide nanoparticles (Fe2O3 NPs) on Lactuca sativa L. by inducing severe oxidative stress and root deformation, and the expansion of damaged cells from the xylem to the epidermis was observed using confocal laser scanning. Exposure to PS NPs + Fe2O3 NPs correspondingly elevated iron accumulation in the roots and leaves by 1.39 and 1.17 times compared to the amount observed with Fe2O3 NPs individually. Examination of the physicochemical properties, iron ion release, and molecular interactions of the NPs indicated that PS NPs interact with Fe2O3 NPs to form heteroaggregates and facilitate leaching of iron ions, which resulted in aggravating the toxic effect. These were alleviated by the addition of humic acid (HA), which dispersed the heteroaggregates and reduced the release of iron ions. The findings in the present study provide new perspectives for the ecotoxicological risk of binary nano-pollution in the natural environment.
研究领域:
环境科学
·
植物毒理学
✓ 已验证
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2025
·
IF 4.3
Xuancheng Yuan,Bochen Zhang,Fan Zhang,Zhuang Wang
Micro/nanoplastics (MNPs) can serve as vectors for engineered nanoparticles (ENPs), thereby eliciting mixture toxicological responses in target species. However, our understanding of the combined environmental impacts of lighting conditions and co-exposure to MNPs and ENPs on crop plants remains limited. Herein, we investigated the individual and joint effects of polystyrene microplastics (PMs; 1 μm) or nanoplastics (PNs; 50–100 nm) and ZnO nanoparticles (ZnO NPs) on seed germination and early seedling growth of lettuce ( Lactuca sativa ) under the dark condition or the light–dark cycle condition. The results showed that the joint toxic effects of PMs/PNs and ZnO NPs on seed germination were antagonistic, regardless of lighting conditions. Furthermore, the coexistence of PMs/PNs attenuated the inhibitory effect of ZnO NPs on the total photosynthetic pigment content in seedling shoots. Under the light–dark cycle condition, the translocation factor values of zinc from seedling roots to shoots when exposed to PMs or PNs with ZnO NPs exceeded 1, indicating that zinc in the form of ZnO NPs easily migrated from seedling roots to shoots. When ZnO NPs were combined with either PMs or PNs under varying light conditions, a consistent alleviation of oxidative stress was observed in the seedlings compared to individual particle treatments. This finding may represent a key cellular mechanism underlying the antagonistic effects between PMs/PNs and ZnO NPs. Additionally, the expression of Cu/Zn-superoxide dismutase gene in seedlings was differentially modulated in response to both individual and combined exposures to PMs/PNs and ZnO NPs. Collectively, this study provides comprehensive insights into the cellular and molecular responses of agricultural crops under complex environmental conditions.
研究领域:
环境科学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2025
·
IF 9.8
Ying Wang,Jiayu Liu,Xinyan Fan,Yunjie Ju,Zhenke Wei,Xiangyu Tang,Yonggui Wang,Zefang Xiao,Yanjun Xie
Improving photosynthesis and light capture using bio-based light-conversion films increases crop yield and paves a sustainable way to meet the growing global food demand. In this study, red-emissive carbon dots (RCDs) and blue-emissive CDs (BCDs) were trapped in biopolymers to develop a novel dual-excitation dual-emission light-conversion film (BRLCFs) with sandwich structure for efficient solar spectral conversion and promote photosynthetic efficiency of greenhouse plants. The middle layer of the film comprises polyvinyl alcohol (PVA), RCDs, and ascorbic acid (AA), which convert ultraviolet and green light into red light. The strong hydrogen bonding between the RCDs and PVA, including the antioxidant properties of AA, ensured excellent stability of the red light emission of the film. The edge layers, which composed of cellulose acetate and BCDs, converted ultraviolet light into blue light. This composition allowed the film to maintain exceptional photostability and morphological stability, even in high-humidity environments. In agricultural cultivation, BRLCFs significantly enhanced lettuce growth parameters, with a notable 21.89 % increase in fresh weight and 19.54 % increase in dry weight, highlighting the potential of BRLCFs to boost crop yield in controlled environments.
研究领域:
农业科学
·
材料科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
ACS Agricultural Science & Technology
·
2024
·
IF 2.3
Lei Xiang,Rui-Xin Li,Qing-Jun Zheng,Zan-Tang Huang,Peng-Fei Yu,Zhuo-Xing Shi,Yan-Wen Li,Hai-Ming Zhao,Quan-Ying Cai,Xue-Wen Hou,Ce-Hui Mo,Qing X. Li
Plant protoplasts are very useful in plant biotechnology, molecular biology, and cell biology. However, an efficient method for protoplast production remains a challenge for many economically important dicotyledonous vegetables, including lettuce (Lactuca sativa L.). Herein, a protocol was optimized for efficient protoplast production from various tissues (leaf and shoot apex) of different lettuce subtypes (romaine, loose-leaf, and semiheading lettuces) by optimizing the major factors affecting protoplast yielding. The optimized protocol yields protoplasts up to 2.0 × 107/g (fresh weight, FW) with viability more than 85%, which is 3–10 times higher than those previously reported. This optimized protocol was also found to be applicable to other dicotyledonous plants (bok choy (Brassica parachinensis), celery cabbage (Brassica pekinensis), and Arabidopsis thaliana) for efficient protoplast production from leaves and shoot apexes. Moreover, an optimized poly(ethylene glycol)-mediated transient expression system (TES), using lettuce shoot apex protoplasts generated via the aforementioned protocol, exhibited high transfection efficiency exceeding 80%. This was further evidenced by the elevated expression levels and subcellular localization of four representative plasma membrane transporters: AAP2, ABCG22, ALMT10, and OATP. In conclusion, the optimized protoplast production protocol along with TES developed in this study will be useful tools for the functional analyses of genes in lettuce and other important dicotyledonous vegetables.
研究领域:
植物学
·
分子生物学
·
植物生物技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Qing Cao,Alan D. Steinman,Xiang Wan,Liqiang Xie
Microcystins and copper commonly co-exist in the natural environment, but their combined toxicity remains unclear, especially in terrestrial plants. The present study investigated the toxicity effects of microcystin-LR (0, 5, 50, 500, 1000 μg L −1 ) and copper (0, 50, 500, 1000, 2000 μg L −1 ), both individually and in mixture, on the germination, growth and oxidative response of lettuce. The bioaccumulation of microcystin-LR and copper was also evaluated. Results showed that the decrease in lettuce germination induced by copper alone was not significantly different from that induced by the mixture, and the combined toxicity assessment showed a simple additive effect. Lettuce growth was not significantly reduced by microcystin-LR alone, whereas it was significantly reduced by copper alone and the mixture when copper concentration was higher than 500 μg L −1 . High concentrations of microcystin-LR (1000 μg L −1 ) and copper (≥50 μg L −1 ),as well as their mixture (≥50 + 500 μg L −1 ), induced oxidative stress in lettuce. A synergistic effect on the growth and antioxidative system of lettuce was observed when exposed to low concentrations of the mixture (≤50 + 500 μg L −1 ), whereas an antagonistic effect was observed at high concentrations (≥1000 + 2000 μg L −1 ). Moreover, the interaction of microcystin-LR and copper can increase their accumulation in lettuce. Our results suggest that the toxicity effects of microcystin-LR and copper are exacerbated when they co-exist in the natural environment at low concentrations, which not only negatively affects plant growth but also poses a potential risk to human health via the food chain.
研究领域:
环境科学
·
植物生理学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Wen-Jing Wei,Lei Li,Yu-Pei Gao,Qiang Wang,Yao-Yu Zhou,Xin Liu,Yuan Yang
Plants can absorb and accumulate engineered nanomaterials (ENMs) through water and soil, providing a potential way for nanoparticles to be enriched in humans through the food chain. In this paper, a combination of enzymatic digestion method and SP-ICP-MS analysis was used to quantitatively characterize the enriched AuNPs in mustard and lettuce plants. The results showed that Macerozyme R-10 enzyme can extract AuNPs from plants without obvious aggregation/dissolution. Both mustard and lettuce plants can absorb and enrich the complete AuNPs to the above-ground organs, and the particle number concentrations detected are 1.24 × 10 7 particles L −1 and 4.39 × 10 7 particles L −1 , respectively. With different exposure level of AuNPs(0.5 mg L −1 ,), a particle number concentration of 2.32 × 10 7 particles L −1 was detected in the stems of lettuce plants, while the mustard failed to transport AuNPs to the above-ground organs. The transport efficiency of Au ions by plants is higher than that of AuNPs, and the plants have stronger bioavailability for ions.
研究领域:
植物学
·
环境科学
·
纳米毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）