EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2017
·Vol. 244
·期 5
·851-860
·
IF 1.664
Xu Dan,Liu Hong,Jin Cheng-yu,Cao Chun-mei,Li Wen-gang,Zeng Fan-kui,Zhao Yu-ci,Liu Gang
Hongmei is a new potato variety developed in recent year, which is suitable for raw eating as a kind of fruit. In this study, sensory evaluations, nutritional components, morphology characteristics of starch granules, phenolic compounds, sugars, glycoalkaloids, and amino acid composition of potato Hongmei were investigated. The results show that Hongmei be suitable for raw eating mainly based on three reasons: (1) possess a juicy characteristic and lower earthy smell, with a distinctive fruity flavor of fresh cucumber; (2) the average particle size of starch from Hongmei is 18.31 ± 7.71 μm, which is smaller than the commercial potato starch significant ( p < 0.01); (3) with a content of 653.64 ± 83.25 mg/100 g of fructose, which is similar to the sweet potato be suitable for raw eating. The results also show that Hongmei had high concentrations of crude protein, total ascorbic acid, but lower starch content. The total glycoalkaloids content in the flesh is only 5.52 mg/kg, which implied that it is safe for raw eating. However, due to the total glycoalkaloids in the Hongmei peel is 358.88 mg/kg, which is higher than the safe standard of 200 mg/kg, it is recommended to peel when eating raw.
研究领域:
食品科学
·
植物学
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Xiaoyu Liu,Xianli Wang,Shuang Hong,Huatian Zhou,Xiaolong Cao,Kepiao Li,Qicai Zhang,Chunxia Yao,Wanchao Chen,Wen Li,Weiguo Song,Qinxiong Rao
Per-and polyfluoroalkyl substances (PFASs) have garnered significant attention owing to their prevalence and adverse effects on humans. The direct dietary intake of perfluoroalkyl acids (PFAAs) and PFAAs precursors (pre-PFAAs) biotransformation are considered major contributors to human exposure to PFASs. However, little information is available on analytical methods for the simultaneous detection of PFAAs and pre-PFAAs. In the present study, a single-step sample-treatment-based supramolecular solvents-dispersed liquid-liquid microextraction (SUPRASs-DLLME) technique was established for the analysis of 16 PFAAs and 7 pre-PFAAs in aquatic food. SUPRASs were synthesized using 1-heptanol (3 mL) and tetrahydrofuran (4 mL), which were self-assembled in water. The parameters for microextraction, such as extraction method and enrichment capacity, were optimized. Under the optimum conditions, the limit of detection (LOD) and limit of quantification (LOQ) were 0.03–0.15 ng·g −1 and 0.1–0.5 ng·g −1 , respectively. Good linearities (R 2 > 0.996) were obtained for all the target compounds, and the recoveries ranged 81.1–120 % with relative standard deviations (RSDs) lower than 20 %. This method was applied to the analysis of 16 PFAAs and 7 pre-PFAAs in aquatic food samples (crabs, prawns, and fish). This study provides a new idea for analyzing other pollutants in biological samples.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Zhixin Fei,Hang Zhang,Qing Song,Mingyue Sun,Chengxi Li,Min Liu
Ubiquitous perchlorate contamination has become an important food safety issue related to human health, but contamination characteristics and health risks of perchlorate in wild edible mushrooms remain unclear. In this study, a method based on dispersive solid phase extraction (d-SPE) clean-up procedure combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established for the rapid analysis of perchlorate in wild edible mushrooms. Sample pretreatment was achieved by acetonitrile extraction followed by clean-up with florisil and graphitized carbon black (GCB). The proposed method presented satisfactory linearity, accuracy, precision, negligible matrix effects, and low limit of detection (LOD) (0.15 µg/kg) and limit of quantification (LOQ) (0.5 µg/kg). The recoveries at four levels (0.5, 3, 30 and 300 µg/kg) were 93.1–108.7% with relative standard deviation ranging from 2.9% to 18.2% for mushroom samples. The validated methodology was successfully applied to detect 109 wild edible mushroom samples belonging to 8 species collected from Yunnan, China, and 95.41% of the samples were found to have detectable perchlorate contamination. Concentrations of perchlorate ranged from below LOD to 29.81 μg/kg, with a mean value of 4.11 μg/kg. The risk assessment results for perchlorate in wild edible mushrooms showed no health risks to consumers.
研究领域:
食品科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 12
·2908-2919
·
IF 2.413
Xie Zenghui,Zhang Lanyun,Chen Yanli,Hu Xujia
In this study, novel magnetic molecularly imprinted polymers (MMIPs) with specific adsorption for glutathione were prepared. Based on MMIPs as adsorbents for magnetic solid-phase extraction, we combined magnetic solid-phase extraction with high-performance liquid chromatography (MPSE-HPLC) for the separation, enrichment, and detection of glutathione in nine kinds of wild edible bolete extracts. MMIPs were characterized by Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM). In addition, the properties of MMIPs were studied by adsorption isotherm, adsorption kinetic, and selective adsorption experiments. The results showed that MMIPs had high selectivity for glutathione ( K d = 245.33 mL/g), and adsorption time and saturated adsorption capacity were 30 min and 17.88 μmol/g, respectively. Under the optimized conditions, there was a good linear relationship in the concentration range of 100~3000 μg/L, the correlation coefficient R 2 = 0.9997, and the limit of detection (LOD) and the limit of quantitation (LOQ) that were 24.828 μg/L and 82.760 μg/L, respectively. The spiked recovery and RSD were 93.28~103.03% and 2.77~5.11, respectively. We successfully combined MSPE-HPLC for the detection of glutathione in nine kinds of wild edible bolete extracts. It was found that the content of glutathione in Leccinum extremiorientale (L. Vass.) Sing. was the highest (19.800 mg/g) and that in Boletus aereus was the lowest (6.791 mg/g).
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2021
·
IF 3.512
Zili Song,Ruilin Zhao,Hongjiao Zhang,Penglin Wei,Landa Qi,Guocan Chen,Wen-Bing Yin,Wei Li
Edible mushrooms are important nutraceutical sources of foods and drugs, which can produce various nutritional ingredients including all essential amino acids. The method of rapid screening for the strains producing specific functional components is very indispensable. Homocitrate synthase is one of the key enzymes in the α-aminoadipate pathway for lysine biosynthesis and has preferable sequence conservation in Agaricales. Based on the blast of homocitrate synthase homologous genes of strains of Agaricales, we achieved combinations of degenerate primers as molecular markers to rapidly screen the lysine-producing edible mushrooms. The experimental results revealed that the consistency between PCR amplification and HPLC analysis attained 82 and 75% in strains of Agaricales and Polyporales, respectively. The finding showed that the molecular marker has higher universality for screening edible mushroom resources of Agaricales. This PCR-based approach shows excellent potential in evaluating and discriminating edible wild-grown mushrooms with high lysine content in Agaricales.
研究领域:
真菌学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Food Research
·
2024
·
IF 4.5
Nali Zhou,Hanyue Zhang,Shuna Xiang,Yingting Lin,Ying Shang
Multiplex ligation-dependent probe amplification (MLPA) is a high-throughput detection technology with superior specificity and sensitivity, which combines ligation-dependent probe hybridization and PCR amplification. However, the whole process requires repeated temperature change, and the detection results need high resolution agarose electrophoresis or capillary electrophoresis, which limits its application. In viewing of its advantages, an isothermal reaction process based on that combined Hybridization chain reaction (HCR) and hydrogel technology is developed, in order to achieve visual output of detection results without relying on any large instruments and equipment. The edible mushroom is taken as the detection target, and the LP probes were designed according to its reported endogenous reference gene. Through optimizing the reaction conditions, only the samples contained target component, the result showed irregular hydrogel. This visual detection system had good specificity, which detection limit was as low as 0.32 ng/μL. The development of this method provides a new strategy for species identification and adulteration detection of edible mushroom, even for more DNA target detection.
研究领域:
分子生物学
·
生物技术
·
食品科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）