International Journal of Extreme Manufacturing
·
2025
·Vol. 7
·期 3
·035001
·
IF 21.3
Fan-Chun Bin,Xin-Yi Wu,Jie Liu,Xian-Zi Dong,Teng Li,Qi Duan,Jian-Miao Zhang,Katsumasa Fujita,Mei-Ling Zheng
Chitosan (CS)-based nanocomposites have been studied in various fields, requiring a more facile and efficient technique to fabricate nanoparticles with customized structures. In this study, Ag@methacrylamide CS/poly(ethylene glycol) diacrylate (Ag@MP) micropatterns are successfully fabricated by femtosecond laser maskless optical projection lithography (Fs-MOPL) for the first time. The formation mechanism of core-shell nanomaterial is demonstrated by the local surface plasmon resonances and the nucleation and growth theory. Amino and hydroxyl groups greatly affect the number of Ag@MP nanocomposites, which is further verified by replacing MCS with methacrylated bovine serum albumin and hyaluronic acid methacryloyl, respectively. Besides, the performance of the surface-enhanced Raman scattering, cytotoxicity, cell proliferation, and antibacterial was investigated on Ag@MP micropatterns. Therefore, the proposed protocol to prepare hydrogel core-shell micropattern by the home-built Fs-MOPL technique is prospective for potential applications in the biomedical and biotechnological fields, such as biosensors, cell imaging, and antimicrobial.
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JOURNAL OF CONTROLLED RELEASE
·
2026
·
IF 12.4
Jingxia Chen,Xiuyu Liu,Jiaxin Luo,Yihan Wang,Zhen Ci,Yangfan Pei,Siyu Chen,Sicong Ren,Jingjie Zhai,Huixin Lv,Yanmin Zhou
Critical-sized bone defects pose a significant clinical challenge, with inadequate blood supply being a major factor impairing bone healing. In the initial stage of bone healing, the blood clot acts as a “natural glue,” serving not only as a scaffold for stem cell growth but also providing a reservoir of cytokines and growth factors that regulate and reconstruct the osteogenic microenvironment. In this study, we hierarchically integrated platelet concentrates with distinct functions into an injectable thermosensitive hydrogel, constructing a “biomimetic blood clot” system. First, leukocyte-platelet-rich fibrin (L-PRF) lyophilized powder, which promotes the osteogenic differentiation of periosteum-derived mesenchymal stem cells (PMSCs), was incorporated into methacrylated gelatin hydrogel microspheres (GM) to form GM L-PRF . Subsequently, advanced platelet-rich fibrin (A-PRF) lyophilized powder, which enhances the angiogenic differentiation of lamina propria-derived mesenchymal stem cells (LMSCs), was blended into a hydrogel precursor composed of chitosan (CS), polygalacturonic acid (PgA), β-glycerophosphate (β-GP), and GM L-PRF . This yielded a thermosensitive hydrogel, designated CSPgA A-PRF GM L-PRF (CP A G L ), which exhibits favorable mechanical properties at 37 °C and enables the programmed release of growth factors from both A-PRF and L-PRF. The CP A G L hydrogel could induce the differential differentiation of distinct types of MSCs. It facilitates vascularized bone regeneration by activating the JAK2-STAT3/HIF-1α and the TGF-β/Wnt signaling pathway, thereby effectively initiating the coupled an gio -osteogenic process. In summary, the biomimetic blood clot system CSPgA A-PRF GM L-PRF represents a potential injectable biomaterial strategy for bone defect repair.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yuanbiao Ma,Xu Liu,Zhewen Deng,Jiaqi Li,Ruoxi Liu,Shanshan Li,Can Zhang,Yutong Chen,Senqiang Zhu,Qi Zhang,Rui Liu,Bo Ma
CMCSMA/F127/TPGS@α-MG hydrogel promotes infected diabetic wound healing. • F127/TPGS micelles improve the aqueous solubility and sustained release of α-MG. • The composite hydrogel disrupts biofilms and achieves 99.6% wound closure. • α-MG modulates PPAR-γ, attenuating inflammation and promoting angiogenesis. Diabetic wounds pose a major clinical challenge owing to their complex microenvironment and persistent inflammation. To address this issue, we engineered a multifunctional, in situ photocrosslinkable composite hydrogel (CMCSMA/F127/TPGS@α-MG) to promote wound healing. To overcome the hydrophobicity of the antimicrobial agent α-mangostin (α-MG), it was first encapsulated in F127/TPGS nanomicelles. These micelles were then integrated into a methacrylated carboxymethyl chitosan (CMCSMA) matrix, enabling rapid conformal gelation upon 405 nm light irradiation. The resulting hydrogel exhibited a biphasic sustained-release profile, excellent biocompatibility, and potent contact-killing and anti-biofilm activities against Staphylococcus aureus ( S. aureus ). In an S. aureus -infected diabetic wound model, the hydrogel significantly accelerated wound closure, achieving 99.6% closure by day 14, and facilitated high-quality tissue regeneration characterized by complete re-epithelialization and orderly collagen deposition. Furthermore, an integrated approach combining network pharmacology, molecular docking, molecular dynamics simulations, and experimental validation revealed that the therapeutic efficacy of sustained local α-MG delivery involves the modulation of the PPAR-γ pathway. This regulatory process suppresses the NF-κB/COX-2 inflammatory axis and upregulates pro-angiogenic markers, including VEGF, CD31, and α-SMA. Collectively, this dual-action platform, which synergizes physical barrier protection with targeted molecular modulation, represents a promising translational strategy for refractory diabetic wounds. Schematic illustration of the preparation process and functional attributes of the in situ forming CMCSMA/F127/TPGS@α-MG hydrogel. Download: Download high-res image (67KB) Download: Download full-size image
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Journal of Bionic Engineering
·
2026
·
IF 4.4
Hao Jingbin,Shen Ding,Liu Siyu,Wang Shuaihang,Shi Xi,Qiao Yuehua,Naveena Konduru,Zhang Gaorong,Sun He,Zhang Zuoli
Given the poor efficacy of conventional oral medications in treating inner ear disorders due to the presence of the Blood-Labyrinth Barrier (BLB) in the inner ear, we designed an inner ear injectable Andro@GelMA hydrogel for the repair of Diabetic Hearing Loss (DHL). Natural protein-derived materials, such as collagen and gelatin, hold broad prospects in tissue engineering and regenerative medicine. Specifically, Gelatin Methacryloyl Acetate (GelMA), a photosensitive polymer obtained by modifying gelatin with methacrylic anhydride, inherits the high biocompatibility of collagen, making it an ideal drug delivery carrier. In this study, FTIR, XRD, SEM, and rheological tests demonstrated that hydrogen-bonding linkages formed between Andrographolide (Andro) and GelMA, thereby enhancing cross-linking degree and structural stability. Additionally, in vitro and in vivo tests confirmed that the Andro@GelMA hydrogel could achieve controllable sustained release by adjusting the cross-linking degree. Furthermore, in vitro cellular experiments showed strong protective effects on auditory cells, while animal experiments verified that this local delivery system significantly improved the therapeutic efficacy of DHL and promoted the regeneration of damaged inner ear hair cells compared to the control group.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Sheng Ding,Siyu Liu,Shaoyang Kang,Shuwei Wang,Nan Jiang,Yuwei Li,Chunxiao Zhou,Hantao Lou,Chuangnian Zhang,Pingsheng Huang,Wenshuai Liu,Zujian Feng,Weiwei Wang,Deling Kong,Ningbei Yin
Diabetic wound healing poses a major clinical challenge. One of the promising therapy, the flap transplantation surgery exhibited unsatisfactorily low flap survival due to the intertwined pathological barriers: impaired angiogenesis, excessive oxidative stress, and persistent inflammation, leading to poor tissue repair. While the therapeutic platforms based on extracellular vesicles (EVs) emerges as a promising treatment, its efficacy is often limited by the inadequate yield and rapid in vivo clearance. To address this challenge, this study developed a strategy centered on preconditioning human umbilical vein endothelial cells (HUVECs) with high glucose (HG) stress to produce HG-preconditioned extracellular vesicles (hEVs), which significantly improve production, enrich regenerative cargoes (e.g., HIF-1α, VEGF), and enhance homologous cellular internalization. The hEVs derived from HUVECs were encapsulated by microfluidically fabricated gelatin methacryloyl (GelMA) microgels to create GelMA@hEVs. The biodegradable and biocompatible GelMA microgels enabled a sustained hEVs release profile. Therefore, GelMA@hEVs exhibited significant efficacy improvement in promoting endothelial cell proliferation, migration, and tube formation critical for vascularization, mitigating reactive oxygen species, and modulating macrophage polarization toward a pro-reparative phenotype. Mechanistically, these therapeutic effects relied on activating the HIF-1α/VEGF pathway, a core axis for angiogenesis dysregulated in diabetes. In diabetic ischaemic flap models, GelMA@hEVs significantly improved flap survival, restored vascular perfusion, and facilitated tissue regeneration without systemic toxicity. Altogether, this work provides a generalizable strategy for diabetic ischaemic flap repair by combining engineered EVs as bioactive cargo with a microgel scaffold for sustained delivery, offering a promising in situ tissue engineering solution.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yuting Cheng,Daping Yu,Zixun Wang,Bo Zhao,Pengfei Wei,Wei Jing,Yiqian Huang,Yuxin Guo,Huimin Li,Xuening Wang,Peng Jiao,Yandong Li,Yi Han,Chong Wang
Developed a dual-stage sequential curcumin releasing SIS-based self-adhesive hydrogel lung patch. • Achieved strong wet adhesion, suture-free air-leak sealing, and staged anti-inflammatory therapy. • Validated reduced pneumothorax, fibrosis, and inflammatory pleural adhesions in rat and pig pulmonary injury repair models. Postoperative pulmonary air leakage and uncontrolled inflammation remain major challenges after lung resection, while existing patches primarily provide mechanical sealing without active regulation of the healing microenvironment. Here, we report a self-adhesive, dual-stage sequential drug-releasing lung patch (Hydrogel patch–Cur) that integrates rapid sealing with stage-specific immunomodulation. The patch is constructed by coating a decellularized porcine small intestinal submucosa (SIS) scaffold with a bilayer gelma-based hydrogel system, enabling sequential curcumin (Cur) delivery through structurally distinct networks. The outer low-crosslinked gelma30–CS layer enables rapid drug release and enhanced wet-tissue adhesion, while the inner highly crosslinked gelma80 layer provides sustained release for prolonged immune regulation. This design establishes a time-dependent therapeutic profile that matches the dynamic inflammatory phases of lung repair. The patch exhibits strong adhesion (32-fold higher adhesion than uncoated SIS) on wet tissues and effective sealing performance without the need for sutures or staples. In vitro studies demonstrate promoted macrophage polarization toward a pro-regenerative phenotype and significant suppression of pro-inflammatory cytokines. In vivo, the patch rapidly prevents air leakage and pneumothorax, reduces inflammation and fibrosis, and accelerates lung tissue regeneration in both rat and porcine models, outperforming conventional methods and a clinically used self-adhesive patch. This study presents a clinically translatable strategy that combines self-adhesive sealing with gradient drug delivery for improved postoperative lung repair.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Qiong'e Sun,Qiqi Li,Dandan Zhao,Ziyue Li,Yikun Shang,Yu Sun,Meijing Wang,Jiasen Lin,Kun Yu,Xiang Su,Yu Mao,Yan He,Xin Cheng,Xujie Liu
GelMA microneedles enhance the transdermal delivery of PDEVs. • PkEVs were efficiently encapsulated and released from GelMA MNs. • Pk@MN attenuates oxidative stress and ROS in skin cells. • Pk@MN effectively alleviate UVB-induced photoaging in vitro and in vivo . Ultraviolet B (UVB) exposure is a major extrinsic factor inducing skin photoaging, while conventional photoprotective strategies are often limited by insufficient skin penetration and poor compliance. In this study, a methacrylated gelatin (GelMA)-based microneedle system was developed for the efficient transdermal delivery of extracellular vesicles derived from Polygonatum kingianum (PkEVs) to prevent UVB-induced skin photoaging. The PkEVs exhibited a typical spherical morphology, with an average diameter of 168.2 ± 10.1 nm, and were successfully incorporated into a GelMA-based microneedle system (Pk@MN) for transdermal delivery. Pk@MN exhibited sufficient mechanical strength, with a breaking force of approximately 0.36 N per needle, and showed a rapid PkEV release profile, with approximately 90% of PkEVs released within 10 min. In vitro , Pk@MN effectively inhibited UVB-induced oxidative stress and cellular senescence in HaCaT cells, as indicated by reduced intracellular reactive oxygen species (ROS) levels and decreased senescence-associated β-galactosidase (SA-β-Gal) positive cells. Pk@MN also suppressed LPS-induced inflammatory responses in RAW264.7 cells. In vivo , pretreatment with Pk@MN markedly inhibited UVB-induced skin photoaging in mice, maintained skin elasticity by suppressing epidermal thickening, and promote dermal collagen deposition, with a 2.1-fold increase in collagen density compared with the Model group. Moreover, Pk@MN significantly suppressed the expression of the proinflammatory cytokine interleukin-6 (IL-6), approaching the level observed in the Control group. Transcriptome sequencing analysis further suggested that the protective effects of Pk@MN were associated with the regulation of pathways related to inflammation, oxidative stress, and tissue repair. This study highlights a GelMA-based microneedle platform for efficient transdermal delivery of plant-derived extracellular vesicles and provides a promising strategy for preventing UVB-induced skin photoaging.
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Gels
·
2026
·Vol. 12
·期 7
·635
·
IF 6.4
Tongyu Qiu,Fengyuan Bian,Tong Meng,Wei Zhou,Weijie Zhang,Ming Ma,Yihu Wang,Bing Zhang
In this study, taking NdCl3and 2-amino-1,4-benzenedicarboxylic acid (H2ATA) as raw materials, a novel lanthanide metal–organic framework, Nd(ATA), was synthesized by the coprecipitation method. After loading antibiotic levofloxacin (LEV), Nd(ATA) was combined with GelMA hydrogel to prepare a drug-loaded composite hydrogel, LEV@Nd(ATA)-Gel, which can emit near-infrared fluorescence under excitation at 808 nm and possesses improved mechanical properties compared to pure GelMA hydrogel. LEV@Nd(ATA)-Gel exhibited high bactericidal activity and low cytotoxicity, with cell viability increased by 35% compared to the control group. The release rate of the loaded LEV was found increasing with the pH decreasing from 7 to 3, and demonstrated a potential responsiveness to wound microenvironment. Furthermore, drug delivery studies revealed a significant correlation with the fluorescence intensity of the composite hydrogel and the drug release behavior, and the extent of drug release was quantitatively captured by an in vitro imaging technology. This study successfully integrated the drug release with fluorescent signal of carrier, providing a highly sensitive and visualizable strategy for the development of internal wound adhesive.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Zirun Ren,Litao Shao,Yudi Zhang,Bin Di,Mengxiang Su
MAA-MSA functionalized cotton fibers enabled efficient capture of ATS from exhaled breath. • The proposed UHPLC–MS/MS method achieved sensitive detection with an LOQ of 100 pg/pad. • Methamphetamine and amphetamine were successfully monitored in rat breath for up to 5 h. • A low-cost functionalized cotton fiber was developed for capture of amphetamine-type stimulants from exhaled breath. Breath analysis has gained attention as a non-invasive method for drug abuse detection, offering convenience and efficiency compared to traditional blood and urine testing, which often involves invasive sampling and raises privacy concerns. Breath samples provide an advantageous alternative however, the application of breath analysis in drug enforcement is challenged by the rapid loss of breath samples during collection and the ultra-low concentrations of analytes, both of which complicate effective sampling and sensitive detection. In this study, a cost-effective functionalized cotton fiber material modified with methacrylic anhydride (MAA) and mercaptosuccinic acid (MSA), termed MAA-MSA-CF, was developed for capturing and analyzing the trace amphetamine-type stimulants (ATS) in exhaled breath samples. Experimental results demonstrated that the MAA-MSA-CF achieved excellent capture efficiencies for amphetamine (AMP), methamphetamine (MA), and 3,4-methylenedioxy-methamphetamine (MDA), with rates of 84%, 93%, and 86%, respectively, and allowed sensitive detection with a limit of 100 pg/pad via ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). This method was successfully applied to the capture and analysis of ATS from rat breath samples, highlighting the potential of functionalized cotton fiber as non-invasive platform for drug abuse screening and offering a promising tool for enhanced drug control applications. Download: Download high-res image (186KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Jiayu Cheng,Heng An,Xiangdan Meng,Li-Ping Xu
Foodborne microbial contamination is a major global health concern, with conventional methods often being time-consuming and complex. Herein, we developed a novel portable biosensor by integrating microneedle patch technology and a metal-organic framework (Fe/Cu-NBDC MOF) nanozyme, enabling rapid, on-site, visual detection of bacteria in meat. The sensing system works by encapsulating aptamer-functionalized MOF nanozymes within a hydrogel patch, where their catalytic sites are initially blocked by the aptamer. In the presence of Staphylococcus aureus ( S. aureus ) as the target, the specific aptamer's binding to bacteria exposes numerous catalytic sites, further activating the chromogenic reaction of the tetramethylbenzidine‑hydrogen peroxide (TMB-H₂O₂) system, enabling visual detection of S. aureus . The biosensor demonstrates a detection limit of 82 CFU/mL with excellent specificity to successfully apply to commercial mutton. By integrating sampling, enrichment, and visual detection into a single compact device, this platform offers a practical, efficient solution for rapid on-site screening of foodborne pathogens.
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Advanced Science
·
2026
·
IF 14.1
Delu Zhao,Qiuyi Li,Lingyun Wang,Chongao Zhang,Yutian Cheng,Shuang Liu,Fulan Wei,Zheqin Dong
Early mechanical stabilization within the osteogenic niche is a key regulator of bone regeneration fate, and maintaining stability in material-driven repair remains a clinical challenge in dynamic, non-retentive, and anatomically complex bone defects, where implant materials are difficult to achieve stable fixation. However, most existing repair systems lack satisfactory strategies for addressing it. Here, this study reports an interfacial-fixation–centered scaffold design realized by a bioadhesive-integrated multilayer architecture fabricated via 3D-printing, comprising a soft-tissue–blocking barrier layer, a wet-adhesive interface, and a porous osteogenic framework. The scaffold exhibits rapid and robust adhesion to bone under blood-rich physiological conditions, enabling immediate stabilization of the osteogenic niche. In rabbit mandibular and tibial augmentation models, it significantly outperforms non-adhesive scaffolds and the specific guided-bone-regeneration-like comparator. Mechanistically, adhesive-mediated stabilization reduced scaffold micromotion and was associated with a pro-regenerative mechano-immune profile, including increased M2-like macrophage polarization and enhanced VEGF/BMP2-associated angiogenic–osteogenic coupling. By highlighting the interface-fixation-mediated mechanical stabilization of the osteogenic niche, this work offers a new framework for enhancing bone augmentation in dynamic and complex defect settings, provides insights into potential mechano-immune cues for mechanical-stability-secured bone augmentation, and demonstrates the scaffold's potential in bone repair.
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CELL AND TISSUE BANKING
·
2026
·Vol. 27
·期 3
·45
·
IF 2.3
Yuan Yijun,Luo Xiaojing,Tang Longyu,He Tianwen,Zou Xinru,Feng Lu,Su Bin,Wu Jia,Li Jun,Xiao Dongqin
Ovarian cancer (OC) is one of the most aggressive malignancies, with high mortality rates largely attributed to late-stage diagnosis, tumor heterogeneity, recurrence, and resistance to chemotherapy. Artesunate (ART), a water-soluble derivative traditionally used to treat malaria, has recently gained attention as a promising anticancer agent. Recently, studies have shown that ART can inhibit the migration and invasion of cancer cells. However, theses studies are always based on conventional two-dimensional (2D) culture models, which neglect the complex interactions between cells and the extracellular matrix (ECM) or in vivo microenvironment. To address these challenges, in this study, honeycomb-like gelatin methacryloyl (GelMA) hydrogel microspheres as a three-dimensional (3D) culture platform were introduced to mimic the native ECM microenvironment and assess the effects of ART on ovarian cancer cells. The results showed that these GelMA microspheres exhibited a uniform and highly porous structure, which supported cell adhesion, proliferation, and migration. Notably, 3D culture promoted DNA synthesis and induced G1-to-S phase transition, indicating enhanced proliferative capacity. Furthermore, ovarian cancer cells cultured in 3D exhibited stronger invasive and chemoresistant phenotypes. RNA sequencing revealed that after ART treatment, tumor-related proliferation and invasion genes of ovarian cancer cells were significantly up-regulated under 3D culture compared with 2D culture. This might be related to the up-regulation of TNF signaling pathway, NF-kappa B signaling pathway, and Pl3K-Akt signaling pathway. Correspondly, epithelial-mesenchymal transition (EMT) of ovarian cancer cells was markedly enhanced when cultured on GelMA hydrogel microspheres after ART treatment. These effects might be attributed to the altered microenvironment provided by the GelMA hydrogel microspheres, which could be responsible for the enhanced resistance of ovarian cancer cells to ART. These findings suggest that, compared to traditional 2D. These findings suggest that, compared to traditional 2D culture, the 3D extracellular matrix-based system better mimics the in vivo tumor architecture, thereby improving the sensitivity of anticancer drug screening and offering a more reliable platform for evaluating drug resistance and tumor cell heterogeneity.
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