ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Yang Xiaoying,Liu Qian,Luo Longhui,Tian Wei,Kang Chao,Yang Wanliang,Li Tianxiang,Chen Dongmei,Yan Xiufang
A novel biosensing strategy based on the synergy of aptamer molecular recognition and surface-enhanced Raman spectroscopy (SERS) has been developed to address the growing problem of groundwater and soil contamination by the organophosphorus pesticide diazinon (DZN). By combining a high-affinity aptamer with a plasmonic resonance-enhanced substrate, a SERS biosensing interface with single-molecule detection capability was successfully constructed, and the electrochemical surface-enhanced Raman spectroscopy (EC-SERS) method using AgNPs-modified screen-printed electrodes (SPEs) was also used for sensitive detection of DZN. The results showed that the constructed SERS aptamer sensor was able to specifically identify diazinon in the system where multiple interfering pesticides coexisted, and the detection limit of the sensor for diazinon was up to 5.33 × 10 −10 M. The intensity of the characteristic peaks (602 cm −1 ) showed a good linear response with the concentration of DZN, which demonstrated very high detection sensitivity and specific identification function. The proposed EC-SERS method significantly improves the SERS signals of pesticides by potentiometrically modulating the plasmonic resonance coupling effect at the interface of AgNPs-modified electrodes, inducing an orientationally regularised arrangement of DZN molecules. The SERS aptasensor was used to detect diazinon in wastewater and subsurface soil with good recoveries (83.20%–117.78%), which were in good agreement with the results obtained by high-performance liquid chromatography. The results demonstrated that the SERS aptasensor has good sensitivity, stability, simplicity of operation, and specific identification, which provides a solution with ultra-sensitive response, rapid analysis, and strong anti-interference for the detection of trace pesticide.
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PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 4
·1452-1466
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IF 4.462
Pei-Rong Li,Yu Shi,Di Ju,Yu-Xi Liu,Wei Wang,Ying-Shi He,Yu-Yun Zhang,Xue-Qing Yang
BACKGROUND The evolution of insect resistance to pesticides poses a continuing threat to sustainable pest management. While much is known about the molecular mechanisms that confer resistance in model insects and few agricultural pests, far less is known about fruit pests. Field-evolved resistance to synthetic insecticides such as lambda -cyhalothrin has been widely documented in Cydia pomonella , a major invasive pest of pome fruit worldwide, and the increased production of cytochrome P450 monooxygenases (P450s) has been linked to resistance in field-evolved resistant populations. However, the underlying molecular mechanisms of P450-mediated insecticide resistance remain largely unknown . RESULTS Here we found that functional redundancy and preference of metabolism by P450s genes in the CYP9A subfamily confer resistance to lambda -cyhalothrin in Cydia pomonella . A total of four CYP9A genes, including CYP9A61 , CYP9A120 , CYP9A121 , and CYP9A122 , were identified from Cydia pomonella . Among these, CYP9A120 , CYP9A121 , and CYP9A122 were predominantly expressed in the midgut of larvae. The expression levels of these P450 genes were significantly induced by a lethal dose that would kill 10% (LD 10 ) of lambda -cyhalothrin and were overexpressed in a field-evolved lambda -cyhalothrin resistant population. Knockdown of CYP9A120 and CYP9A121 by RNA-mediated interference (RNAi) increased the susceptibility of larvae to lambda -cyhalothrin. In vitro assays demonstrated that recombinant P450s expressed in Sf9 cells can metabolize lambda -cyhalothrin, but with functional redundancy and divergence through regioselectivity of metabolism. CYP9A121 preferred to convert lambda -cyhalothrin to 2′-hydroxy- lambda -cyhalothrin, whereas CYP9A122 only generated 4′-hydroxy metabolite of lambda -cyhalothrin. Although possesses a relatively low metabolic capability, CYP9A120 balanced catalytic competence to generate both 2′- and 4′-metabolites. CONCLUSION Collectively, these results reveal that metabolic functional redundancy of three members of the CYP9A subfamily leads to P450-mediated lambda -cyhalothrin resistance in Cydia pomonella , thus representing a potential adaptive evolutionary strategy during its worldwide expansion. © 2022 Society of Chemical Industry.
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高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Mei-jun Chen,Hai-lin Yang,Ya-min Si,Qian Tang,Cheuk-fai Chow,Cheng-bin Gong
A visible-light-responsive azobenzene derivative, 3,5-dichloro-4-((2,6-dichloro-4-(methacryloyloxy)phenyl)diazenyl)benzoic acid, was synthesized and used as the functional monomer to fabricate a visible-light-responsive core-shell structured surface molecularly imprinted polymer (PS-co-PMAA@VSMIP). After removal of the sacrificial PS-co-PMAA core, a hollow structured surface molecularly imprinted polymer (HVSMIP) was obtained. Both the PS-co-PMAA@VSMIP and HVSMIP were used for the detection of chlorpyrifos, a moderately toxic organophosphate pesticide. They exhibited good visible-light-responsive properties (550 nm for trans→cis and 440 nm for cis→trans isomerization for an azobenzene chromophore) in ethanol/water (9:1, v/v). Compared with the PS-co-PMAA@VSMIP, the HVSMIP had a larger surface area, pore volume, binding capacity, imprinting effect, maximum chemical binding capacity, dissociation constant, and photo-isomerization rate. The HVSMIP was applied to detect trace chlorpyrifos in fruit and vegetable samples. This was achieved by measuring the trans→cis rate constant of the HVSMIP in the sample solution, with good recoveries, low relative standard deviations, and a low detection limit.
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高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 9
·1938-1948
·
IF 2.413
Liang Ting,Gao Lei,Qin Dongli,Chen Ligang
A method for determination of sulfonylurea herbicides (SUHs) in grain was developed by using mesoporous structured molecularly imprinted polymers (M-MIPs) as adsorbents of matrix solid-phase dispersion (MSPD) extraction, coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this method, M-MIPs were synthesized by selecting chlorimuron-ethyl as template molecule, cetyltrimethylammonium bromide as mesoporous structure directing agent, 3-aminopropyltriethoxysilane as functional monomer, and tetraethyl orthosilicate as crosslinker. Then, M-MIPs were characterized by diverse instrumental techniques, absorption characteristics of M-MIPs were studied, and the experimental conditions for the MSPD extraction were optimized. MSPD enabled extraction and clean-up steps to be completed simultaneously. The M-MIPs with mesoporous structure improved detection selectivity, increased adsorptive capacity, and accelerated adsorption rate. Under the best conditions, excellent linearity of SUHs was obtained in the range of 0.25–25 μg kg −1 , the determination limits of chlorimuron-ethyl, nicosulfuron, thifensulfuron-methyl and chlorsulfuron were 0.075, 0.069, 0.083, and 0.105 μg kg −1 , respectively. The relative standard deviations were less than 5.5%.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Yonghui Nie,Yuanjie Teng,Pan Li,Wenhan Liu,Qianwei Shi,Yuchao Zhang
A novel label-free aptamer surface-enhanced Raman scattering (SERS) sensor for trace malathion residue detection was proposed. In this process, the binding of malathion molecule with aptamer is identified directly. The silver nanoparticles modified with positively charged spermine served as enhancing and capture reagents for the negatively charged aptamer. Then, the silver nanoparticles modified by aptamer were used to specifically capture the malathion. The SERS background spectra of spermine, aptamer, and malathion were recorded and distinguished with the spectrum of malathion–aptamer. To enhance the characteristic peak signal of malathion captured by the aptamer, the aggregate reagents (NaCl, KCl, MgCl 2 ) were compared and selected. The selectivity of this method was verified in the mixed-pesticide standard solution, which included malathion, phosmet , chlorpyrifos-methyl, and fethion. Results show that malathion can be specifically identified when the mixed-pesticide interferences existed. The standard curve was established, presenting a good linear range of 5 × 10 − 7 to 1 × 10 − 5 mol·L − 1 . The spiked experiments for tap water show good recoveries from 87.4% to 110.5% with a relative standard deviation of less than 4.22%. Therefore, the proposed label-free aptamer SERS sensor is convenient, specifically detects trace malathion residues, and can be applied for qualitative and quantitative analysis of other pesticides.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Le-Yu Huang,Huiling Wang,Yue-Yang Zhang,Shi-Yu Liu
Due to increasing threats to global public health and widespread environmental pollution issues caused by improper and excessive application of pesticides, the detection of pesticide residues is important in securing food safety and responding to public health. However, conventional methods for pesticide residues detection were usually labor- and time-consuming, making the acquisition of efficient tools for rapid and sensitive detection of pesticide residues an urgent need. Enzyme-targeted organic fluorescent probes, which displayed high simplicity and sensitivity, have shown great potential in enzyme inhibition-based pesticide residues detection and related bioimaging. Among these, fluorescent probes-based biosensors for pesticide targeted serine hydrolyses such as carboxylesterases and cholinesterases have been widely developed. Acylaminoacyl-peptidase hydrolase (APEH), a serine hydrolase with typical α/β fold structure, is a promising protein target for pesticides such as organophosphorus (OPs) and carbamate. However, no fluorescent probe targeting APEH has been reported for pesticide detection or related research. To address this, an enzyme-activated fluorescent probe (named as RH-AcA ) with high sensitivity (limit of detection = 3.7 mU/mL), binding affinity ( K m = 6.49 ± 0.29 μM) and high specificity toward APEH was constructed whilst inhibitory efficacy of different pesticides toward APEH in living cells and zebrafish was first visualized. Most importantly, APEH-inhibition-based pesticide residues detection was first achieved using RH-AcA , revealing significantly higher detection sensitivity toward OPs compared to esterase-based fluorescent probes This demonstrated APEH is a promising non-esterase target for enzyme-inhibition-based pesticide residues detection, and RH-AcA could serve as an ultrasensitive and practical tool for pesticides detection and related bioimaging.
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高影响因子
危险品化学品经营许可证（带存储）