JOURNAL OF CHROMATOGRAPHIC SCIENCE
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2020
·Vol. 58
·期 9
·880-886
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IF 1.28
Li Xiao,Yuan Tao,Zhao Ting,Wu Xiaomei,Yang Yaling
An environmentally friendly method for the determination of testosterone and methyltestosterone by acid–base-induced deep eutectic solvents liquid–liquid microextraction (DES-ABLLME) combining with high-performance liquid chromatography was established. The deep eutectic solvent (DES) consisting of menthol:lauric acid:decanoic acid (3:1:1) can act as both hydrogen bond donor and hydrogen bond acceptor. In this approach, ammonia solution (NH3•H2O) is used as an emulsifier to react with DESs in the extraction process to generate salt and form milky white solution, achieving high extraction efficiency. Hydrochloric acid was used as a phase separator to change the emulsification state and promote the separation of extraction agent from water phase. A series of parameters were optimized including the volume of DES and the emulsifying agent, glucose concentration as well as hydrochloric acid volume. The method was linear in the range 0.5–100 μg mL−1 with a correlation coefficient (R) of 0.9999, and the limits of detection were 0.067 and 0.2 μg mL−1 for testosterone and methyltestosterone, respectively. This method was applied to analyze testosterone and methyltestosterone in milk samples, and the recoveries were between 89.2 and 108.2%.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 16
·期 1
·114-121
·
IF 3.1
Liufeng Zhang,Juan Sun,Yinmeng Ding,Li Li,Jing Liu
Methyltestosterone is one of the banned drugs in aquaculture, and it should be monitored in food-producing animals. 17α-Methyl-5β-androstane-3α,17β-diol, as the main metabolite of methyltestosterone in vertebrates, could be used as another marker for controlling the administration of methyltestosterone, due to its high residual concentration and slow elimination rate. In this study, an analytical method based on gas chromatography-mass spectrometry (GC-MS) was developed and validated for the simultaneous determination of methyltestosterone and its main metabolite in fish. After pretreatment by liquid–liquid extraction with n-hexane and solid phase extraction with C18 and NH2 columns, the target analytes in the muscle tissues were extracted and concentrated, and the influence of the sample matrix was eliminated. Then, the prepared samples were separated and detected with GC-MS in the selected ion monitoring (SIM) mode. Methyltestosterone-D3 was chosen as the internal standard for quantitation. After optimization, the limits of detection for methyltestosterone and 17α-methyl-5β-androstane-3α,17β-diol were 20 μg kg−1 and 15 μg kg−1, respectively. The limits of quantitation were both 50 μg kg−1. The calibration curves showed good linearity in the concentration range from 50.0 ng mL−1 to 500.0 ng mL−1. The correlation coefficients of methyltestosterone and 17α-methyl-5β-androstane-3α,17β-diol were more than 0.9990. The recoveries of the analytes in real samples were in the range of 99.7–116.6% with the relative standard deviation of 5.2–8.3%. The established method could meet the demand for simultaneous detection of methyltestosterone and its major metabolite, and it could be used to provide more information on the abuse of methyltestosterone in food-producing animals.
研究领域:
分析化学
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水产养殖
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食品安全
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DOI
PubMed
高影响因子
Animals
·
2025
·Vol. 15
·期 6
·870
·
IF 2.7
Bai Liufu,Qiyao Su,Kunhao Hong,Jie Wei,Yakun Wang,Zhiqiang Han,Lingyun Yu
Simple Summary17α-methyltestosterone (MT) is known to suppress ovaries and induce spermatogenesis; yet, its effects in crustaceans remain underexplored. This study investigates the impact of dietary MT on gonad development and intestinal microbiota in juvenileMacrobrachium rosenbergiithrough sex ratio determination, histological observation, the expression of reproductive development-related genes, and an analysis of the intestinal microbiota structure. The results showed that an. optimal MT concentration and treatment duration promoted gonadal differentiation and growth. However, excessive MT concentrations inhibited male gonad development and slowed growth rates. MT supplementation significantly increased microbial abundance and altered the intestinal microbial community structure. These findings clarify the effects of adding MT to feed on the reproductive development and intestinal microbiota of juvenileM. rosenbergii, providing a reference for MT application in crustaceans.17α-methyltestosterone (MT) is known for its ability to suppress ovaries and induce spermatogenesis; yet, its effects in crustaceans are underexplored. This study investigates the impact of varying MT concentrations on the gonadal development and intestinal microbiota of juvenileMacrobrachium rosenbergii. Feeds containing different MT doses were provided, and the sex ratios, histological observations, reproductive gene expression, and intestinal microbial composition were analyzed. The results revealed short-term feeding (60 days) of 1000 mg/kg MT resulted in the highest male ratio, while long-term feeding (150 days) of 500 mg/kg MT achieved the same outcome. Conversely, long-term feeding of 1500 mg/kg MT led to the lowest male ratio and retarded male germ cell development. An intestinal microbiota analysis showed that MT supplementation significantly increased microbial abundance and altered the intestinal microbial community structure. Additionally, MT suppressed the expression of female reproductive-related genes. This study provides insights into the effects of MT on reproductive development and gut microbiota in juvenile prawns, offering a valuable reference for the application of MT in crustacean aquaculture.
研究领域:
水产养殖学
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分子生物学
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微生物学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Yabing Shan,Jiayi Li,Ying Chen,Dongmei Li,Yanbo Luo,Yongqiang Pang,Di Chen
The illegal addition of androgenic steroids, such as testosterone and methyltestosterone, to herbal oral liquids poses a significant threat to consumer health. This study presents a novel method for the extraction and determination of these steroids in herbal products using pipette tip solid-phase microextraction combined with liquid chromatography-tandem mass spectrometry. The pipette tips are coated with hydroxyl-functionalized multi-walled carbon nanotubes, ensuring efficient analyte extraction while reducing sample handling and contamination risks. The method was optimized for parameters such as solvent volume, extraction time, and pH, and demonstrated excellent analytical performance. It demonstrated linearity over the range of 5–5000 ng/mL (R 2 > 0.99), with detection limits of 0.96 ng/mL for testosterone and 1.44 ng/mL for methyltestosterone, and limits of quantification of 3.20 ng/mL for testosterone and 4.80 ng/mL for methyltestosterone. Recovery experiments across multiple matrices and concentration levels showed recoveries ranging from 93.8 % to 108.1 %, with relative standard deviations between 1.1 % and 4.5 %. This approach offers a cost-effective, high-throughput solution for the routine detection of illegal steroid additives in herbal and functional food products, providing a valuable tool for regulatory monitoring and consumer safety.
研究领域:
分析化学
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食品安全
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药物分析
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DOI
高影响因子
International Journal of Electrochemical Science
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2023
·
IF 1.5
Yuzhe Liu,Chunlian Quan
This research paper introduces a highly sensitive electrochemical sensor designed for the detection of the sports stimulant methyltestosterone, utilizing a unique ZnO/TiO 2 nanocomposite. Methyltestosterone, an extensively abused anabolic steroid in sports, calls for an efficient detection technique to maintain sporting integrity. The sensor was designed and synthesized with enhanced properties leveraging the specific characteristics of the ZnO/TiO 2 nanocomposite. The sensor demonstrated high sensitivity and selectivity towards methyltestosterone, outstripping traditional detection techniques. When subjected to real-world scenarios, including testing in synthetic urine samples, the sensor showcased outstanding precision and consistency, making it a robust tool for anti-doping control.
研究领域:
分析化学
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生物医学工程
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运动科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Junxian Zhu,Yongchang Wang,Luo Lei,Chen Chen,Liqin Ji,Jiansong Li,Congcong Wu,Wenjun Yu,Laifu Luo,Weiqin Chen,Pan Liu,Xiaoyou Hong,Xiaoli Liu,Haigang Chen,Chengqing Wei,Xinping Zhu,Wei Li
A feature of the Chinese soft-shelled turtle ( Pelodiscus sinensis ) is seasonal spermatogenesis ; however, the underlying molecular mechanism is not well clarified. Here, we firstly cloned and characterized P. sinensis DKKL1 , and then performed comparative genomic studies, expression analysis, and functional validation. P. sinensis DKKL1 had 2 putative N-glycosylation sites and 16 phosphorylation sites. DKKL1 also had classic transmembrane structures that were extracellularly localized. DKKL1 's genetic distance was close to turtles, followed by amphibians and mammals, but its genetic distance was far from fishes. DKKL1 genes from different species shared distinct genomic characteristics. Meanwhile, they were also relatively conserved among themselves, at least from the perspective of classes. Notably, the transcription factors associated with spermatogenesis were also identified, containing CTCF , EWSR1 , and FOXL2 . DKKL1 exhibited sexually dimorphic expression only in adult gonads, which was significantly higher than that in other somatic tissues ( P < 0.001), and was barely expressed in embryonic gonads. DKKL1 transcripts showed a strong signal in sperm, while faint signals were detected in other male germ cells. DKKL1 in adult testes progressively increased per month ( P < 0.05), displaying a seasonal expression trait. DKKL1 was significantly downregulated in testes cells after the sex hormones (17β-estradiol and 17α-methyltestosterone) and Wnt/β-catenin inhibitor treatment ( P < 0.05). Likewise, the Wnt/β-catenin inhibitor treatment dramatically repressed CTCF, EWSR1, and FOXL2 expression. Conversely, they were markedly upregulated after the 17β-estradiol and 17α-methyltestosterone treatment, suggesting that the three transcription factors might bind to different promoter regions, thereby negatively regulating DKKL1 transcription in response to the changes in the estrogen and androgen pathways, and positively controlling DKKL1 transcription in answer to the alterations in the Wnt/β-catenin pathway. Knockdown of DKKL1 significantly reduced the relative expression of HMGB2 and SPATS1 ( P < 0.01), suggesting that it may be involved in seasonal spermatogenesis of P. sinensis through a positive regulatory interaction with these two genes. Overall, our findings provide novel insights into the genome evolution and potential functions of seasonal spermatogenesis of P. sinensis DKKL1 .
研究领域:
基因组学
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生殖生物学
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分子生物学
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比较基因组学
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PubMed
高影响因子
Genes
·
2024
·Vol. 15
·期 5
·605
·
IF 2.8
Haijun Huang,Yuyan Liu,Qian Wang,Caichao Dong,Le Dong,Jingjing Zhang,Yu Yang,Xiancai Hao,Weijing Li,Ivana F. Rosa,Lucas B. Doretto,Xuebin Cao,Changwei Shao
It is widely known that all-female fish production holds economic value for aquaculture.Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17α-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales ofS. schlegeliifor subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth ofS. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1aandfoxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-β signaling and steroid hormone biosynthesis pathways, indicating its crucial role inS. schlegeliisex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT inS. schlegeliiand offers an initial insight into the underlying mechanism of sexual reversal in this species.
研究领域:
水产养殖
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内分泌学
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分子生物学
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生理学
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PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 24
·9616-9621
·
IF 1.938
Chifang Peng,Xiaohui Duan,Gabriel Wafala Khamba,Zhengjun Xie
Based on the H2O2-mediated generation of gold nanoparticles, a colorimetric catalase nanosensor was developed and utilized as a signal output in a catalase-labelled immunoassay. Methyltestosterone (MT), which is used illegally as a growth promoter during the cattle production cycle, was selected as a small molecule model in this new ELISA development. As a result, a “signal on” plasmonic ELISA for MT was established. This plasmonic ELISA can quantitatively detect MT by spectrophotometry with a 50 times higher sensitivity than the traditional ELISA. In addition, it allows the highly sensitive and easily qualitative detection of MT by the naked eye. This plasmonic direct competitive ELISA can be utilized for developing cost-effective and sensitive ELISA against various small molecules by the naked eye.
研究领域:
分析化学
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纳米技术
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生物传感器
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环境科学
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食品安全
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）