JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2022
·
IF 3.185
Qihao Zhang,Xiaohui Yang,Lin Lin,Shuhong Wu,Ping Wang,Wei Wei,Dongzhi Wei
Melanin has an increasing market demand in cosmetics, food, medicine as well as aerospace due to its unique properties. Heterologous expression of 4-hydroxyphenylpyruvate dioxygenase (HPPD) from the melanin-producing strain Streptomyces fungicidicus NW-EN1 in Escherichia coli shortened the fermentation cycle of melanin. HPPD catalyzed 4-hydrophenylpyruvate (HPP) to form homologous acid (HGA) and finally form melanin. The purified melanin had the highest absorption peak at 460 nm. Fourier transform infrared spectroscopy and scanning electron microscope scanning showed that the pigment had universal characteristic peaks. The presence of HGA, a predictor of pyomelanin, was identified by high-performance liquid chromatography analysis. The recombinant E. coli produced 804.4 ± 5.9 mg/L pyomelanin within 48 h. Metal ions had a great influence on the production of pyomelanin. Pyomelanin was stable in response to light intensity and had a protective effect against bacteria under UV irradiation. Meanwhile, we utilized the chromogenic effect after whole-cell catalysis to reflect the inhibition of the HPPD inhibitors (mesotrione and isoxaflutole) on HPPD by observing the color change. As a rapid method to test the action of inhibitors, this method is expected to be useful for the development of HPPD-inhibiting herbicides.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xian-Xian Ji,Yu-Long Liu,Xin-Yue Chang,Rui-Long Li,Fei Ye,Liu Yang,Ying Fu
Diflubenzuron is widely used as a benzoylurea insecticide, and its impact on human health should not be underestimated. Therefore, the detection of its residues in food and the environment is crucial. In this paper, octahedral Cu-BTB was fabricated using a simple hydrothermal method. It served as a precursor for synthesizing Cu/Cu 2 O/CuO@C with a core–shell structure through annealing, creating an electrochemical sensor for the detection of diflubenzuron. The response of Cu/Cu 2 O/CuO@C/GCE , expressed as Δ I / I 0 exhibited a linear correlation with the logarithm of the diflubenzuron concentration ranging from 1.0 × 10 −4 to 1.0 × 10 −12 mol·L −1 . The limit of detection (LOD) was determined to be 130 fM using differential pulse voltammetry (DPV). The electrochemical sensor demonstrated excellent stability, reproducibility, and anti-interference properties. Moreover, Cu/Cu 2 O/CuO@C/GCE was successfully employed to quantitatively determine diflubenzuron in actual food samples (tomato and cucumber) and environmental samples (Songhua River water, tap water, and local soil) with good recoveries. Finally, the possible mechanism of Cu/Cu 2 O/CuO@C/GCE for monitoring diflubenzuron was thoroughly investigated.
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高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 24
·2946-2954
·
IF 3.1
Xiong Chen,Yingxue Li,Juying Li,Li Cao,Cheng Yao
A ratiometric nitenpyram (NIT) upconversion luminescence sensor UCNPs–PMOF was fabricated from a metal–porphyrin organic framework (PMOF) and pretreated UCNPs. The reaction between NIT and the PMOF releases the H2TCPP (5,10,15,20-tetracarboxyl phenyl) porphyrin ligand, which enhances the absorption of the system at 650 nm, and reduces the upconversion emission intensity of the sensor at 654 nm through a luminescence resonance energy transfer (LRET) mechanism, thus achieving the quantitative detection of NIT. The detection limit was 0.21 μM. Meanwhile, the emission peak of UCNPs–PMOF at 801 nm does not change with the concentration of NIT, and the emission intensity ratio (I654 nm/I801 nm) is used to achieve the ratiometric luminescence detection of NIT, and the detection limit is 0.22 μM. UCNPs–PMOF has good selectivity and anti-interference to NIT. In addition, it has a good recovery rate in actual sample detection, which indicates that it has high practicability and reliability in NIT detection.
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高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Qingyun Ma,Qingqing Li,Jie Wang,Rebecca E. Parales,Lin Li,Zhiyong Ruan
Herbicide mixtures are a new and effective agricultural strategy for managing suppress weed resistance and have been widely used in controlling weeding growth in maize fields. However, the potential ecotoxicological impact of these mixtures on the microbial community structure and function within various root-associated niches, remains inadequately understood. Here, the effects of nicosulfuron , mesotrione and atrazine on soil enzyme activity and microbial community structure and function were investigated when applied alone and in combination. The findings indicated that herbicide mixtures exhibit a prolonged half-life compared to single herbicides. Ecological niches are the major factor influencing the structure and functions of the microbial community, with the rhizosphere exhibiting a more intensive response to herbicide stress. Herbicides significantly inhibited the activities of soil functional enzymes, including dehydrogenase , urease and sucrose in the short-term. Single herbicide did not drastically influence the alpha or beta diversity of the soil bacterial community, but herbicide mixtures significantly increased the richness of the fungal community. Meanwhile, the key functional microbial populations, such as Pseudomonas and Enterobacteriaceae, were significantly altered by herbicide stress. Both individual and combined use of the three herbicides reduced the complexity and stability of the bacterial network but increased the interspecific cooperations of fungal community in the rhizosphere. Moreover, by quantification of residual herbicide concentrations in the soil, we showed that the degradation period of the herbicide mixture was longer than that of single herbicides. Herbicide mixtures increased the contents of NO 3 − -N and NH 4 + -N in the soil in the short-term. Overall, our study provided a comprehensive insight into the response of maize root-associated microbial communities to herbicide mixtures and facilitated the assessment of the ecological risks posed by herbicide mixtures to the agricultural environment from an agricultural sustainability perspective.
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高影响因子
POLYHEDRON
·
2025
·
IF 2.6
Yuan-Zhen Wang,Liu Yang,Jia-Jia Kou,Yu-Qing Zhan,Yi-Lin Niu,Yu-Long Liu
Flumioxazin, a nitrophenyl herbicide widely used in agriculture, poses potential health risks due to its residue in agricultural products. This study elucidates the fabrication of a novel electrochemical sensor, MnO@C , specifically designed for the efficient detection of flumioxazin. The sensor MnO@C was created by annealing Mn-BTC , synthesized via a straightforward solvothermal method. The electrochemical behavior of MnO@C for detecting flumioxazin was assessed through DPV. The findings revealed that MnO@C demonstrated a wide detection range, achieving an exceptionally low LOD at 597 fmol/L for flumioxazin. Moreover, MnO@C exhibited exceptional duplicability, reliability, and anti-interference ability. The sensor MnO@C was also effectively utilized for the quantitative detection of flumioxazin in diverse samples. The results highlight the substantially practical utility of MnO@C in food and environment detection, which provides broad-spectrum environmental resistance and fast, efficient detection for flumioxazin residue. Additionally, a thorough analysis of the mechanism of MnO@C for monitoring flumioxazin was undertaken.
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高影响因子
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