INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Jiahong Lin,Qiujie Lai,Yingju Liu,Shi Chen,Xueyi Le,Xiaohua Zhou
Magnetically retrievable formulations of laccase potentially promising for biomedical and environmental applications were constructed by immobilization of the enzyme on the surface of magnetic nanoparticles functionalized by siloxane layers with active amino and methacrylyol moieties. The functional magnetic particles were prepared using a hydrolytic polycondensation reaction of tetraethoxysilane with 3-methacryloxypropyltrimethoxysilane (MPS). The results indicated that the amounts of laccase loading on the methacrylyol functional magnetic particles were approximately 150 mg/g under the optimum conditions. The stability of the immobilized laccase was highly enhanced against thermal treatments and provided better long-term storage. Compared to free laccase, the immobilized laccase exhibited significantly higher decolourisation efficiency in the decolorization of a representative dye, methyl red, which resulted from the magnetic particles adsorption and enzymatic catalysis. These results show its great potential for industrial applications.
研究领域:
生物技术
·
环境科学
·
医学工程
✓ 已验证
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2014
·Vol. 99
·期 5
·2431-2439
·
IF 3.811
Yu Lei,Zhang Xiao-yu,Xie Tian,Hu Jin-mei,Wang Shi,Li Wen-wei
Reduction of azo dye methyl red coupled with aerobic respiration by growing cultures of Klebsiella oxytoca GS-4-08 was investigated. In liquid media containing dye and 0.6 % glucose in a mineral salts base, 100 mg l −1 of the dye are completely removed in 3 h under shaking conditions. The dye cannot be aerobically decolorized by strain GS-4-08 without extra carbon sources, indicating a co-metabolism process. Higher initial dye concentration prolonged the lag phase of the cell growth, but final cell concentrations of each batches reached a same level with range from 6.3 to 7.6 mg l −1 after the dye adaption period. This strain showed stronger dye tolerance and decolorization ability than many reported strains. Furthermore, a new intracellular oxygen-insensitive azoreductase was isolated from this strain, and the specific activity of enzyme was 0.846 and 0.633 U mg −1 protein in the presence of NADH and NADPH, respectively. N , N dimethyl- p -phenylenediamine and anthranilic acid were stoichiometrically released from MR dye, indicating the breakage of azo bonds accounts for the intracellular decolorization. Combining the characteristics of azoreductase, the stoichiometry of EMP, and TCA cycle, the electron transfer chain theory of aerobic respiration, and the possible mechanism of aerobic respiration coupled with azo reduction by K. oxytoca GS-4-08 are proposed. This study is expected to provide a sound theoretical basis for the development of the K. oxytoca strain in aerobic process for azo dye containing wastewaters.
研究领域:
微生物学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Moustafa Zahran,Shereen Gamal,Magdy A.-H. Zahran,Magdi Abdel Azzem
Herein, we report a new electrochemical sensor based on silver nanoparticles (AgNPs) to quantify methyl red (MR) in drinking water. Firstly, AgNPs were greenly synthesized using mucilage natural polymer for the reduction of Ag + and subsequent stabilization of the formed AgNPs. The formation of AgNPs was confirmed by monitoring their characteristic absorption peak at a wavelength of 425 nm and characteristic oxidation potential at 0.035 V using ultraviolet–visible spectroscopy and square wave voltammetry (SWV), respectively. Transmission electron microscopy also confirmed the synthesis of spherical AgNPs with a size range of 10 to 30 nm, while scanning electron microscopy showed the morphology of glassy carbon electrode (GCE) after modification with AgNPs. Additionally, the electrocatalytic activity of AgNPs/GCE was approved by cyclic voltammetry, electrochemical impedance spectroscopy, and SWV. The highly electrocatalytic mucilage-AgNPs were employed as a GCE modifier to quantify MR under optimized conditions of scan rate (0.1 V/s), amplitude (0.025 V), and pH (6) using SWV. The sensor showed a limit of detection of 1.5 picomolar and high selectivity in the presence of some possible interfering dyes in drinking water sources. Additionally, after spiking the drinking water samples with MR, the sensor showed acceptable recovery values.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Nan Li,Lanlan Huo,Wenjing Shen,Chenghong Qiang,Mianmian Wu,Guifang Sun,Qingfei Li,Minghao Shi,Jiangquan Ma
Environmental pollution and energy shortage are key problems restricting human and social development. Finding a way to effectively adsorb organic pollutants in wastewater is of great significance for environmental protection. Here, we reasonably design and prepare three novel porous organic polymers with different ratios m-terphenyl (POP-Ts) by Friedel-Crafts alkylation as excellent adsorbents, named POP-T (1:1), POP-T (1:1.5) and POP-T (1:2), respectively. Notably, the proportion of m-terphenyl and biphenyl dichlorobenzyl was optimized for the first time to obtain POPs adsorbents for removal of pollutants from water. Specially, the adsorption experiments showed that POP-T (1:1.5) has excellent adsorption performance of rhodamine B (RhB), methylene blue (MB) and diclofenac (DCF), and the maximum adsorption of POP-T (1:1.5) is 1414.4 mg/g, 1256.6 mg/g and 1028.9 mg/g, respectively. The kinetic data and isothermal data were well fit with the pseudo 2nd order model and Langmuir model, respectively. Moreover, all POP-Ts exhibit excellent stability and the adsorption efficiency is still over 90% after four cycles. Overall, the superefficient adsorption performance is attributed to the hydrophobicity property, large specific surface area (1416 m 2 /g) and porosity of the POP-T (1:1.5) material.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2013
·Vol. 11
·期 45
·7931-7937
·
IF 3.568
Xinhua Cao,Aiping Gao,Haiting Lv,Yan Wu,Xiuxiu Wang,Yang Fan
A new gelator 1 that included m-methyl red was synthesized and fully characterized. It was found that the organogel of 1 was stable in DMSO even if the organogel was stimulated by acid or light. The organogel was thoroughly characterized using various microscopic techniques including field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), UV-vis and Fourier transform infrared (FTIR) spectra. The organogel exhibited tunable structures and optical properties under different stimulation. The regular nanoring structure was turned into microspheres after the organogel in DMSO was stimulated at 254 nm light or acid. At the same time, the color of molecule 1 in gel state and solution state was all changed after stimulation by light or acid. The hydrogen bonding and π–π stacking were found to be the main driving forces for gel formation. This dual-responsive gel held promise for soft materials application in upscale smart responsive materials.
研究领域:
材料化学
·
软物质科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2021
·
IF 6.064
Mengqi Wang,Kaiyue Zhang,Feixiang Ji,Yurong Guo,Chao Wang,Shiping Wang,Ya Chu,Guangjiu Zhao
A water-soluble probe composite material (CDs/ZnO) was synthesized by microwave pyrolysis methods of citric acid , urea and zinc oxide (ZnO) with aggregation-induced emission activity. We creatively realized the composite of CDs and nano-ZnO in the form of foam, then used as bactericide for the first time and showed excellent antibacterial properties. Also, the composite materials overcame the problem of aggregation-induced quenching (ACQ) and realized solid-state emission. The materials had two different emission centers, and the two emission peaks were varied with the change of excitation wavelength. Furthermore, Methyl red (MR) showed fluorescence quenching for CDs/ZnO with obvious linear correlation, which demonstrated potential in the measurement of MR in dyeing wastewater. The CDs/ZnO we obtained perfectly inherited the advantages of CDs and nano ZnO , it has not just no cytotoxicity to cells, but also has a good killing effect on bacteria. Hence, this work shows significant adaptable fluorescence-based protocol with enormous potential applications in biology.
研究领域:
材料科学与工程
·
生物学
·
环境科学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Hanhan Huang,Caifeng Xia,Derui Liang,Zhaosong Li,Haiyan Wang,Zhiwen Dou,Junshan Yang,Shifeng Zhao,Mingxiu Tang,Qian Zhang,Zilin Meng
A comparative study of anionic contaminants removal using layered double hydroxides (LDHs) by three different paths (ion-exchange, reconstruction and one-pot method) was investigated. The removal of targeted contaminants methyl orange (MO) and methyl red (MR) with similar structure from wastewater by these three methods were tested, respectively. The results of SEM revealed that LDHs obtained by the three methods showed different layered morphologies and the concentration of contaminant almost has no influence to the morphologies. In kinetic study , the removal behaviors of three methods were well described by pseudo-second-order model, and showed different patterns in intraparticle diffusion model , revealing that accessible active sites on the adsorbent were the main factor for different adsorption capacity of these methods. The isotherm experiments manifested that all removal data were well fitted by Langmuir models and revealed a charge-limited process. The adsorption capacity of MO was higher than that of MR by all these methods, and the one pot method had the largest adsorption capacity for MO and MR, which were 2.87 and 2.25 mmol/g respectively. The obtained samples were characterized by XRD and different interlayer spacings were observed by these three methods, which was caused by the amount and the arrangement of MO or MR. Molecular simulation was selected to calculate the change of chemical bond, electron transfer and bonding energy, explaining the different removal capacity of MO and MR.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shuo Sun,Xingyu Fu,Xu Yang,Shuhui Zhao,Huitian Zhi,Ruhui Shang,Xujie Wang,Yingchao Yang,Zhengquan Yan,Lei Hu
To high-performance recognize or degrade o/p -aminophenol and methyl red, nano Zr/Cu oxide decorated poly-β-cyclodextrin (CuO/ZrO 2 @p-β-CD) was developed under microwave-assisted conditions. Fibrous CuO/ZrO 2 @p-β-CD expressed synergistic peroxidase-like activity to degrade >90.4 % methyl red in the air. When applied to accelerate the oxidization of 2,2′-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) ammonium salt (ABTS), K m / V max could be 0.053 mM/2.92 × 10 −8 M/s. Trace aminophenol isomers ( o -AP and m -AP) could exclusively alter CuO/ZrO 2 @p-β-CD-ABTS from green to colorless or to purple-red with remarkable time-resolved effect. Under the optimized conditions, CuO/ZrO 2 @p-β-CD was successfully applied for intelligent monitoring or degradation o -AP, m -AP or methyl red in practice with the help of a smartphone. The recoveries for UV–vis spectral/RGB detection of o -AP and m -AP could reach 100.0 % ~ 102.9 %/98.0 % ~ 102.5 % and 97.8 % ~ 101.1 %/96.8 % ~ 103.33 %. The catalytic detection or quantitative degradation mechanisms were investigated in detail. This work will provide a potential for quantitative monitoring or degradation of organic pollutions.
研究领域:
生物化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 58
·35164-35173
·
IF 3.119
Juan Pang,Xincheng Mao,Jialin Xu,Xiang Zhao,Jingyang Kong,Xiaohong Hu
The application of azobenzene (AZO) as a kind of photo-switch is restricted by its excitation source, i.e., UV light. Hence, visible light-driven azobenzene-based photo-switching is needed and has been designed in the work. In order to forecast the optimal triggered wavelength, the electrostatic potential, theoretical UV-vis spectra, as well as the energy gap for focused structures was calculated to describe the energy and orbit status of the molecules by DFT. According to the theoretical optimization results, m-Methyl Red (m-MR) containing copolymer was successfully synthesized as a visible light-driven photo-switch. Further, for performance evaluation, the efficiency and effectiveness of different excitation wavelengths was firstly evaluated for the copolymer using m-MR and m-Methyl Red acrylic anhydride (m-MRAA) as the controls. Compared with m-MR and m-MRAA, the copolymer exhibited outstanding characteristics as a photo-switch according to its response–recovery behavior. At the same time, blue light proved to be the most efficient excitation light source. Moreover, the equilibrium response time and recovery time showed some dependence on the excitation wavelength. Secondly, the influence of the light intensity on the isomerization transition was investigated. A relatively low light density could lead to a relatively low degree of the final cis form and needed more equilibrium time for trans to cis transformation but showed little effect on the recovery process. Thirdly, repeatable on/off irradiation was used to evaluate the fatigue resistance of the copolymer. Good fatigue resistance without photobleaching was verified from the results. Fourthly, the influence of the solvent on visible light-driven isomerization was also evaluated. Finally, the synthesized copolymer still had the characteristic of a pH indicator with a critical point at pH 5.0 and exhibited an obvious fluorescent characteristic.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2022
·
IF 8.907
Hualiang Xu,Liang Jiang,Anqian Yuan,Zhengkai Wei,Yuan Lei,Yuechuan Wang,Weibo Kong,Xiaowei Fu,Jingxin Lei
Phase change materials (PCMs) are extensively applied in thermal energy management in the recent years due to high latent heat and efficient energy utilization. However, the current studies of PCMs are mainly focused on the single function of thermal storage which greatly limits their advanced applications. Herein, we prepared novel reversible thermochromic phase change materials (TCFPs) consisting of thermochromic compound and filter paper via vacuum-assisted impregnation method. Based on the excellent reversible thermochromic ability, these designed TCFPs can achieve multiple functions, including temperature-controlled information storage and self-reporting along with the thermal storage ability. Taking advantage of the lightweight and flexibility of TCFPs can rapidly create arbitrary shapes, such as the quick response codes, achieving the concealment or exhibition of the information by thermal stimuli. Moreover, TCFPs modified by methyl red can possess a better visual effect and can helps to realize the temperature-controlled self-reporting. Our work demonstrates a facile design strategy of multifunctional TCFPs, unlocking opportunities for smart applications of PCMs.
研究领域:
材料科学
·
热工程
·
信息存储
·
智能材料
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 10
·6867-6880
·
IF 3.612
Qiushi Tang,Jiwen Hu,Shi Li,Shudong Lin,Yuanyuan Tu,Xuefeng Gui
Summary A colorimetric pH-sensitive hydrogel indicator integrated with the smartphone for real-time and visual food spoilage monitoring was developed. Herein, the poly(vinyl alcohol)/sodium alginate (PVA/SA) hydrogel was used as the carriers for the pH-sensitive mixed-dye methyl red/bromothymol blue (MR/BB), which can be employed as an amine indicator. The functional hydrogel was prepared by a simple freezing/thawing process that mainly performed in PVA and calcium cross-linking in SA. The optimal condition for the sensitivity of hydrogel towards ammonia and the structure of the hydrogel were investigated. The potential applications of the hydrogels in beef, fish, chicken and pork were evaluated under different storage conditions. Sigmoidal fitting results indicated that the RGB model with a smartphone can form a strong correlation with the total volatile basic nitrogen (TVB-N) values in common meat products. The hydrogel indicators with sensitive and stable properties have proven to be promising materials for use in food spoilage monitoring.
研究领域:
食品科学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Peng Lu,Yang Yang,Ren Liu,Xin Liu,Jinxia Ma,Min Wu,Shuangfei Wang
A sugarcane bagasse nanocellulose-based hydrogel was developed as a colourimetric freshness indicator for monitoring spoilage of chicken breast. Nanocellulose was prepared from sugarcane bagasse cellulose filaments by TEMPO-mediated oxidation, and then used to form a strong self-standing hydrogel matrix through Zn 2+ cross-linking. The hydrogel worked as a carrier for pH-responsive dyes (bromothymol blue/methyl red), which changed colour according to the freshness of the chicken sample. CO 2 levels were found to increase with the spoilage of chicken, relative to rising levels of micro-organisms. The indicator hydrogel optical colour changed from green to red on the third day, as the log CFU/g passed the limit of acceptability for human consumption and emission of complicated volatiles. This new design of colourimetric freshness indicator made with a nanocellulose hydrogel has a quick response to chicken spoilage and is expected to facilitate the utilisation of bagasse nanocellulose as a value-added material in intelligent packaging.
研究领域:
材料科学
·
食品科学
·
纳米技术
·
包装工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）