JOURNAL OF MEDICAL VIROLOGY
·
2024
·Vol. 96
·期 6
·e29723
·
IF 6.8
Dekai Zheng,Changhao Cheng,Yanhua Tang,Zhixin Fang,Xuelian Gao,Yuchuan Chen,Qiuhong You,Kaifeng Wang,Heqi Zhou,Zhixian Lan,Jian Sun
Hepatitis B virus (HBV) can be completely suppressed after antiviral treatment; however, some patients with chronic hepatitis B (CHB) exhibit elevated alanine aminotransferase (ALT) levels and sustained disease progression. This study provides novel insights into the mechanism and potential predictive biomarkers of persistently elevated ALT (PeALT) in patients with CHB after complete viral inhibition. Patients having CHB with undetectable HBV DNA at least 12 months after antiviral treatment were enrolled from a prospective, observational cohort. Patients with PeALT and persistently normal ALT (PnALT) were matched 1:1 using propensity score matching. Correlations between plasma metabolites and the risk of elevated ALT were examined using multivariate logistic regression. A mouse model of carbon tetrachloride-induced liver injury was established to validate the effect of key differential metabolites on liver injury. Of the 1238 patients with CHB who achieved complete viral suppression, 40 (3.23%) had PeALT levels during follow-up (median follow-up: 2.42 years). Additionally, 40 patients with PnALT levels were matched as controls. Ser-Phe-Ala, Lys-Ala-Leu-Glu, 3-methylhippuric acid, 3-methylxanthine, and 7-methylxanthine were identified as critical differential metabolites between the two groups and independently associated with PeALT risk. Ser-Phe-Ala and Lys-Ala-Leu-Glu levels could be used to discriminate patients with PeALT from those with PnALT. Furthermore, N -acetyl- l -methionine (NALM) demonstrated the strongest negative correlation with ALT levels. NALM supplementation alleviated liver injury and hepatic necrosis induced by carbon tetrachloride in mice. Changes in circulating metabolites may contribute to PeALT levels in patients with CHB who have achieved complete viral suppression after antiviral treatment.
研究领域:
肝病学
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代谢组学
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药理学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2012
·
IF 3.898
Xianfeng Wang,Yang Si,Jialin Wang,Bin Ding,Jianyong Yu,Salem S. Al-Deyab
A novel strategy for highly sensitive colorimetric detection of gaseous formaldehyde was developed based on Methyl Yellow-impregnated electro-spinning/netting (ESN) nylon 6 nano-fiber/nets (NFN). The sensor presented a significant reflectance intensity decreasing band at 550 nm which induce the visual color changes from yellow to red after exposure to formaldehyde and allowed for the detection of formaldehyde with a low detection limit of 50 ppb. Upon exposure to a series of volatile organic compounds (VOCs), only formaldehyde could induce a yellow-to-red color change observable by the naked eye, which clearly exhibited that Methyl Yellow-impregnated nylon 6 NFN membranes could act as highly selective and sensitive strips to detect formaldehyde with minor interference from other VOCs. Additionally, the colorimetric sensor showed good reproducibility under cyclic sensing experiments. Furthermore, the colorimetric responses were visualized quantitative by using a color-differentiation map prepared form converted RGB (red, green and blue) values. As-prepared Methyl Yellow-impregnated nylon 6 NFN sensor strips successfully combined with the visualized detection of color map and fascinating structure of NFN membranes, which indicated promising composite materials as a simple and economical alternative to replace the traditional formaldehyde sensors and facilitated the design and development of other label-free colorimetric sensors toward various analytes based on the NFN template.
研究领域:
材料科学
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环境科学
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化学传感
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yan Li,Lingying Xie,Jingyi Liu,Sisi Tang,Hang Lei,Yuxia Zhong,Yue-Fei Zhang
In this study, an imidazole cross-linked chitosan hydrogel (I/CS-4) was synthesized through the Debus-Radziszewski reaction, resulting in a multifunctional adsorbent with both dye adsorption and antimicrobial capabilities. I/CS-4 effectively removed orange G (OG) and metanil yellow (MY) dyes under various environmental conditions, including pH, initial concentration, temperature, and contact time. The hydrogel demonstrated high stability and efficiency over a broad pH range (2−10), attributed to its abundant amino and imidazole groups, which facilitate electrostatic, hydrogen bonding, and π-π stacking interactions. The maximum adsorption capacities for OG and MY were 565.00 mg/g and 490.20 mg/g, respectively, following the Langmuir isotherm and pseudo-second-order kinetic models. Additionally, I/CS-4 showed excellent recyclability, maintaining over 80 % removal efficiency after five cycles. It also exhibited potent antibacterial activity against E. coli and S. aureus while being biocompatible. These results highlight I/CS-4's potential as an effective material for treating dye-containing wastewater, combining efficient contaminant removal with microbial inhibition.
研究领域:
环境科学
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材料科学
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生物学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Ziteng Wang,Yanjuan Peng,Xinxin Cheng,Jingjing Yang,Bin Zhou,Li Mi,Yonghong Hu
In this work, the dissolution behavior of a novel pharmacological factor, 3-methylflavone-8-carboxylic acid (MFCA) in different solvents was studied. Firstly, the solubility of MFCA in ten mono-solvents (acetonitrile, tetrahydrofuran, acetone, N,N-dimethylformamide, methyl acetate, ethyl acetate, butyl acetate, methanol, ethanol and isobutanol) and three binary solvents (N,N-dimethylformamide + acetonitrile, tetrahydrofuran + acetonitrile, methyl acetate + acetonitrile) at different temperatures (273.15–323.15 K) was determined by static equilibrium-high performance liquid chromatography. The experimental results showed that the solubility of MFCA increased with increasing temperature in tested solvents, and the solubility of MFCA in N,N-dimethylformamide was maximal. The Hansen solubility parameter was used to research the dissolution behavior of MFCA. Meanwhile, the solvent effect was discussed by KAT-LSER model in ten mono-solvents, and it was found that the hydrogen bond basicity and nonspecific dipolarity/polarizability were favorable to the dissolution of MFCA. In addition, modified Apelblat model, λh model, CNIBS/R-K model and Jouyban-Acree model were used to analyze the correlation and evaluate the applicability for the experimental solubility data of MFCA. The calculated data showed that modified Apelblat model and CNIBS/R-K model are the best models for mono and binary solvents, respectively. Finally, the dissolution thermodynamic properties of MFCA were studied by van't Hoff analysis, indicating that the dissolution of MFCA was an endothermic and entropic driven process. To sum up, these experimental results could provide pretty reference for purification and crystallization optimization of MFCA, and realize the industrial large-scale production of various drugs such as diuretic.
研究领域:
药理学
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物理化学
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DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Chen Liu,Yutang Kang,Yuanyuan Rao,Shasha Feng,Feng Zhang,Zhaoxiang Zhong,Weihong Xing
In this study, a polyacrylonitrile (PAN) nanofibrous membrane demonstrating both high particulate matter filtration efficiency and formaldehyde visual detection capabilities was obtained by electrospinning. The additive polyvinylpyrrolidone (PVP) was introduced to adjust the structural characteristics and hydrophilicity of the membrane, while methyl yellow (MY) was added as a colorimetric indicator. Sulfamic acid was then loaded on the membrane to obtain the sensing property. The obtained nanofibrous membrane exhibits excellent PM0.3 filtration efficiency (99.88%) with low filtration pressure drop (159.3 Pa). The sensing accuracy for formaldehyde can reach below 50 ppb (World Health Organization, safety concentration <80 ppb), and it can identify formaldehyde at a concentration of 1 ppm within 10 min. Owing to the specific reaction between sulfamic acid and formaldehyde, the membrane exhibited excellent anti-interference performance against other volatile organic compounds. This nanofiber colorimetric sensing membrane holds great potential for application in respiratory protective equipment.
研究领域:
材料科学
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环境健康
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呼吸防护
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纳米技术
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分析化学
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功能材料
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 27
·12934-12940
·
IF 3.925
Hansen Yang,Zhimiao Wang,Hualiang An,Qiusheng Yang,Wei Xue,Fang Li,Yanji Wang
Theobromine is an important pharmaceutical intermediate and food additive. We have developed a green method for theobromine synthesis, namely catalyst-free methylation of 3-methylxanthine with dimethyl carbonate (DMC). The effects of various solvents on the reaction were investigated; H2O was found to be the most appropriate solvent. The bond energies of C–O in DMC and N–H in 3-methylxanthine were calculated at the M06-2x/6-311G(d,p) level, and the role of the solvent was examined theoretically. The use of H2O as the solvent and the effects of DMC hydrolysis on the reaction were investigated. The results show that DMC hydrolysis was, to some extent, beneficial to theobromine synthesis. The reaction conditions were optimized. When the molar ratio of DMC to 3-methylxanthine was 11 : 1 and the 3-methylxanthine dosage was 0.1 g per mL-H2O, reaction at 160 °C for 9 h gave a 3-methylxanthine conversion of 89.9% with 98.2% theobromine selectivity.
研究领域:
药物化学
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绿色化学
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有机合成
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Chang Liu,Yinuo Wu,Huizhe Zhao,Xiangyu Gu,Jinyang Gu,Mengmeng Zhao,Shangci Zuo,Pengchao Wang
7-Methylxanthine (7-MX), a caffeine (1,3,7-trimethylxanthine) derivative, has gained significant attention as a potential drug for myopia treatment. However, the efficient production of this valuable compound poses challenges: Isolation and chemical synthesis of 7-MX are both difficult to realize due to their poor yields and high costs. Therefore, developing efficient biosynthetic pathways has emerged as a promising alternative strategy. This study aimed to establish an efficient, low-cost, and pollutant-free biosynthetic process for producing 7-MX from caffeine, in which the biosynthetic process is achieved by utilization of ndmA, ndmB, and modified ndmD genes. Moreover, the rich caffeine in coffee waste can be used as an ideal substrate for this reaction, which can reduce the production cost and treat the caffeine in coffee waste residue to promote secondary utilization. By optimizing the gene expression, constructing cofactor regeneration system composed of frmA, frmB, and FDH to regenerate NADH to remove the bottleneck, and engineering Escherichia coli for high-density fermentation, we increased the production of 7-MX to an unprecedented 8.37 g/L. This approach represents the most efficient method thus far for producing 7-MX from caffeine and provides insights into synthesizing other valuable methylxanthines.
研究领域:
生物技术
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药学
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代谢工程
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DOI
高影响因子
Communications Biology
·
2024
·Vol. 7
·期 1
·1-11
·
IF 5.2
Qin Danhui,Song Hui,Wang Caiwei,Ma Xiaojie,Fu Yu,Zhao Chunyuan,Zhao Wei,Zhang Lei,Zhang Weifang
Stimulator of interferon genes (STING) is vital in the cytosolic DNA-sensing process and critical for initiating the innate immune response, which has important functions in host defense and contributes to the pathogenesis of inflammatory diseases. Zinc finger CCCH-type antiviral protein 1 (ZC3HAV1) specifically binds the CpG dinucleotides in the viral RNAs of multiple viruses and promotes their degradation. ZAPS (ZC3HAV1 short isoform) is a potent stimulator of retinoid acid-inducible gene I (RIG-I) signaling during the antiviral response. However, how ZC3HAV1 controls STING signaling is unclear. Here, we show that ZC3HAV1 specifically potentiates STING activation by associating with STING to promote its oligomerization and translocation from the endoplasmic reticulum (ER) to the Golgi, which facilitates activation of IRF3 and NF-κB pathway. Accordingly, Zc3hav1 deficiency protects mice against herpes simplex virus-1 (HSV-1) infection- or 5,6-dimethylxanthenone-4-acetic acid (DMXAA)-induced inflammation in a STING-dependent manner. These results indicate that ZC3HAV1 is a key regulator of STING signaling, which suggests its possible use as a therapeutic target for STING-dependent inflammation.
研究领域:
免疫学
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病毒学
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炎症性疾病
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DOI
PubMed
高影响因子
OPHTHALMIC RESEARCH
·
2022
·Vol. 65
·期 6
·678-684
·
IF 3.031
Fan Yuzhuo,Li Jiarui,Huang Lvzhen,Wang Kai,Zhao Mingwei
Introduction: The goal of this study was to investigate the presence of ADORA2A-DRD2 heterodimers in human retinal pigment epithelial (RPE) cells; determine if 7-methylxanthine (7-MX), a nonselective adenosine receptor antagonist which was used to control myopia progression, can influence the behavior of RPE cells through the ADORA2A-DRD2 receptor pathway; and assess the changes in the expression of signaling molecules during cellular signal transduction. Methods: Human RPE cells were cultured in vitro in the presence or absence of 7-MX. Cell proliferation was evaluated with the CCK-8 assay. Apoptosis and necrosis rates were determined by annexin V–FITC/propidium iodide staining and flow cytometry. Immunofluorescence and coimmunoprecipitation were used to examine the protein expression and colocalization of ADORA2A and DRD2 in RPE cells. ADORA2A and DRD2 were knocked down with small interfering RNAs (siRNAs). Changes in the protein expression of ERK1/2 and phospho-ERK1/2 (pERK 1/2), which are signaling molecules downstream of dopamine receptors, were evaluated by Western blot analysis. Results: Immunofluorescence and coimmunoprecipitation showed that ADORA2A and DRD2 were colocalized in RPE cells. The expression of ADORA2A in RPE cells was inhibited by treatment with 50 µmol/L 7-MX for 48 h, and the expression of DRD2, ERK1/2, and pERK1/2 was increased after treatment with 50 µmol/L 7-MX for 48 h. After siRNA-mediated knockdown of DRD2 in RPE cells and further treatment with 50 µmol/L 7-MX for 48 h, the expression of DRD2 was nearly restored to the level observed in the native control. At the experimental concentrations, 7-MX and siRNAs did not affect the proliferation or apoptosis of human RPE cells. Conclusions: ADORA2A and DRD2 heterodimers were present in RPE cells. 7-MX may affect the behaviors of RPE cells through the ADORA2A-DRD2 receptor pathway. 7-MX is an inhibitor of ADORA2A receptors that can prevent inhibition of the DRD2 receptor pathway and increase DRD2 receptor pathway activity. This phenomenon may explain the mechanism of action through which 7-MX can control myopia progression
研究领域:
眼科学
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细胞生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2019
·
IF 2.994
Jin Tan,Rong Li,Zi-Tao Jiang,Shu-Hua Tang,Ying Wang
To circumvent the limitation of conventional high performance liquid chromatography (HPLC) method, synchronous front-face fluorescence spectroscopy was applied for the rapid and non-destructive determination of two major methylxanthines (theobromine and caffeine) and cocoa solid contents in dark chocolate for the first time. Total synchronous fluorescence spectra at wavelength interval (Δλ) between 30–100 nm of 21 brands of commercial dark chocolate bars with labeled cocoa solids of 50–100% were directly obtained at front-face geometry. The contents of the two methylxanthines were determined by HPLC. Partial least squares regression (PLSR) models for quantification were constructed, optimized, and validated by both full ( leave-one-out ) cross-validation and external validation. The coefficients of determination ( R 2 ) values are larger than 0.8, and the relative errors of prediction were ca. 10%. The built models were applied to the detection of four real samples and compared with HPLC method. The combination of front-face fluorescence and PLSR provides a fast and non-invasive procedure for the quantification of theobromine and caffeine in dark chocolate, as an effective alternative for the traditional HPLC method. The further application of the proposed strategy is the rough estimation of cocoa solids in dark chocolate.
研究领域:
食品科学
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分析化学
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光谱学
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产品关联
DOI
高影响因子
FUEL
·
2018
·
IF 4.908
Youan Ji,Yucui Hou,Shuhang Ren,Congfei Yao,Weize Wu
Phenolic compounds, which are widely used in producing synthetic fibers, engineering plastics, and other industrial organic chemicals, can be obtained from oil mixtures before further processing or applications by separation. Based on Lewis/Brønsted acid-base theory, we proposed the separation of phenolic compounds from oil mixtures using some environmentally benign biological reagents (BRs, including 1,3-dimethylxanthine-DMX, isonicotinamide-INA, and L-lysine-LYS). The effects of stirring time, temperature, initial phenol concentration, and BR:phenol mole ratio on phenol removal were studied in detail. LYS, INA and DMX show phenol removal efficiencies of 98.4%, 82.2%, and 66.6% from toluene at BR:phenol mole ratios of 1.6, 1.4, and 0.4, respectively, and those of 99.1%, 91.8%, and 74.6% from n -hexane at BR:phenol mole ratios of 2.20, 1.60, and 0.80, respectively, at room temperature. The BRs were regenerated with an anti-extraction method for four cycles without significant changes in separation efficiency and their properties. In addition, it is found that there is little toluene entrained in the biological reagents, which is as low as 2.3 wt% of separated phenol, indicating high purities of products. These BRs show the advantages of easy biodegradation, no corrosion to the equipment and containers, and no pollution to the environment.
研究领域:
化学工程
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环境科学
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材料科学
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产品关联
DOI
高影响因子
Materials Chemistry Frontiers
·
2019
·Vol. 3
·期 5
·922-930
·
IF 0
Guang Wu,Lingling Zhao,Hongze Liang,Yinghua Yan,Hui Tan
Polymeric hairy microspheres with a Janus hollow structure were prepared via cationic polymerization using SiO2 nanoparticles as interface protection cores and sacrificial templates. Sulfonic acid groups (–SO3H) were introduced onto the surface of the microspheres by sulfonation and facilitated the further loading of titania. The Janus hollow polymeric hairy microspheres (HSPHS) could be applied as efficient adsorbents to remove both hydrophilic dye rhodamine B (RB) and hydrophobic dye dimethyl yellow (DMY) from the surrounding solution, and the results showed that >98% of RB or/and DMY could be removed within several minutes. The maximum adsorption amount on the Janus HSPHS was 3.48 and 0.14 mol g−1 for RB and DMY, respectively. In addition, the HSPHS can also serve as catalyst scaffolds to load anatase titania for the photocatalytic degradation of RB. Janus hollow microspheres coated with anatase titania (HSPHS@TiO2-An) demonstrated a remarkable photocatalytic efficiency and recyclability. High photocatalytic activity was observed for HSPHS@TiO2-An and the catalytic efficiency remained with almost no loss of the photocatalytic activity even after five catalytic cycles. Thus, the Janus hollow polymeric hairy microspheres have promising applications in water treatment as efficient adsorbents and catalyst scaffolds.
研究领域:
材料科学
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环境科学
·
化学工程
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产品关联
DOI
危险品化学品经营许可证（带存储）