Experimental and Therapeutic Medicine
·
2014
·Vol. 7
·期 6
·1738-1744
·
IF 0.941
Zhong‑Lu Ye,Xiao‑Xiao Hou,Re‑Ling Chen,Jie Ding,Guo‑Hua Zheng,Ming‑Zhen Chen,Chuan Tian
The aim of the present study was to investigate the effects of methylthiouracil (MTU) on the proliferation and apoptosis of rat bone marrow stromal cells (BMSCs). Rat BMSCs were isolated, cultured in vitro and treated with various concentrations of MTU. Cell growth curves were determined using the Cell Counting Kit‑8 method and the effect of MTU on BMSCs in a logarithmic growth phase was observed. BMSC apoptosis following MTU treatment was detected by flow cytometry. The experimental results demonstrated that the proliferation‑inhibition effect was gradually enhanced with increasing MTU concentrations and the extension of treatment time. Statistically significant differences were observed between the treatment and the control groups (P<0.05). In addition, the BMSC apoptosis rate gradually increased with increasing drug concentrations and treatment time extension; statistically significant differences were observed between the treatment and the control groups (P<0.05). Therefore, the results of the present study demonstrated that MTU inhibited the proliferation of BMSCs and promoted apoptosis, indicating the cytotoxic effects of MTU on BMSCs.
研究领域:
细胞生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
SPECTROSCOPY LETTERS
·
2025
·
IF 1.6
Ning Jiang,Sha-li Tian,Yao-tang Wang,Shuang-jiao Sun
The hydrothermal method was employed to synthesize water-soluble fluorescent carbon dots (CDs) derived from yam. The presence of Fe3+ significantly quenches the fluorescence of CDs, while the sulfhydryl group (-SH) in methimazole (MML) acts as a reducing agent for Fe3+, leading to fluorescence recovery upon the addition of MML. The experimental results demonstrated a robust linear correlation between the recovery of fluorescence intensity (△F) at 380 nm and the concentration of MML within the range of 0.050 µg/mL to 2.00 µg/mL. The linear equation was △F = 262.06c + 187.93, exhibiting a high correlation coefficient of 0.9993 and a detection limit as low as 0.017 µg/mL. When applied for determining the content of MML in tablet samples, the experimental outcomes yielded satisfactory results.
研究领域:
分析化学
·
药物分析
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DOI
高影响因子
RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2021
·Vol. 57
·期 4
·395-400
·
IF 1.078
Jing Liu,Ma Lu-Teng,Ling Ai-Xia,Lv Mei,Kong Fan-Dong,Wang Jun
Abstract Au/XC-72 nanoparticles prepared via microwave-assisted polyol processes were used as the active materials for methimazole detection. The Au/XC-72 modified electrode shows an excellent catalytic activity toward oxidation of methimazole, the cyclic voltammetry of methimazole displays two pairs of well-defined, quasi-reversible redox peaks. The oxidation of methimazole on Au/XC-72 modified electrode is a diffusion-controlled and a one-electron transfer process. The fixed-potential amperometry technique was used for the determination of methimazole and a linear dependence of anodic current on methimazole concentration was obtained in the range of 19.9–100.91 μM.
研究领域:
分析化学
·
药物分析
·
电化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Chunhe Yu,Dongmiao Qin,Xiaohua Jiang,Xiangfei Zheng,Biyang Deng
In this work, bright yellow fluorescent nitrogen-doped carbon quantum dots (YN-CQDs) are produced by a facile, inexpensive and eco-friendly hydrothermal technique using extract of chlorophyll from lettuce leaf as precursor. The quantum yield (QY) of as-synthesized YN-CQDs was up to 38%, which was higher than the previously reported quantum yield of most CQDs prepared using other biomass. Silver ion (Ag + ) can covalently bond with carboxyl, hydroxyl and amino groups on the surface of YN-CQDs and quench the fluorescence of YN-CQDs through static quenching. Upon addition of methimazole (MZ) to the YN-CQDs/Ag + dispersion system, Ag + releases from the YN-CQDs/Ag + complex due to the high affinity of MZ for Ag + and the quenched fluorescence of the YN-CQDs was recovered. Hence, we developed an off–on sensing method for the detection of MZ using YN-CQDs/Ag + as a fluorescent probe. Under optimal conditions, with addition of MZ, the fluorescence recovery of YN-CQDs/Ag + system happens with a good linearity in the range of 0.003 ~ 60 μmol/L and a detection limit of 0.8 nmol/L. The YN-CQDs/Ag + system exhibited good MZ determination performance. The established off–on fluorescence sensing platform was successfully employed for detecting MZ in mouse plasma.
研究领域:
分析化学
·
生物医学
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Fei Wei,Xueyi Wu,Hongyuan Wang,Yongpu Zhang,Lei Xie
Methimazole (MMI) is an emerging endocrine disrupting chemical (EDC) due to its increasing use in the treatment of thyrotoxicosis (hyperthyroidism), but its potential impact on amphibian development remains largely unexplored. In the present study, the effects of 8 mg/L MMI and 1 μg/L thyroxine (T4) exposure on skeletal ossification and muscle development in Bufo gargarizans tadpoles were comprehensively investigated by double skeletal staining, histological analysis and RNA sequencing. Our results indicated that MMI treatment down-regulated the expression levels of ossification-related genes (e.g., BMPs , MMPs , and Wnt9a ) in cartilage, thereby delaying chondrocyte apoptosis and inhibiting hindlimb ossification. Muscle sarcomere was elongated in both the MMI and T4 treatment groups, which may lead to muscle weakness and consequently affect land motion. Additionally, we evaluated the expression levels of fast muscle-related genes ( TNNI2 and TNNT3 ) and slow muscle-related genes ( TNNI1 and TNNT1 ), revealing an opposite trend in the transition from fast to slow muscle after T4 and MMI exposures. In conclusion, these findings fill the data gap regarding MMI contamination in aquatic environments by revealing the negative effects of MMI on amphibian bone and muscle development. Future studies should address the toxicity of EDCs to wildlife and inform aquatic ecosystem conservation strategies.
研究领域:
生态毒理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Yuting Long,Hong Li,Wenbo Wang,Xinxin Yang,Zirui Liu
Ultrasensitive detection of highly toxic Cr(VI) is of paramount importance as trace amount of Cr(VI) will result in inevitable deterioration to human health and the environment. In this work, a novel methimazole-functionalized SERS optical fiber probe was used for the first time for Cr(VI) determination with ultrahigh sensitivity. A special functionalization process of methimazole was designed after successful fabrication of silver coated fiber probe via a SnCl 2 -sensitized solvothermal method. Dual functional methimazole guaranteed ultrahigh sensitivity and good stability of the SERS fiber probe for Cr(VI) detection. On one hand, methimazole functions as a Raman reporter which exhibits SERS peak intensity decline in the presence of Cr(VI). On the other hand, methimazole acts as an oxidation inhibitor via coordinating with silver atoms through the lone pair of nitrogen and sulfur atoms. The optimized SERS fiber probe not only presents good stability and excellent reproducibility, but also exhibits ultrahigh sensitivity (10 −11 M) which exceeds SERS or other techniques for Cr(VI) determination reported so far.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Xiaolei Zhao,Yu He,Yanan Wang,Shuo Wang,Junping Wang
In this study, a novel detection method of methimazole was proposed based on the hollow molecularly imprinted quartz crystal microbalance (QCM) sensor, in which the hollow imprinted polymers (H-MIPs) were firstly prepared through the surface imprinted techniques, using hollow silica spheres as matrix supporting material and methimazole as template molecule. The characterizations of H-MIPs were carefully studied. Compared with traditional MIPs, H-MIPs exhibited faster mass transfer rate and higher adsorption capacity. After coating onto the surface of Au chip, the H-MIPs QCM sensor was fabricated. Based on the frequency shift, good linear behavior in the range of 5–70 μg L –1 , limit of detection of 3 μg L –1 , and good recoveries of 88.32%–107.96% in the spiked pork, beef and milk were obtained. The analysis process could complete within 8 min. The developed sensor provided an effective, fast and accurate method for the methimazole detection in food samples.
研究领域:
分析化学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
Processes
·
2024
·Vol. 12
·期 3
·626
·
IF 2.8
Yan Liu,Tian Zhao,Shuzhen Li,Yichuan Cao,Guozhen Fang
Rapid detection and sensitive analysis of MMZ is of great importance for food safety. Herein, a fluorescent molecularly imprinted sensor based on upconversion nanoparticles (UCNPs) grafted onto covalent organic frameworks (COFs) was designed for the detection of MMZ. COFs with a high specific surface area and excellent affinity serve as substrates for grafting of UCNPs, which can inhibit the aggregation burst of UCNPs and improve the mass transfer rate of the sensor. Through a series of characterizations, it was found that the proposed UCNP-grafted COFs@MIP-based sensor had good optical stability, high adsorption efficiency, strong anti-interference ability, and high sensitivity owing to the integration of the advantages of UCNPs, COFs and MIPs. Under the optimal conditions, a good linear relationship was presented between the fluorescence intensity of UCNP-grafted COFs@MIPs and the methimazole concentration in the range of 0.05–3 mg L−1, and the detection limit was 3 μg L−1. The as-prepared UCNP-grafted COFs@MIPs were successfully applied for the detection of MMZ in actual samples, and the results were relevant with those determined by high-performance liquid chromatography. The sensor has good sensitivity, reusability, and high selectivity, which are highly valuable in the rapid analysis and detection of food safety.
研究领域:
食品安全
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY LETTERS
·
2023
·Vol. 45
·期 7
·873-883
·
IF 2.7
Zhang Zhenxing,Meng Min,Wu Qiong,Kim Jeong-Han,Zhu Yongzhe
Quinalphos is a long-term, wide-spectrum organophosphate insecticide with residual problems in the natural environment. Cunninghamella elegans ( C. elegans ) is a member of Mucoromycotina. Since the degradation products of its exogenous compounds are similar to those of mammals, it is often used to simulate the metabolism pathways of mammals. In this study, the detailed metabolic pathways of quinalphos were investigated with C. elegans . Quinalphos was degraded by 92% in 7 days, while ten metabolites were produced. The metabolites were analyzed and identified by GC–MS. To determine the responsible enzymes in quinalphos metabolism, piperonyl butoxide (PB) and methimazole included in the culture flasks, and the kinetic responses of quinalphos and its metabolites by C. elegans were measured. Results indirectly demonstrated that cytochrome P450 monooxygenases were involved in the metabolism of quinalphos, but that methimazole inhibited the metabolism less efficiently. Comprehensive metabolic pathways can be deduced from the detailed analysis of metabolite profiles in control and inhibitor assays.
研究领域:
环境科学
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Shanshan Wu,Xinyu Li,Bijun Wang,Jingshuo Jiang,Di Tao,Zhenhong Xia,Hang Gao,Ningning Wang
The development of convenient and highly sensitive methodologies for evaluating drug levels in serum is crucial for clinical diagnosis. As the most prescribed drug against hyperthyroidism, methimazole (MMZ) lacks the simple and rapid quantitative method. Herein, an electrochemiluminescent (ECL) sensor is proposed for MMZ detection using an aggregation-induced delayed ECL (AIDECL) active organic nanodots (ODs) as a probe. Due to the full utilization of excited species, these ODs exhibit high ECL efficiency. In the presence of MMZ, such sensor exhibits decreased ECL signal of ODs since MMZ can consume co-reactants’ radical cations that are vital mediates for ECL emission of ODs. Consequently, the developed sensor demonstrates excellent performance for the analysis of MMZ with a low detection limit of 7 × 10 −7 μM, good specificity, and excellent stability. Significantly, this sensor has been successfully applied to serum sample with good recovery rate. This work provides a valuable strategy for accurate detection of MMZ, and demonstrates its application potential in clinical research.
研究领域:
分析化学
·
临床诊断
·
药物检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 22
·13446-13457
·
IF 3.361
Chu Hongtao,Chen Jiaqi,Yao Dong,Yu Miao,Lin Qing,Zhang Jingru
Dendritic fiber-type silica (KCC-1) has attracted the attention of researchers because of its unique three-dimensional radial structure and high specific surface area. Its highly modified surface allows it to be used in catalysis, adsorption, biomedicine, and other fields. Nano-precious metals (NPs) have several excellent chemical properties, but their stability limits their applications. Dendritic fibrous silica (Ag NPs/KCC-1) loaded with silver nanoparticles was prepared via the microemulsion method using Ag NPs/KCC-1 as the carrier, methimazole as the template molecule, and a surface imprinting method to prepare sulfhydryl imidazole molecularly imprinted polymer. By characterizing the polymer, it is determined that the polymer has a regular morphology and large specific surface area. The obtained experimental results show that the polymer has a high adsorption capacity (10.35 mg g−1) and good selectivity. It is used as a solid-phase extraction filler and, when combined with high-performance liquid chromatography, to detect methimazole in chicken tissue. The recovery rate reaches 87.5–94.4%.
研究领域:
材料科学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
·
2023
·Vol. 116
·期 12
·1385-1393
·
IF 2.6
Meng Min,Zhai Zhaochi,Zhang Zhenxing,Kim Jeonghan,Zhu Yongzhe
Tebuconazole is the most widely used fungicide in agriculture. Due to its long half-life, tebuconazole residues can be found in the environment media such as in soil and water bodies. Here, the metabolic pathway of tebuconazole was studied in Cunninghamella elegans ( C. elegans ). Approximately 98% of tebuconazole was degraded within 7 days, accompanied by the accumulation of five metabolites. The structures of the metabolites were completely or tentatively identified by gas chromatography-mass spectrometry (GC–MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). To identify representative oxidative enzymes that may be involved in the metabolic process, treatment with piperonyl butoxide (PB) and methimazole (MZ) was performed. PB had a strong inhibitory effect on the metabolic reactions, while MZ had a weak inhibitory effect. The results suggest that cytochrome P450 (CYP) and flavin-dependent monooxygenase are involved in the metabolism of tebuconazole. Based on the results, we propose a metabolic pathway for the fungal metabolism of tebuconazole. Data are of interest to gain insight into the toxicological effects of tebuconazole and for tebuconazole bioremediation.
研究领域:
环境科学
·
微生物学
·
毒理学
·
生物修复
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）