ANALYST
·
2013
·Vol. 138
·期 7
·2051-2056
·
IF 3.969
Qian Han,Yonghua Wang,Yihan Huang,Liju Guo,Yingzi Fu
A chiral interface has been designed for specific recognition of carboxylic acids using multilayer architectures of β-cyclodextrin (β-CD) and methylene blue/reduce-graphene (MB@rGO) on glassy carbon electrodes. The advantages of β-CD as a chiral selector and MB@rGO composite as an electrochemical indicator were perfectly presented in this novel interface. It displayed good redox signal for sensing chiral target with high sensitivity and conductivity. Enormous signal differences were obtained after adsorption of target L isomer, due to strong blocking of the electron transfer process of methylene blue. Meanwhile mandelic acid was found to be the best chiral guest and obtained more effective chiral recognition.
研究领域:
分析化学
·
电化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Xiaoyun Lin,Yongnian Ni,Serge Kokot
An electrochemical biosensor capable of indirect detection of DNA damage induced by any one of the three endocrine-disrupting compounds (EDCs) – bisphenol A (BPA), 4-nonylphenol (NP) and 4- t -octylphenol (OP), has been researched and developed. The methylene blue (MB) dye was used as the redox indicator. The glassy carbon electrode (GCE) was modified by the assembled dsDNA/graphene oxide-chitosan/gold nano-particles to produce a dsDNA/GO-CS/AuNPs/GCE sensor. It was characterized with the use of electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and scanning electron microscopy (SEM) techniques. The loading/release of the MB dye by the dsDNA/GO-CS/AuNPs film was investigated, and the results showed that the process was reversible. Based on this, the sensor was used to measure the difference between the loading capabilities of intact and damaged dsDNA in the films. The sensor was then successfully applied to detect DNA damage electrochemically. The differential pulse voltammetry (DPV) peak current ratio for MB, observed before and after DNA damage, increased linearly in the presence the BPA, NP or OP compounds; the treatment range was 10–60 min, and the respective damage rates were 0.0069, 0.0044 and 0.0031 min −1 , respectively. These results were confirmed by the binding constants: 2.09 × 10 6 M −1 (BPA-DNA), 1.28 × 10 6 M −1 (NP-DNA) and 9.33 × 10 5 M −1 (OP-DNA), all of which were obtained with the use of differential pulse stripping voltammetry (DPSV).
研究领域:
生物化学
·
环境科学
·
毒理学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yong Chang,Fengli Gao,Tong Wu,Qiuzhi Pan,Lin Liu,Qijun Song
Protein kinases play a vital regulatory role in various biological processes. In this work, we developed an electrochemical biosensor for the detection of protein kinases using methylene blue (MB)-functionalized Zr-based metal-organic frameworks (MOFs, UiO-66-NH 2 ) as the signal labels. The results of UV-Vis spectroscopy, X-ray diffraction and N 2 adsorption–desorption experiments confirmed the successful encapsulation of MB into UiO-66-NH 2 . The phosphate groups in phosphorylated peptides were recognized by UiO-66-NH 2 via the robust coordination with Zr-O clusters on the surface of MOFs. The encapsulated MB molecules in UiO-66-NH 2 produced a strong electrochemical signal for highly sensitive detection of protein kinases, which was recorded by square wave voltammetry (SWV). With protein kinase A (PKA) as a model target, the biosensor achieved a detection limit of 0.02 U/mL and a linear range from 0.05 to 20 U/mL. The proposed biosensor was utilized to screen potential inhibitors and determine PKA in serum samples with satisfactory results. This work provided an effective strategy for electrochemical assays of phosphorylation-related biological events in clinical diagnosis.
研究领域:
生物化学
·
临床诊断
·
分析化学
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DOI
高影响因子
Optical Materials Express
·
2017
·Vol. 7
·期 2
·409-414
·
IF 2.591
Junliang Lv,Gongqing Wu,Yang He,Lijuan Zhang,Yunhong Yi
Plasmonic nanoparticles hold great potential in photodynamic therapy (PDT). Herein, a nanoplatform of methylene blue (MB) loaded gold nanobipyramids@SiO2 (GBPs@SiO2-MB) was designed to optimize the generation rate of singlet oxygen (1O2), which was based on the plasmonic effect. The surface plasmon resonance (SPR) of GBPs was finely overlapped with the excitation absorption of MB, a wildly applied organic PDT drug prone to enzymatic degradation. This mesoporous silica coating nanoplatform protects MB molecules against degradation, and this overlap greatly enhances the 1O2 yield of MB by the SPR electron from the GBPs. The GBPs@SiO2-MB nanoparticles exhibit a synergistic effect of PDT and photothermal therapies (PTT) of cancer cells under laser irradiation. This study provides a alternative strategy to improve the classic MB treatment for phototherapy application.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
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DOI
高影响因子
CHIRALITY
·
2021
·Vol. 33
·期 7
·385-396
·
IF 2.437
Qiuna Lu,Lei Chen,Qi Meng,Yan Jiang,Licheng Xie
A composite chiral interface (BSA-MB-MWCNTs) was prepared from bovine serum albumin (BSA), methylene blue (MB), and multi-walled carbon nanotubes (MWCNTs) for chiral recognition of amine enantiomers (1 S , 2 S )- N , N ′-dimethyl-1,2-cyclohexanediamine and (1 R , 2 R )- N , N ′-dimethyl-1,2-cyclohexanediamine. The BSA-based composite was characterized by field emission scanning electron microscopy (FESEM) and ultraviolet–visible spectroscopy (UV–Vis). The electrochemical responses towards the two enantiomers were analyzed via cyclic voltammetry (CV), electrochemical AC impedance method (EIS), and differential-pulse voltammetry (DPV). The experimental results showed that the combination of MWCNTs and BSA could effectively improve the overall identification efficiency, and the peak current displayed by the S -enantiomer is larger, indicating that the prepared chiral surface has stronger interaction with the R -enantiomer. Under optimized condition, the current value of the oxidation peak of the chiral modified electrode showed a good linear relationship towards the amine concentration in the range of 5.0 × 10 −3 to 5.0 × 10 −5 mmol·L −1 . The proposed electrochemical chiral interface is easy to handle and provides a promising electrochemical sensing platform that can be used to identify chiral amine enantiomers.
研究领域:
生物化学
·
电化学传感
·
材料科学
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DOI
高影响因子
Biosensors-Basel
·
2024
·Vol. 14
·期 10
·457
·
IF 4.9
Zhuo Liu,Fengyu Liu,Chaofan Wang,Hongjuan Li,Yongqian Xu,Shiguo Sun
Herein, we report a ratio-based electrochemical biosensor for the detection of interleukin-6 (IL-6). We electropolymerized methylene blue (MB) on the surface of screen-printed carbon electrodes; introduced an internal reference signal probe; modified the carboxylate multi-walled carbon nanotubes on the electrode surface to increase the electrochemically active area; and finally linked the amino-modified IL-6 aptamer to the electrode surface through the Schiff base reaction, with bovine serum albumin (BSA) added to mask non-specific adsorption. After adding IL-6 to the samples, the signal of I MB remained almost unchanged, while the signal of I[Fe(CN) 6 ] 3−/4− decreased with increasing IL-6 concentration. Thus, a novel ratiometric electrochemical sensor with a linear range of 0.001~1000.0 ng/mL and a low detection limit of 0.54 pg/mL was successfully developed. The sensor had high repeatability, stability, sensitivity, and practicability. It provides a new method for constructing proportional electrochemical sensors and detecting IL-6.
研究领域:
生物传感技术
·
免疫学
·
纳米材料应用
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jianwen Wan,Yiyi Liang,Qiong Hu,Zhiwen Liang,Wenxing Feng,Yiyan Tian,Shiqi Li,Zhuojun Ye,Mingru Hong,Dongxue Han,Li Niu
The rapid quantification of therapeutic monoclonal antibodies (mAbs) is of great significance to their pharmacokinetics/pharmacodynamics (PK/PD) research and the personalized medication for disease treatment. Taking advantage of the direct decoration of tens of redox tags to the target of interest, we illustrate herein an amplification-free ratiometric electrochemical aptasensor for the point-of-care (POC) detection of trace amounts of therapeutic mAbs. The POC detection of therapeutic mAbs involved the use of the methylene blue (MB)-conjugated aptamer as the affinity element and the decoration of therapeutic mAbs with ferrocene (Fc) tags via the boronate crosslinking, in which the MB-derived peak current was used as the reference signal, and the peak current of the Fc tag was used as the output signal. As each therapeutic mAb carries tens of diol sites for the site-specific decoration of the Fc output tags, the boronate crosslinking enabled the amplification-free detection, which is cost-effective and quite simple in operation. In the presence of bevacizumab (BevMab) as the target, the resulting ratiometric signal (i.e., the IFc/IMB value) exhibited a good linear response over the range of 0.025–2.5 μg/mL, and the limit of detection (LOD) of the electrochemical aptasensor was 6.5 ng/mL. Results indicated that the aptamer-based affinity recognition endowed the detection of therapeutic mAbs with high selectivity, while the ratiometric readout exhibited satisfactory reproducibility and robustness. Moreover, the ratiometric electrochemical aptasensor is applicable to the detection of therapeutic mAbs in serum samples. Taking together, the amplification-free ratiometric electrochemical aptasensor holds great promise in the POC detection of therapeutic mAbs.
研究领域:
生物医学工程
·
分析化学
·
药物分析
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）