JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2010
·Vol. 52
·期 4
·615-619
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IF 2.453
Shenguang Ge,Mei Yan,Xiaoliang Cheng,Congcong Zhang,Jinghua Yu,Peini Zhao,Weiqiang Gao
A simple and convenient on-line molecular imprinted solid-phase extraction flow-injection fluorescence sensor was developed in this study. Florfenicol (FF)-imprinted polymer was prepared by self-assembly with acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The binding characteristics of the imprinted polymer to florfenicol were evaluated by equilibrium binding experiments, and the morphology of the imprinted polymer was studied by scanning electron microscope (SEM). Fluorescence intensity of 3-p-nitrylphenyl-5-(2′-sulfonophenylazo) rhodanine (4NRASP) and bovine serum albumin (BSA) was inhibited by FF. Under optimum conditions the intensity of fluorescence shows a linear relationship with the concentration of FF over the range of 1.2 × 10 −6 to 2.6 × 10 −5 g mL −1 with a regression equation of Δ I = 23.54 + 17.86 c ( c 10 −6 g mL −1 ) ( r = 0.9965, n = 6). The low detection limit of FF was found to be 3.4 × 10 −7 g mL −1 according to 11 parallel determinations for the blank solution. The relative standard deviation for the determination of 2.0 × 10 −6 g mL −1 FF solution was 3.5% ( n = 11). This proposed sensor could be satisfactorily applied to the determination of FF in liver and meat samples.
研究领域:
分析化学
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药物残留检测
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食品安全
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分子识别
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环境监测
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兽药代谢
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Food Analytical Methods
·
2026
·Vol. 19
·期 10
·303
·
IF 3
Tu Xiaodong,Yao Mingcai,Dong Liang
A chloramphenicol (CAP) electrochemical sensor based on zinc tungstate and maghemite nanocomposites (ZnWO 4 /γ-Fe 2 O 3 ) is proposed. First, ZnWO 4 and γ-Fe 2 O 3 nanomaterials were synthesized using a hydrothermal synthesis method, then compositely modified onto glassy carbon electrodes to construct electrochemical sensors for measuring CAP. Under optimal conditions, the ZnWO 4 /γ-Fe 2 O 3 -modified electrode exhibits a stronger reduction peak current for CAP detection than the single-component electrode and demonstrates better electrocatalytic activity. The results demonstrate that ZnWO 4 /γ-Fe 2 O 3 /GCE exhibits a linear detection range of 8–1000 μM and a detection limit of 1.04 μM, demonstrating good selectivity in the presence of different interferers, as well as outstanding stability and reproducibility. ZnWO 4 /γ-Fe 2 O 3 /GCE was used to detect of CAP in milk samples, yielding satisfactory results.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Zhipeng Liu,Beizhao Chen,Caihong Liu,Youmei Xu,Weiwen Chen,Chaoqun Zhu,Yufei Shu,Shidong Zheng,Fan Wang,Yunjiao Han,Mengxia Wang,Yihan Xue,Zhongying Wang
Chlorinated antibiotics are persistent halogenated pollutants whose detoxification requires deep dechlorination rather than simple parent-compound removal. Here, we report a structurally engineered, earth-abundant copper electrode (Tre-Cu) that enables rapid and deep electrochemical hydrodechlorination of chlorinated antibiotics. Prepared through a facile sequence of oxidation, in situ complex dissolution, and chloride-assisted electroreduction, Tre-Cu undergoes spontaneous surface reconstruction during operation, evolving into a spindle-shaped, sharp-tipped architecture. This electrode achieved nearly complete florfenicol removal within 20 min and full stoichiometric chloride release within 30 min under ambient air, while maintaining stable performance over 10 cycles and 36 h long-term continuous operation, as well as robust tolerance in environmental water matrices. Mechanistic studies show that dechlorination on Tre-Cu is dominated by direct electron transfer rather than atomic hydrogen-mediated reduction. Time-resolved SEM and XPS reveal that transient CuCl-like surface species drive the formation of the activated tip-rich morphology. Finite element simulations, electrochemical analysis, and DFT calculations further demonstrate that the reconstructed surface strengthens the local electric field, enriches interfacial K + , enhances pollutant adsorption, and lowers the barriers for sequential C-Cl bond cleavage. This work offers a simple strategy to couple surface reconstruction with interfacial microenvironment engineering for deep electrochemical detoxification.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 8
·518
·
IF 5.4
Gu Tiance,Shi Haiyan,Liu Dongying,Wang Menghan,Wu Qingqing,Du Shuting,Shi Xueran,Wang Jing,Han Qian
A cerium ion-modified hemin polymer (Ce-hemin) was synthesized via a facile route, exhibiting photo-responsive oxidase-like activity and excellent stability. The Ce doping effectively prevented hemin aggregation, facilitated electron transfer, enhanced photocatalytic activity, and created a high adsorption capacity for tetracyclines (TCs). Taking advantage of the photo-sensitivity of TCs, a “turn-on” colorimetric sensing platform based on Ce-hemin nanozymes and smartphone-readout was constructed for visual analysis of TCs. This sensor offers a wide detection range and a low limit of detection (0.11 μM). The mechanism of the “turn-on” colorimetric performance was systematically discussed, which involves the abundant generation of superoxide radical anion (·O 2 − ) species through photoexcitation of the surface complex between TCs and Ce-hemin. Furthermore, machine learning was employed for the accurate classification and identification of two representative TCs and chloramphenicol. Thus, this work provides an environmentally friendly strategy for the detection of TCs without H 2 O 2 , and also broadens the application of photo-responsive nanozymes in colorimetric sensors. Graphical
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BIOELECTROCHEMISTRY
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2026
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IF 4.9
Hua Ma,Xia Gu,Hao Yang,Qiuyan Lan,Dandan Wang,Yu Huang,Yanru Fan
Misuse of kanamycin in animal husbandry has led to serious food safety issues. In this study, a label-free electrochemical aptasensor based on the base mismatch and strand displacement was developed for detection of kanamycin residue in agricultural products. A novel ferrocene-naphthalimide derivative was employed as the electroactive indicator. The effects of complementary DNA (cDNA) probe length, position and number of mismatched bases in aptamer-cDNA duplex on the performance of displacement-based aptasensor were systematically investigated. The experimental results were in well accordance with the molecular simulation results. Under optimal conditions, the aptasensor exhibited good reproducibility, stability, selectivity, and a wide linear range of 1.0 × 10 −10 –1.0 × 10 −4 g/mL, with a LOD of 1.1 × 10 −11 g/mL (S/N = 3). The proposed aptasensor was used to detect kanamycin residues in milk and chicken liver. Satisfactory recoveries and relative standard deviations indicated that the aptasensor was competent for detection of kanamycin residues in agricultural products.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 4
·280
·
IF 5.4
Deng Gaoqiong,Lu Huanhuan,Han Yulong,Wang Shuo,Chen Hengye,Long Wanjun,She Yuanbin
Innovative cloud algorithms-assisted portable Zn-based fluorescent sensor arrays (ZnMOF-74 and Mn:ZnS QDs) are proposed for rapid, sensitive and on-site detection of four antibiotics, enrofloxacin (ENX), chlortetracycline (CTC), oxytetracycline (OTC) and tetracycline (TC). This sensor arrays for the first time utilize the Lewis acid-base regulation of ZnMOF-74 and the crystal field effect of Mn:ZnS QDs to generate characteristic fluorescent “fingerprints” for the four antibiotics. Through the optimization of various chemometric algorithms (PCA-LDA, PLS-DA, KNN and RF), the recognition accuracy of four antibiotics in the complex matrix reached 98%. Moreover, a homemade smartphone APP based on cloud machine learning algorithms was developed, enabling rapid and accurate multiplexed detection with LODs of 8.9 nmol/L, 4.1 nmol/L, 5.7 nmol/L, and 6.7 nmol/L for ENX, CTC, OTC, and TC, respectively. The corresponding limits of quantification (LOQs) were 26.7 nmol/L, 12.3 nmol/L, 17.1 nmol/L, and 20.1 nmol/L, respectively. The proposed method was successfully applied to the detection of antibiotics in milk, honey, and beef samples, with satisfactory recoveries ranging from 97.33% to 107.40% and relative standard deviations (RSDs) below 5.63%, indicating good accuracy and reliability for real sample analysis. This work demonstrates the immense potential for universal, high-throughput, and accurate multi-target detection, providing transformative solutions in food safety.
Section
Graphical abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Xie Zhoujian,Wu Jiawen,Huang Kejing,Tan Xuecai
Growing needs for monitoring kanamycin (KAN) residues in food require ultrasensitive, field-deployable determination platforms. Herein, we present a universal photoelectrochemical (PEC) aptasensing strategy that synergizes ZIF-8/Bi 2 S 3 heterostructure-engineered photoelectrodes with strand displacement–triggered biocatalytic signal amplification. The urchin-like ZIF-8/Bi 2 S 3 heterojunction, synthesized through in situ MOF growth on semiconductor nanoarchitectures, demonstrates exceptional photocurrent generation via facilitated carrier separation achieved by directional electron transfer from Bi 2 S 3 to ZIF-8 and hole scavenging by ascorbic acid. Capitalizing on aptamer-target recognition in homogeneous phase, KAN concentration is transduced into messenger DNA (S2) through displacement dynamics, overcoming steric limitations of conventional solid-phase biosensing. The liberated S2 hybridizes with electrode-immobilized S1 to assemble MnPP-loaded dsDNA, where the mimic enzyme catalyzes H 2 O 2 -driven precipitation of benzo-4-chlorohexadienone (4-CD). This biocatalytic insulation layer induces quantifiable photocurrent attenuation proportional to KAN levels (10–1.0 × 10 6 nmol/L), achieving a 0.1 nM detection limit superior to ELISA and HPLC. The platform demonstrates 95.7–105.4% recovery in milk matrices and cross-reactivity below 5.8% against structural analogs, validating its specificity. By integrating MOF-semiconductor interfacial engineering with homogeneous-to-heterogeneous signal translation, this work establishes a paradigm for antibiotic monitoring that bridges laboratory innovation with on-site applicability. Graphical abstract
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JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 11.3
Qiaonan Wang,Mingyao Yu,Han Dong,Jingjing Duan,Yanxia Ding,Jinxiao Wang,Yujie Guo,Yaqian Huang,Tao Zhang,Xiaoxing Dong,Fengzhi Piao,Zhixin Guo
Antibiotic pollution in the environment causes serious threats to crop growth and human health. However, effective strategies for alleviating antibiotic stress based on endogenous signaling response to antibiotics in plants remain unclear. Here, we found that the toxic effects of ciprofloxacin (CIP) and tetracycline (TC) at 1 mg L -1 on cucumber seedlings were stronger than lincomycin hydrochloride, sulfacetamide, thiamphenicol and tylosin. Importantly, both CIP and TC induced abscisic acid (ABA) accumulation and the transcripts of ABA-related genes. In turn, spraying exogenous ABA alleviated antibiotics-induced growth and photosynthesis inhibition and oxidative stress dose-dependently, with the most effective concentration of 20 µM. Moreover, ABA inhibited the activities of V-ATPase and V-PPase associated with antibiotics uptake. Subsequently, ABA effectively decreased the root and shoot accumulation of CIP and TC by 41.3% and 58.0%, 62.8% and 85.4%, respectively, compared with CIP and TC stress. Finally, CIP and TC promoted the accumulation of antibiotic resistance genes (ARGs), while ABA significantly declined the relative abundance of target ARGs (e.g., qnrD , tetG-02 and teR-02 ), mobile genetic elements (MGEs) (e.g., cIntI-1 ( class1 ) and intI1 ), and potential host bacteria (e.g., Pseudomonas , Bacillus and Nocardioide s) at the genus level. Network analysis and structural equation models displayed that bacterial communities and MGEs were the vital factors influencing the abundances of ARGs. Overall, these findings demonstrated that ABA confered antibiotic stress tolerance in cucumber by alleviating antibiotic phytotoxicity and decreasing the levels of antibiotics and ARGs, providing a novel and effective method for mitigating the antibiotics contamination in the soil-plant system.
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JOURNAL OF FLUORESCENCE
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2026
·
IF 3.1
Zheng Xinxiang,Xu Hui,Zhang Runke,Zhao Tengzhi,Wang Zhaofeng,Zhao Xingfei,Wang Hua
In this study, a dual-ratiometric optical probe utilizing europium-doped carbon dots (Eu-CDs) was developed for simultaneous fluorescent and colorimetric detection of tetracycline (TC). The Eu-CDs complex was synthesized through a hydrothermal approach. Upon coordination with Eriochrome Black T (EBT), the Eu-CDs complex exhibits a distinct magenta coloration. Introduction of TC triggers a ligand displacement reaction, resulting in color transition from magenta to blue, resulting in ratiometric colorimetric visual detection. Meanwhile, the TC-bound EBT-CDs@Eu complex emits ratiometric fluorescence change. A ratiometric fluorescence detection method was established for TC based on the absorbance-energy transfer-emission (AETE) mechanism, culminating in the successful design of a dual-ratiometric optical probe. The colorimetric assay demonstrated a detection limit (LOD) of 20.5 nM for TC. Furthermore, the practical applicability of this sensor was validated through successful TC detection in aquaculture samples, highlighting its potential for real-world monitoring applications.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
·
IF 4.8
Xiaojing Mao,Huachang Li,Jiemin Liu,Jijun Liu,Pengcheng Han,Haijiao Xie
Fe 3+ and nitrofurazone (NFZ) can raise a risk for environment and human health due to accumulation in water. Hence, a post-synthetic modification strategy was employed to develop a visualization sensor for detecting Fe 3+ and NFZ. In this study, we selected a Zinc-based metal organic framework in combination with fluorescein to develop a fluorescent dyes-encapsulated sensor (Flu@Zn-MOF). The sensing experimental indicated that the Flu@Zn-MOF demonstrated excellent recognition performance for Fe 3+ and NFZ. The detection limits (LODs) of Fe 3+ and NFZ are 1.27 and 0.89 μM, respectively. Furthermore, the color of Flu@Zn-MOF turn from yellow-green to blue in the presence of NFZ. The proposed sensor can accurately identify Fe 3+ and three nitrofuran analogs (NFZ, NFT and FTD) by principal component analysis (PCA). In addition, satisfactory recovery rates and low standard deviation for detection of Fe 3+ and NFZ in real water samples confirms the practicability of Flu@Zn-MOF. Then, a sensing system was created that leverages smartphone technology for the quantitative and real-time monitoring of NFZ. Therefore, this work presents a new strategy for designing a novel sensing method for on-site recognition of nitrofurans pollutants in water.
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TALANTA
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2023
·
IF 6.1
Xuan Ma,Yue Wang,Wenting Wang,Jake Heinlein,Lisa D. Pfefferle,Xuemeng Tian
In this study, a facile method has been developed to synthesize a novel type of porous magnetic molecularly imprinted polymers (Fe 3 O 4 -MER-MMIPs) for the selective adsorption and removal of meropenem. The Fe 3 O 4 -MER-MMIPs, with abundant functional groups and sufficient magnetism for easy separation, are prepared in aqueous solutions. The porous carriers reduce the overall mass of the MMIPs, greatly improving their adsorption capacity per unit mass and optimizing the overall value of the adsorbents. The green preparation conditions, adsorption performance, and physical and chemical properties of Fe 3 O 4 -MER-MMIPs have been carefully studied. The developed submicron materials exhibit a homogeneous morphology, satisfactory superparamagnetism (60 emu g −1 ), large adsorption capacity (11.49 mg g −1 ), quick adsorption kinetics (40 min), and good practical implementation in human serum and environmental water. Finally, the protocol developed in this work delivers a green and feasible method for synthesizing highly efficient adsorbents for the specific adsorption and removal of other antibiotics as well.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
·
IF 4.831
Yuanjie Teng,Zhenni Wang,Shaohua Zuo,Xin Li,Yinxin Chen
Universal and fast antibiotic residues detection technology is imperative for the control of food safety in aquatic products. However, accurate surface-enhanced Raman scattering (SERS) quantitative detection of complicated samples is still a challenge. A recognition method powered by deep learning and took advantage of the unique fingerprint information merits of SERS was proposed. Herein, the spectra were collected by Ag nanofilm SERS substrate prepared by self-assembly of Ag nanoparticles on water/oil interface. A SERS-based database of commonly used antibiotics in aquatic products was set up, which is suitable for employed as input data for learning and training. The results show that the five types of antibiotics are successfully distinguished through principal component analysis (PCA) and each antibiotic in every type was successfully distinguished. Furthermore, one-dimensional convolutional neural networks (1-D CNN) was used to distinguish the antibiotics, and the results show that all the test samples were correctly predicted by 1-D CNN model. The results of this research suggest the great potential of the combination of SERS spectra with deep learning as a method for rapid and highly accurate identification of antibiotic residues in aquatic products.
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