Analytical Methods
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2016
·Vol. 8
·期 6
·1307-1312
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IF 1.915
Haining Wang,Boguang Pan,Taotao Feng,Zhao Sun,Jianming Dan,Xiuwen Qiao,Yu Qi,Chenglin Hong
A novel biomolecular immobilization strategy based on a Cu2O rhombic dodecahedra–polydopamine–gold nanoparticle nanocomposite was used for the detection of alpha-fetoprotein (AFP). Cu2O rhombic dodecahedra, a new kind of Cu2O nanocrystal with excellent catalytic properties, were coated with bio-functional polydopamine (PDA) using a simple self-polymerization in mild basic solution. Then, highly loaded and uniformly dispersed gold nanoparticles (Au NPs) were deposited on Cu2O–PDA through in situ deposition. Under optimal conditions, a linear relationship between the CV peak current and the logarithm of the AFP concentration was obtained from 0.001 to 100 ng mL−1 with a detection limit of 0.6 pg mL−1 at a signal-to-noise ratio of 3. The high sensitivity and stability, low cost and simplicity in the fabrication procedures of the proposed immunosensor provide potential applications for clinical immunoassays.
研究领域:
生物化学
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纳米医学
·
电化学分析
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DOI
高影响因子
ELECTROANALYSIS
·
2025
·Vol. 37
·期 3
·e12037
·
IF 2.3
Jiajia Wang,Chun Kan,Mengping Gao,Baokang Jin
In this paper, a water-soluble highly electrochemical signal cadmium telluride (CdTe)/cadmium selenide (CdSe)/polyaniline nanocomposite was developed through a fast and convenient method, and then the nanocomposite-modified glassy carbon electrode was prepared for the determination of alpha-fetoprotein (AFP). This aptasensor was constructed by covalently immobilizing NH 2 -functionalized AFP-specific aptamer on nanocomposite with plenty of carboxylic groups. This electrochemical biosensor via the layer-by-layer method could evidently increase the steric hindrance of the sensing electrode and effectively depress the electron transfer, leading to obviously decreased current intensity. The ultrahigh sensitivity of this immunoassay is derived from the two primary reasons as follows. First, the CdTe/CdSe multiple-sensitized and cosensitized structure could maximize speed of charge transfer processes between electrodes and the electroactive species, dramatically promote electron transfer, and effectively inhibit the electron–hole recombination, resulting in the significantly enhanced electrochemical current intensity of the sensing electrode. Second, the electrocatalytic oxidation of K 3 Fe(CN) 6 , which makes the CdTe/CdSe change from a lower-energy to higher-energy states (CdTe/CdSe QDs)*, reduces the activation energy of the reaction and the (CdTe/CdSe QDs)* more likely to oxidize, accelerating the transfer of electrons. Scanning electron microscope, transmission electron microscope, and X-ray photoelectron spectroscopy were used to characterize the material. Electrochemical impedance spectroscopy was used to observe the loading process of the material. Differential pulse voltammetry was used as a method of measurement. The immunosensor exhibited a wide linear range from 1.0 to 10.0 μg/mL for target AFP detection, with a low detection limit of 1.0 pg/mL (S/N = 3). To evaluate the analytical reliability, reproducibility, specificity, and stability, the proposed immunosensor was applied to human AFP-spiked serum samples, and acceptable results were obtained, indicating that the method can be readily extended to other bioaffinity assays of clinical or environmental significance.
研究领域:
生物医学工程
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分析化学
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DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 70
·56583-56589
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IF 3.84
Yulan Wang,Dan Wu,Yong Zhang,Xiang Ren,Yaoguang Wang,Hongmin Ma,Qin Wei
In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP). Thanks to the simple and versatile layer-by-layer self-assembly technology, the facile and firm immobilization of 2D graphene nanosheets (GS), 3D copper oxide nanoflowers (CuO NFs) and 0D gold nanoparticles (Au NPs) was achieved in the fabrication of the proposed immunosensor. A synergic effect amongst the electrocatalytic activity, conductivity and biocompatibility of GS, CuO NFs and Au NPs could provide high sensitivity for the label-free electrochemical immunosensor. The detection mechanism was based on monitoring of the electrocatalytic current response change towards the reduction of hydrogen peroxide (H2O2) when an immunoreaction occurred on the surface of the GS–CuO NFs–Au NPs modified electrode. Under optimal conditions, the designed immunosensor exhibited a wide linear range from 10−5 ng mL−1 to 102 ng mL−1 with a low detection limit of 5.3 fg mL−1 for AFP. It also displayed an electrochemical performance with good reproducibility, selectivity and stability, which could provide potential application in the clinical diagnosis of other tumor markers.
研究领域:
生物医学工程
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分析化学
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肿瘤标志物检测
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Liyan Zhang,Chuan Fan,Min Liu,Fengjuan Liu,Shanshan Bian,Shuyue Du,Shuyun Zhu,Hua Wang
Catalytic hemin was first remolded with biomineralized gold to form Hemin-Au core and further encapsulated into the Tb metal organic framework (MOF) matrix. The so obtained Hemin-Au@MOF composites could possess high environmental stability and especially double catalysis activities of enhanced peroxidase-like and gold catalysis. Steady state kinetics studies indicate that Hemin-Au@MOF could present much higher intrinsic catalysis and substrate affinity (lower Michaelis constants) than native hemin and even natural peroxidase . Furthermore, the catalytic composites were employed for labeling antibodies towards the sandwiched immunoassays for alpha-fetoprotein (AFP) as a model biomarker of cancers. The results indicate that the Hemin-Au@MOF-based immunoassays could be conducted by two signal amplification ways of hemin-catalyzed chromogenic reaction and gold-catalyzed silver staining, enabling the detection of AFP in blood with the level down to 0.020 ng mL −1 . Importantly, the proposed fabrication protocol combining the gold biomineralization and MOF encapsulation routes may be extended for the fabrication of a variety of enzymes or enzymatic mimics with the improved environmental stability and multiple catalysis activities for the wide catalysis applications.
研究领域:
生物化学
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医学诊断
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材料科学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 28
·6664-6670
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IF 7
Wei Wan,Xiangling Ren,Junrui Tan,Longfei Tan,Changhui Fu,Qiong Wu,Zengzhen Chen,Jun Ren,Zhongbing Huang,Xianwei Meng
Janus particles have been considered suitable for biomedicine owing to their asymmetric structure and unique properties. Although Janus particles have been applied in biosensing for dual-mode sensing, there are almost no reports for the detection of multiple indicators. In fact, many patients require different diagnoses, such as the examination of hepatogenic diseases in diabetics. Here, a Janus particle based on SiO2 was synthesized using a Pickering emulsion method. A novel strategy for detecting glucose and alpha-fetoprotein (AFP) based on different principles using this Janus particle was then constructed as a detection platform. Composed of adjustable dendritic silica loaded with gold nanoclusters (Au NCs) and glucose oxidase (GOx) and spherical SiO2 coupled with AFP antibody, this Janus fluorescent probe achieved the double detection of glucose and AFP. With the protection of dendritic silica, the enzyme temperature stability was enhanced. Moreover, the low limit of detection for glucose (0.5 μM in PBS and 2.5 μM in serum) and AFP (0.5 ng mL−1) illustrated the feasibility of the application of the Janus material in integrated detection. This work not only supported the use of a Janus fluorescent probe as a detection platform toward glucose and AFP but also showed the potential of Janus particles in integrated detection in the future.
研究领域:
生物医学工程
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纳米技术
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分析化学
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生物传感器
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DOI
PubMed
高影响因子
Micro & Nano Letters
·
2020
·Vol. 15
·期 3
·125-129
·
IF 0.975
Chulei Zhao,Kaihang Cao,Chaoyun Ma,Wenjun Li,Yiju Song,Xiuwen Qiao,Chenglin Hong
In this experiment, a Cu 2 O spherical nanoparticle was reduced by hydrothermal reaction to prepare a label-free electrochemical immunosensor based on a novel signal amplification strategy. Multi-walled carbon nanotubes are a platform that combines cuprous oxide with carbon nanotubes. Nanogold was modified on the surface of cuprous oxide nanospheres by electrostatic adsorption to further amplify the electrochemical signal and link-supported anti-alpha-fetoprotein (AFP). Under optimal conditions, the proposed immunosensor has a wide linear range from 0.001 to 40 ng/ml, with a detection limit as low as 0.33 pg/ml ( S / N = 3). Furthermore, the proposed label-free immunosensor has been used to determine AFP in human serum with satisfactory results. At the same time, it has good reproducibility, acceptable selectivity and long-term stability, and has broad application prospects in biometric analysis.
研究领域:
分析化学
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纳米科技
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生物化学
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生物医学工程
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电化学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 6
·4653-4660
·
IF 3.201
Peipei Chen,Ting Wang,Xiangli Zheng,Dong Tian,Fangquan Xia,Changli Zhou
A convenient, highly efficient and ultrasensitive electrochemical immunosensor was developed using in situ deposited silver nanoparticles (Ag NPs) as tracing tags for fetoprotein (AFP) detection. The function of the immunosensor was achieved by a co-catalytic effect on the surface of nanocomposites containing polyamidoamine (PAMAM) dendrimers coated with C60 (PAMAM-C60 NPs) and gold nanoparticles (Au NPs). On the surface of Au@PAMAM-C60 NPs labeled with an antibody (Ab2), via a sandwich-type immunoreaction, silver ions in solution were reduced to metallic Ag NPs. The electrochemical signal of the in situ deposited Ag NPs could be utilized to monitor the immunoreaction. Under optimum conditions, the peak current of the Ag NPs increased with increasing AFP concentration in the range from 0.1 pg mL−1 to 10 ng mL−1, with a detection limit of 0.03 pg mL−1. Importantly, the designed electrochemical immunoassay strategy exhibited excellent sensitivity, stability, selectivity, and reproducibility.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
医学诊断
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产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Jing Li,Dali Liu,Donglei Zhou,Long Shao,Xu Chen,Hongwei Song
In this work, a novel label-free photoelectrochemical (PEC) biosensor based on Au/Cs x WO 3 heterogeneous films with low background noise and excellent sensitive detection of alpha-fetoprotein (AFP) was proposed. We prepared Au/Cs x WO 3 heterogeneous films by different methods including template removal and spin coating . The surface plasmon resonances (SPR) of Au film and Cs x WO 3 semiconductor nanocrystals largely improved the photoelectrochemical properties of the electrodes and enhanced the photocurrent . Under the optimal conditions, the prepared PEC biosensor showed a linear response between photocurrent variation and the logarithm of AFP concentration in the range from 0.01 ng/mL to 500 ng/mL with a low detection limit of 7 pg/mL. In addition, the proposed PEC biosensor had excellent specificity, repeatability, long-term stability and showed satisfactory results in the analysis of human serum samples, which would have great prospect in clinical application and biological analysis in the future.
研究领域:
生物传感器
·
光电化学
·
纳米材料
·
生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
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