PEST MANAGEMENT SCIENCE
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2022
·Vol. 78
·期 11
·4497-4506
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IF 4.462
Yuxin Zhang,Qinghao Liu,Wangcang Su,Lanlan Sun,Hongle Xu,Fei Xue,Chuantao Lu,Renhai Wu
BACKGROUND S -metolachlor (MET) was used to prevent weed infestation in sorghum fields, but inappropriate application could result in phytotoxicity on sorghum. Exogenous gibberellin A 3 (GA 3 ) has been applied for alleviating the phytotoxicity of MET. However, its detoxification mechanism is still not well known. RESULTS Leaf deformity of sorghum caused by 200 mg/L MET was alleviated by treating sorghum shoots with 800 mg/L GA 3 , and the injury recovery rate of growth index was over 73%. More importantly, GA 3 could not accelerate the metabolic rate of MET in sorghum. The result of phytohormone metabolomics showed that endogenous GA 3 content in sorghum decreased by 78.10% with MET treatment, while abscisic acid (ABA) content increased by 120.2%, resulting in 10.3-fold increase of ABA/GA 3 ratio. Content of ABA and GA 3 increased by 11.9- and 21.1-fold with MET and GA 3 treatment, respectively, leading to ABA/GA 3 ratio restoration. Moreover, MET inhibited the expression of genes encoding key enzymes related to GA synthesis including CPS1 , KO2 , KAO , GA20ox1D and ABA8ox gene related to ABA metabolism. The transcription levels of GA metabolism-related genes CYP714D1 and GA2ox were up-regulated by 11.2- and 7.2-fold, while ABA synthesis-related genes NCED and ZEP were up-regulated by 8.0- and 3.0-fold, respectively, with MET and GA 3 treatment. Conclusion In this study, exogenous GA 3 protecting sorghum shoots from MET phytotoxicity was due to supplement the MET-induced GA 3 deficiency by absorbing exogenous GA 3 , and restore homeostasis of ABA and GA 3 by promoting ABA synthesis, which provides novel insights for mechanism of GA 3 alleviating MET phytotoxicity. © 2022 Society of Chemical Industry.
研究领域:
植物生理学
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植物激素研究
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农学
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DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 38
·5087-5094
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IF 3.1
Zepeng Li,Yuxiang Wu,Zijing Li,Binger Yu,Xinyi Mao,Guoqing Shi
Acetochlor is an endocrine disruptor. The acetochlor residue is strongly lipophilic and can be enriched into products during the manufacturing process. In this study, we found that dimethyl-β-cyclodextrin (DM-β-CD) solution could decrease the apparent oil/water partition coefficient (Koil–w) of acetochlor and increase the sensitivity of fluorescence lateral flow immunoassay (LFIA) for acetochlor simultaneously. Based on this, a simple LFIA method for the determination of acetochlor and alachlor residues in vegetable oil was established. The detection process only involves vortex mixing of an oil sample and dimethyl-β-cyclodextrin solution in a 1 : 3 (V/V) ratio, loading the water phase onto the immunoassay strips and reading the results. Under optimized conditions, the LOD for acetochlor in oil was 3.53 ng g−1, and the working range was 12.03–2000.00 ng g−1. The recoveries of spiked samples ranged from 91.69% ± 1.12% to 112.23% ± 2.20%. Meanwhile, the cross reactivity for alachlor was 108.22%, while for other investigated acetochlor analogues it was less than 1%, and the recoveries of alachlor were from 92.90% ± 8.03% to 113.53% ± 3.40%, which indicate that this method can detect acetochlor and alachlor simultaneously. Compared with the traditional detection method, the pre-treatment process of the proposed method is “green” and simple, and can be applied to the on-site rapid detection of acetochlor and alachlor in vegetable oil and can provide inspiration for the detection of other lipophilic pollutants.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2024
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IF 9.7
Wangjing Zhai,Qiqi Guo,Nan Wang,Xueke Liu,Donghui Liu,Zhiqiang Zhou,Peng Wang
Antibiotics are widely detected in farmland, which may influence the environmental behavior and risks of the coexisting pesticide. In this work, the effects of antibiotics on metolachlor transformation in soil-pea and the risk of metolachlor to earthworm were assessed, and the mechanism was explored in view of detoxifying process and oxidative stress. Antibiotics affected not the degradation rate but the metabolic profile of metolachlor. In soil, the content of metabolites oxaloacetic acid (OA) and ethane sulfonic acid (ESA) was decreased and dechlorometolachlor (DCL) was increased by antibiotics. In pea, the accumulation of metolachlor, DCL and ESA was decreased, while OA was increased by antibiotics. The changed transformation of metolachlor affected the risk to earthworm according to risk quote assessment. In further research, it was found that cytochrome P450 (CYP450) enzyme was reduced by 12.3% − 30.4% in soil and 12.4% − 23.6% in pea, which might due to excessive ROS accumulation induced by antibiotics, thus affecting the transformation and metabolite profile of metolachlor in soil–plant system.
研究领域:
环境科学
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农业科学
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土壤科学
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植物科学
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毒理学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Chao Liu,Liwei Chen,Dahu Ding,Tianming Cai
Efficient removal of residual metolachlor (MET), a frequently used herbicide from aquatic system is highly desirable but remains a challenge. In the present study, the emerging sulfate radical based advanced oxidation processes (SR-AOPs) is developed for the degradation of MET from aqueous solution. At the conditions of [MET] = 10 mg L −1 , [Peroxymonosulfate (PMS)] = 3 mM, [Catalyst] = 0.2 g L −1 , pH = 6.5, the degradation process could be accomplished within 40 min and the derived pseudo-first-order kinetic rate constant ( k app ) was 0.11 min −1 . The effects of reaction conditions including pH, oxidant dosage, temperature, concomitant anions and humic acid on the degradation process were comprehensively investigated. Sulfate radical was identified to be the main contributor to the degradation process through radical scavenging and electron paramagnetic resonance (EPR) analysis. The second-order rate constant for reaction of SO 4 − with MET was determined to be 2.17 × 10 9 M −1 s −1 by competition kinetic method. The degradation showed a moderate pH-tolerance. Specifically, alkaline/neutral conditions were beneficial for the MET degradation whilst acidic condition exhibited a detrimental effect on the degradation. Through LC-Q-TOF-MS analysis, eleven aromatic derivatives were detected and their molecular structures were tentatively proposed. A tentative mechanism was proposed based on the detected derivatives. The toxicity test further revealed that the degradation products performed a less toxicity than MET. The results pave an avenue toward the applications of SR-AOPs for MET elimination from aquatic environment.
研究领域:
环境科学
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化学工程
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分析化学
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水处理
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2021
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IF 6.986
Yonghong Zha,Yansong Li,Pan Hu,Shiying Lu,Honglin Ren,Zengshan Liu,Hualin Yang,Yu Zhou
Given the great harm of pesticide residues to the environment and public health, exploring ultrasensitive and low-cost methods for their quantitative analysis becomes intensely necessary. Herein, we proposed a double-functionalized gold nanoparticle (AuNP) probe as a signal amplification immunoassay for the detection of acetochlor (ATC), metolachlor, and propisochlor. The AuNP was modified with IgG and fluorophore-labeled duplex DNA by a polyadenine-based freezing method. The quenched fluorescence can be effectively recovered via duplex-specific nuclease (DSN) with excellent cleaving activity. This approach provided limits of detection (LODs) down to 0.03 ng/mL for ATC, 0.10 ng/mL for metolachlor, 0.14 ng/mL for propisochlor, and 0.08 ng/mL for their mixture. The average recoveries of ATC, metolachlor, and propisochlor were 93.0–106.6% from a corn sample, which are in good agreement with the commercial kit (R2 = 0.9995). This “turn-off” fluorescence immunoassay presents considerable potential in the analysis of chloroacetamide herbicide due to its simple process of probe preparing and ultrahigh sensitivity.
研究领域:
分析化学
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环境科学
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公共卫生
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纳米技术
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分子生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2025
·Vol. 192
·期 5
·1-14
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IF 5.3
Bu Ziyun,Si Qiankang,Lu Xiaolin,Wang Pei,Wu Dezhen,Mei Yingying
A novel monolithic capillary column (AuNPs@CMIP(poly-Chitosan)@capillary) with hybridized chiral molecular imprinted polymers (CMIPs) as stationary phase was prepared using S-metolachlor (S-MET) as template, chitosan as functional monomer, glutaraldehyde as cross-linker, and aminated-gold nanoparticles (NH 2 -AuNPs) as nanocarriers. The capillary electrochromatography (CEC) system constructed by AuNPs@CMIP(poly-Chitosan)@capillary achieved the efficient chiral separation of MET (resolution (Rs) = 9.83) as well as separation of its various structural analogs (Rs > 1.58). In addition, AuNPs@CMIP(poly-Chitosan)@capillary exhibits satisfactory reproducibility and stability. The relative standard deviations (RSDs) of migration times and Rs for intra-day, inter-day, and inter-column were all less than 6.1%, and no significant decline in separation performance was observed after 21 days of storage. NH 2 -AuNPs, with their large specific surface area and stable structure, can provide abundant binding sites and play a supporting role, reducing the deformation and collapse of CMIPs’ cavities, and further ensuring the structural stability and separation performance of the stationary phase. Finally, parameters such as imprinting factor and selectivity factor were calculated by adsorption experiments, and the CEC separation mechanism was discussed. The chitosan CMIPs-based capillary monolithic column developed in this work has rarely been reported and is an innovative attempt, providing a feasible and promising approach for the development of CEC chiral monolithic stationary phases. Graphical abstract
研究领域:
分析化学
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材料科学
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环境科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Xinyu Luo,Ziyi Liu,Linfan Wang,Liwei Gao,Dingfeng Wang,Ting Zhang,Da Chen
Rapid and selective detection of copper ion (Cu 2+ ) and glyphosate (GLY) is crucial to environmental pollution control, especially when using a simple and portable device. In this study, an enzyme-free and visual fluorescence sensor based on nitrogen-doped carbon dots (N-CDs) is successfully designed, featuring strong blue fluorescence and excellent stability. The sensor shows a linear response to Cu 2+ over a wide detection range of 0.048 to 40 μM with a low limit of detection (LOD, 48 nM), resulting in fluorescence quenching at 435 nm. Meanwhile, the rapid enhancement in fluorescence intensity of the quenched N-CDs/Cu 2+ system can be triggered by GLY owing to the strong complexation between Cu 2+ and GLY, with a LOD of GLY as low as 0.24 μM. Furthermore, we develop a smartphone-assisted sensing platform using N-CDs/polyvinyl alcohol films, which captures and analyzes fluorescence images to detect Cu 2+ and GLY with sensitive LODs of 73 nM and 0.32 μM, respectively. Satisfactory recoveries, ranging from 94.0 % to 104.6 %, are achieved in real sample detection, implying the potential application in the field of enzyme-free and on-site pesticides detection.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Lifang Fan,Guifang Liang,Caiyun Zhang,Li Fan,Wenjun Yan,Yujing Guo,Shaomin Shuang,Yingpu Bi,Feng Li,Chuan Dong
In this work, a visible-light-driven photoelectrochemical (PEC) sensing platform was developed based on BiOI nanoflowers/TiO 2 nanotubes (BiOI NFs/TiO 2 NTs) for detection of atrazine (ATZ). The BiOI NFs/TiO 2 NTs p–n heterojunctions synthesized by decorating BiOI NFs on TiO 2 NTs via simple hydrothermal approach exhibit strong visible-light absorption ability, high photocurrent response and PEC activity. Thus BiOI NFs/TiO 2 NTs heterostructures were first explored to act as the photoelectrode for the immobilization of the anti-ATZ aptamer to develop a PEC sensing platform. The design PEC aptasensing platform exhibits prominent analytical performance for determination of ATZ with a low detection limit of 0.5 pM under visible-light irradiation, and displays good selectivity for ATZ in the control experiments. The superior behavior of the sensing platform could be ascribed to the design of the appropriate sensing material with tubular microstructure, excellent PEC response of the photoelectrode, and the large loading amount of aptamer. Meanwhile, the PEC sensing platform was used to determine ATZ in environmental samples and a satisfied result was obtained.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
Frontiers in Environmental Science
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2024
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IF 3.3
Ma Rong,Yu Shuangshuang,Li Yafang,Lin Yan,Ma Xiaodong
Background: With the growing use of nitrogen-containing pesticides in agriculture, their residues in the environment have raised significant public health concerns.Objective: This study aimed to develop a novel PPy-DBSA/IT-SPME coating to enhance the detection efficiency of polar nitrogen-containing pesticides in water matrices.Methods: The preparation conditions were optimized, including pyrrole concentration at 7.0 mol/L, DBSA concentration at 0.014 mol/L, oxidant concentration at 0.35 mol/L, and a coating cycle repeated 10 times. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for detection.Results: The novel coating effectively adsorbed 19 different categories of polar nitrogen-containing pesticides, including sulfonylureas, triazolopyrimidines, diphenyl ether herbicides, benzoylurea insecticides, and phenylurea herbicides, with unadsorbed rates below 10%. The analytical method achieved an average recovery rate of 61.92% to 115.21%, with an RSD below 5.0%. Detection and quantification limits ranged from 0.012 to 0.524 μg/L and 0.127 to 5.243 μg/L, respectively.Conclusion: The developed method is green, efficient, simple, and cost-effective. It offers an environmentally friendly and user-friendly approach for the detection of polar nitrogen-containing pesticide residues, demonstrating good recovery and precision. This method holds potential for wide application in environmental monitoring and food safety.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 8
·1280
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IF 4.7
Yifei Sun,Yan Tang,Zetao Chen,Miaoxiu Ge,Wei Xiong,Luhong Wen
Abuse of herbicides in food safety is a vital concern that has an influence on the sustainable development of the world. This work presents, a modified ionization method with separation of the sample and carrier gas inlets, which was utilized for efficient ionization and analyte transfer of herbicides in crops. The working parameters of voltage, injective distance, desorption temperature, and the carrier gas flow rate were optimized to achieve the high efficiency of the transfer and ionization of the analyte. When it was applied in the analysis of herbicides in laboratory, the method exhibited excellent performance in achieving the quantitative detection of herbicides in solutions and residues spiked in an actual matrix with a limit of quantification of 1–20 μg/kg and relative standard deviations of less than 15%. Although a simple QuEchERS process was used, the programmable heating platform ensured efficient gasification and transfer of the target analyte, with the advantages of high speed and selectivity, avoiding the noted matrix effect. The method exhibited a relatively acceptable performance by using air as the discharged gas (open air). It could be used to monitor herbicide residues in the growth stage via on-site non-destructive analysis, which obtained low LODs by dissociating the herbicides from the crops without any pretreatment. It showed great potential for the supervision of the food safety market by achieving non-destructive detection of crops anytime and anywhere. This finding may provide new insights into the determination of pesticide emergence and rice quality assessment.
研究领域:
分析化学
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食品安全
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环境科学
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DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2019
·Vol. 103
·期 1
·193-198
·
IF 1.65
Zhang Wenjie,Wang Jun,Wang Jinhua,Zhu Lusheng,Lv Ning,Wang Rui,Ahmad Zulfiqar
The widespread use of pharmaceutical and personal care products (PPCPs) has attracted much attention and the impact of PPCPs on indigenous microbial communities has become increasingly important in recent days. Five common PPCPs, including doxycycline (DOX), ciprofloxacin (CIP), triclocarban (TCC), carbamazepine (CBZ), and sulfadimidine (SMZ), were selected and their effects on soil microbial respiration were studied at concentrations of 0, 0.2, 1, 5, 25 and 50 mg/kg. The results of this study indicate that the effect of five common PPCPs on soil microbial respiration was dose- and time- dependent. At low concentrations (0.2 and 1 mg/kg), CBZ and SMZ exhibited an activation effect on microbial soil respiration at 1 day (58.02%, 26.39% and 1.54%, 1.76% at 0.2 and 1 mg/kg respectively), while DOX showed inhibition for all tested concentrations at 1 day of incubation. At high concentrations (25 and 50 mg/kg) CIP and SMZ showed an inhibitory effect (− 69.13%, − 80.86% for 25 and 50 mg/kg, respectively), while TCC and CBZ exhibited stimulatory effect (38.07%, 9.64% and 4.06%, 12.18% at 25 and 50 mg/kg, respectively) at 1 day of incubation. Our findings indicate that the effect of tested PPCPs on soil microbial respiration had an inhibitory or stimulatory effect based on the dose and extent of time.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
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IF 6.393
Fuyou Du,Lingshun Sun,Qiulian Zen,Wei Tan,Zhenfang Cheng,Guihua Ruan,Jianping Li
A simple and rapid fluorescence “on-off-on” approach for sensing Hg 2+ and paraquat was designed by using nitrogen doped graphene quantum dots (N-GQDs) and N-GQDs/Hg 2+ system, respectively. It was found that the fluorescence of N-GQDs solution was quenched in the presence of Hg 2+ ions (turn off), and the quenched N-GQDs/Hg 2+ system was recovered after addition of paraquat (turn on), due to the higher affinity of paraquat and Hg 2+ than that of N-GQDs and Hg 2+ that resulting into the release of Hg 2+ from the surface of N-GQDs and the fluorescence recovery of N-GQDs. The developed fluorescence “on-off-on” approach exhibited high selectivity and sensitivity for detection of Hg 2+ and paraquat. Under the optimum experimental conditions, good linearities were achieved over the range of 0–4.31 μM for Hg 2+ and 0.05–2.0 μg mL −1 for paraquat, respectively. The detection limits for Hg 2+ and paraquat were 23 nM and 19 μg L −1 , respectively, and the recoveries of Hg 2+ and paraquat spiked in real samples ranged from 95% to 105.8%. Results of this study suggested that the highly selective and sensitive fluorescence “on-off-on” approach can be used to the detection of Hg 2+ and paraquat in real environmental water samples.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）