FOOD CHEMISTRY
·
2024
·
IF 8.5
Jinkui Wang,Tong Bu,Qianqian Cui,Zizhe Li,Qing Xu,Zhixian Jiang,Yexuan Mao,Xianqing Huang,Lianjun Song,Dapeng Peng,Xiya Zhang
Simultaneous detection of carbofuran (CBF) and 3-hydroxy carbofuran (3-OH-CBF) in fruits and vegetables is important due to their high toxicity and widespread use in pest control. However, most lateral flow immunoassay (LFA) approaches only detection CBF. To overcome this limitation, two haptens, 6-((2,2-dimethyl-2,3-dihydrobenzofuran-7-yl)oxy)hexanoic acid and 6-((((2,2-dimethyl-2,3-dihydroben zofuran-7-yl)oxy)carbonyl)amino)hexanoic acid, named H1 and H2, were designed for broad-spectrum antibody detection of CBF and 3-OH-CBF. The highly specific monoclonal antibody (mAb) 3F4 based on hapten H1 only recognized CBF, while broad-spectrum mAbs 1D3, 6B7 and 6E8 based on hapten H2 recognized both compounds. The carbon bond to the hydroxyl group of 3-OH-CBF adopts a 28.55° angle relative to the same carbon bond of CBF, explaining the specificity of the H1-based mAb against CBF. After optimizing the antigen-antibody combination, a sensitive colloidal gold-LFA (CG-LFA) strip was constructed based on mAb 1D3 and H2-BSA for CBF and 3-OH-CBF detection following simple sample pretreatment. The established LFA had a limit of detection of 0.49–6.63 ng/mL, and adequate recoveries (69.3–105.7 %) for CBF and 3-OH-CBF in 26 fruit and vegetable samples.
研究领域:
环境科学
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食品安全
·
免疫学
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DOI
PubMed
高影响因子
CHINESE CHEMICAL LETTERS
·
2021
·
IF 6.779
Yuan Liu,Gaojian Yang,Taotao Li,Yan Deng,Zhu Chen,Nongyue He
An improved ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX) was applied to select aptamers against carbaryl. After nine selection rounds, a highly enriched ssDNA pool was obtained. The Apta3 was demonstrated as the optimal aptamer. In order to facilitate the modification of aptamer, the Apta3 was further truncated with the dissociation constant ( K d ) of 0.364 ± 0.055 μmol/L and a fluorescent aptasensor was developed. The linear range for carbaryl was from 100 nmol/L to 1500 nmol/L, with the limit of detection was as low as 15.23 nmol/L. Besides, the biosensor was validated for the carbaryl spiked real samples, and the recoveries were between 97.7% and 107.3%.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 41
·4077-4084
·
IF 3.532
Zerong Long,Shilin Shen,Hui Yuan
Carbamate (CB) pesticides possess potential carcinogenic and mutagenic activities towards humans even at very low dosages. Thus, broad-specificity probes with high sensitivity and speed are needed for multiple CB determination. This study is the first to focus on Dy3+ ions-coordinated self-assembly on a silica core using a surface imprinting procedure, for the simultaneous fluorometric detection of residues of metolcarb (MC) and pirimicarb (PC) insecticides. A simple and mild solvothermal method was applied for the preparation of fluorescent imprinted microspheres starting from 1,10-phenanthroline (Phen)-ligated Dy3+ ions to guide imprinted self-assembly of chitosan (CTS), glutaraldehyde (GA), and two carbamate pesticides (MC and PC) on the silica surface by means of coordinate bonds and hydrogen bonds. The as-prepared microspheres displayed strong fluorescence emissions via the antenna effect derived from the Phen ligand and the Schiff base oligomers for sensitizing the Dy3+ ions. An expanded in-depth mechanism study was performed on the fluorescence enhancement involving Förster resonance energy transfer (FRET) from the pesticides (donor) to the acceptor. A linear increase in fluorescence at 483 nm for MC and 574 nm for PC upon the imprinted microspheres was observed under the same 350 nm excitation wavelength. Moreover, the quantitative recognition process could be carried out simultaneously and tolerate strong distractions both from five other similar carbamate insecticides and from complicated matrices (e.g., an extract of Chrysanthemum morifolium Ramat). The detection limit was 4 ng mL−1 with a range of 10–60 ng mL−1 for MC and 0.4 ng mL−1 with a range of 1–30 ng mL−1 for PC. Further characterization of the material, including TEM, SEM, XPS, and FTIR, Raman, and fluorescence spectra, verified that the Dy3+ ions play a decisive role in promoting imprinted self-assembly around the silica core. Hence, a novel polynuclear Ln–organic imprinted probe having high selectivity, stability, and sensitivity for the detection of two carbamate insecticides is presented in this study.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Shujuan Chen,Jing Han,Yiting Zhu,Xinyue Zhang,Chi Zheng,Lihong Ma,Shuliang Liu,Yong Yang,Likou Zou,Li He,Jianlong Li
The residue of carbaryl in food is a threat to human health. In this study, activated soybean shell biochar (A-SBC) was used as a carrier, methacrylic acid (MAA) was used as a functional monomer, and carbaryl was used as a template molecule to synthesize the activated biochar surface molecularly imprinted polymer (A-SBC@MIP). The synthesized A-SBC@MIP was characterized by SEM, FT-IR, XRD and XPS techniques, and then applied as adsorbent for carbaryl removal. The adsorption capacity of A-SBC@MIP for carbaryl was 8.6 mg‧g −1 and the imprinting factor was 1.49 at the optimum ionic strength and pH. The kinetic and isothermal data indicated that it had fast mass transfer rate and high binding capacity(Qmax=47.9 mg‧g −1 ). A-SBC@MIP showed good regenerative properties and the adsorption of carbaryl was excellent in its structural analogues. A solid-phase extraction (SPE) column composed of A-SBC@MIP was developed for the detection of rice and corn under optimized conditions, with recoveries of 93–101% for the spiked carbaryl. The limit of detection (LOD) of the method was 3.6 μg‧kg −1 with good linearity (R 2 =0.994) in the range of 0.01–5.00 mg‧L −1 . The results show that the developed MIPs-SPE can enrich carbaryl from food samples as a specific and cost-effective method.
研究领域:
环境科学
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食品科学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 23
·64352-64362
·
IF 5.8
Qian Cheng,Ma Jie,Wu Qiong
As a typical carbamate pesticide, methomyl was once widely used in agriculture for its excellent broad-spectrum insecticidal effect. However, due to its high toxicity, long half-life, and difficult degradation properties, it poses a serious challenge to water environment pollution. In this study, an electrode-free discharge microwave-induced plasma technology was used to rapidly and efficiently degrade methomyl in aqueous solution. In this experiment, the statistical design of experiments (DOE) was adopted to optimize the plasma degradation parameters. Under the optimized parameters ( P = 140 W, D = 0 mm, R = 0.5 L/min), 78.4% removal of 50 mg/L of methomyl was achieved after 8 min. The optical emission spectrometry and free radical detection experiments showed that the active substances generated by the collision reaction between plasma and water molecules occurring at the gas–liquid interface were the key factors to exert the degradation effect. The degradation rate of methomyl decreased by 73.2% after the addition of tert-butanol (OH burster), while it decreased by only about 12.0% after the addition of peroxidase. These implied that ∙OH was largely responsible for methomyl degradation. In addition, based on the detected intermediates, possible degradation mechanisms and pathways were analyzed.
研究领域:
环境科学
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农药污染治理
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DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 4
·2127-2136
·
IF 3.108
Mengwei Li,Zhendong Zhao,Xiaodan Wu,Wenjun Zhou,Lizhong Zhu
In recent years, biochar has been well-documented in the literature as a promising soil amendment for the sorption and immobilization of organic contaminants in contaminated soils via its organic functional groups. In this study, original and demineralized cow manure biochars were applied with the aim to evaluate the impact of their mineral component on adsorption and competitive adsorption of oxytetracycline and carbaryl. Adsorption increased greatly for oxytetracycline but decreased slightly for carbaryl after demineralization. Mineral components significantly enhanced overall adsorption of carbaryl but slightly inhibited that of oxytetracycline to biochars, because the dominant sorption mechanisms of oxytetracycline and carbaryl on demineralized biochars were established as pore-filling effects and specific-site sorption, respectively. Competitive adsorption was observed in the binary-solute sorption system, where oxytetracycline outcompeted carbaryl on original biochars, however, carbaryl showed a greater competitive adsorption to oxytetracycline on demineralized biochars. Furthermore, demineralization treatment weakened the competition strength of oxytetracycline to carbaryl but enhanced that of carbaryl to oxytetracycline. This work demonstrated that the demineralization treatment altered the physicochemical properties of biochars and the dominant sorption mechanisms of oxytetracycline and carbaryl, leading to their different changes on competition strength. The results are useful for the application of biochars in the remediation of combined pollution in agricultural soils.
研究领域:
环境科学
·
土壤修复
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DOI
高影响因子
FOOD CHEMISTRY
·
2013
·
IF 3.334
MingYan Wang,JunRao Huang,Meng Wang,DongEn Zhang,Jun Chen
A novel nonenzymatic sensor based on cobalt (II) oxide (CoO)-decorated reduced graphene oxide (rGO) was developed for the detection of carbofuran (CBF) and carbaryl (CBR). Two well-defined and separate differential pulse voltammetric peaks for CBF and CBR were obtained with the CoO/rGO sensor in a mixed solution, making the simultaneous detection of both carbamate pesticides possible. The nonenzymatic sensor demonstrated a linear relationship over a wide concentration range of 0.2–70 μM ( R = 0.9996) for CBF and 0.5–200 μM ( R = 0.9995) for CBR. The lower detection limit of the sensor was 4.2 μg/L for CBF and 7.5 μg/L for CBR ( S / N = 3). The developed sensor was used to detect CBF and CBR in fruit and vegetable samples and yielded satisfactory results.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 18
·4221-4229
·
IF 3.286
Long Zerong,Shen Shilin,Lu Yi,Lan Wei,Chen Jia,Qiu Hongdeng
Herein, rare-earth europium doped in Gd 2 O 3 @SiO 2 -based molecularly imprinted polymer (MIP) composite nanospheres with a multilayer core-shell structure was successfully prepared via a facile and versatile layer-by-layer assembly strategy of combination with sol-gel, hydrothermal, and surface imprinting procedure. The rare-earth Gd 2 O 3 :Eu 3+ was embedded into the inner portion of the imprinted polymer which was well-suited for fluorescent monitoring carbaryl selectively. Results showed that the recognition process of the nanosensor for carbaryl was fast and reached dynamic equilibrium at ca. 20 min. The fluorescence intensity ( F 0 / F ) is linearly related to the concentration of carbaryl [ Q ] within the range of 16–80 μg mL −1 , and the linear equation is F 0 / F = 0.8909 − 9.775 × 10 −4 [ Q ] ( R = 0.9963) with 10 μg mL −1 as the detection limit. Competition experiments showed that other analogues (methomyl, aldicarb, and isoprocarb) have nearly no interference in the detection of carbaryl. Moreover, this MIP nanosensor was successfully applied to detect carbaryl in green tea samples without pretreatment. The study afforded an efficient and desirable fluorescence sensor for carbaryl detection in a complicated matrix, which hopefully will be used for biomedical/chemical sensing recognition.
Graphical abstract
研究领域:
分析化学
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材料科学
·
环境科学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lijuan Zhao,Faqiong Zhao,Baizhao Zeng
In this paper, a novel sensitive and selective molecularly imprinted electrochemical sensor was presented for the determination of carbaryl (1-naphthyl methylcarbamate). The sensor was constructed by coating a glassy carbon electrode (GCE) with chitosan-AuPt alloy nanoparticles (CS-AuPtNPs), graphene-ionic liquid-nano Au (GR-IL-Au), and followed by electrodepositing carbaryl imprinted poly(p-aminothiophenol) (p-ATP) film. The CS-AuPtNPs and GR-IL-Au composites played the roles to immobilize monomer p-ATP and to improve the electrochemical response; [Fe(CN)6] 3- /[Fe(CN)6] 4- acted as electrochemical probe. The mechanism of the fabrication process and several important parameters controlling the preparation and electrochemical response of sensor were discussed and optimized. Under the optimal conditions, a linear range from 0.030 μM to 6.0 μM for the detection of carbaryl was obtained. The sensitivity was 4.0 μA/μM mm2 and the detection limit was 8.0 nM (S/N=3). The resulting sensor displayed good selectivity and stability towards carbaryl. It was successfully applied to the determination of carbaryl in practical samples and the recovery was 96 - 105%.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 19
·3130
·
IF 5.561
Juanli Du,Hao Wu,Xu Jing,Yonghe Yu,Zhisheng Yan,Jianhai Zhang
Developing a simple and effective method for measuring carbaryl residues in food is urgent due to its widespread use and the associated health risks in agriculture, as well as various defects in existing detection techniques. The COF (TpPa-NH2)@Fe3O4nanocomposite (amino modification) was synthesized via a two-step method and used as an adsorbent for the extraction of carbaryl from food samples in this study. The results indicated that COF (TpPa-NH2)@Fe3O4can rapidly and successfully capture carbaryl directly from samples via π–π stacking and hydrophobic interactions, achieving maximum adsorption within 5 min under a small adsorbent quantity using a fluorescence spectrophotometer. Under the optimized conditions, carbaryl exhibited good linearity in the range of 0.2–120 µg·kg−1, and the limit of detection was 0.012 µg·kg−1. The recoveries of the samples were 96.0–107.4%. This method has broad application prospects for the monitoring of carbaryl in food.Keywords:magnetic solid-phase extraction;COF (TpPa-NH2)@Fe3O4nanocomposite;fluorescence spectroscopy;carbaryl;food samples
研究领域:
食品安全
·
分析化学
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2012
·
IF 2.656
Xia Sun,Ying Zhu,Xiangyou Wang
In this paper, a novel label-free amperometric immunosensor for the detection of carbofuran residues was developed. The deposited gold nanocrystals (DpAu)/4,4′-thiobisbenzenethiol (DMDPSE) multilayers ({DpAu/DMDPSE} n ) membranes were used for modifying Au electrode to fabricate amperometric immunosensor. DpAu/DMDPSE multilayers were modified alternatively to form multiple membranes by layer-by-layer self-assembly technology. The sensitive steps of surface modification were characterized by cyclic voltammetric (CV) and electrochemical impedance spectroscopy (EIS), respectively. The immunoreaction between anti-carbofuran monoclonal antibody and carbofuran directly triggered a signal via different pulse voltammetry (DPV). Under the optimized conditions, a linear relationship between the relative change in peak current of DPV (%Δ I ) and the logarithm of carbofuran solution was obtained in the range from 0.1 to 1.0 × 10 6 ng/mL with a detection limit of 0.06 ng/mL. The advantages of the immunosensor were exhibited in its wider linear range, better reproducibility, stability, selectivity and regeneration. Using lettuces, cabbages, green peppers, tomatoes, Chinese chives and strawberries as model samples, acceptable recovery of 82.0–109.2% was obtained. The proposed method was proven to be a feasible quantitative method for carbofuran analysis, which may open a new door for ultrasensitive detection of carbofuran residues in vegetables and fruits.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 18
·7611-7620
·
IF 3.288
Phung Nhat Minh,Van-Tuan Hoang,Ngo Xuan Dinh,Ong Van Hoang,Nguyen Van Cuong,Dang Thi Bich Hop,Tran Quoc Tuan,Nguyen Tien Khi,Tran Quang Huy,Anh-Tuan Le
Reduced graphene oxide-wrapped silver nanoparticles (Ag@rGO) were synthesized by a simple photochemical process with the GO nanosheets as both a stabilizing and reducing agent. The synthesized Ag@rGO nanohybrids were used as a localized surface plasmon resonance (LSPR) colorimetric sensor for the detection of chromium(VI) and carbaryl. For chromium(VI), the result showed a color change from yellow to colorless and linear-dependence of the absorption intensity on the concentration of Cr(VI) in the range 0.1–25 μM. For carbaryl, the calibration curve presented a high level of linearity in the range of 0.1–50 μM corresponding to color changes from yellow to purple. The limits of detection (LODs) for Cr(VI) and carbaryl were found to be 31 nM and 42 nM, respectively. The novel Ag@rGO nanohybrids will become an excellent platform for colorimetric probes in the detection of Cr(VI) ions and carbaryl or other heavy metal ions/pesticides.
研究领域:
材料科学
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环境科学
·
分析化学
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产品关联
DOI
高影响因子
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