JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2023
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IF 2.6
Xinyi Lü,Xiaotong Yang,Xiaoli Wu,Jidong Wang,Zhong Zhao
In this paper, the solubility of menadiol diacetate in nine alcohols (methanol, ethanol, n-propanol, i-propanol, n-butanol, i-butanol, n-amyl alcohol, i-amyl alcohol, and cyclohexanol) and five n-alkyl acetates (methyl acetate, ethyl acetate, n-propyl acetate, n-butyl acetate, and amyl acetate) was experimentally measured with the aid of a laser dynamic device at temperatures from 283.15 to 323.15 K. The solubility of menadiol diacetate as a function of temperature was regressed in terms of three semiempirical solubility models (Apelblat, van’t Hoff, and λh), two activity coefficient solubility models (Wilson and NRTL), and the Jouyban model. The thermodynamic mixing properties (mixing Gibbs energy, entropy, and enthalpy) of the solutions were estimated in terms of the measured solubility data and the regressed parameters of the Wilson model.
研究领域:
药学
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物理化学
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DOI
高影响因子
JOURNAL OF MICROBIOLOGICAL METHODS
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2018
·
IF 1.701
Yu-Ke Cen,Jian-Guang Lin,Jun-You Wang,Zhi-Qiang Liu,Yu-Guo Zheng
A colorimetric assay has been developed for quantitative analysis of active biomass of Fusarium fujikuroi , based on the reduction of the tetrazolium salt 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) when menadione was present as an electron coupling agent. The optimum assay-conditions were set as 200 μg/ml XTT, 5 μM menadione and one-hour reaction time. Under these settings, the produced formazan displayed a linear relationship with F. fujikuroi biomass. This method was subsequently applied to evaluate the cell growth behavior, which showed a positive correlation with the carbon source consumption and gibberellin biosynthesis under the industrial fermentation conditions. Our results showed that the XTT-menadione assay is a valuable tool in analyzing the industrial fermentation process of F. fujikuroi , especially when the medium contains insoluble and complex components.
研究领域:
微生物学
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生物技术
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真菌生物学
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DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 10
·2354
·
IF 4.2
Jia-Le Zhou,Hao-Han Chen,Jian Xu,Ming-Yu Huang,Jun-Feng Wang,Hao-Jie Shen,Sheng-Xiang Shen,Cheng-Xian Gao,Chao-Dong Qian
Background:Staphylococcus aureusis a common pathogenic microorganism in humans and animals. Type II NADH oxidoreductase (NDH-2) is the only NADH:quinone oxidoreductase present in this organism and represents a promising target for the development of anti-staphylococcal drugs. Recently, myricetin, a natural flavonoid from vegetables and fruits, was found to be a potential inhibitor of NDH-2 ofS. aureus. The objective of this study was to evaluate the inhibitory properties of myricetin against NDH-2 and its impact on the growth and expression of virulence factors inS. aureus. Results: A screening method was established to identify effective inhibitors of NDH-2, based on heterologously expressedS. aureusNDH-2. Myricetin was found to be an effective inhibitor of NDH-2 with a half maximal inhibitory concentration (IC50) of 2 μM. In silico predictions and enzyme inhibition kinetics further characterized myricetin as a competitive inhibitor of NDH-2 with respect to the substrate menadione (MK). The minimum inhibitory concentrations (MICs) of myricetin againstS. aureusstrains ranged from 64 to 128 μg/mL. Time–kill assays showed that myricetin was a bactericidal agent againstS. aureus. In line with being a competitive inhibitor of the NDH-2 substrate MK, the anti-staphylococcal activity of myricetin was antagonized by MK-4. In addition, myricetin was found to inhibit the gene expression of enterotoxin SeA and reduce the hemolytic activity induced byS. aureusculture on rabbit erythrocytes in a dose-dependent manner. Conclusions: Myricetin was newly discovered to be a competitive inhibitor ofS. aureusNDH-2 in relation to the substrate MK. This discovery offers a fresh perspective on the anti-staphylococcal activity of myricetin.
研究领域:
微生物学
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药理学
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生物化学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2020
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IF 7.182
Wenfeng Ni,Zhiming Zheng,Hui Liu,Peng Wang,Li Wang,Han Wang,Xiaowen Sun,Qiang Yang,Hengfang Tang,Genhai Zhao
Prenyltransferase NovQ immobilized carboxymethyl cellulose magnetic nanoparticles (NCMNs) were successfully synthesized via a valuable approach integrated from nanocomposite preparation, and applied for the production of vitamin K 2 using menadione hydroquinol and dimethylallyl diphosphate (DMAPP) as substrates. To investigate the interaction between nanoparticles and NovQ, we characterized the nanocomposite, and revealed that carboxymethyl cellulose (CMC) and Fe 3 O 4 formed a core-shell structure to absorb NovQ in the reaction systems, resulting from the high affinity of immobilized materials. Meanwhile, NCMNs with excellent pH and temperature tolerance, enhanced prenylated activity, and improved stability were found. Molecular docking analysis was also conducted to justify the contribution of multiple amino acids and effect of nanoparticles on catalytic properties of NovQ. Taken together, our study introduces a promising strategy to prepare magnetic nanoparticles and improve the performance of catalyst, which aims for opening new orientations for synthesis of magnetic nanoparticles used for prenyltransferase immobilization.
研究领域:
纳米技术
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生物化学
·
酶学
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DOI
PubMed
高影响因子
Biomaterials Science
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2020
·Vol. 8
·期 21
·5955-5968
·
IF 6.183
Fangying Yu,Yun Zhu,Yupeng Liu,Guoxi Qiu,Xuwei Shang,Tingting Meng,Hong Yuan,Fuqiang Hu
The active targeting strategy has achieved inspiring progress for drug accumulation in tumor therapy; however, the insufficient expression level of many potential receptors poses challenges for drug delivery. Poly-γ-glutamic acid (γ-pGluA), a naturally occurring anionic biopolymer, showed high affinity with tumor-associated gamma-glutamyl transpeptidase (GGT), which localized on the cell surface and exhibited intracellular redox homeostasis-dependent expression pattern; thus, GGT was utilized for mediating endocytosis of nanoparticles. Herein, GGT-targeting nanopolyplexes (γ-pGluA-CSO@Fe3+, PCFN) consisting of cationic chitosan and GGT-targeting γ-pGluA blended with iron ion were constructed to load reactive oxygen species-induced menadione (MA) and doxorubicin, which were utilized to investigate the mechanism of GGT up-regulation. Briefly, the pretreated PCFN/MA induced an intracellular oxidative stress environment, which facilitated adjusted up-regulated GGT expression and boosted tumor targeting. Subsequently, the destroyed redox homeostasis sensitized tumors for synergistic therapy. The innovative strategy of augmenting active targeting by disturbing intracellular redox homeostasis offers insight for the application of γ-pGluA-derived nanopolyplexes.
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Junxi Liu,Yan Liu,Yuheng Zheng,Haoyu Wang,Jinglin Wang,Yu Zhang,Kaiping Wang
SCLP remained intact in the stomach and small intestine after oral administration. • SCLP was degraded into oligosaccharide fragments in the cecum and colon. • The intestinal metabolism of SCLP was gut microbiota-dependent. • Phocaeicola vulgatus and Bacteroides uniformis were involved in SCLP metabolism. This study aimed to investigate the intestinal metabolic characteristics and mechanisms of the pectic polysaccharide isolated from the medicinal plant Smilax china L. (SCLP). Firstly, in vitro simulated digestion confirmed that SCLP remained stable in simulated digestive fluids. Subsequently, in vivo real-time tracking of intestinal metabolism based on fluorescent labeling revealed that SCLP maintained its prototype in the small intestine and began to be degraded into fragments (Mw < 4000 Da) upon reaching the cecum and colon, where it was retained for prolonged periods. Pseudo-sterile mouse experiments indicated the mediating role of gut microbiota in SCLP metabolism. Furthermore, metagenomic sequencing suggested that SCLP increased the proportion of polysaccharide utilization loci (PULs) from Phocaeicola vulgatus and Bacteroides uniformis , elevated the gene numbers of carbohydrate-active enzymes (CAZymes) including GHs, GTs and CBMs, and activated pathways of carbohydrate metabolism. Finally, in vitro bacterial culture study verified the degradation and utilization of SCLP by Phocaeicola vulgatus and Bacteroides uniformis . In summary, this work elucidates the intestinal metabolic profile of SCLP, providing valuable insights for its further development and utilization.
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PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Wenjia Liu,Ying Wang,Xiangyu Hao,Yangxun Wang,Xiaofan Zhang,Jinchao Zhang,Kun Ge,Guoqiang Zhou
Triple-negative breast cancer (TNBC) is associated with rapid progression and poor sensitivity to monotherapies, necessitating combination strategies to enhance tumor immunogenicity, reprogram the tumor microenvironment (TME), and convert immunologically "cold" tumors into "hot" ones. In this study, we develop a pH-responsive, pyroptosis-inducing nanoreactor, ZnS/ZIF-90:Fe/Vk3 (ZZFV), designed to elicit potent antitumor immunity. Under acidic conditions, ZZFV rapidly degrades, releasing Zn 2+ , Fe 2+ , H 2 S, and Vk3. H 2 S promotes glucose uptake and consumption while downregulating cytochrome c oxidase subunit IV (COX IV) expression, whereas Zn 2+ suppresses lactate dehydrogenase (LDH) activity, collectively disrupting glucose metabolism and exacerbating intracellular acidification. Meanwhile, NAD(P)H quinone oxidoreductase 1 (NQO1), highly expressed in tumor cells, catalyzes the conversion of Vk3 to H 2 O 2 , which is subsequently converted to highly toxic •OH through Fe 2+ -mediated Fenton reactions. Additionally, Zn 2+ and H 2 S synergistically disrupt mitochondrial function, thereby amplifying reactive oxygen species (ROS) production. Excessive ROS triggers Caspase-1-dependent pyroptosis, leading to the liberation of damage-associated molecular patterns. These events promote dendritic cell (DC) maturation, robust T-cell activation, M1 macrophage polarization, and expansion of central memory T-cell populations, thereby conferring durable immune protection. Consequently, ZZFV demonstrates potent antitumor efficacy, effectively overcoming immune tolerance in TNBC and achieving robust, sustained antitumor immunity.
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DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Lei Lei,Wenbin Dai,Jinchao Zhao,Angfeng Jiang,Haisheng Peng,Qiao Jin,Xiaojing Li,Zhe Tang
Photodynamic therapy (PDT) is a promising cancer therapy modality by generating reactive oxygen species (ROS) and triggering immunogenic cell death. However, the therapeutic effect of PDT is strongly limited by tumor hypoxia and immunosuppressive landscape. Herein, a pH-sensitive nanosized covalent–organic polymer (COP), composed of the photosensitizer porphyrin and pyruvate kinase inhibitor vitamin K3 (VK3), is designed to overcome these issues. The signal transducer and activator of transcription 3 (STAT3) inhibitor WP1066 is further encapsulated into COPs to form a WP1066-loaded COP (TVW). As an inhibitor of pyruvate kinase, VK3 can reduce intracellular oxygen consumption by inhibiting the glycolytic pathway, leading to the alleviation of the tumor hypoxic microenvironment. The relief of tumor hypoxia by VK3 enhances photodynamic cytotoxicity by generating more ROS. Meanwhile, STAT3 acts as a major regulator of PD-L1, a key inhibitor that promotes immune escape. WP1066 effectively inhibits the expression of STAT3 and reduces PD-L1 expression, thereby significantly inhibiting tumor immune escape and enhancing antitumor efficacy in a synergistic manner. The antitumor capacity of photodynamic immunotherapy is extensively investigated in a murine subcutaneous hepatocellular carcinoma model. This photo-immunotherapy may provide an effective combination regimen for the efficient treatment of solid tumors such as hepatocellular carcinoma.
研究领域:
肿瘤学
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生物医学工程
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免疫学
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DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-18
·
IF 15.7
Li Wanqian,Whiteway Malcolm,Hang Sijin,Yu Jinhua,Lu Hui,Jiang Yuanying
Azole effectiveness against candidiasis can be compromised by Candida albicans resistance and tolerance, and unfortunately, few clinically useful compounds can enhance azole antifungal activities. We find that the amino acids V234, F235 and L238 of Erg11 are critical for its interaction with Ncp1, and the Ncp1-Erg11 association is important in azole response. Ellipticine and its analog phiKan 083 block this Erg11-Ncp1 interaction by targeting Ncp1, and boost antifungal effects of fluconazole in vitro and in vivo. A series of steps influencing this process—an initial elevation in reactive oxygen species, leading to protein oxidation and misfolding in the endoplasmic reticulum (ER) that causes ER stress. This stress leads to Ca 2+ release from the ER, mitochondrial Ca 2+ accumulation and dysfunction, increased ROS production, and apoptosis of C. albicans cells. Overall, disrupting the Erg11-Ncp1 interaction in C. albicans can serve as a useful approach to enhancing the antifungal properties of azoles.
研究领域:
微生物学
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分子生物学
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药理学
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生物化学
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医学真菌学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2025
·
IF 4.5
Renxiang Yuan,Shuqian Yang,Ding Wang,Caini Yu,Peirong Li,Qi Jiang,Yangqian Luo,Tingting Meng,Fuqiang Hu,Tong Yu,Yupeng Liu,Hong Yuan
Ischemia-reperfusion (IR)-induced acute kidney injury (AKI) is a life-threatening form of AKI frequently observed in clinical settings, such as hemorrhagic shock, kidney transplantation, and cardiovascular surgery. Targeting renal antioxidant therapy may represent an effective strategy against IR-induced AKI. Bilirubin (BR), a specific superoxide anion scavenger, faces limitations in clinical application due to its poor water solubility and hepatic metabolism pathway. In this study, we prepared bilirubin nanocrystals (BRNCs), which demonstrated potent antioxidant effects in vitro experiments. To enhance renal targeting, BRNCs were encapsulated in poly(ethylene glycol) (PEG)-modified lipid nanoparticle (BRNC-LNP). In vivo distribution studies revealed that BRNC-LNP effectively circumvented hepatic metabolism and exhibited enhanced renal accumulation compared to BRNC. In vivo efficacy evaluation revealed superior therapeutic outcomes with BRNC-LNP versus BRNC in AKI mice. These findings suggest that BRNC-LNP may serve as a promising therapeutic strategy for IR-induced AKI by reducing the level of hepatic metabolism and promoting renal targeting.
研究领域:
纳米医学
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药剂学
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肾脏病学
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抗氧化治疗
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Xinyue Huang,Lei Jiang,Lin Yuan,Wensi Ma,Xintao Cui,Jiaxuan Liu,Xiuling Ji,YujiaoTu
Pesticides are chemicals widely utilized in various industries, particularly agriculture, to manage pests and enhance crop yields. However, the excessive use of pesticides, such as phoxim, poses threats to the environment, food safety, and human health. Thus, it is crucial to use pesticides judiciously, limiting their application to what is necessary. Fluorescent probes have garnered attention for their cost-effectiveness, straightforward preparation, and high sensitivity, positioning them as promising candidates for detecting phoxim. In this context, orange ratiometric fluorescent carbon dots were synthesized using rhodamine B and NaOH as precursors through a hydrothermal method. The synthesized Rh B Na CDs were then purified via dialysis, thin-layer chromatography, and chromatographic columns to develop an accurate and precise ratiometric fluorescent probe. This nanoprobe demonstrated excellent linear ranges for detecting phoxim levels in various food samples. It exhibited a low detection limit of 4.486 nM for phoxim concentrations ranging from 0 to 25 μM and can accurately detect concentrations between 25 and 250 μM. Moreover, it proved sensitive in identifying phoxim content in samples of red grapes, green grapes, tomatoes, and pitayas.
研究领域:
分析化学
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环境科学
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食品科学
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纳米技术
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农药检测
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Tong Yu,Yeqing Wang,Yi Kong,Jianwei Wang,Hong Yun,Yunchao Wang,Fuqiang Hu,Zhongxin Zhu,Hong Yuan
Drug delivery system (DDS) is an important branch of pharmaceutics. Rational modification of the physicochemical properties of DDSs can further improve their targeting efficiency. Herein, a bionic DDS is reported for AKI that is scRNA-seq-guided, custom-designed, and prepared, targeting the Key Cell Subtype for Pathological Progression (KCS-PP) of acute kidney injury (AKI). Specifically, scRNA-seq is utilized to identify a specific renal tubular epithelial cell subtype (PTIs) as the KCS-PP for AKI from numerous cellular subtypes. Additionally, specific cell adhesion molecules (VCAM1 and ICAM1) are identified as the Targeting Drug Delivery Mediators (TDDMs) for PTIs from a list of 1000 marker genes of PTIs. Based on this progress, PTI-targeting bionic DDS, named BRNCs@AMMOs is custom-designed and prepared, and used them for AKI treatment in vitro and in vivo. In vitro, BRNCs@AMMOs shows that its adhesion ability in PTI model cells is 3.2 times that in normal cells. In vivo, 6 h after renal pelvis injection, the MFI of BRNCs@AMMOs-DiI in AKI kidneys is 3.7 times that of sham kidneys. The findings demonstrate that BRNCs@AMMOs exhibits prolonged retention in PTI model cells and AKI kidneys. Overall, the custom-designed and prepared PTI-targeting bionic DDS, may promote the customized design and preparation of DDSs for different diseases and targets, is reported.
研究领域:
药剂学
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药物递送
·
分子医学
·
纳米技术
·
转化医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）