FOOD CHEMISTRY
·
2024
·
IF 8.5
Biao Jia,Baoshan He,Renli Liu,Jinping Yang,Wenjie Ren,Zhiguang Suo,Yiwei Xu
The conductivity of Zn-MOF-on-Co-MOF synthesized by one-pot method is improved by searching for the optimum carbonization temperature, which overcomes the limitation of traditional MOF. In order to further enhance electron transfer, the mesoporous PtPdCo trimetal was introduced, which provided considerable load capacity for methylene blue (MB) and reverse complementary DNA (sDNA), and also showed excellent catalytic activity for MB. In this study,the conductivity of aptasensor was improved by modifying carbonized MOF as the base material. In the presence of fipronil (FIP), Apt tended to bind to FIP and was separated from the electrode surface, so that the remaining single-stranded complementary DNA (cDNA) were hybridized with signal probe (MB@PtPdCo/sDNA) and amplified the electrochemical signal. By modifying the substrate, competitive amplification strategy and signal probe material to catalyze signal molecules, the sensor performs multiple signal amplification, and the electrical signal is significantly improved. The sensor has a wide detection range 100 pg/mL to 100 μg/mL and a low detection limit of 76 pg/mL.
研究领域:
分析化学
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环境科学
·
食品安全检测
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 12
·2757-2763
·
IF 5.227
Xinying Zhang,Zhiwei Zhang,Chujing Yang,Ziheng Hu,Yingying Chen,Lingyan Feng
Signal amplification strategies increase the complexities of biosensors while improving the response signals. Herein, a novel electrochemical biosensor was developed based on a DNA hydrogel for sensitive analysis using microRNA-21 (miRNA-21) as a detection model. Poly C sequences combined with C–Ag(I)–C hydrogel formed a DNA hydrogel by the unique interaction between the cytosines and silver ions. Thus, with a three-way conjunction structure of DNA, this C–Ag(I)–C hydrogel was constructed as a novel biosensor for the detection of miRNAs. With the assistance of this hydrogel, numerous silver ions gathered around DNA strands, which would amplify the signal. Under these conditions, the silver ions produced distinct square wave voltammetry oxidation peak currents. This electrochemical biosensor we designed exhibited a great linear relationship for the logarithm of the concentration of miRNA-21 from 1 fM to 100 pM with a detection limit of 0.117 fM. Furthermore, our sensors were able to differentiate miRNA-21 from its homologous family with satisfactory responsiveness in the dilute bovine serum system.
研究领域:
生物传感技术
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分子诊断
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分析化学
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纳米医学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jing Liu,Yan Ping Chen,Penglin He,Ziyu Ding,Yun Guo,Songhe Cui,Chao Ma,Zhiping Xie,Sun Xia,Yin Zhang,Ye Liu,Yuan Liu
Hexanal is considered as an important volatile compound indicator for the assessment of freshness and maturity of foods. Therefore, sensitive and stable monitoring of hexanal is highly desired. Herein, an efficient receptor immobilization strategy based on ZIF-8@ Single-walled carbon nanotube (SWCNT) and nanosomes-AuNPs/Prussian blue (PB) was proposed for the development of olfactory biosensors. ZIF-8@SWCNT as dual support materials provided a high density of active sites for nanosomes loading. Moreover, the co-electrodeposition of nanosomes-AuNPs and PB on the sensor interface effectively amplified the electrochemical signal and maintained the activity of the receptor. The combination of ZIF-8@SWCNT with AuNPs/PB imparts excellent sensing performance of the biosensor with a wide detection range of 10 −16 –10 −9 M, a low detection limit of 10 −16 M for hexanal, and a long storage stability of 15 days. These results indicate that our biosensor can be a powerful tool for versatile applications in food and other related industries.
研究领域:
食品科学
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分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Yanyu Lin,Rongkai Shen,Nannan Liu,Huan Yi,Hong Dai,Jianhua Lin
Here, a simple electrochemical biosensor was proposed based on the specific recognition between trypsin and peptide. Initially, NiCo 2 O 4 -PAMAM nanocomposite was casted on the bare electrode to achieve the electrochemical signal amplification in 0.1 mM [Ru(NH 3 ) 6 ] 3+ solution owing to the great electronic conductivity and high electrochemical activity induced by the special structure of NiCo 2 O 4 nanosheets (Ni 3+ cations in octachedral sites of the Co 3 O 4 ). Subsequently, a declined electrochemical signal was obtained when g-C 3 N 4 labeled peptide composites were anchored on the electrode. However, after trypsin was added into solution and incubated with the biosensor, the electrochemical signal was re-promoted. Therefore, the as-synthesized biosensor could realize the sensitive detection of trypsin by virtue of the specific recognition between trypsin and peptide. As a result, the developed peptide-based exhibited a linear range from 10 −10 to 10 −4 mg mL −1 with an ultralow detection limit of 10 −10 mg mL −1 , providing sensitive analytical performance and acceptable application potential in clinical test and disease diagnosis due to its high stability, excellent selectivity , acceptable reproducibility and accurate signal output.
研究领域:
生物传感技术
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2023
·Vol. 77
·期 12
·7577-7587
·
IF 2.2
He Jintao,Zhang Baozhong,Tian Panpan,Li Ying,Liu Xiaolong,Ma Xinyue,Lin Min,Zhu Huina,Chen Hanyu,Li Liping,He Baoshan
Cadmium (Cd), a typical heavy metal not essential for the human body, can cause harm to the liver and kidneys upon exposure. Hence, rapid cadmium detection in the environment is of utmost importance. This research presents an effective approach for detecting divalent cadmium ions (Cd 2+ ) using a sensitive dual hairpin (HP) electrochemical aptasensor. The aptasensor incorporates AuPd/Fe-MOF as signal labels and Au/Cu 2 O as the substrate material, and the rapid detection of divalent cadmium ions (Cd 2+ ) was based on catalytic hairpin self-assembly (CHA), as the recognition strategy. Au/Cu 2 O increased the specific surface area of the electrode and provided abundant sites for capturing the complementary deoxyribonucleic acid (CDNA) probe. The electrochemical signal is then amplified through synergistic catalytic hydrogen peroxide with AuPd/Fe-MOF, thereby enhancing the aptasensor's sensitivity. Moreover, the dual HP design effectively reduces the likelihood of non-specific capture and minimizes false positives. In this study, various analytical techniques were utilized to characterize the material and evaluate the aptasensor's performance. The morphological characteristics of the material were observed using scanning electron microscopy (SEM). Energy-dispersive spectroscopy (EDS) and X-ray diffraction (XRD) were employed to analyze the elemental composition and distribution of the material, respectively. The electrochemical behavior of the sensor was studied using cyclic voltammetry (CV), while electrochemical impedance spectroscopy (EIS) helped understand the aptasensor's assembly process. Furthermore, the aptasensor's performance was assessed using differential pulse voltammetry (DPV). Under the optimized experimental conditions, the constructed sensor demonstrated effective detection of Cd 2+ in the concentration range of 10 –4 –10 µM, with a remarkably low detection limit of 2.27 × 10 –5 µM. The feasibility of the sensor was validated by successfully detecting Cd 2+ in real water samples. .
研究领域:
分析化学
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环境科学
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材料科学
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电化学
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产品关联
DOI
高影响因子
Micro & Nano Letters
·
2020
·Vol. 15
·期 3
·125-129
·
IF 0.975
Chulei Zhao,Kaihang Cao,Chaoyun Ma,Wenjun Li,Yiju Song,Xiuwen Qiao,Chenglin Hong
In this experiment, a Cu 2 O spherical nanoparticle was reduced by hydrothermal reaction to prepare a label-free electrochemical immunosensor based on a novel signal amplification strategy. Multi-walled carbon nanotubes are a platform that combines cuprous oxide with carbon nanotubes. Nanogold was modified on the surface of cuprous oxide nanospheres by electrostatic adsorption to further amplify the electrochemical signal and link-supported anti-alpha-fetoprotein (AFP). Under optimal conditions, the proposed immunosensor has a wide linear range from 0.001 to 40 ng/ml, with a detection limit as low as 0.33 pg/ml ( S / N = 3). Furthermore, the proposed label-free immunosensor has been used to determine AFP in human serum with satisfactory results. At the same time, it has good reproducibility, acceptable selectivity and long-term stability, and has broad application prospects in biometric analysis.
研究领域:
分析化学
·
纳米科技
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生物化学
·
生物医学工程
·
电化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）