ANALYTICAL SCIENCES
·
2024
·Vol. 40
·期 6
·1081-1087
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IF 1.8
Mei Xuqiao,Zeng Zhenhua,Xu Wenxin,Yang Huicong,Zheng Yuanhai,Gao Haimin,Wu Chuncai,Zheng Yanping,Xu Qiaoli,Wang Guowei,Xu Yuhuang,Wu Ayang
CA125 (carbohydrate antigen 125) is an important biomarker of ovarian cancer, so developing effective method for its detection is of great significance. In the present work, a novel sandwich-like electrochemical immunosensor (STEM) of CA125 was constructed by preparing nanoribbon-like Ti 3 C 2 T x MXenes (Ti 3 C 2 T x NR) to immobilize primary antibody (PAb) of CA125 and UIO-66-NH 2 MOFs structure to immobilize second antibody (SAb) and electroactive toluidine blue (Tb) probe. In this designed STEM assay, the as-prepared Ti 3 C 2 T x NR nanohybrid offers the advantages in large surface area and conductivity as carrier, and UIO-66-NH 2 provided an ideal platform to accommodate SAb and a large number of Tb molecules as signal amplifier. In the presence of CA125, the peak currents of Tb from the formed STEM structure increase with the increase of CA125 level. After optimizing the related control conditions, a wide linear range (0.2–150.0 U mL −1 ) and a very low detection limit (0.05 U mL −1 ) of CA125 were achieved. It’s thus expected the developed STEM strategy has important applications for the detection of CA125. Graphical abstract
研究领域:
生物医学工程
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分析化学
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肿瘤学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Mengmeng Zhang,Ximei Hu,Lisha Mei,Li Zhang,Xiao Wang,Xiaochen Liao,Xiuwen Qiao,Chenglin Hong
Conventional electrochemical immunosensors use electroactive materials often suffer from leakage of electroactive materials and signal instability. To solve this problem, this paper proposes an electrochemical immunosensor based on MOF-235 adsorption aggregation signal amplification strategy for the sensitive detection of PSA. Methylene blue (MB) is a cationic dye with redox properties. It has electrochemical reversibility, high electron transfer rate and good biocompatibility. Therefore, it is often used as an electroactive substance in electrochemical immunosensors. However, the non-specificity and poor stability of MB will inevitably distort the diagnostic results and limit its application. In this experiment, MOF-235, a fluffy metal porous MOF material with two carboxyl groups has been prepared and it has a large specific surface area and good biocompatibility. Meanwhile, it can generate electrostatic attraction with the amino groups on MB, and firmly fix methylene blue on the MOF material, which can effectively prevent the signal leaks of MB during the test, and after being adsorbed, the reunion of MB can be controlled, which is conducive to electron transmission thus amplifying the electrical signal. The produced electrochemical immunosensor has good stability and repeatability, and the concentration of the analyte is directly linear with the magnitude of the electrical signal. Under optimum conditions, the proposed immunosensor shows a wide detection range from 10 pg·mL −1 to 1200 pg·mL −1 with a low detection limit of 3 pg·mL −1 .
研究领域:
生物医学工程
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分析化学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 9
·2006-2011
·
IF 4.726
Na Li,Yaoguang Wang,Wei Cao,Yunhui Zhang,Tao Yan,Bin Du,Qin Wei
The excellent catalytic activity of a novel nanomaterial was introduced to ultrasensitive sandwich-type electrochemical immunosensor applications for detection of carcinoembryonic antigen (CEA). Amino-functionalized multi-walled carbon nanotube (MWCNT-NH2) supported PdPt nanocages were used as catalytic labels of secondary anti-CEA. PdPt nanocages with hollow interiors and porous walls could efficiently enhance electrocatalysis for reduction of H2O2. Meanwhile, with good dispersion, large specific surface area and amino groups, MWCNT-NH2 can immobilize a large amount of PdPt nanocages to improve the sensitivity and amplify the signal of the immunosensor. For the immobilization of primary antibodies, 3-aminopropyltriethoxysilane functionalized graphene sheets were used as transducing materials to modify glassy carbon electrodes and to enhance the electron transfer efficiently. Under the optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.001–20 ng mL−1 with a low detection limit of 0.2 pg mL−1 for CEA. The proposed sensing strategy enriches electrochemical immunoassays and may have a promising application in bioassay analysis.
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 14
·4578
·
IF 4.927
Zheng Wei,Xiaoping Cai,Weifeng Cui,Junping Zhang
A CA19-9 electrochemical immunosensor was constructed using a hybrid self-assembled membrane modified with a gold electrode and applied to detect real samples. Hybrid self-assembled membranes were selected for electrode modification and used to detect antigens. First, the pretreated working electrodes were placed in a 3-mercaptopropionic acid (MPA)/β-mercaptoethanol (ME) mixture for 24 h for self-assembly. The electrodes were then placed in an EDC/NHS mixture for 1 h. Layer modification was performed by stepwise dropwise addition of CA19-9 antibody, BSA, and antigen. Differential pulse voltammetry was used to characterize this immunosensor preparation process. The assembled electrochemical immunosensor enables linear detection in the concentration range of 0.05–500 U/mL of CA19-9, and the detection limit was calculated as 0.01 U/mL. The results of the specificity measurement test showed that the signal change of the interfering substance was much lower than the response value of the detected antigen, indicating that the sensor has good specificity and strong anti-interference ability. The repeatability test results showed that the relative standard deviations were less than 5%, showing good accuracy and precision. The CA19-9 electrochemical immunosensor was used for the actual sample detection, and the experimental results of the standard serum addition method showed that the RSD values of the test concentrations were all less than 10%. The recoveries were 102.4–115.0%, indicating that the assay has high precision, good accuracy, and high potential application value.Keywords:self-assembled membrane;gold electrode;electrochemical immunosensor;CA19-9;gastric cancer
研究领域:
分析化学
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生物传感器
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癌症诊断
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DOI
PubMed
高影响因子
Nanomaterials
·
2021
·Vol. 11
·期 7
·1757
·
IF 5.076
Xiaohua Ma,Dehua Deng,Ning Xia,Yuanqiang Hao,Lin Liu
Nanocatalysts are a promising alternative to natural enzymes as the signal labels of electrochemical biosensors. However, the surface modification of nanocatalysts and sensor electrodes with recognition elements and blockers may form a barrier to direct electron transfer, thus limiting the application of nanocatalysts in electrochemical immunoassays. Electron mediators can accelerate the electron transfer between nanocatalysts and electrodes. Nevertheless, it is hard to simultaneously achieve fast electron exchange between nanocatalysts and redox mediators as well as substrates. This work presents a scheme for the design of electrochemical immunosensors with nanocatalysts as signal labels, in which pyrroloquinoline quinone (PQQ) is the redox-active center of the nanocatalyst. PQQ was decorated on the surface of carbon nanotubes to catalyze the electrochemical oxidation of tris(2-carboxyethyl)phosphine (TCEP) with ferrocenylmethanol (FcM) as the electron mediator. With prostate-specific antigen (PSA) as the model analyte, the detection limit of the sandwich-type immunosensor was found to be 5 pg/mL. The keys to success for this scheme are the slow chemical reaction between TCEP and ferricinum ions, and the high turnover frequency between ferricinum ions, PQQ. and TCEP. This work should be valuable for designing of novel nanolabels and nanocatalytic schemes for electrochemical biosensors.Keywords:electrochemical immunosensor;nanocatalyst;redox cycling;quinone
研究领域:
生物传感
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分析化学
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DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Feifan Qi,Mengdie Wu,Simin Liu,Wendi Mu,Chunyan Wu,Xinshui Ren,Chuang Rui,Fangfang Wu,Dong Chang,Hongzhi Pan
Carbohydrate antigen 199 (CA199) is the most sensitive marker reported for pancreatic cancer, and it is a difficult task to develop a highly sensitive assay for CA199. During the experiment, a ratiometric electrochemical immunosensor for quantitative analysis of CA199 was prepared for NiCo@Fc-MWCNTs-LDH as the electrode sensing surface and 3D-rGOF@Ag/Au as the label of Ab 2 . NiCo@Fc-MWCNTs-LDH not only provide the required signal for the immunosensor, but also have a layered structure to obtain a large specific surface area, which can provide more sites for the placement of biological molecules. rGOF has the advantages of large specific surface area and high porosity, which can adsorb Ag electrochemical probe through redox reaction. The modification of gold nanoparticles can not only enhance the electrical conductivity of nano-composites, but also immobilize more biomolecules to improve the sensitivity of electrochemical sensors. With the beefing up of CA199 concentration, the oxidation peak current of Ag increases and the oxidation peak current of Fc-COOH decreases. The ratio (y = I Ag /I Fc-COOH ) of two different signals was linear with the logarithm of CA199 concentration in a certain value range. Under optimal conditions, the immunosensor showed excellent performance in the concentration range of 0.0001 U/mL to 10 U/mL, and the detection limit was 5.55 × 10 −4 U/mL. The strategy could clearly discriminate between matched and mismatched targets, demonstrating high specifificity. This approach further detects CA199 in human plasma to differentiate pancreatic cancer patients from healthy individuals with high accuracy. This method also provided a new idea for the ultrasensitive quantitative detection of other biomarkers.
研究领域:
生物医学工程
·
肿瘤标志物检测
·
纳米材料科学
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DOI
PubMed
高影响因子
Journal of Analytical Methods in Chemistry
·
2022
·Vol. 2022
·期 1
·5711384
·
IF 2.594
Miao Yu,Ming Liu,Yuan Li
This work describes a label-free electrochemical immunosensor for the sensing of Epstein-Barr virus (EBV) with high sensitivity. First, a monolayer of 1,6-hexanedithiol (HDT) was fabricated on the screen-printed electrode surface by the interaction between sulfur atoms and SPE. AuNPs can be modified on the electrode by the Au-S bond formed between the HDT-free group and Au atom in AuNPs. Protein A is then adsorbed onto AuNPs. Several parameters were optimized. The optimum concentration of protein A is 0.6 mg/mL. The optimum immobilization time for protein A is 90 min. The optimum concentration of antibody is 80 μ g/mL. The optimum immobilization time for antibody is 90 min. Directional immobilization of EBV antibody is achieved by high affinity binding of protein A to the Fc segment of antibody. When antigen specifically binds to antibody, the formation of immune complexes blocks electron transfer of [Fe(CN)6] 4-/3- and is reflected in the detection of cyclic voltammetry/electrochemical impedance spectroscopy. The detection range is 1 pg/mL–l00 ng/mL with a LOD of 0.1 pg/mL. In addition, the proposed sensor exhibited an excellent antiinterference property.
研究领域:
医学检测
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生物传感
✓ 已验证
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Haotian Wu,Gang Zhang,Xiaozhan Yang
An electrochemical biosensor based on iron tetroxide (Fe 3 O 4 )/carboxylated carbon nanotubes (MWCNTs-COOH)/gold nanoparticles (AuNPs) was designed for the detection of alpha-fetoprotein (AFP), which is often used as an important indicator for the early clinical diagnosis of liver cancer markers. The Fe 3 O 4 /MWCNTs-COOH nanocomposite was synthesized by a solvothermal method and it combined with gold nanoparticles (AuNPs) deposited at the constant potential on a glassy carbon electrode to form Fe 3 O 4 /MWCNTs-COOH/AuNPs, which intensifies the electrical signal while the large active sites enable more stable immobilization of the AFP monoclonal antibodies on the electrode. The electrochemical performance of Fe 3 O 4 /MWCNTs-COOH/AuNPs was investigated in detail and the electrochemical response signal after the immune reaction with the AFP antigen-antibody was recorded. The peak current Ip of the response signal is linearly proportional to the lg c AFP in the range of 1 pg mL −1 –10 μg mL −1 , with a detection limit of 1.09034 pg mL −1 and good performance in clinical sample testing. The proposed sensor has shown great application and development potential in clinical medicine field.
研究领域:
生物化学
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纳米技术
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生物医学工程
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临床医学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Yongze Xie,Min Zhang,Qianyun Bin,Shilei Xie,Lu'an Guo,Faliang Cheng,Weizhong Lv
The fast and accurate detection of Carcinoembryonic Antigen (CEA) plays an important role in clinical cancer treatment and therapy. An ultrasensitive photoelectrochemical (PEC) immunosensor for the detection of CEA was constructed using CdSe@BiVO 4 co-sensitized TiO 2 nanorods as photoactive materials. TiO 2 nanorods were assembled on the FTO modified electrode to immobilize capture antibodies. With a sandwich immunoassay format, CEA and signal antibodies labelled CdSe@BiVO 4 were introduced in sequence via specific immunoreaction, and the ultrahigh sensitivity of this immunoassay results from the following three aspects. Firstly, the co-sensitization of BiVO 4 and CdSe extends the absorption range of TiO 2 from ultraviolet region to visible light region, which can adequately utilize the light energy; Secondly, the effective matching of energy levels among TiO 2 , CdSe and BiVO 4 accelerates the separation and transfer of photogenerated electron-hole pairs and significantly improves the PEC performance; Finally, the introduced Au evidently expedites the interfacial electron transfer from TiO 2 to FTO electrode, further resulting in noticeably increased photocurrent . Based on multiple signal amplification strategy, a largely linear detection range from 0.01 ng mL −1 to 50 ng mL −1 with a low detection limit (0.5 pg mL −1 ) were obtained. In addition, the prepared immunosensor with attractive properties provides a promising platform for PEC detection.
研究领域:
生物医学工程
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肿瘤学
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分析化学
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2021
·
IF 5.373
Mengmeng Zhang,Lisha Mei,Li Zhang,Xiao Wang,Xiaochen Liao,Xiuwen Qiao,Chenglin Hong
Electrochemical immunoassays are commonly used to detect biomarkers and Ti 3 C 2 MXene anchored CuAu-LDH two-dimensional hydroxide heterojunctions for dual-mode electrochemical immunosensors were fabricated in this work. Layered double hydroxides have a large surface area, high chemical stability, tunable metal composition and interchangeable anions, however, the insulating nature of LDH further limits its catalytic performance. For this reason, Ti 3 C 2 Mxenes were introduced to improve this problem. 2D layers of Ti 3 C 2 Mxenes with large specific surface area and excellent conductivity have been well proven and widely used. And the surface of Ti 3 C 2 Mxenes (due to the presence of abundant surface functional groups), will facilitate the anchoring of metal ions and the nucleation of LDH. In addition, its excellent electrical conductivity will facilitate the electron transfer between Cu 2+ and Cu + . The immunosensor not only showed a heavy square wave voltammetry (SWV) signal. It also exhibited high electrocatalytic activity for H 2 O 2 redox reactions and improves the sensitivity of the Ampere Current (i-t) detection. The CEA immunosensor developed in this study showed a wide linear response (0.0001–80 ng/mL) and the lowest detection limits (SWV: 33.6 fg/mL and i-t: 45.4 fg/mL S/N = 3). The results confirmed the excellent analytical capability of the immunosensor.
研究领域:
生物医学工程
·
分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yu Wang,Xiaoming Ma
The development and research of electrochemical immunoassay have attracted considerable attention. However, traditional electrochemical immunoassays inevitably require the participation of biological enzymes, which suffer from high cost, poor stability, inconvenient storage and easy inactivation. Herein, we constructed a bio-enzyme-free electrochemical immunoassay system specifically for the detection of aflatoxin B1(AFB1). This method was based on the traditional antigen-antibody immune recognition system while utilizing nanosilver particles (NSP) as signal markers to replace conventional natural enzymes. Subsequently, the labeled NSP was transferred to silver ions in the presence of nitric acid. The concentration of silver ions was detected using anodic stripping voltammetry (ASV), which correlates closely to the concentration of the target. The current intensity measured by the bio-enzyme-free electrochemical sensor exhibited a negative correlation with the concentration of AFB1. Under optimized conditions, the electrochemical sensor was used to detect AFB1 in the dynamic concentration range of 0.01~100 ng/mL, and the limit of detection was 0.4882 pg/mL. The recovery range of AFB1 spiked in corn starch was determined to be between 100.98% and 109.42%, while the relative standard deviation (RSD) range was found to be from 0.34% to 3.82%. These results indicate that the electrochemical immunosensor without biological enzyme labeling has reliable accuracy, and the sensor has a broad application prospect in AFB1 detection.
研究领域:
食品安全
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分析化学
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-12
·
IF 5.3
Ouyang Ruizhuo,Feng Meina,Liu Jinyao,Wang Caihong,Wang Zhongmin,Hu Xin,Miao Yuqing,Zhou Shuang
Rapid and sensitive detection of carcinoembryonic antigen (CEA) is of great significance for cancer patients. Here, molybdenum (Mo) was doped into bismuth oxide (Bi 2 O 3 ) by one-pot hydrothermal method forming porous tremella Bi 2 MoO 6 nanocomposites with a larger specific surface area than the spherical structure. Then, a new kind of hydrangea-like TiO 2 /Bi 2 MoO 6 porous nanoflowers (NFs) was prepared by doping titanium into Bi 2 MoO 6 , where titanium dioxide (TiO 2 ) grew in situ on the surface of Bi 2 MoO 6 nanoparticles (NPs). The hydrangea-like structure provides larger specific surface area, higher electron transfer ability and biocompatibility as well as more active sites conducive to the attachment of anti-carcinoembryonic antigen (anti-CEA) to TiO 2 /Bi 2 MoO 6 NFs. A novel label-free electrochemical immunosensor was then constructed for the quantitative detection of CEA using TiO 2 /Bi 2 MoO 6 NFs as sensing platform, showing a good linear relationship with CEA in the concentration range 1.0 pg/mL ~ 1.0 mg/mL and a detection limit of 0.125 pg/mL (S/N = 3). The results achieved with the designed immunosensor are comparable with many existing immunosensors used for the detection of CEA in real samples. Graphical abstract
研究领域:
生物医学工程
·
分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）