Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Qian Shiwu,Wang Chenyu,Fang Changxiang,Li Meng,Qiao Yan,Hu Weihua
Understanding the extracellular electron transfer (EET) process of electroactive bacteria is of great significance. It is critical yet challenging to differentiate the partial currents from direct (DET) and mediated electron transfer (MET) pathways in the integrated EET current. Herein the EET current of model exoelectrogen is successfully disentangled by using spatiotemporally-resolved oblique-incidence reflection difference (OIRD) technique coupled with polyaniline (PANI)-based dual electrode. The PANI film serves as an electron acceptor to translate the charge information into OIRD signals, enabling mapping of EET current. Upon complete reduction of PANI, the local EET current is switched off, and the soluble mediators are forced to discharge on the nearby PANI electrode, enabling measuring of MET current. In such a way, the DET and MET currents are measured and the average currents from each bacterium are quantified. As-reported method enables successful disentangling the EET current and may offer valuable insights to related research.
研究领域:
微生物电化学
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生物电化学
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纳米技术
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电分析化学
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DOI
PubMed
高影响因子
iScience
·
2023
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IF 5.8
Qilin Yu,Haohao Mao,Bowen Yang,Yahui Zhu,Cheng Sun,Zhiqiang Zhao,Yang Li,Yaobin Zhang
Electrical stimulation has been used to strengthen microbial extracellular electron transfer (EET), however, the deep-seated reasons remain unclear. Here we reported that Bacillus subtilis , a typical gram-positive bacterium capable of extracellular respiration, obtained a higher EET capacity after the electrical domestication. After the electrical domestication, the current generated by the EET of B. subtilis was 23.4-fold that of the control group without pre-domestication. Multiple lines of evidence in bacterial cells of B. subtilis , their cell walls, and a model tripeptide indicated that the polarization of amide groups after the electrical stimulation forwarded the H-bonds recombination and radical generation of protein-like substances to develop extracellular electron transfer via the proton-coupled pattern. The improved electrochemical properties of protein-like substances benefited the trans -cell-wall electron transfer and strengthen extracellular respiration. This study was the first exploration to promote microbial extracellular respiration by improving the electrochemical properties of protein-like substances in cell envelopes.
研究领域:
微生物学
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生物物理学
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电化学
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2024
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IF 9.7
Yuxin Liu,Zhichao Kang,Quanying Wang,Tianye Wang,Ningning Song,Hongwen Yu
Microbial immobilization technology is effective in improving bioremediation efficiency and heavy metal pollution. Herein, Bacillus LD513 with hexavalent chromium (Cr(VI)) tolerance was isolated and immobilized on a novel ferrous disulfide (FeS 2 )/iron nitride (FeN) modified hydrochar (Fe3-SNHC) prepared from waste straws. The prepared Fe3-SNHC-based LD513 (FeLD) significantly improves Cr(VI) adsorption and reduction by 31.4 % and 15.7 %, respectively, compared to LD513 alone. Furthermore, the FeLD composite system demonstrates efficient Cr(VI) removal efficiency and good environmental adaptability under different culture conditions. Microbial metabolism and electrochemical analysis indicate that Fe3-SNHC is an ideal carrier for protecting LD513 activity, promoting extracellular polymer secretion, and reducing oxidative stress . Additionally, the carrier serves as an electron shuttle that accelerates electron transfer and promotes Cr(VI) reduction. Overall, FeLD is an environmentally friendly biocomposite that shows good promise for reducing Cr(VI) contamination in wastewater treatment.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2023
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IF 4.4
Xiaoxue Zhang,Mengmeng Zhong,Rui Zhou,Wenxiu Qin,Youbin Si
Microbial reduction of Se(IV) to Se(0) is extremely important for the detoxification of selenium oxyanion, but the reductive mechanism is still controversial. In this study, the reduction of Se(IV) by Shewanella oneidensis MR-1 and Shewanella putrefaciens was systematically investigated. The results showed that excessive Se(IV) severely inhibited the growth of microorganisms and Se(IV) reduction. The extracellular accumulation of Nano-Se(0) particles was shown to be approximately 250–650 nm in diameter. Riboflavin facilitated Se(IV) reduction, while rotenone , as an electron inhibitor, could completely inhibit Se(IV) reduction, validating the extracellular electron transport process for biosynthesized Nano-Se(0). Importantly, the amorphous Nano-Se(0) synthesized by S. oneidensis MR-1 were mixtures of helical chains and Se 8 rings, while those synthesized by S. putrefaciens were mainly composed of helical chains. FTIR results demonstrated that Nano-Se(0) particles were wrapped by some proteins, organic substances and lipids , exhibiting superior dispersibility and biocompatibility. Biogenic Nano-Se(0) particles can also be considered for technological applications in various fields.
研究领域:
微生物学
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环境科学
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DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2022
·Vol. 26
·期 11
·2435-2443
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IF 2.747
Jiang Demin,Zhu Chenyi,He Yuan,Xing ChengCheng,Xie Kun,Xu Yan,Wang Yuqiao
Anodes play an important role in the extracellular electron transfer (EET) process in microbial fuel cells (MFCs). Herein, polyaniline modified multilayer MXene (PANI-MXene) on carbon cloth was used as an anode to improve the EET efficiency. The PANI-MXene was formed by the electrostatic interaction between the negatively charged groups of MXene and the positively charged protonated amino groups of PANI to enhance the bacterial adhesion and biofilm formation. The MFCs obtained a high maximum power density (737.3 mW·m -2 ) with the efficient EET process. This work provides a meaningful way to decorate anodes using binder-free modifiers for MFCs.
研究领域:
环境科学
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材料科学
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微生物学
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DOI
高影响因子
JOURNAL OF APPLIED ELECTROCHEMISTRY
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2024
·
IF 2.4
Bi Cen,Wen Qing,Chen Ye,Xu Haitao
Microbial fuel cell (MFC) has been regarded as a promising green and clean electricity generation device. The anode plays a crucial role in the electricity generation process within the MFC. This study employed a hydrothermal method for the doping of sulfur onto MXene nanosheets to alleviate the stacking, followed by an in situ polymerization process to fabricate sufur-doped MXene/polypyrrole (S–MXene/PPy) composite hydrogel on carbon felt (CF). The MFC constructed with this anode exhibited the highest power density of 8.05 W m −3 and a maximum output voltage of 646 mV, higher than that of MXene/PPy (623 mV, 6.03 W m −3 ), PPy (594 mV, 4.91 W m −3 ), and unmodified CF (574 mV, 4.05 W m −3 ). This improved performance can be attributed to the reduction of charge transfer resistance in the material while improving the bacteria affinity, leading to enhanced extracellular electron transfer (EET) efficiency. High-throughput sequencing indicates that the excellent biocompatibility significantly promotes the attachment and growth of electroactive microorganisms ( Geobacter , 45.34%) on the S–MXene/PPy anode. This study demonstrates that S–MXene/PPy hydrogel has good potential as an anode from the perspective of MFC electricity generation and microbial analysis. Graphical abstract
研究领域:
环境科学
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材料科学
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微生物学
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DOI
高影响因子
ACS Applied Bio Materials
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2024
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IF 4.6
Jiawei Yang,Pengcheng Xu,Haoming Li,Haichun Gao,Shaoan Cheng,Chaofeng Shen
3D printing of a living bioanode holds the potential for the rapid and efficient production of bioelectrochemistry systems. However, the ink (such as sodium alginate, SA) that formed the matrix of the 3D-printed bioanode may hinder extracellular electron transfer (EET) between the microorganism and conductive materials. Here, we proposed a biomimetic design of a 3D-printed Shewanella bioanode, wherein riboflavin (RF) was modified on carbon black (CB) to serve as a redox substance for microbial EET. By introducing the medicated EET pathways, the 3D-printed bioanode obtained a maximum power density of 252 ± 12 mW/m2, which was 1.7 and 60.5 times higher than those of SA-CB (92 ± 10 mW/m2) and a bare carbon cloth anode (3.8 ± 0.4 mW/m2). Adding RF reduced the charge-transfer resistance of a 3D-printed bioanode by 75% (189.5 ± 18.7 vs 47.3 ± 7.8 Ω), indicating a significant acceleration in the EET efficiency within the bioanode. This work provided a fundamental and instrumental concept for constructing a 3D-printed bioanode.
研究领域:
生物电化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2016
·Vol. 100
·期 12
·5607-5618
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IF 3.376
Li Mu,Su Yinglong,Chen Yinguang,Wan Rui,Zheng Xiong,Liu Kun
The heterotrophic denitrification requires the participation of electrons which are derived from direct electron donor (usually nicotinamide adenine dinucleotide (NADH)), and the electrons are transferred via electron transport system in denitrifiers and then consumed by denitrifying enzymes. Despite the reported electron transfer ability of humic substances (HS), the influences of fulvic acid (FA), an ubiquitous major component of HS, on promoting NADH generation, electron transfer, and consumption in denitrification process have never been reported. The presence of FA, compared with the control, was found not only significantly improved the total nitrogen (TN) removal efficiency (99.9 % versus 74.8 %) but remarkably reduced the nitrite accumulation (0.2 against 43.8 mg/L) and N 2 O emission (0.003 against 0.240 mg nitrogen/mg TN removed). The mechanisms study showed that FA increased the metabolism of carbon source via glycolysis and tricarboxylic acid (TCA) cycle pathways to produce more available NADH. FA also facilitated the electron transfer activities from NADH to denitrifying enzymes via complex I and complex III in electron transport system, which improved the reduction of nitrate and accelerated the transformations of nitrite and N 2 O, and lower nitrite and N 2 O accumulations were therefore observed. In addition, the consumption of electrons in denitrification was enhanced due to FA stimulating the synthesis and the catalytic activity of key denitrifying enzymes, especially nitrite reductase and N 2 O reductase. It will provide an important new insight into the potential effect of FA on microbial denitrification metabolism process and even nitrogen cycle in nature niches.
研究领域:
环境科学
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微生物学
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生物地球化学
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DOI
PubMed
高影响因子
Journal of Cleaner Production
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2022
·
IF 11.072
Dong Feng,Ao Xia,Shuai Wu,Yun Huang,Xianqing Zhu,Xun Zhu,Balakrishnan Deepanraj,Pau-Loke Show,Qiang Liao
Conductive materials may boost the anaerobic digestion of thermal pretreated biomass by overcoming the inhibition effect from by-products generated during the thermochemical pretreatments . The purpose of this study was to explore the influence of magnetite on the anaerobic digestion of furfural, which is found in most biomass hydrolysates . When adding 10 g/l magnetite , the methane yield was enhanced by 125% in comparison to the control. Cyclic voltammetry analysis suggested that the electroactive aggregates were formed after magnetite was added. The microbial community results showed that the Sedimentibacter was significantly enriched (0.2%–18.8%) in the magnetite addition trails; meanwhile, the relative abundance of Methanosaeta was increased by 22.9% compared with the control. Magnetite-amended group of 10 g/l had the lowest hydrogenotrophic methanogens abundance (5.6%) but the highest electrotrophic methanogens abundance (85.3%) than other groups. The energy production efficiency in 10 g/l magnetite addition was 2.3 times higher than that of the control. These evidences indicated that the direct interspecies electron transfer pathway in furfural digestion may be established between Sedimentibacter and Methanosaeta in the presence of magnetite.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Qizi Fu,Chenxi Li,Zirui Liu,Xingyu Ma,Yunhao Xu,Yan Wang,Xuran Liu,Dongbo Wang
Bisphenol A (BPA), as a typical leachable additive from microplastics and one of the most productive bulk chemicals, is widely distributed in sediments, sewers, and wastewater treatment plants, where active sulfur cycling takes place. However, the effect of BPA on sulfur transformation, particularly toxic H2S production, has been previously overlooked. This work found that BPA at environmentally relevant levels (i.e., 50–200 mg/kg total suspended solids, TSS) promoted the release of soluble sulfur compounds and increased H2S gas production by 14.3–31.9%. The tryptophan-like proteins of microbe extracellular polymeric substances (EPSs) can spontaneously adsorb BPA, which is an enthalpy-driven reaction (ΔH = −513.5 kJ mol–1, ΔS = −1.60 kJ mol–1K –1, and ΔG = −19.52 kJ mol–1 at 35 °C). This binding changed the composition and structure of EPSs, which improved the direct electron transfer capacity of EPSs, thereby promoting the bioprocesses of organic sulfur hydrolysis and sulfate reduction. In addition, BPA presence enriched the functional microbes (e.g., Desulfovibrio and Desulfuromonas) responsible for organic sulfur mineralization and inorganic sulfate reduction and increased the abundance of related genes involved in ATP-binding cassette transporters and sulfur metabolism (e.g., Sat and AspB), which promoted anaerobic sulfur transformation. This work deepens our understanding of the interaction between BPA and sulfur transformation occurring in anaerobic environments.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Engineering
·
2025
·
IF 11.6
Wenyue Yan,Baogang Zhang,Yi’na Li,Jianping Lu,Yangmei Fei,Shungui Zhou,Hailiang Dong,Fang Huang
Microbial vanadate (V(V)) reduction is a key process for environmental geochemistry and detoxification of vanadium (V). However, the electron transfer pathways and V isotope fractionation involved in this process are not yet fully understood. In this study, the V(V) reduction mechanisms with concomitant V isotope fractionation by the gram-positive bacterium Bacillus subtilis ( B. subtilis ) and the gram-negative bacterium Thauera humireducens ( T. humireducens ) were investigated. Both strains could effectively reduce V(V), and (90.5% ± 1.6%) and (93.0% ± 1.8%) of V(V) at an initial concentration of 50 mg·L −1 was removed over 10 d incubation, respectively. V(V) was bioreduced to insoluble vanadium (IV), which was distributed both inside and outside the cells. Electron transfer via cytochrome C, nicotinamide adenine dinucleotide, and glutathione played critical roles in V(V) reduction. Metabolomic analysis showed that differentially enriched metabolites (quinone, biotin, and riboflavin) mediated electron transfer in both strains. The aqueous V in the remaining solution became isotopically heavier as V(V) bioreduction proceeded. The obtained V isotope composition dynamics followed a Rayleigh fractionation model, and the isotope enrichment factor ( ε ) was (–0.54‰ ± 0.04‰) for B. subtilis and (–0.32‰ ± 0.03‰) for T. humireducens , with an insignificant difference. This study provides molecular insights into electron transfer for V(V) bioreduction and reveals V isotope fractionation during this bioprocess, which is helpful for understanding V biogeochemistry and developing novel strategies for V remediation.
研究领域:
环境微生物学
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地球化学
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生物化学
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产品关联
DOI
高影响因子
CORROSION SCIENCE
·
2024
·
IF 7.4
Xiangping Hao,Yixin Qian,Yun Bai,Ning Wang,Hongchang Qian,Yuntian Lou,Luning Wang,Dawei Zhang
This study reports the effect of extracellular electron transfer (EET) on biomineralization mediated by Shewanella putrefaciens in damaged coatings under different carbon source conditions. S. putrefaciens was able to obtain energy from metal surfaces to support metabolism via EET when the carbon source was insufficient. Under these conditions, bacteria colonized the coating scratches, resulting in accelerated corrosion instead of mineralization. The EIS results revealed that biomineralization was most significant in the 75% carbon source medium after 7 days of incubation. Interestingly, the pH of the medium increased with an abundance of carbon sources, which promoted biomineralization.
研究领域:
微生物学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）