Biosensors-Basel
·
2022
·Vol. 12
·期 7
·522
·
IF 5.743
Aihua Zhang,Jiaru Fang,Ji Wang,Xi Xie,Hui-Jiuan Chen,Gen He
Cell perforation is a critical step for intracellular drug delivery and real-time biosensing of intracellular signals. In recent years, the nanostraws system has been developed to achieve intracellular drug delivery with minimal invasiveness to the cells. Repeated cell perforation via nano-system could allow delivery of multiple drugs into cells for cell editing, but the biosafety is rarely explored. In this work, a nanostraw-mediated nano-electroporation system was developed, which allowed repeated perforation of the same set of cells in a minimally invasive manner, while the biosafety aspect of this system was investigated. Highly controllable fabrication of Al2O3nanostraw arrays based on a porous polyethylene terephthalate (PET) membrane was integrated with a microfluidic device to construct the nanostraw-electroporation system. The pulse conditions and intervals of nano-electroporation were systematically optimized to achieve efficient cells perforation and maintain the viability of the cells. The cells proliferation, the early apoptosis activities after nanostraw-electroporation and the changes of gene functions and gene pathways of cells after repeated nano-electroporation were comprehensively analyzed. These results revealed that the repeated nanostraw-electroporation did not induce obvious negative effects on the cells. This work demonstrates the feasibility of repeated nano-electroporation on cells and provides a promising strategy for future biomedical applications.Keywords:nanostraw device;nano-electroporation;repeated electroporation;cellular safety;microfluidic device
研究领域:
生物医学工程
·
细胞生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Zishuai Li,Zhengmao Wang,Junmin Pan,Xiaoxiao Ma,Wenpeng Zhang,Zheng Ouyang
Single-cell metabolite analysis plays an important role in biological study. While mass spectrometry is a powerful tool for identification and quantitation of metabolites, the low absolute analyte amounts in single cell and difficulty in sampling represent significant challenges in single cell analysis. In this study, we developed an effective method with a simple sampling procedure for analyzing single cells. A single cell was driven to a capillary tip through electro-migration, followed by releasing the cell contents through electroporation, into a sealed small volume (∼1.5 pL) to prevent dilution. Subsequent mass spectrometry analysis was performed directly with nanoelectrospray ionization. This method was applied for analyzing a variety of cells and monitoring the metabolic changes in response to perturbed cell culturing conditions. This method opens a new avenue for easy and rapid analysis of single cells with high sensitivity.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
DOI
高影响因子
Materials Chemistry Frontiers
·
2025
·
IF 6.4
Lei Xu,Wenjing Lou,Fan Xu,Yujiao Xie,Yue Hu,Liting Xie,Chengyue Zhang,Aochi Liu,Xinyu Miao,Zhiwei Hou,Wenyuan Ma,Qiyu Zhao,Jie Lin,Aiguo Wu,Tianan Jiang
Irreversible electroporation (IRE) has emerged as a promising therapeutic modality for pancreatic cancer. However, traditional IRE techniques rely on high-voltage electric fields and require precise alignment of multiple electrodes, which complicates the procedure and increases associated risks. To address these challenges, we developed a novel “bipolar” IRE electrode that combines both the cathode and anode into a single device, simplifying the procedure and potentially reducing operational risks. Additionally, we incorporated a conductive metal–organic framework (MOF) to enhance the electric field distribution of the electric field, thereby improving the efficacy of tumor ablation. Mechanistic studies revealed that this combined approach induces tumor cell apoptosis and improves the consistency of ablation outcomes. Both in vitro and in vivo experiments demonstrated that the bipolar electrode, in combination with the conductive MOF, achieved a significant apoptosis rate of 59.95% ± 2.41 in vitro and resulted in an 85.77% ± 0.21 reduction in tumor volume in in vivo models, without any adverse effects. This approach provides a more optimized and potentially more effective solution for treating pancreatic cancer.
研究领域:
肿瘤学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Nanotechnology Reviews
·
2023
·Vol. 12
·期 1
·
IF 7.4
Yingzheng Yin,Jie Ding,Yue Cao,Shaozhi Li,Qingbo Ma,Jinyang Li,Xiaoling Xu,David Hui,Zuowan Zhou
It is quite necessary to develop a safe and efficient technique for disinfection of drinking water to avoid waterborne pathogens of infectious diseases. Herein, ZnO nanoarray electrodes with different sizes were investigated for low-voltage and high-efficiency electroporation disinfection. The results indicated that the ZnO nano-pyramid with small tip width and proper length exhibited over 99.9% disinfection efficiency against Escherichia coli under 1 V and a flow rate of 10 mL/min (contact time of 1.2 s). The suitable size of the nanoarray for electroporation disinfection was optimized by establishing the correlation between four kinds of ZnO nanoarrays and their efficiency of electroporation disinfection, which can guide the preparation of next-generation electroporation-disinfecting electrodes.
研究领域:
微生物学
·
环境科学
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Hai Liu,Wei Huang,Yang Yu,Da Chen
Conventional oxidative disinfection methods are usually inefficient to eliminate intracellular antibiotic resistance genes (i-ARGs) due to competitive oxidation of cellular components of antibiotic-resistant bacteria (ARB), resulting in the ubiquitous occurrence of ARGs in drinking water systems. Herein, we developed the strategy of coupling electroporation and electrochemical oxidation on a Co3O4-nanowires-modified electrode to destroy the multiresistant Escherichia coli cells and promote subsequent i-ARG (blaTEM-1 and aac(3)-II) degradation. The lightning-rod effect over nanowire tips can form finite regions with a locally enhanced electric field and highly concentrated charge density, in turn facilitating the electroporation for ARB cell damage and electrochemical reactivity for reactive chlorine/oxygen species generation. Characterization of the ARB membrane integrity and morphology revealed that electroporation-induced cell pores were further enlarged by the oxidation of reactive species, resulting in i-ARG removal at lower applied voltages and with 6–9 times lower energy consumption than the conventional electrochemical oxidation approach with a Co3O4-film-modified electrode. The satisfactory application and effective inhibition of horizontal gene transfer in tap water further demonstrated the great potential of our strategy in the control of the ARG dissemination risk in drinking water systems.
研究领域:
环境科学
·
微生物学
·
水处理工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2022
·Vol. 9
·期 10
·2105240
·
IF 17.521
Huiming Peng,Jian Shen,Xin Long,Xiaoqi Zhou,Jiaqi Zhang,Xina Xu,Teng Huang,Hui Xu,Shuguo Sun,Chun Li,Ping Lei,Heshui Wu,Jun Zhao
Pancreatic cancer is a deadly disease with little response to standard therapies. Irreversible electroporation (IRE) has emerged as a novel ablative technique for the clinical treatment of pancreatic cancer. Combinations of IRE and immunotherapies, including anti-programmed death 1 ( α PD1) immune checkpoint blockade, have shown promising efficacy in both preclinical and clinical studies. However, tumor recurrence remains an obstacle that needs to be overcome. It herein is shown that IRE induces a substantial infiltration of neutrophils into pancreatic tumors. These neutrophils are then polarized into a protumor phenotype by immunosuppressive cues, in particular transforming growth factor β (TGF- β ). Using glutathione-responsive degradable mesoporous silica nanoparticles loaded with SB525334, an inhibitor of TGF- β 1 receptor, it is demonstrated that local inhibition of TGF- β within the tumor microenvironment promotes neutrophil polarization into an antitumor phenotype, enhances pancreatic cancer response to combined IRE and α PD1 therapy, and induces long-term antitumor memory. The therapeutic efficacy is also attributed to tumor infiltration by CD8 + cytotoxic T cells, depletion of regulatory T cells, and maturation of antigen-presenting dendritic cells. Thus, modulating neutrophil polarization with nanomedicine is a promising strategy for treating pancreatic cancer.
研究领域:
肿瘤学
·
纳米医学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
LASER PHYSICS
·
2018
·Vol. 28
·期 7
·075604
·
IF 1.158
Caiyun Li,Huajiang Wei,Yanping Zhao,Guoyong Wu,Huaimin Gu,Zhouyi Guo,Hongqin Yang,Yonghong He,Shusen Xie
The purpose of this study is to illustrate experimentally the optical coherence tomography (OCT) signal slope and diffuse reflectance (DR) spectra of 30% and 80% glycerol combined with electroporation (EP) diffusion in normal, benign and cancerous human gastric tissues in vitro. The results of OCT showed that the permeability coefficients of 80% and 30% glycerol (both with and without EP) have the following trend: human cancerous gastric tissue > human benign gastric tissue > human normal gastric tissue under the same conditions. The permeability coefficient of the 30% glycerol group is larger than that of the 80% glycerol group under the same circumstances; the permeability coefficient of glycerol combined with the EP group is larger than that without the EP group under the same conditions. The permeability coefficient and the reduction of the DR spectra have perfect linear correlation (R2 = 0.9745). The research results suggest that OCT and the DR spectra combined with an optical clearing agent (glycerol) and the EP method can potentially become a powerful tool for the early diagnosis and monitoring of human gastric cancer.
研究领域:
医学影像学
·
生物光学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
Cancer Nanotechnology
·
2025
·Vol. 16
·期 1
·1-38
·
IF 4.8
Abd El-Kaream Samir Ali,Mohamad Abed Elrahman Ahmad,El-Kholey Sohier Mahmoud,Ebied Samia Abd El-Moniem
Background In the realm of cancer treatment, sono–X-ray–photodynamic therapy (SXPDT) has garnered significant interest as a novel therapeutic approach. The essential part of SXPDT is the sensitizer, which under X-ray photon and ultrasound sono-irradiation may transform sono and photo-energy into cytotoxic molecules. Photon absorption, targeting, penetration, and oxygen dependence remain challenges in sono–X-ray–photosensitizer (SXPs) design. Rapid advancements in material science have prompted the creation of several SXPs that create cytotoxic species with great selectivity, safety, and noninvasiveness for the treatment of tumors. The current study aims to provide an advanced method of activated cancer treatment by using electroporation to assist the delivery of Roussin salts porphyrin-based conjugated carbon nanoparticles (EP@RRBP-CNP) for the sono–X-ray–photodynamic prostate cancer (PCa) in vivo and in vitro treatment. Materials and methods Human PCa cells (DU-145) were used in the in vitro study, and the in vivo application groups of the study protocol were Swiss albino mice treated with N-methyl-N-nitrosourea (MNU) / testosterone only; they were not given any treatment to induce PCa. The study treatment protocol started only after PCa induction, and involved daily administration of EP@RRBP-CNP as SXPDT sensitizer whether or not to be exposed to photo–(X-ray) or sono–(US) or a combination of them for 3 min for a period of 2 weeks. Results Indicated that CNP is a useful RRBP delivery mechanism that targets PCa cells directly. Furthermore, EP@RRBP-CNP is a promising SXPS that, when used in conjunction with SXPDT, can be very effective in in vitro treating PCa-DU-145 (in a dose-dependent manner cell viability declined, an increase in the cells population during the G0/G1-phase indicates that the cell cycle was arrested, and an increase in cell population in the Pre-G, autophagic cell death, as well as necrosis and early and late apoptosis, indicate that cell death was induced) and MNU/testosterone-PCa-induced mice in vivo (induced antiproliferative genes, p53, Bax, TNFalpha, caspase 3,9, repressed antiangiogenic and antiapoptotic genes, VEGF and Bcl2, respectively), successfully slowing the growth of tumors and even killing cancer cells, as well as lowering oxidative stress (MDA), improving the functions of the kidneys (urea, creatinine), liver (ALT, AST), and antioxidants (GPx, GPx, GST, CAT, GSH, TAC). SXPDT, the X-ray photo- or sono-chemical RRBP activation mechanism, and the antioxidant capacity of non-activated RRBP can all be linked to this process. Conclusion On the bases of the findings, EP@RRBP-CNP shows a great promise as a novel, efficient selective delivery system for localized SXPDT-activated prostate cancer treatment.
研究领域:
肿瘤学
·
纳米医学
·
生物材料科学
✓ 已验证
DOI
高影响因子
Nano Energy
·
2022
·
IF 19.069
Xinmeng Zhang,Haoqiang Huang,Weinan Zhang,Zulu Hu,Xiang Li,Jia Liu,Gaixia Xu,Chengbin Yang
Urine is the primary source of pathogens (viruses, fungi, and bacteria) in urban sewage. The epidemic spread of these pathogens has a severe threat to human health and the ecological environment. The traditional sewage sterilization methods usually produce harmful by-products and have high energy consumption. These weaknesses restrict the quality of the purified water and economic benefits. Herein, we elaborately fabricated a triboelectric nanogenerator driving nanowires electrode array (T-NEA) system for removing bacteria in the urine. An irreversible electroporation damage results in high-efficiency bacteria inactivation. The sterilization efficiency of T-NEA system is more than 99.9999% in artificial urine contaminated by Escherichia coli ( E. coli ), Staphylococcus aureus ( S. aureus ) and Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa) . Those bacteria could cause epidemic infection in hospital. The electroporation effect on the bacterial membrane was confirmed by SEM imaging and confocal laser scanning microscopy . In addition, it was demonstrated that the radical oxygen species accompanied by T-NEA working could degrade the organic components in artificial urine. After being treated by T-NEA for 30 mins, the reduction rates of the total organic carbon and total nitrogen in the artificial urine reached up to 30.41% and 33.79%. Meanwhile, the degradation degree of urea, uric acid, and creatinine reached 37.39%, 30.50%, 6.25%, respectively. And the degradation mechanism of organic materials in urine was also investigated. This work provided an effective self-powered system which could totally remove the hazardous bacteria and degrade the organics in the urine.
研究领域:
环境科学
·
生物医学工程
·
微生物学
✓ 已验证
DOI
高影响因子
LASER PHYSICS LETTERS
·
2015
·Vol. 12
·期 11
·116003
·
IF 2.458
L P Zhou,G Y Wu,H J Wei,Z Y Guo,H Q Yang,Y H He,S S Xie
In this study, we presented the research of the penetration of zinc oxide nanoparticles (ZnO NPs) (30 and 90 nm), and electroporation (EP) assisted penetration of the ZnO NPs in the human normal colon (NC) and adenomatous colon (AC) tissues studied with optical coherence tomography (OCT) and diffuse reflectance (DR) measurement. The results have shown that the attenuation coefficient of colon tissue after the application of 30 or 90 nm ZnO NPs alone decreased approximately by 28% and 14% for NC tissue, 35% and 22% for AC tissue, respectively; while the attenuation coefficient of colon tissue after combined application of 30 or 90 nm ZnO NPs/EP decreased approximately by 46% and 30% for NC tissue, and 53% and 42% for AC tissue, respectively. The results illustrate EP can significantly increase the penetration of ZnO NPs in the colon tissue, especially in AC tissue. Through the analysis of attenuation coefficient and reflectance intensity of the colon tissue, we find that the accumulation of the ZnO NPs in the colon tissue greatly influenced the tissue optical properties.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Liuxia Li,Yifan Gao,Zihuang Peng,Xun Pei,Ruorong Hua,Fuchang Lin,Rui Zhang,Yi Liu
The influence of pulsed electric fields with different waveforms on extracting valuable compounds from microalgae was investigated. Four different waveforms (rectangle, bipolar rectangle, oscillating superimposed rectangle, and two-step rectangle) with the same amplitude (25 kV/cm) and energy (4 J/pulse) were generated using a pulsed power generator to induce electroporation and component release of three species of microalgae. For Nannochloropsis and Spirulina platensis , the oscillating superimposed rectangular pulse increased the conductivity of microalgal suspensions twice as much as the other waveforms. For Chlorella vulgaris , the protein extraction effect of the oscillating superimposed rectangular pulse was 70 % higher than that of the conventional unipolar rectangular pulse. The pulse waveforms did not show significant differences in the extraction of small molecules, with the carbohydrate extraction index exceeding 0.3 for four waveforms. The results showed that the pulse waveforms were important for extracting large molecules (e.g., proteins). Reasonably designed pulse waveforms for different species of microalgae can significantly improve protein extraction or selectively extract carbohydrates.
研究领域:
生物技术
·
生物工程
·
应用微生物学
·
食品科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
IEEE Photonics Journal
·
2017
·Vol. 9
·期 1
·1-10
·
IF 2.291
Panchun Gu,Huajiang Wei,Guoyong Wu,Zhouyi Guo,Hongqin Yang,Yonghong He,Shusen Xie
The objective of this paper is to evaluate the penetration and accumulation of SiO
2
nanoparticles (NPs) in vitro human placental tissue as a function of NP size and electroporation (EP) and microneedle (MN) treatments. The results show that the penetration and accumulation of SiO
2
NPs has a significant influence on the optical properties. Optical coherence tomography (OCT) monitoring and diffuse reflectance (DR) spectra measurements indicate that 30 nm SiO
2
NPs have a tendency to generate greater signal intensities, while 15 nm NPs penetrated faster and had a higher penetration depth. The reduction in average attenuation coefficients of human placental tissue when the SiO
2
NPs size is constant is found to show the following trend: SiO
2
NPs in combination with EP and MN treatment > SiO
2
NPs in combination with MN > SiO
2
NPs with EP > only SiO
2
NPs. It can be concluded that EP and MN treatments dramatically increase the penetration of SiO
2
NPs into human placental tissue. The results prove that OCT and DR spectra can be combined with EP and MN treatments to establish a theoretical basis for the safe use of SiO
2
NPs on human placental tissue and can potentially become a powerful tool for early diagnosis and monitoring of tissue diseases.
研究领域:
生物医学工程
·
药理学
·
纳米医学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）