FOOD CHEMISTRY
·
2022
·
IF 9.231
Jiaoyong Li,Zongyuan Yang,Yufan Zhang,Boyan Gao,Yuge Niu,Liangli (Lucy) Yu
The tomato pomace, a by-product of tomato processing, was rich in nutrients such as lycopene (Lyc), vitamins, phenols and soluble dietary fibers (SDF). Homogenization combined with enzymatic hydrolysis (HE) was firstly applied to obtain HE-pomace. The yield of Lyc was raised by 57.2% after HE treatment by the optimal condition. The extraction rate of HE-SDF was increased by 73.4%. In order to clarify the relationship between the SDF and the release of Lyc, SDFs were characterized by structural analysis and morphological determination. The results suggested that HE-SDF possessed smaller molecular weight and loose microstructure with shorter chains. It implied that the degradation of dietary fiber led to the release of Lyc molecules. Besides, HE-SDF exhibited stronger capacity of water-holding, glucose adsorption and bile acid binding. In conclusion, HE treatment possessed the potential to be applied as an excellent modification method, which improved the nutritional and economic value of tomato pomace.
研究领域:
食品科学
·
营养学
✓ 已验证
DOI
高影响因子
Stress Biology
·
2023
·Vol. 3
·期 1
·1-16
·
IF 0
Zhao Haipeng,Ding Xiangyu,Chu Xiaomeng,Zhang Haimiao,Wang Xinyu,Zhang Xinwen,Liu Haoqi,Zhang Xiaoying,Yin Ziyi,Li Yang,Ding Xinhua
Gray mold is a destructive disease caused by Botrytis cinerea , a pervasive plant pathogen, which poses a threat to both tomato growth and postharvest storage. The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks. ZNC (zhinengcong), an extract derived from the endophytic fungus Paecilomyces variotii , has been discovered to play a vital role in preventing diverse forms of bacterial infections. Nevertheless, the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear. In this study, we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B. cinerea . The results of both the metabolomic analysis and high-performance liquid chromatography (HPLC) demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin. Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species (ROS) and phosphorylation of mitogen-activated protein kinases (MPKs) related genes expression during the initial phase of invasion by B. cinerea . In addition, we also found that rutin might activate plant immunity by eliciting ethylene (ET) and jasmonic acid (JA)-mediated pathways. Therefore, plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold. Graphical Abstract
研究领域:
植物病理学
·
分子生物学
✓ 已验证
DOI
Horticultural Plant Journal
·
2024
·
IF 5.7
Xuelian Zheng,Hongfei Yang,Zhichao Li,Canyu Zhou,Xinlin Chen,Kaixin Wang,Ping Yang,Guanghui Wang,Jie Zhou
Autophagy is a universal cellular process in eukaryotes that plays a critical role in plant growth and stress response. However, the role of autophagy in fruit ripening is largely unknown. Here, we demonstrated that most autophagy-related genes ( ATGs ) were up-regulated during tomato ( Solanum lycopersicum L.) fruit ripening. By using mutants of different autophagy pathway genes ( ATG6 , ATG10 , ATG18a ), we revealed that the deficiency of autophagy delayed the ripening of fruit. Compared with wild-type (WT), the production of ethylene was significantly reduced and the accumulation of lycopene was delayed in atg mutants during fruit ripening. We also observed the contents of glucose and fructose were both significantly decreased in atg mutants compared with WT, while the content of organic acids showed the opposite trend. Additionally, the negative regulator of ethylene production, APETALA2a (AP2a), interacted with ATG8 through a specific ATG8-interacting motif (AIM) and could be degraded through the autophagy pathway. These results demonstrate that autophagy plays a critical role in fruit ripening by regulating ethylene production and the accumulation of pigments, sugars and organic acids in tomato.
研究领域:
植物生物学
·
分子生物学
·
园艺学
✓ 已验证
产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2022
·
IF 6.751
Jiali Yang,Tengfei Wang,Jianbing Di,Yaping Liu,Yu Wang
Recent progress showed the potential of glutamate to regulate stress responses by affecting phytohormones homeostasis , but much remains to be explored. To decipher the role of ethylene in glutamate-induced resistance to Alternaria alternata , ethephon application, ethylene perception inhibitor (1-MCP) experiment and ethylene measurement were pursued. Concurrently, temporal alteration in ethylene biosynthesis and signaling pathways were transcriptionally analyzed in tomato fruit. Our data exhibited that ethylene conferred tomato fruit susceptibility rather than resistance to Alternaria alternata . The enhanced resistance in glutamate treatment was related to decreased ethylene production during the early stages of interaction, accompanied by lower expression levels of SlACS1 , SlACS2 and SlACO1 relative to the control. Interestingly, 1-MCP induced amounts of resistance similar to that observed in glutamate treatment, while ethylene receptors ( SlETR3 and SlETR4 ), mitogen-activated protein kinase SlMPK3 and ethylene response factor SlERF1 were down-regulated by glutamate during infection. Collectively, antagonizing the ethylene biosynthesis or signaling might play a pivotal role in glutamate-induced resistance to the necrotrophic pathogen Alternaria alternata .
研究领域:
植物学
·
植物病理学
·
分子生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yuanhao Zheng,Xuan Luo,Yuan Gao,Zhizhong Sun,Kang Huang,Weilu Gao,Huirong Xu,Lijuan Xie
Lycopene, a biologically active phytochemical with health benefits, is a key quality indicator for cherry tomatoes. While ultraviolet/visible/near-infrared (UV/Vis/NIR) spectroscopy holds promise for large-scale online lycopene detection, capturing its characteristic signals is challenging due to the low lycopene concentration in cherry tomatoes. This study improved the prediction accuracy of lycopene by supplementing spectral data with image information through spectral feature enhancement and spectra-image fusion. The feasibility of using UV/Vis/NIR spectra and image features to predict lycopene content was validated. By enhancing spectral bands corresponding to colors correlated with lycopene, the performance of the spectral model was improved. Additionally, direct spectra-image fusion further enhanced the prediction accuracy, achieving R P 2 , RMSEP, and RPD as 0.95, 8.96 mg/kg, and 4.25, respectively. Overall, this research offers valuable insights into supplementing spectral data with image information to improve the accuracy of non-destructive lycopene detection, providing practical implications for online fruit quality prediction.
研究领域:
农业工程
·
食品科学
·
光谱学
·
机器学习
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2020
·Vol. 11
·期 12
·6555-6563
·
IF 2.851
Hou Rongrong,Shi Jie,Ma Xiubing,Wei Huanran,Hu Jiajun,Tsang Yiu Fai,Gao Min-Tian
Abstract Phenolic compounds are widely used in different research fields, such as pesticides, medicines, and food. In this study, phenolic acids (PAs) were extracted from rice straw and were found to exhibit a strong inhibitory effect on Botrytis cinerea . B. cinerea mycelial growth and spore generation decreased by 86.18% and 69.10%, respectively, following 0.2 g/L phenolic acid treatment. Confocal microscopic images demonstrated that phenolic acids changed the morphology of B. cinerea . The addition of phenolic acids to B. cinerea- infected tomato leaves increased PAL (phenylalaninammo-nialyase) and PPO (polyphenol oxidase) activities, and decreased POD (peroxidases) and CAT (catalase) activities in the leaves, indicating that phenolic acids enhanced the tolerance of tomato leaves to B. cinerea by reducing oxidative stress. Chlorophyll fluorescence imaging revealed that phenolic acids could alleviate the destruction of the photosynthetic system of B. cinerea -infected leaves. These results provide new insight into the use of phenolic acids from rice straw, through which a complete green cycle of ecological production can be established. Graphic Abstract
研究领域:
植物病理学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Linling Li,Wenxin Guo,Lu Wang,Shuiyuan Cheng,Hua Cheng
Chitosan (CTS) exhibits notable moisturizing and film-forming capabilities, whereas nano selenium (SeNPs) demonstrates antioxidant and antibacterial properties. In the present study, a CTS/Se film-forming agent was prepared by dissolving CTS, glycerol, and SeNPs in 1% (v/v) glacial acetic acid through heating and ultrasonic treatment. The results revealed that SeNPs fused with CTS via intermolecular forces, thereby enhancing the tensile strength of the CTS film. The water contact angle of the CTS film increased with an increase in SeNPs concentration. The thermal stability and water retention properties of CTS/Se were superior than CTS. In a storage experiment, the content of soluble solids in tomatoes coated with CTS/Se was higher than that in tomatoes coated with CTS and the control. The CTS/Se coating inhibited gas exchange both inside and outside the film, leading to increased activities of antioxidant enzymes. This reduced the oxidation of vitamin C in the tomatoes and decreased the content of malondialdehyde. The CTS/Se film inhibited the growth of bacteria on the tomato surface and mitigated the decline of aldehyde, alcohol and ketone aroma compounds. Consequently, the CTS/Se coating alleviated the softening, aging and rotting of tomatoes. Feeding experiments conducted on mice verified the food safety of the CTS/Se.
研究领域:
食品科学
·
植物病理学
·
材料科学
✓ 已验证
DOI
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 8
·1243
·
IF 4.1
Xiao Wang,Qian Tan,Xiyue Bao,Xinyue Gong,Lingmin Zhao,Jieyin Chen,Lei Liu,Ran Li
Tomatoes are important horticultural crops worldwide. Verticillium wilt is a disease caused byVerticillium dahliaethat causes serious tomato yield losses.V. dahliaecan be classified into three distinct races in tomatoes. We identified the specificVdR3egene ofV. dahliaerace 3 and found that VdR3e triggered immune responses in the resistant tomato cultivar IVF6384. We confirmed that VdR3e triggers immune responses in the parents of IVF6384 plants and conducted transcriptome sequencing between male and female IVF6384 plants after VdR3e infiltration to analyze the potential regulatory network response to VdR3e. We found that both parents had a series of detoxification and stress resistance responses to VdR3e, but those of the male IVF6384 parent were concentrated in disease resistance-related signaling pathways. Moreover, several vital differentially expressed genes involved in functional annotation related to plant–pathogen interactions and plant hormone signaling stimulated immune responses inNicotiana benthamiana. This study provides a new and comprehensive perspective on tomato resistance to Verticillium wilt.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2025
·Vol. 13
·期 5
·406
·
IF 4.1
Huabin Liu,Chuangyang Bai,Jiahui Cai,Yue Wu,Changwei Zhu
The phytotoxicity of aluminum (Al) to plants is well known. Auxin accumulation and reactive oxygen species (ROS) burst induced by Al toxicity are the key factors in root growth inhibition. Yucasin, an auxin synthesis inhibitor, effectively ameliorates Al phytotoxicity in tomato seedlings. However, the physiological mechanisms by which yucasin alleviates Al phytotoxicity in tomatoes remain elusive. Here, we examined the regulatory mechanisms of yucasin involved in tomato seedling growth under Al conditions through phenotypic, plant physiology analysis, and cellular experiments. Exogenous indole-3-acetic acid (IAA) application increased Al accumulation in tomato seedling roots, while yucasin decreased Al accumulation. Yucasin application reduced Al-induced ROS accumulation, lipid peroxidation, and cell death, enhanced root viability, and promoted tomato seedling root growth. Further, yucasin enhanced the antioxidant enzyme activities of superoxide dismutase, catalase, and peroxidase in plants under Al conditions. The results suggest that yucasin improves the scavenging capacity of ROS by maintaining the activities of antioxidative enzymes. This study elucidates the physiological mechanism by which yucasin alleviates Al phytotoxicity, highlighting its potential to enhance plant tolerance under acidic Al conditions.
研究领域:
植物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CROP PROTECTION
·
2015
·
IF 1.493
Yuan-Xing Sun,Tong-Xian Liu
The whitefly Bemisia tabaci (biotype B) is a worldwide pest of vegetables and field crops . We tested the efficacy of imidacloprid (IM) with a root fertilizer ‘Root Feed (RF)’ (9% N, 7% Ca, 1.5% Mg and 0.1% B) sub-irrigated in the growing medium against the whitefly on tomato. Tomato seedlings (3–4 true leaves) were treated with 0, 3, 6 or 12 mg active ingredient (a.i.) seedling −1 of IM and with RF (0, 0.02, 0.04 or 0.08 ml seedling −1 ). The efficacy of 12 mg IM seedling −1 was further evaluated in the greenhouse for 60 days. The survival of B. tabaci adults, nymphs, and egg production were negatively affected by the treatments in a dosage-dependent manner. Treatment of 12 mg IM seedling −1 caused >60% adult and nymph mortality 50 days after treatment (DAT). The active ingredient of IM in tomato leaves also increased with the increase of IM dosage. The RF exhibited a limited effect on B. tabaci . However, the mortality of whitefly adults and nymphs treated with RF (0.02 or 0.04 ml seedling −1 ) positively interacted with IM, and was greater than IM alone. In the greenhouse, 12 mg IM seedling −1 greatly reduced the number of whitefly adults and increased the dry weight of the tomato plants at 30 DAT. In conclusion, application of 12 mg IM seedling −1 on tomato seedlings before transplanting effectively controled B. tabaci for up to 50 days, and the efficacy of IM combined with 0.02 ml RF seedling −1 performed even better. This could be a cost-effective method for managing B. tabaci on tomato and other vegetables.
研究领域:
农业昆虫学
·
植物保护
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xuehua Wang,Fang Chen,Lingjun Ma,Xiaojun Liao,Xiaosong Hu
High hydrostatic pressure (HHP) processing has become a commercial success in fruit and vegetable processing. Herein, the effects of HHP and high-temperature short-time (HTST) processing on metabolic profiling in tomato juice was evaluated by UPLC–MS/MS, HPLC, and GC–MS; a total of 425 metabolites, 14 carotenoids, and 56 volatile compounds were identified in tomato juice. HHP processing affects the composition of the juice less than HTST processing, considering 4 and 33 differential metabolites discriminated after HHP and HTST processing, respectively. The total lycopene and carotenoid contents in tomato juice increased after HHP processing, while the β-carotene and lycopene contents decreased after HTST processing. Further, more volatile compounds and higher contents of aldehydes that contribute to green aroma and lower contents of alcohols were observed after HHP and HTST processing, respectively. These findings provide a comprehensive understanding of the advantages of HHP processing on metabolite profiles in tomato juice.
研究领域:
食品科学
·
分析化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2016
·
IF 2.618
Xiaoya Tao,Linchun Mao,Jiayin Li,Jingxin Chen,Wenjing Lu,Si Huang
The development of wound periderm is vital to prevent water vapor loss and pathogen invasion in wounded fruit. The effect of abscisic acid (ABA) on wound-healing in harvested tomato fruit was evaluated in this study. Wounded tomato fruit were treated with ABA or fluridone (FLD, an inhibitor of ABA biosynthesis), respectively, and allowed to wound-heal at 20 °C in darkness for 4 days. Weight loss of tomato fruit reflected the intuitionistic effects of different treatments during wound-healing. Autofluorescence and histochemical staining demonstrated the development of suberized wound periderm in harvested tomato fruit at 3–4 day after wounding. Alkanoic acids, n -alkanes, unsaturated fatty acids and pentacosane represented the major components in the suberin. ABA-treated fruit showed enhanced autofluorescence, histochemical intensity and suberin deposition along with increased activities of phenylalanine ammonia-lyase (PAL) and peroxidase (POD). FLD treatment, however, alleviated the features of suberization and enzyme activities during wound-healing. The results suggest that ABA is involved in the stimulation of wound-induced suberization in harvested tomato fruit.
研究领域:
植物学
·
园艺学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）