BIOSENSORS & BIOELECTRONICS
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2021
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IF 10.618
Hongmei Li,Qing Wang,Jun Tang,Ningning Gao,Xiaxia Yue,FuRu Zhong,Xiaoyi Lv,Jihong Fu,Tao Wang,Cailing Ma
As a rapid and non-destructive biological serum detection method, SERS technology was widely used in the screening and medical diagnosis of various diseases by combining the analysis of serum SERS spectrum and multivariate statistical algorithm. Because of the high complexity of serum components and the variability of SERS spectra, which often resulted in the phenomenon that the SERS spectrum of the same biological serum was significantly different due to the different test conditions. In this experiment, through the dilution treatment of the serum and the systematic test of the serum of all concentration gradients with lasers of wavelength of 785, 633 and 532 nm, the most suitable conditions for detecting the serum were investigated. The experimental results showed that only when the serum is diluted to low concentration (10 ppm), the SERS spectrum with high reproducibility and stability could be obtained, furthermore, the low concentration serum had weak tolerance to laser, and 532 nm laser was not suitable for serum detection. In this paper, a set of test scheme for obtaining highly stable serum SERS spectra was established by using high-performance gold nanoparticles (Au NPs) as the active substrate of SERS. Through comparative analysis of SERS spectrum of serum of normal people and cervical cancer, the reliability of the established low-concentration serum test program was verified, as well as its great potential advantages in disease screening and diagnosis.
研究领域:
分析化学
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生物医学
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光谱学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2023
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IF 12.6
Yu Li,Miaomiao Cai,Wenwen Zhang,Ying Liu,Xiaoqing Yuan,Na Han,Jing Li,Shengnan Jin,Chunming Ding
The CRISPR-Cas12a RNA-guided complexes hold immense promise for nucleic acid detection. However, limitations arise from their specificity in detecting off-targets and the stability of the signal molecules. Here, we have developed a platform that integrates multiplex amplification and nanomolecular-reporting signals, allowing us to detect various clinically relevant nucleic acid targets with enhanced stability, sensitivity, and visual interpretation. Through the electrostatic co-assembly of the Oligo reporter with oppositely charged nanoparticles, we observed a significant enhancement in its stability in low-pollution environments, reaching up to a threefold increase compared to the original version. Additionally, the fluorescence efficiency was expanded by three orders of magnitude, broadening the detection range considerably. Utilizing a multiplex strategy, this assay can accomplish simultaneous detection of multiple targets and single-point indication detection of nine specific targets. This significant advancement heightened the sensitivity of disease screening and improved the accuracy of diagnosing disease-related changes. We tested this assay in a colorectal cancer model, demonstrating that it can identify DNA methylation features at the aM-level within 40–60 min. Validation using clinical samples yielded consistent results with qPCR and bisulfite sequencing, affirming the assay's reliability and potential for clinical applications.
研究领域:
生物技术
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Chiyu Ma,Yong Qiu,Tao Liang,Nan Jiang,Liubing Kong,Jianguo Wu,Xianyou Sun,Guangqing Ren,Xinwei Wei,Ping Wang,Hao Wan
Background Ionic calcium (Ca 2+ ) plays a crucial role in maintaining normal physiological and biochemical functions within the human body. Detecting the concentration of Ca 2+ is of utmost significance for various purposes, including disease screening, cellular metabolism research, and evaluating drug effectiveness . However, current detection approaches such as fluorescence and colorimetry face limitations due to complex labeling techniques and the inability to track changes in Ca 2+ concentration. In recent years, extensive research has been conducted in this field to explore label-free and efficient approaches. Results In this study, a novel light-addressed potentiometric sensor (LAPS) using silicon-on-sapphire technology , has been successfully developed for Ca 2+ sensing. The Ca 2+ -sensitive LAPS achieved a wide-range detection of Ca 2+ , ranging from 10 −2 M to 10 −7 M, with an impressive detection limit of 100 nM. These advancements are attributed to the ultra-thin silicon layer, silicon dioxide layer, and solid-state silicon rubber sensitive membrane around 6 μm. Furthermore, the sensor demonstrated the ability to dynamically monitor fluctuations in Ca 2+ concentration ranging from 10 −9 M to 10 −2 M within a solution. Its remarkable selectivity , specificity, and long-term stability have facilitated its successful application in the detection of Ca 2+ in human serum and urine. Significance and novelty This work presents a Ca 2+ -sensitive sensor that combines a low detection limit and a wide detection range. The development represents the emergence of a label-free and rapid Ca 2+ detection tool with immense prospects in home-based health monitoring, community disease screening, as well as cellular metabolism, and drug screening evaluations.
研究领域:
生物医学工程
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分析化学
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传感器与执行器
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医学诊断
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PubMed
高影响因子
Nature Communications
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2022
·Vol. 13
·期 1
·1-16
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IF 17.694
Yang Bin,Kong Jilie,Fang Xueen
Recent advances in biointerfaces have led to the development of wearable devices that can provide insights into personal health. As wearable modules, microneedles can extract analytes of interest from interstitial fluid in a minimally invasive fashion. However, some microneedles are limited by their ability to perform highly effective extraction and real-time monitoring for macromolecule biomarkers simultaneously. Here we show the synergetic effect of CRISPR-activated graphene biointerfaces, and report an on-line wearable microneedle patch for extraction and in vivo long-term monitoring of universal cell-free DNA. In this study, this wearable system enables real-time monitoring of Epstein-Barr virus, sepsis, and kidney transplantation cell-free DNA, with anti-interference ability of 60% fetal bovine serum, and has satisfactory stable sensitivity for 10 days in vivo. The experimental results of immunodeficient mouse models shows the feasibility and practicability of this proposed method. This wearable patch holds great promise for long-term in vivo monitoring of cell-free DNA and could potentially be used for early disease screening and prognosis.
研究领域:
生物技术
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医疗器械
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分子生物学
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可穿戴技术
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基因组学
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DOI
PubMed
高影响因子
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