Journal of Analytical Science and Technology
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2024
·Vol. 15
·期 1
·1-8
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IF 2.5
Li Yunxiao,Wang Qiuxia,Wang Yali
The abnormally expressed microRNAs (miRNAs) serve as crucial indicators for disease diagnosis and are strongly associated with the progression of various diseases. The quantification of miRNAs is highly significant for the clinical diagnosis and treatment of various types of cancers. This study utilizes the CRISPR/Cas13a system, which combines CRISPR RNA (crRNA) and CRISPR-associated Cas13a, to recognize the miRNA directly and specifically, thus activating the trans-cleavage activity of Cas13a. By integrating the CRISPR-Cas13a system with an exonuclease-iii (Exo-iii)-assisted chain cleavage of silver ions (Ag+)-aptamer and an Ag+ -based color reaction, a method for the detection of miRNA that is specific and sensitive is developed. This approach demonstrated exceptional sensitivity in detecting miRNA, with a low detection limit of 5.12 fM. Moreover, the suggested method was effectively utilized to precisely measure the amount of miRNA in intricate biological samples, demonstrating its practical applicability. Furthermore, due to its elevated sensitivity and simple probe design and fluorophore labeling, the suggested colorimetric technique holds great potential for use in clinical diagnostics.
研究领域:
分子生物学
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医学诊断
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生物技术
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DOI
高影响因子
ANALYTICAL LETTERS
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2024
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IF 1.6
Jianming Zhang,Yancheng Jiang,Yubin Wang,Zhishan Zhang
MicroRNAs (miRNAs) are endogenous, noncoding, and sequence-specific single-stranded RNA regulatory factors whose abnormal expression levels are closely related to various diseases, especially cancer, and are considered effective biomarkers for disease diagnosis and prognosis. Since the concentrations of miRNAs in the body are low and disease diagnosis by detecting single miRNAs may result in a high false positive, it is essential to construct a sensitive biosensor for the determination of multiple miRNAs. In this work, an enzyme-free signal amplification assay based on plasma-enhanced fluorescence (PEF) is reported for the one-step simultaneous determination of two miRNAs. Gold nanorods (AuNRs) were functionalized with fluorescein isothiocyanate (FAM)-tagged hairpin DNA1 (H1) and cyanine 5.5 (Cy5.5)-tagged hairpin DNA2 (H2). The fluorescence signals of FAM and Cy5.5 were quenched due to their proximity to the surface of the AuNRs. In the presence of target miRNA-21 and miRNA-155, H1 and H2 complemented miRNA-21 and miRNA-155, respectively, and the fluorescence signals of FAM and Cy5.5 were not only restored but amplified due to the plasma-enhanced fluorescence. A suitable linear range (0.1 to 10 nM) and low limits of detection for miRNA-21 (23 pM) and miRNA-155 (1.6 pM) were achieved. The developed assay for the one-step simultaneous measurement of two miRNAs is simple, fast and sensitive, exhibiting great potential for monitoring these biomarkers.
研究领域:
生物化学
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分子生物学
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医学诊断
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Yuting Li,Yunqing Tang,Lu Qian,Yanqin Zhang,Yuewen Zhai,Jinnan Zhang,Yu Li,Yanfang Deng,Juequan Li,Yuhang Li,Bing Xue,Yunlong Liu,Siwen Li
RNA in small extracellular vesicles (sEVs) from plasma is a reliable biomarker for disease diagnosis. However, the cumbersome steps and operator dependency for extraction and analysis of sEVs RNA in plasma have limited their application. Here, we developed a multisatellites@planets molecular imprinting detection platform for sEVs capture and RNA analyzation in a one-step process directly from a small amount of plasma. In this platform, magnetic beads as “planet” templates were utilized for size recognition of sEVs, and Au nanoparticles as “multi-satellites” templates were used to immobilize liposome probes for sEVs grasp and RNA detection through membrane fusion. As for the detection accuracy, the results from our platform demonstrate a high level of concordance with qRT-PCR (R2 value of 0.9937). Moreover, we successfully used 10 μL of plasma to analyze sEVs RNA, effectively differentiating healthy donors and lung cancer patients with an accuracy of 91.18% (combined MALAT1 and miR-21). This novel platform provides a pretreatment-free, user-friendly, highly accurate, and sensitive strategy for detecting the RNA of sEVs in plasma, indicating its potential capability for disease diagnosis.
研究领域:
生物学
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医学
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生物技术
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Yuqiang Hu,Zhen Zhang,Weicong Ye,Wei Zhang,Minghao Hu,Wenqian Yuan,Hongbo Wang,Xianjin Xiao,Tongbo Wu
Human apurinic/apyrimidinic endonuclease 1 (APE1) plays a crucial role in DNA repair and gene regulation. The abnormal variations of the concentration of APE1 in the human blood and tissue cells are highly correlated to various diseases. Thus, APE1 can be used as a biomarker to aid clinical diagnosis of diseases. Detection of APE1 activity with high sensitivity and selectivity simultaneously is difficult to achieve. Here we provide a DNA structure-mediated fluorescent biosensor to solve the above problem. Upon the existence of APE1, the apurinic/apyrimidinic site will be cleaved, and the terminal structure of the hairpin DNA substrate will change. Then the terminal deoxynucleotidyl transferase and endonuclease IV could provide dual fluorescent signal amplification. Through the ingenious structure design of the biosensor, we have improved the reactivity of APE1 and minimized the interference of other enzymes. The results indicate that the detection limit for APE1 is as low as 1.7 × 10 −6 U/mL. The biosensor has been successfully applied to the detection of APE1 in real biological samples and the screening of APE1 inhibitors.
研究领域:
生物化学
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分子生物学
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医学诊断
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DOI
高影响因子
Nature Machine Intelligence
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2025
·Vol. 7
·期 5
·743-757
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IF 23.9
Xue Bingsen,Bi Xinyuan,Dong Zheyi,Xu Yunzhe,Liang Minghui,Fang Xin,Yuan Yizhe,Wang Ruoxi,Liu Shuyu,Jiao Rushi,Chen Yuze,Zu Weitao,Wang Chengxiang,Zhang Jianhao,Liu Jiang,Zhang Qin,Yuan Ye,Xu Midie,Zhang Ya,Wang Yanfeng,Ye Jian,Jin Cheng
Analysing metabolites in bioliquids through various spectroscopic methods provides valuable insights into the metabolic phenotypes. Deciphering spectral data has greatly benefited from deep-learning methods; however, data-driven solutions often struggle with data dependence on different devices, samples and spectral modalities. Most current task-specific methods have limited generalizability to different spectral analysis problems, including preprocessing, quantification and interpretation. Here, we developed a pretrained foundation model, termed deep-spectral component filtering (DSCF) through a self-supervised approach termed spectral component resolvable learning. By acquiring general spectral knowledge, DSCF achieved state-of-the-art performance for five distinct spectral analysis tasks on 11 datasets. Notably, the general pretraining led to zero-shot spectral denoising and trace-level quantification in complex mixtures. DSCF achieved molecule-level interpretation of surface-enhanced Raman spectra and mapped serum metabolic profiles from nearly 600 individuals for various diseases, including stroke, Alzheimer’s disease and prostate cancer. Overall, the proposed foundation model illustrates promising generalizability for spectral analysis and offers a clear and feasible pathway for general spectral analysis.
研究领域:
生物信息学
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医学
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化学计量学
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人工智能
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DOI
高影响因子
AIP Advances
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2023
·Vol. 13
·期 3
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IF 1.6
Wang Qiaolin,Xu Lihe,Qin Zhengbo,Yang Xinyan,Zheng Xianfeng
Deoxyribonucleic acid (DNA) carries the genetic information necessary for the synthesis of RNA and proteins; it is a biological macromolecule essential for the development and proper functioning of living organisms and is composed of nucleobases, deoxyribose, and phosphate. The four nucleobases in DNA are adenine (AD), guanine (GU), thymine (TY), and cytosine (CY). Abnormal concentrations of these four nucleobases in an organism have a significant impact on disease diagnosis. Therefore, the qualitative and quantitative detection of these DNA nucleobases in organisms is helpful to diagnose certain diseases. In this work, we report the simultaneous determination of purine (AD, GU) and pyrimidine (TY, CY) nucleobases in DNA using laser-induced acoustic desorption (LIAD) with electron ionization (EI)/time-of-flight mass spectrometry (TOFMS). The purine (MW 120 Da) samples were used as model compounds to assess the sensitivity and quantitative performance of the instrument. Its limits of detection assessed using the LIAD/EI/MS method were ∼0.5–1.2 pg under optimal conditions, and their calibration curves exhibited good linearity (R 2 = 0.98). The LIAD/TOFMS was successfully applied in the simultaneous detection of AD, GU, TY, and CY in real DNA samples. The advantage of this technique is simple, fast, and without complex pre-treatment processes. In addition, a quartz-enhanced LIAD (QE-LIAD) source was used to improve the signal strength. The desorption for complex biomolecules shows that the QE-LIAD is still a “gentle” desorption source.
研究领域:
分析化学
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分子生物学
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医学诊断
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Yujie Duan,Shanshan Yu,Siyuan Xia,Lei Wang,Zihan Zhao,Hui Liu,Huanhuan Huang,Hongxiang Zhu,Li Wang,Hui He
The distinction of high reactive oxygen species (hROS) types has positive significance for disease diagnosis. Here, to visibly assess hROS types and levels, a cellulosic multi-emission fluorescence probe was designed by incorporating proline-protected gold nanoclusters (Em: 450 nm), glutathione-protected gold nanoclusters (Em: 680 nm) and o-phenylenediamine (Em: 565 nm) onto cellulose nanocrystal. Ingeniously, the probe displayed the stabilized, quenched and activated fluorescence signals corresponding to hROS at 450 nm, 680 nm, and 565 nm, respectively. The response time of the probe for hROS was greatly reduced from 29 min to 7 min through gold nanoclusters releasing Au(Ⅲ) during the fluorescence quenching process to accelerate the fluorescence opening of o-phenylenediamine. Therefore, the probe with long-term stability (12 months) possessed excellent sensitivity and selectivity to distinguish hROS types, and exhibited visually yellow, white, and green fluorescence for •OH, ClO − , and ONOO − , respectively, and the corresponding detection limits were 0.56 μM, 0.73 μM and 0.81 μM within 5 min, 6 min and 7 min, respectively. The design strategy provides the ideas for constructing multi-emission probe to distinguish multiple analytes at similar levels.
研究领域:
生物化学
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纳米医学
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分析化学
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DOI
高影响因子
Nature Communications
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2024
·Vol. 15
·期 1
·1-8
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IF 14.7
Xing Xing,Wu Luyan,Zhang Yuchen,Pan Jiahao,Ishigaki Yusuke,Xie Huaqing,Suzuki Takanori,Ye Deju,Zhang Jianhua,Zhang Weihua,Lu Zhenda
Early disease diagnosis hinges on the sensitive detection of signaling molecules. Among these, hydrogen sulfide (H 2 S) has emerged as a critical player in cardiovascular and nervous system signaling. On-chip immunoassays, particularly nanoarray-based interfacial detection, offer promising avenues for ultra-sensitive analysis due to their confined reaction volumes and precise signal localization. Beyond the DNA or protein biomolecules array, this work presents a promising hybrid small molecule nano-array for H 2 S detection, using the power of dual molecules: a dye for fluorescence emission and a quencher with specific H 2 S reactivity. Upon H 2 S interaction, the quenched fluorescence reignites, creating an easily detectable array of bright spots. The molecule nano-array sensor shows exceptional responses to H 2 S over 8 magnitudes of dynamic range from 1 fM to 0.1 μM, with a remarkable detection limit of 1 fM, just using a 10 μL solution. This H 2 S detection method has the potential to significantly improve bioassay platforms, and the hybrid small-molecule nano-arrays we developed could be a valuable tool for advancing signaling molecule detection.
研究领域:
生物化学
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医学检测
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
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2025
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IF 2.6
Yu-Lan Zhang,Cheng-Xiao Deng,Jian-Mei Zou,Jia-Le Hu,Wei Li,Jin-Fang Nie,Yun Zhang
Biothiol analysis holds critical importance in disease diagnosis, but the highly similar structures of biothiols pose a major obstacle to their practical detection. Herein, a straightforward yet efficient CoOOH nanoflake (CoOOH NF)-triggered dual-color fluorescence sensor array has been developed for the discrimination of biothiols. Owing to their high oxidase-like activity, CoOOH NFs efficiently catalyze the oxidation of fluorometric signal indicators, generating diverse fluorescence signals. However, the presence of biothiols induced structural degradation of CoOOH NFs via redox etching, thereby inhibiting the fluorescence reactions. Based on the differential reducing capacities of biothiols, the sensor array produced unique fluorescence response patterns, which were further analyzed using principal component analysis (PCA) and hierarchical cluster analysis (HCA). The results demonstrated that the sensor array could reliably discriminate three biothiols (glutathione (GSH), cysteine (Cys), and homocysteine (Hcy)) across a broad concentration range (0.5–100 μM), as well as their mixtures with varying molar ratios. Importantly, this strategy was successfully applied to distinguish cancer cells from normal cells and to analyze clinical serum samples, highlighting its potential for medical diagnostics.
研究领域:
分析化学
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生物传感
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医学诊断
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纳米材料
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2015
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IF 6.409
Qi-Yong Cai,Jie Li,Jia Ge,Lin Zhang,Ya-Lei Hu,Zhao-Hui Li,Ling-Bo Qu
Glutathione (GSH) serves many cellular functions and plays crucial roles in human pathologies. Simple and sensitive sensors capable of detecting GSH would be useful tools to understand the mechanism of diseases. In this work, a rapid fluorescence “switch-on” assay was developed to detect trace amount of GSH based on carbon dots–MnO 2 nanocomposites , which was fabricated through in situ synthesis of MnO 2 nanosheets in carbon dots colloid solution. Due to the formation of carbon dots–MnO 2 nanocomposites , fluorescence of carbon dots could be quenched efficiently by MnO 2 nanosheeets through fluorescence resonance energy transfer (FRET). However, the presence of GSH would reduce MnO 2 nanosheets to Mn 2+ ions and subsequently release carbon dots, which resulted in sufficient recovery of fluorescent signal. This proposed assay demonstrated highly selectivity toward GSH with a detection limit of 300 nM. Moreover, this method has also shown sensitive responses to GSH in human serum samples, which indicated its great potential to be used in disease diagnosis. As no requirement of any further functionalization of these as-prepared nanomaterials , this sensing system shows remarkable advantages including very fast and simple, cost-effective as well as environmental-friendly, which suggest that this new strategy could serve as an efficient tool for analyzing GSH level in biosamples.
研究领域:
生物化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Haiqing Wang,Wei Shi,Lei Hou,Chunhui Li,Zhiquan Wang,Lei Yang,Juncheng Cao
The rapid and accurate identification of the types and contents of early pathological markers by THz technology are of particular importance for the prevention and treatment of major diseases. Nevertheless, these markers usually contain interference from water and other non-target molecules, resulting in low signal-to-noise ratio (SNR) and making identification and quantitative analysis difficult. Here, based on THz spectroscopy from a high-power THz source radiated by LiNbO 3 , we perform quantitative and real-time THz detection of mixtures (α-lactose monohydrate and 4-aminobenzoic acid) in liquids. The results demonstrate that the absorption spectra of the aqueous biomolecular mixtures exhibit an accumulation of THz features of each pure product, i.e., the amplitude of the absorption peaks is proportional to the mixing ratio, while the corresponding absorption baseline increases with decreasing concentration. Furthermore, the content of the target substance can be calculated from the linear relationship between the absorption spectra of pure and mixed samples. This technology will support the future application of THz-TDS in early disease diagnosis under complex states and environments.
研究领域:
生物医学工程
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光谱学
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医学检测
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产品关联
DOI
PubMed
高影响因子
Journal of Analytical Science and Technology
·
2025
·Vol. 16
·期 1
·1-7
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IF 2.8
Zhang Zhe,Xie Qiaoyi,Zhu Chenbo,Shao Huali,Wu Boying
Alterations in microRNA (miRNA) expression profiles play a pivotal role in the initiation and progression of various diseases, including pediatric pneumonia. Consequently, the development of sensitive, specific, and precise methodologies for miRNA detection represents a promising tool for clinical diagnosis and therapeutic intervention. This study proposes a highly sensitive miRNA detection approach utilizing catalytic hairpin assembly (CHA)-mediated probe release through chain extension and cascade signal amplification. The method enables precise discrimination of target miRNAs from other RNA species via a dual-target recognition mechanism. Furthermore, the incorporation of four signal amplification cycles confers substantial amplification efficiency, permitting accurate detection of low-abundance miRNAs. This system employs cascade isothermal amplification, demonstrating a linear response across concentrations from 1 fM to 100 pM and achieving a detection limit of 0.54 fM. The methodology exhibits significant potential for practical application in complex biological samples, providing a novel platform for profiling miRNA expression patterns and elucidating their mechanistic roles in disease pathogenesis.
研究领域:
分子诊断学
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RNA生物学
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生物医学工程
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临床检验医学
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DOI
高影响因子
危险品化学品经营许可证（带存储）