CHINESE JOURNAL OF CHEMISTRY
·
2021
·Vol. 40
·期 8
·946-952
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IF 6
Hailan Zhong,Hao Wu,Shuoran Chen,Liwei Cao,Changqing Ye,Fan Xia,Fengyu Li
Comprehensive Summary Aiming to the enormous requirement for the epidemic defense researches, we designed and constructed a spherical colloidal virus particle (CVP) to mimic nature virus in morphology, physical, chemical and biological characteristics, via coating spiky protein on colloidal nanoparticles (CNPs) core with bulge hierarchical nanomorphology. The novel virus-like surface nanoparticles can easily be synthesized. The physical, chemical nature and the formation mechanism of the prepared CVPs were characterized and discussed. The synthesized CVPs are similar in size and envelope thickness to common natural viruses. It was demonstrated that the diameter of CVPs is about 238 ± 12 nm, including an 8 nm thickness protein crown with bulges of 33 nm in average width. The CVPs with an isoelectric point of 4.5, meets the native virus property of negative charge under neutral condition. The protein crown enhances the roughness remarkably from 10 nm (CNPs) to 22 nm (CVPs) determined by atomic force microscopy. Thanks to the biomimetic rough morphology, the CVPs show greatly superior cellular uptake performance compared to CNPs, ovalbumin (OVA) and smoothed colloidal particles (SCPs). The formation mechanism of protein crown with specific thickness can be attributed to the electrostatic interaction, protein's flexible structure and specific wettability. These results indicate that the as-prepared artificial virions mimic nature viruses in multi-dimension, in terms of size, surface rough morphology, surface negative charge and glycoprotein envelope composition. The synthetic colloidal virus particles pave a facile way toward engineering virus particles substitute for virus-related diseases prevention, diagnostics and cellular delivery vectors.
研究领域:
生物材料学
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病毒学
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纳米医学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Wenwei Pan,Rui You,Shuaihua Zhang,Ye Chang,Feng Zhou,Quanning Li,Xuejiao Chen,Xuexin Duan,Ziyu Han
Nanochannel-based resistive pulse sensing (nano-RPS) system is widely used for the high-sensitive measurement and characterization of nanoscale biological particles and biomolecules due to its high surface to volume ratio. However, the geometric dimensions and surface properties of nanochannel are usually fixed, which limit the detections within particular ranges or types of nanoparticles . In order to improve the flexibility of nano-RPS system, it is of great significance to develop nanochannels with tunable dimensions and surface properties. In this work, we proposed a novel multi-module self-assembly (MS) strategy which allows to shrink the geometric dimensions and tune surface properties of the nanochannels simultaneously. The MS-tuned nano-RPS device exhibits an enhanced signal-to-noise ratio (SNR) for nanoparticle detections after shrunk the geometric dimensions by MS strategy. Meanwhile, by tuning the surface charge, an enhanced resolution for viral particles detection was achieved with the MS-tuned nano-RPS devices by analyzing the variation of pulse width due the tuned surface charge. The proposed MS strategy is versatile for various types of surface materials and can be potentially applied for nanoscale surface reconfiguration in various nanofluidic devices.
研究领域:
纳米生物学
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jia Wei,Wanjun Wang,Qiong Yu,Mingrui Zhang,Fengyu Xue,Beibei Fan,Tong Zhang,Yanpeng Gao,Juan Li,Xianying Meng,Bo Pang
Mpox (formerly referred as Monkeypox) outbreak has been declared a Public Health Emergency of International Concern. However, traditional polymerase chain reaction (PCR) diagnostic technology is not ideal for on-site applications. To conduct the sample-to-result Mpox viral particles detection outside the laboratories, we developed an easy-to-operate palm-size pouch, termed Mpox At-home Self-Test and point-of-caRe Pouch (MASTR Pouch). In this MASTR Pouch, the fast and accurate visualization was achieved by incorporating recombinase polymerase amplification (RPA) with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas12a system. From viral particle lysis to naked eye readout, MASTR Pouch required only four simple steps to accomplish the analysis process within 35 min. Fifty-three Mpox pseudo-viral particles in exudate (10.6 particles/μL) were able to be detected. To verify the practicability, 104 mock Mpox clinical exudate specimens were tested. The clinical sensitivities were determined to be 91.7%− 95.8%. There was no false-positive result, validating the 100% clinical specificity. MASTR Pouch approaches the WHO's ASSURD criteria for point-of-care diagnostic, which will be beneficial for mitigating Mpox's global spread. The versatility potential of MASTR Pouch could further revolutionize infection diagnosis.
研究领域:
病毒学
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分子诊断
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公共卫生
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生物技术
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-15
·
IF 3.8
Chen Linli,Guo Xiaojing,Lin Wen,Huang Yingying,Zhuang Suling,Li Qianfeng,Xu Jianhua,Ye Shengnan
Lytic induction therapy was devised to selectively combat malignancies associated with Epstein–Barr virus (EBV) by triggering viral reactivation from latency. At present, the major challenges of lytic induction therapy are to maximize reactivating efficiencies and meanwhile minimize infectious virion production. C210, a novel curcumin derivative with potent Hsp90 inhibitory activity, was explored for EBV-reactivating and virion-producing effects in EBV-positive nasopharyngeal carcinoma (NPC) and gastric carcinoma (GC) cell lines. And the molecular mechanisms underlying these effects were determined. Follow C210 treatment, EBV lytic RNAs and proteins were upregulated, but infectious virions were not produced. Knockdown of heat shock protein 90 (Hsp90) induced expression of lytic RNAs and proteins, and diminished C210-driven EBV lytic induction. Pretreatment with an X box binding protein 1 (XBP1) inhibitor reduced C210-induced EBV lytic RNA. Furthermore, we demonstrated that C210 inhibited the binding of Hsp90 with its clients, signal transducer and activator of transcription 3 (STAT3) and xeroderma pigmentosum group B-complementing protein (XPB), which subsequently promoted their proteasomal degradation. Degradation of STAT3 by C210 enhanced the EBV-reactivating and anticancer capacity of suberoylanilide hydroxamic acid (SAHA). Depletion of XPB blocked SAHA-induced expression of late viral genes and production of infectious virions. These results elucidate a novel Hsp90 inhibitor targeting EBV lytic phase and extend the research on lytic induction strategy, which may offer reference value in the treatment of EBV-positive malignancies.
研究领域:
肿瘤学
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病毒学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2023
·
IF 6.7
Haipeng Cui,Wenwei Pan,Tiechuan Li,Xiaotian Shen,Ye Chang,Wei Pang,Xuexin Duan
Virus infections remain one of the principal causes of morbidity and mortality worldwide. The current gold standard approach for diagnosing pathogens requires access to reverse transcription–polymerase chain reaction (RT‒PCR) technology. However, separation and enrichment of the targets from complex and diluted samples remains as a major challenge. In this work, we proposed a micromotor-based sample preparation concept for the efficient separation and concentration of target viral particles before PCR. The micromotors are functionalized with antibodies with a 3D polymer linker and are capable of self-propulsion by the catalytic generation of oxygen bubbles for selective and positive virus enrichment. This strategy significantly improves the enrichment efficiency and recovery rate of virus (up to 80% at 104 tu mL-1 in a 1 mL volume within just 6 min) without external mixing equipment. The method allows the Ct value in regular PCR tests appearing 6-7 cycles earlier and a detection limit of 1 tu mL-1 for target virus from swap samples. A point-of-need test kit is designed based on the micromotors which can be readily applied to pretreat a large volume of samples.
研究领域:
生物技术
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病毒学
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2016
·
IF 2.687
Bin Chen,Qing Ye,Kangping Zhou,Yefu Wang
A novel type of aptamer-functionalized immunoadsorbent was prepared and characterized to remove HCV particles, a promising option of extracorporeal immunoadsorption (ECI) therapy against HCV. Herein, we fabricated a HCV-specific immunoadsorbent where single-stranded DNA aptamers reported and studied previously, modified with amino group at the 5′ terminus, was immobilized covalently onto surfaces of carboxylated-derivative sepharose 4FF beads through N -hydroxysuccinimide (NHS) linkage. Then the adsorbents was evaluated and characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Subsequently, we also confirmed that proposed immunoadsorbents exhibited a favorable biocompatibility as well as specificity. In addition, time-dependent effects of the eradication capacity of aptamer functionalized sepharose beads against HCV was investigated. With the optimized time point, the decontamination performance of HCV particles was assessed by real-time quantitative PCR (qPCR) followed by nucleic acid-based hybridization (NAH), which shows sorbents with an aptamer density of 2 nmol ligand/ml resin could remove approximately 80% (i.e. 8.9 × 10 6 HCV particles/ml resin) of the HCV genotype 2a cultivated in vitro and 75% (vary with the intial concentration of HCV from about 7.5 × 10 4 –4.4 × 10 5 HCV particles/ml resin) of the HCV samples from human plasma samples. All these results indicated that the novel aptamer-based adsorbents could effectively remove HCV particles and likely serve as a novel therapy option or at least supplementary for the treatment regimen of HCV.
研究领域:
病毒学
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分子生物学
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治疗学
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产品关联
DOI
PubMed
高影响因子
Nanoscale Advances
·
2023
·Vol. 5
·期 5
·1433-1449
·
IF 4.7
Liuyang Wang,Xuan Lin,Yanan Sheng,Hongyu Zhu,Zhengjun Li,Zhiguo Su,Rong Yu,Songping Zhang
Encapsulating antigens with zeolitic imidazole framework-8 (ZIF-8) exhibits many advantages in vaccine development. However, most viral antigens with complex particulate structures are sensitive to pH or ionic strength, which cannot tolerate harsh synthesis conditions of ZIF-8. Balancing the viral integrity and the growth of ZIF-8 crystals is crucial for the successful encapsulation of these environment-sensitive antigens in ZIF-8. Here, we explored the synthesis of ZIF-8 on inactivated foot and mouth disease virus (known as 146S), which is easily disassociated into no immunogenic subunits under the existing ZIF-8 synthesis conditions. Our results showed that intact 146S could be encapsulated into ZIF-8 with high embedding efficiency by lowering the pH of the 2-MIM solution to 9.0. The size and morphology of 146S@ZIF-8 could be further optimized by increasing the amount of Zn2+ or adding cetyltrimethylammonium bromide (CTAB). 146S@ZIF-8 with a uniform diameter of about 49 nm could be synthesized by adding 0.01% CTAB, which was speculated to be composed of single 146S armored with nanometer-scale ZIF-8 crystal networks. Plenty of histidine on the 146S surface forms a unique His-Zn-MIM coordination in the near vicinity of 146S particles, which greatly increases the thermostability of 146S by about 5 °C, and the nano-scale ZIF-8 crystal coating exhibited extraordinary stability to resist EDTE-treatment. More importantly, the well-controlled size and morphology enabled 146S@ZIF-8(0.01% CTAB) to facilitate antigen uptake. The immunization of 146S@ZIF-8(4×Zn2+) or 146S@ZIF-8(0.01% CTAB) significantly enhanced the specific antibody titers and promoted the differentiation of memory T cells without adding another immunopotentiator. This study reported for the first time the strategy of the synthesis of crystalline ZIF-8 on an environment-sensitive antigen and demonstrated that the nano-size and appropriate morphology of ZIF-8 are crucial to exert adjuvant effects, thus expanding the application of MOFs in vaccine delivery.
研究领域:
病毒学
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纳米技术
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免疫学
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材料科学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 20
·4511-4522
·
IF 7
Wenwei Pan,Ziyu Han,Ye Chang,Xu Yan,Feng Zhou,Sihong Shen,Xuexin Duan
Viral particles bind to receptors through multivalent protein interactions. Such high avidity interactions on sensor surfaces are less studied. In this work, three polyelectrolytes that can form biosensing surfaces with different interfacial characteristics in probe density and spatial arrangement were designed. Quartz crystal microbalance, interferometry and atomic force microscopy were used to study their surface density and binding behaviors with proteins and virus particles. A multivalent adsorption kinetic model was developed to estimate the number of bonds from the viral particles bound to the polyelectrolyte surfaces. Experimental results show that the heterogeneous 3D surface with jagged forest-like structure enhances the virus capture ability by maximizing the multivalent interactions. As a proof of concept, specific coronavirus detection was achieved in spiked swab samples. These results indicate the importance of both probe density and their spatial arrangement on the sensing performance, which could be used as a guideline for rational biosensing surface design.
研究领域:
生物传感器
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病毒学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Shijie Sun,Yancao Chen,Ruili Xu,Jinhui Shang,Huiwen Li,Bin Xiong,Xiao-Bing Zhang
To achieve reliable detection of viral antigens, it is critical to develop of antifouling sensor arrays. Herein, we introduce a robust strategy for facile design and construction of hydrogel-based antifouling sensor arrays with a light-responsive bifunctional crosslinker. Upon UV light irradiation, the capture antibodies and blocking agents can be chemically conjugated onto the hydrogel interface to generate antifouling sensor arrays with desirable protein patterns with the aid of a hole-array mask. We demonstrated the antifouling sensor arrays for the quantitative detection of SARS-CoV-2 spike protein with satisfying sensitivity and high signal-to-background ratio. We further confirmed the prepared multiplexed sensor arrays for the detection of multiple viral proteins simultaneously in complex environments. Moreover, we achieved the quantitative detection of synthetic virus-like particles and SARS-CoV-2 pseudoviruses with satisfying detection performances. All these evidences suggested that the hydrogel-based antifouling sensor arrays holds great potential for quantitative analysis of viral antigens and virus particles in real applications.
研究领域:
生物传感器
·
分析化学
·
材料科学
·
病毒学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）