JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 7
·3414-3426
·
IF 4.1
Yufeng Liu,Jinming Hu,Meng M. Li,Guangyong Zhao
BACKGROUND The objectives of this study were to investigate the effects of taurine on rumen fermentation, rumen bacterial community and metabolomics, nitrogen metabolism and plasma biochemical parameters in beef steers. Six castrated Simmental steers (liveweight 402 ± 34 kg) and three levels of taurine (0, 20, 40 g d −1 ) were assigned in a replicated 3 × 3 Latin square design. Each experimental period included 15 days for adaptation and 5 days for sampling. RESULTS Supplementing taurine did not affect the ruminal pH or concentrations of ammonia nitrogen and volatile fatty acids ( P > 0.10), but linearly increased the ruminal concentrations of taurine ( P < 0.001) and microbial crude protein ( P = 0.041). Supplementing taurine linearly increased the neutral detergent fiber digestibility ( P = 0.018), and tended to linearly increase dry matter digestibility ( P = 0.095), tended to increase the fecal nitrogen excretion ( P = 0.065) and increased the urinary taurine excretion ( P < 0.001). Supplementing taurine quadratically increased the plasma concentration of triglycerides ( P = 0.017), tended to linearly decrease growth hormone ( P = 0.074), but did not affect other plasma parameters ( P > 0.10). Supplementing taurine modified the rumen bacterial community and increased the ruminal concentration of taurine metabolite 2-hydroxyethoxysulfonic acid ( P < 0.001). CONCLUSION It was concluded that taurine improved ruminal microbial crude protein synthesis and increased fiber digestibility through modifying rumen bacterial community. It is necessary to clarify the ruminal hydrolysis of taurine in steers. © 2023 Society of Chemical Industry.
研究领域:
动物营养学
·
微生物学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
Animal
·
2017
·Vol. 12
·期 3
·491-500
·
IF 1.921
Q. Liu,C. Wang,G. Guo,W. J. Huo,Y. L. Zhang,C. X. Pei,S. L. Zhang,H. Wang
Isovalerate supplements could stimulate rumen development by improving morphology and function of rumen mucosa, and then promote the growth of calves. This study was done to evaluate the effects of isovalerate supplements on morphology and functional gene expression of rumen mucosa in dairy calves. In total, 48 Chinese Holstein male calves with 15 days of age and 45.1±0.36 kg of BW were randomly assigned to four groups. The treatments were: control, low-isovalerate, moderate-isovalerate and high-isovalerate with 0, 3, 6 and 9 g isovalerate per calf per day, respectively. Supplementary isovalerate was hand-mixed into milk in pre-weaning calves and into concentrate portion in post-weaning calves. The study consisted of a 15-day-adaptation period and a 60-day-sampling period. Calves were weaned at 60 days of age. Three calves were slaughtered from each of the four treatments at 30, 60 and 90 days of age. The weight of body and stomach were measured, samples of ruminal tissues and blood were analyzed. Total stomach weight, total stomach to BW ratio, rumen wall and keratinized layer thickness, serum growth hormone and IGF-1 for both pre- and post-weaning calves increased linearly with increasing isovalerate supplements. Rumen to total stomach weight ratio, the length and width of rumen papillae, and serum β-hydroxybutyrate increased linearly for post-weaning calves. However, abomasum weight to total stomach weight ratio decreased linearly for both pre- and post-weaning calves. The relative messenger RNA expression for growth hormone receptor, IGF-1 receptor and 3-hydroxy-3-methylglutaryl-CoA synthase 1 in rumen mucosa increased linearly for post-weaning calves. Our results suggested that isovalerate supplements promoted rumen development in a dose-dependent manner. The optimum dose was 6.0 g isovalerate per calf per day.
研究领域:
动物科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Animal Nutrition
·
2024
·
IF 6.1
Jinming Hu,Shuo Zhang,Mengmeng Li,Guangyong Zhao
This study investigated the effects of β-alanine (β-Ala) on ruminal fermentation, nutrient digestibility, nitrogen (N) metabolism, plasma biochemical parameters, and rumen bacterial communities in beef steers. Six steers with initial liveweight of 252.8 ± 5.2 kg and 3 treatments of supplementing with 0, 30, or 60 g β-Ala per day to the basal diet were allocated in a replicated 3 × 3 Latin square design. Each experimental period was 20 d, of which the first 15 d were for adaptation and the subsequent 5 d were for sampling. The results showed that β-Ala linearly increased the ruminal concentration of microbial crude protein (MCP) ( P = 0.002), but it did not affect the ruminal concentrations of ammonia N and total volatile fatty acids ( P > 0.10). β-Ala also linearly increased the dry matter (DM) ( P = 0.009), organic matter (OM) ( P = 0.017) and crude protein (CP) ( P = 0.043) digestibility, tended to decrease the acid detergent fiber digestibility ( P = 0.077), but it did not affect the neutral detergent fiber digestibility ( P = 0.641). β-Ala quadratically increased the relative abundance of ruminal Bacteroidota ( P = 0.021) at the phylum level, and increased Prevotella ( P = 0.028), and Prevotellaceae_UCG-003 ( P = 0.014) and decreased the relative abundance of NK4A214_group ( P = 0.009) at the genus level. Feeding steers with β-Ala linearly increased the urinary N excretion ( P = 0.006) and urea excretion ( P = 0.002) and the N retention ( P = 0.004), but it did not affect the N utilization efficiency ( P = 0.120). β-Ala quadratically increased the plasma concentrations of albumin ( P = 0.048) and linearly increased the plasma concentration of insulin-like growth factor-1 ( P < 0.001) and the total antioxidant capacity ( P = 0.011). In summary, dietary supplementation with β-Ala improved the rumen MCP supply and increased the digestibility of DM, OM, CP and the N retention. Further research is necessary to verify the ruminal degradability of β-Ala and to investigate the mechanism of the impact of absorbed β-Ala on the anti-oxidative ability of the steers.
研究领域:
动物营养学
·
微生物学
·
反刍动物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2016
·Vol. 174
·期 1
·85-92
·
IF 1.798
Zhou Sihui,Zhang Caiying,Xiao Qingyang,Zhuang Yu,Gu Xiaolong,Yang Fan,Xing Chenghong,Hu Guoliang,Cao Huabin
Molybdenum (Mo) is an essential trace element for animals and human beings. However, the negative effects on rumen function and distribution of trace elements in tissues induced by excessive Mo have not been well understood. Therefore, the purpose of present study was to investigate the impact of Mo on rumen microbiota, distribution of trace elements in various organs, and hematological parameters of goats. A total of 36 goats were randomly distributed into three groups with equal number and low-Mo and high-Mo groups were orally administered ammonium molybdate at 15 and 45 mg · Mo · kg −1 · BW respectively, while the control group received corresponding quantitative deionized water. The results showed that the total number of ciliate and protozoa protein concentration decreased significantly ( P < 0.01) on days 25 and 50. Concentrations of ammonia nitrogen and bacterial protein were significantly higher ( P < 0.05) in low-Mo group, while they were lower ( P < 0.05) in high-Mo group than the control group on days 25 and 50. In addition, Mo accumulated in serum and all detected tissues. Copper (Cu) and zinc (Zn) contents significantly decreased ( P < 0.05) in hair and serum on days 25 and 50, while Cu contents increased ( P < 0.05) and the change of Zn contents were not obvious ( P > 0.05) in other tissues on days 25 and 50. Besides, there was no obvious variation in iron (Fe) contents during whole experiment period ( P > 0.05). Furthermore, excessive Mo content had no significant effect on red blood cell (RBC) counts and hemoglobin (HGB) concentration ( P > 0.05) on days 25 and 50, while white blood cell (WBC) counts increased significantly ( P < 0.05) on day 50. These results indicated that excessive Mo content could impact the balance of ruminal microorganisms and interfere with the absorption and distribution of Mo and Cu mainly.
研究领域:
动物营养学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Veterinary Science
·
2023
·
IF 3.2
Zhang Lei,Shen Hong,Zhang Jiyou,Mao Shengyong
Our objective was to investigate the contribution of the rumen microbiome on the individual milk fat percentage (MFP) of Holstein dairy cows under the same nutritional and management conditions. From 92 early lactation dairy cows, the top 10 with the highest MFP (HF; n = 10) and the last 10 with the lowest MFP (LF; n = 10) were selected for the study. As a result, the milk trans-10, cis-12 C18:2 content was significant lower in the HF group than that in the LF group (P < 0.001). The rumen acetate to propionate ratio was significant higher in the HF group than that in the LF group (P = 0.035). According to the results of 16S rRNA gene sequencing, a minor but significant difference existed between the groups (P = 0.040). Three genera of the family Lachnospiraceae and four genera of the order Bacteroidales were identified to be the biomarkers for the LF group and HF group in the LEfSe analysis, respectively. Three microbial modules enriched by the family Lachnospiraceae were positively related to the milk trans-10, cis-12 C18:2 content (rs > 0.60, P < 0.05). According to the results of shotgun metagenome sequencing, three kinds of linoleic acid (LA) isomerase genes were present in the gene pools of the rumen microbiome. Among them, the relative abundance of Bifidobacterium LA isomerase (BBI) was higher in the HF group than that in the LF group (P = 0.007). Three metagenome-assembled genomes (MAGs) with LA isomerase genes were positively correlated to the milk trans-10, cis-12 C18:2 content (rs> 0.40, P < 0.05). Furthermore, all of these three MAGs were found to be able to produce lactate. Taken together, these results indicate that the increased relative abundance of microbial population with the trans-10 biohydrogenation pathway within the rumen microbiome contributes to the decrease of MFP via the increase of rumen trans-10, cis-12 C18:2 production. This study provides a new perspective for the development of measures for improving the milking performance of dairy cows.
研究领域:
动物科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2018
·
IF 1.815
Chao-hua Tang,Jia Liu,Qing-yu Zhao,Jun-min Zhang
A pharmacokinetic comparison of gossypol isomers in cattle was made by investigating their transfer from ingested food to plasma and their degradation by rumen microbes. The gossypol isomers in whole cottonseed, plasma, and rumen fluid were determined by high-performance liquid chromatography (HPLC). The intakes of (+)-and (−)-gossypol by cows on three farms were about 5.6–8.5 and 3.8–5.9 g/(d·herd), respectively. The plasma gossypol concentrations increased as ingestion increased and ranged from 0.31 to 0.48 μg/ml for the (+) form and from 0.39 to 0.59 μg/ml for the (−) form. The (+) form was slightly predominant (58.8%–59.8%) in the gossypol ingested by the cows, whereas the (−) form predominated (54.6%–55.9%) in the plasma. An in vitro study showed that at 6 h, 67.4% and 85.7% of the (−)-gossypol were degraded in 500 and 1000 μg/g treatment groups, respectively, and these increased to 83.6% and 92.5%, respectively, at 12 h. The regularity of the degradation of (+)-gossypol was similar to that of (−)-gossypol. These results showed that (−)-gossypol may be more persistent than (+)-gossypol in plasma, and that the degradation of the gossypol isomers in the rumen is rapid and not enantioselective in cattle.
研究领域:
动物科学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
GENOME BIOLOGY
·
2024
·Vol. 25
·期 1
·1-26
·
IF 10.1
Sun Daming,Bian Gaorui,Zhang Kai,Liu Ning,Yin Yuyang,Hou Yuanlong,Xie Fei,Zhu Weiyun,Mao Shengyong,Liu Junhua
Background The function of diverse ruminal microbes is tightly linked to rumen development and host physiology. The system of ruminal microbes is an excellent model to clarify the fundamental ecological relationships among complex nutrient–microbiome–host interactions. Here, neonatal lambs are introduced to different dietary regimes to investigate the influences of early-life crosstalk between nutrients and microbiome on rumen development. Results We find starchy corn-soybean starter-fed lambs exhibit the thickest ruminal epithelia and fiber-rich alfalfa hay-fed lambs have the thickest rumen muscle. Metabolome and metagenome data reveal that indole-3-carboxaldehyde (3-IAld) and prostaglandin D2 (PGD2) are the top characteristic ruminal metabolites associated with ruminal epithelial and muscular development, which depend on the enhanced ruminal microbial synthesis potential of 3-IAld and PGD2. Moreover, microbial culture experiment first demonstrates that Bifidobacterium pseudolongum is able to convert tryptophan into 3-IAld and Candida albicans is a key producer for PGD2. Transcriptome sequencing of the ruminal epithelia and smooth muscle shows that ruminal epithelial and muscular development is accompanied by Wnt and Ca 2+ signaling pathway activation. Primary cell cultures further confirm that 3-IAld promotes ruminal epithelial cell proliferation depending on AhR-wnt/β-catenin signaling pathway and PGD2 accelerates ruminal smooth muscle cell proliferation via Ca 2+ signaling pathway. Furthermore, we find that 3-IAld and PGD2 infusion promote ruminal epithelial and musculature development in lambs. Conclusions This study demonstrates that early-life ruminal microbiome-derived 3-IAld and PGD2 are effective promoters of rumen development, which enhances our understanding of nutrient–microbiome–host interactions in early life.
研究领域:
动物科学
·
微生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CURRENT MICROBIOLOGY
·
2014
·Vol. 70
·期 1
·67-74
·
IF 1.359
Liu Jing,Pu Yi-Yi,Xie Qian,Wang Jia-Kun,Liu Jian-Xin
Pectin is a non-fiber carbohydrate (NFC) that exists in forages, but it is not clear how pectin exerts its effect on populations of either known microbial species or uncultured ruminal bacteria. PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR analysis were used in the present study to investigate the effects of pectin on microbial communities in an in vitro rumen fermentation system. The fermentations were conducted using forage (corn stover or alfalfa), an NFC source (pectin or corn starch), or their combination as the substrates. Addition of pectin increased acetate ( P < 0.05), whereas inclusion of starch increased butyrate production ( P < 0.05). The pectate lyase activity was higher with alfalfa than with corn straw, or with pectin than with corn starch ( P < 0.05), while the amylase activity was higher in corn starch-included treatments than the others ( P < 0.05). The cluster analysis of the bacterial 16S rRNA gene showed that the DGGE banding patterns differed significantly between the treatments and led to the identification of three groups that were highly associated with the NFC sources. The specific bands associated with pectin-rich treatments were identified to be dominated by members of the Treponema genus. The growth of the Treponema genus was remarkably supported by the inclusion of pectin, highlighting their specific ability to degrade pectin. The results from the present study expand our knowledge of the microbial populations associated with pectin digestion, which may not only facilitate future research on utilization of pectin in feeds, but also improve our understanding of pectin digestion with respect to the rumen micro-ecosystem.
研究领域:
微生物学
·
动物营养学
✓ 已验证
DOI
PubMed
高影响因子
FEMS MICROBIOLOGY ECOLOGY
·
2014
·Vol. 87
·期 3
·576-585
·
IF 3.875
Liu Jing,Wang Jia-Kun,Zhu Wen,Pu Yi-Yi,Guan Le-Luo,Liu Jian-Xin
Treponema saccharophilum is a pectinolytic bacterium isolated from the bovine rumen. The abundance of this bacterium has not been well determined, reflecting the lack of a reliable and accurate detection method. To develop a rapid method for monitoring T. saccharophilum, we performed pyrosequencing of genomic DNA isolated from rumen microbiota to explore the 16S rRNA gene sequences of T. saccharophilum candidates. Species-specific primers were designed based on fifteen sequences of partial 16S rRNA genes generated through pyrosequencing with 97% or higher similarity with T. saccharophilumDSM2985 along with sequence from type strain. The relative abundance of T. saccharophilum was quantified in both in vitro and in vivo rumen systems with varied pectin-containing forages using real-time PCR. There was a clear association of T. saccharophilum with alfalfa hay, which contains more pectin than Chinese wild rye hay or corn stover. The relative abundance of T. saccharophilum was as high as 0.58% in vivo, comparable with the population density of other common rumen bacteria. It is recognized that T. saccharophilum plays an important role in pectin digestion in the rumen.
研究领域:
微生物学
·
分子生物学
·
动物科学
✓ 已验证
DOI
PubMed
高影响因子
Animal Nutrition
·
2025
·
IF 7.5
Manman Fan,Jinming Hu,Cheng Liu,Shuo Zhang,Yufeng Liu,Guangyong Zhao
Taurine is a sulfur-containing nonproteinogenic amino acid. Recent studies have shown that taurine can improve rumen microbial crude protein (MCP) synthesis. This experiment aimed to investigate the action mechanism of taurine on rumen MCP synthesis and nitrogen (N) metabolism in beef steers using sodium sulfate (Na 2 SO 4 ) as a contrast. Six steers (bodyweight 506 ± 17 kg) were assigned to three experimental groups including a basal diet (control), a basal diet supplemented with 45 g taurine/d or 50 g Na 2 SO 4 /d, and were allocated in a replicated 3 × 3 Latin square design. The amounts of sulfur from taurine and Na 2 SO 4 were equal (11.38 g/d). The results showed that, compared with the control group, both taurine and Na 2 SO 4 increased ruminal production of MCP ( P < 0.05) by 37.50% and 29.17%, respectively, and increased the concentration of sulfide (S 2- ) ( P < 0.001). Both taurine and Na 2 SO 4 increased the digestibility of neutral detergent fiber ( P < 0.05). Taurine tended to increase ( P = 0.087) while Na 2 SO 4 decreased plasma urea concentration ( P = 0.049), with the taurine group exhibiting higher plasma urea concentrations than the Na 2 SO 4 group ( P = 0.003). Taurine did not affect urinary urea excretion ( P = 0.246) whereas Na 2 SO 4 decreased urinary urea excretion compared with the control group ( P = 0.002). Compared with the control group, both taurine and Na 2 SO 4 increased urinary allantoin excretion ( P < 0.05), total purine derivatives excretion ( P < 0.05), and the estimated rumen microbial N flow ( P < 0.05). The urinary urea excretion of the taurine group was higher than the Na 2 SO 4 group ( P = 0.019). Compared with the control group, taurine did not affect N excretion, N retention (NR) or N utilization efficiency (NUE) ( P > 0.10), but Na 2 SO 4 decreased urinary N excretion ( P = 0.018) and total N excretion ( P = 0.024), and increased NR ( P = 0.024) and NUE ( P = 0.022). No differences were found in NR and NUE between the taurine and Na 2 SO 4 groups ( P > 0.10). Taurine improved ruminal MCP synthesis by enriching the pathways associated with sulfur and amino acid metabolism while Na 2 SO 4 improved ruminal MCP synthesis by enriching pathways related to nucleotide and purine metabolism. In summary, both taurine and Na 2 SO 4 improved ruminal MCP synthesis by modulating different pathways. Taurine was less effective in decreasing total N excretion than Na 2 SO 4 but no differences in NR and NUE were found between the two groups of beef steers.
研究领域:
动物营养学
·
畜牧学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIVES OF ANIMAL NUTRITION
·
2015
·
IF 1
Shixin Lin,Chen Wei,Guangyong Zhao,Tingting Zhang,Kai Yang
The objectives of the trial were to compare the effects of supplementing rare earth elements (REE) lanthanum (La), cerium (Ce) and praseodymium (Pr) on rumen fermentation, nutrient digestion, methane (CH4) production, nitrogen (N) balance and plasma biochemical parameters in beef cattle. Four Simmental male cattle, aged 12 months, with initial average liveweight of 333 ± 9 kg and fitted with rumen cannulas, were fed with a basal ration composed of concentrate mixture and maize silage. Animals received a basal ration without adding REE (Control) or three treatments, i.e. supplementing LaCl3, CeCl3 or PrCl3 at 204 mg/kg DM to the basal ration, respectively, which were allocated in a 4 × 4 Latin square design. Each experimental period lasted 15 d, consisting of 12 d for pre-treatment and three subsequent days for sampling. Results showed that all tested levels of REE tended to increase neutral detergent fibre digestibility (p = 0.064) and tended to decrease rumen CH4 production (p = 0.056). Supplementing LaCl3 and CeCl3 decreased total N excretion and urinary N excretion, increased N retention (p < 0.05), tended to increase total urinary purine derivatives (PD) (p = 0.053) and microbial N flow (p = 0.095), whereas supplementing PrCl3 did not affect N retention, urinary PD and microbial N flow. No differences were found in the effects of nutrient digestibility, CH4 production and plasma biochemical parameters among LaCl3, CeCl3 and PrCl3. Further trials using graded levels of LaCl3, CeCl3 and PrCl3 in a wide range are needed to obtain more pronounced results for comparing effects of La, Ce and Pr on rumen fermentation and nutrient digestion in beef cattle.
研究领域:
动物营养学
·
畜牧学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiome
·
2023
·Vol. 11
·期 1
·1-15
·
IF 15.5
Fu Yao,Yao Songyang,Wang Tiankun,Lu Yongqiang,Han Huigang,Liu Xuening,Lv Dongying,Ma Xiao,Guan Shengyu,Yao Yujun,Liu Yunjie,Yu Haiying,Li Shengli,Yang Ning,Liu Guoshi
Background Methane (CH 4 ) is a major greenhouse gas, and ruminants are one of the sources of CH 4 which is produced by the rumen microbiota. Modification of the rumen microbiota compositions will impact the CH 4 production. In this study, the effects of melatonin on methane production in cows were investigated both in the in vitro and in vivo studies. Results Melatonin treatment significantly reduced methane production in both studies. The cows treated with melatonin reduced methane emission from their respiration by approximately 50%. The potential mechanisms are multiple. First, melatonin lowers the volatile fatty acids (VFAs) production in rumen and reduces the raw material for CH 4 synthesis. Second, melatonin not only reduces the abundance of Methanobacterium which are responsible for generating methane but also inhibits the populations of protozoa to break the symbiotic relationship between Methanobacterium and protozoa in rumen to further lowers the CH 4 production. The reduced VFA production is not associated with food intake, and it seems also not to jeopardize the nutritional status of the cows. This was reflected by the increased milk lipid and protein contents in melatonin treated compared to the control cows. It is likely that the energy used to synthesize methane is saved to compensate the reduced VFA production. Conclusion This study enlightens the potential mechanisms by which melatonin reduces rumen methane production in dairy cows. Considering the greenhouse effects of methane on global warming, these findings provide valuable information using different approaches to achieve low carbon dairy farming to reduce the methane emission.
Video Abstract
研究领域:
畜牧学
·
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）