MICROCHIMICA ACTA
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2017
·Vol. 184
·期 11
·4269-4277
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IF 4.58
Zheng Yun,Wang Huiqiang,Ma Zhanfang
The authors describe a method for signal amplification of label-free voltammetric immunosensors. A glassy carbon electrode (GCE) was modified with Prussian Blue-platinum nanoparticles (PB-PtNPs) as a redox-active species that gives a strong amperometric signal at 0.18 V (vs. Ag/AgCl). Benefitting from the excellent electrical conductivity and the strong catalytic activity to H 2 O 2 , the modified GCE gives a strongly enhanced signal. The PB-PtNPs were incorporated into a polyaniline (PANI) hydrogel to further enhance the signal. The signal response of the PB-PtNP-PANI/GCE is larger by a factor of 7.6 than that of PB-PtNP/GCE. In order to further improve electrical conductivity and immobilize antibody, gold nanoparticles (AuNPs) were deposited on the surface of the PB-PtNP-PANI hydrogel. The AuNP-PB-PtNP-PANI hydrogel nanocomposite on the GCE was used in an immunosensor for the model analyte carcinoma antigen 125 (CA125), a biomarker for epithelial ovarian cancer, by immobilizing the respective antibody on the modified GCE. A linear response found for the 0.01 to 5000 U mL −1 CA125 concentration range, with a detection limit of 4.4 mU mL −1 (at an S/N ratio of 3). The electrochemical sensitivity is as high as 119.76 μA·(U/mL) −1 ·cm −2 . The detection of CA125 in human serum showed satisfactory accuracy compared to a commercial chemiluminescent microparticle immunoassay (CMIA).
Schematic of a nanocomposites consisting of gold nanoparticles, Prussian Blue, platinum nanoparticles and polyaniline hydrogel as a signal multi-amplification sensing substrate for the ultrasensitive immuno detection of carcinoma antigen 125 (CA125).
研究领域:
生物医学工程
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电化学传感
·
肿瘤标志物检测
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Ruidi Shen,Ji Zhang,Wenxiu Huang,Shaoyong Wu,Gongke Li,Seyin Zou,Liansheng Ling
Cancer antigen 125 (CA - 125) is an important biomarker for the diagnosis of ovarian cancer. In this paper, oligonucleotide 5′-GACAGGCCCGAAGGAATAGATAATACGACTCACTATAGGGAGACAAGAATAAACGCTCAA-3′ (oligo 1) contains an aptamer of CA - 125, and was designed partly complementary to oligonucleotide 5′-CTCTCTCTCCACCTTCTTCTTTGAGCGTTTATTCTTGTCT-3′ (oligo 2). Oligo 1 · oligo 2 was extended with the Klenow fragment (exo − ) polymerase for further polymerase chain reaction (PCR) processes in the presence of two primers: deoxyribose nucleoside triphosphate and Taq polymerase . Single-stranded DNA was produced at two sides of the PCR product by introducing a C 18 spacer into the two primers, which could hybridize with AuNPs-DNA probes, investigated by dynamic light scattering and fluorescence. The addition of CA - 125 can interrupt the hybridization between oligo 1 and oligo 2, causing the average diameter of AuNPs-DNA probes to decrease with the increase of CA-125 within the range of 5 fg mL −1 – 50 ng mL −1 . The linear regression equation of this relationship was D = 430.48–49.60 log 10 C, with a detection limit of 1.1 fg mL −1 . Fluorescein molecules were modified at the end of the forward primer. The fluorescence intensity of the PCR product can be measured simultaneously, with the fluorescence intensity increasing linearly with the logarithm of CA-125 concentration within a linear range from 10 fg mL −1 to 50 ng mL −1 , with a detection limit of 1.5 fg mL −1 .
研究领域:
生物化学
·
分子生物学
·
医学诊断
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Shenguang Ge,Linlin Liang,Feifei Lan,Yan Zhang,Yanhu Wang,Mei Yan,Jinghua Yu
The hybrids, N -aminobutyl- N -ethylisoluminol (ABEI), horseradish peroxidase (HRP) and cancer antigen 125 s antibody (Ab2) immobilized graphene oxide (GO), were prepared, which exhibited excellent chemiluminescence (CL) activity. The zinc oxide nanorods (ZNRs) grew on reduced graphene oxide (RGO) modified paper working electrode (ZNRs-RGO/PWE) via an in situ growth method. The deposition of CdS quantum dots (QDs) on ZNRs/RGO-PWE resulted in an enhanced excitation and photo-to-electric conversion efficiency. The ABEI-GO@HRP/Ab2CL system was employed as an internal excited light source. A photoelectrochemical (PEC) immunosensor based on CdS-sensitized ZNRs anchored RGO as the photoactive matrix and ABEI-GO@HRP/Ab2 as the CL labels was established for sensitive detection of cancer antigen 125 (CA 125). As a model, sandwich CA 125-antibody was taken as molecular reorganization elements on this sensor for the sensitive determination of CA 125 in the linear range from 5.0 × 10 −4 U/mL to 500 U/mL with a detection limit of 2.0 × 10 −4 U/mL. The specificity, reproducibility, and stability of this sensor were also investigated. This proposed method might be attractive for clinical and biomedical applications.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Fangfang Wu,Hongmin Gao,Ren Qiu,Hehua Zhang,Xinshui Ren,Xue Qi,Men Miao,Chuang Rui,Dong Chang,Hongzhi Pan
Accurate and sensitive detection of cancer antigen 125 (CA125) is important in early ovarian cancer diagnosis and monitoring. In the present work, a novel electrochemical ratiometric immunosensor for the determination of CA125 based on three-dimensional reduced graphene oxide/multi-walled carbon nanotube carboxylic acid- thionine (3DrGO/MWCNTs-Thi) and UIO-66-NH 2 /ferrocenecarboxylic acid (UiO-66-Fc) was constructed. 3DrGO/MWCNTs-Thi of immobilized primary antibody (Ab 1 ) and UiO-66-Fc of labeled second antibody (Ab 2 ) were used as capture probes and signaling probes for CA125, respectively. As the concentration of CA125 increases, the oxidation peak currents (I Thi and I Fc ) generated by the two materials were changed in opposite directions, and therefore the ratio of the currents (I Thi /I Fc ) was negatively correlated with the concentration of CA125. Thanks to the amplification of high loading of signaling molecules by the framework nanocomposites and the ratiometric strategy, the constructed electrochemical immunosensor exhibits outstanding accuracy, stability, and sensitivity. Under optimal conditions, the detection range spanned from 0.01 U mL −1 to 80 U mL −1 , with a calculated limit of detection (LOD) of 0.0089 U mL −1 . The immunosensor was successfully applied to real serum sample tests, indicating its potential for clinical detection applications.
研究领域:
生物医学工程
·
分析化学
·
肿瘤学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yingyi Wang,Shanshan Wang,Chunsong Lu,Xiaoming Yang
Malignant tumors posed severe threat to human health, and various efforts have been contributed towards assaying tumor markers, howbeit, achieving to identify the multiple targets by one single analytical mode is in demand. Hereby, three kinds of aptamer-functionalized silver and silver/gold nanoclusters (DNA-AgNCs and DNA-Ag/AuNCs) were originally synthesized, and possessed the specific recognition ability for their corresponding targets. Specifically, the fluorescence of AgNCs or Ag/AuNCs was quenched by introducing graphene oxide (GO), and more competitive interactions between tumor markers and their aptamers occurred with the targets added, thus facilitating their fluorescence recovered. Based on AgNCs or Ag/AuNCs cooperating with GO, this sensing mode was subsequently employed for detecting mucin 1 in a linear range of 1.33–200 ng/mL with a detection limit of 0.18 ng/mL. Meanwhile, this method was also applied to examine carcinoembryonic antigen and cancer antigen 125, and showed the linear range of 6.7 ng/mL–13.3 μg/mL with a detection limit of 3.18 ng/mL for CEA, while CA125 sensing acquired a linear range of 2 ng/mL–6.7 μg/mL accompanied by a detection limit of 1.26 ng/mL. Besides, this strategy provided the potential ways to identify other tumor markers by designing their corresponding nanoclusters with the aptamers.
研究领域:
纳米技术
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生物医学工程
·
分析化学
·
癌症研究
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产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2018
·
IF 2.275
Lide Gu,Wanli Yan,Shu Liu,Wei Ren,Mingsheng Lyu,Shujun Wang
A novel screening method for protein aptamer selection was developed in this study. Aptamers with high affinity and specificity to the surface recombinant antigen of Helicobacter pylori (HP-Ag) and to tumor markers carcinoembryonic antigen (CEA), cancer antigen 125 (CA125) and cancer antigen 19-9(CA19-9) were screened using trypsin enhanced screening method. Briefly, the target proteins above were immobilized onto 96-well polystyrene plates and incubated with a single-stranded DNA (ssDNA) library for aptamer selection. Then, trypsin was introduced to digest the proteins and obtain ssDNA that bound to the target proteins with high specificity. The concentration of ssDNA that shed from protein-ssDNA complexes was detected. After sequencing, the enrichment of target-specific aptamers was monitored and the affinity of each aptamer was analyzed. Urea, which has been reported in other article, was used to compare with trypsin. The results revealed that trypsin was more effective than urea for protein aptamer selection. The protocol used in this study provided a novel method for generating aptamers.
研究领域:
分子生物学
·
生物技术
·
蛋白质工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Li Haiyin,Wang Zhixin,Li Feng,Gai Panpan
Exploring the construction of an interface with bright emission, fabulous stability, and good function to develop high-performance electrochemiluminescence (ECL) biosensors for tumor biomarkers is in high demand but faces a huge challenge. Herein, we report an oriented attachment and in situ self-assembling strategy for one-step fabrication of CdTe QD-encapsulated Hf polymer membrane onto an ITO surface (Hf-CP/CdTe QDs/APS/ITO). Hf-CP/CdTe QDs/APS/ITO is fascinating with excellent stability, high ECL emission, and specific adsorption toward ssDNA against dsDNA and mononucleotides (mNs). These interesting properties make it an ideal interface to rationally develop an immobilization-free ECL biosensor for cancer antigen 125 (CA125), used as a proof-of-concept analyte, based on target-aptamer recognition-promoted exonuclease III (Exo III)-assisted digestion. The recognition of ON by CA125 leads to the formation of CA125@ON, which hybridizes with Fc-ssDNA to switch Exo III-assisted digestion, decreasing the amount of Fc groups anchored onto the electrode’s surface and blocking electron transfer. As compared to the case where CA125 was absent, significant ECL emission recovery is determined and relies on CA125 concentration. Thus, highly sensitive analysis of CA125 against other biomarkers was achieved with a limit of detection down to 2.57 pg/mL. We envision this work will provide a new path to develop ECL biosensors with excellent properties, which shows great potential for early and accurate diagnosis of cancer.
研究领域:
分析化学
·
生物传感
·
纳米技术
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）