MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-16
·
IF 5.3
Li Guiyin,Guo Fei,Liang Jianlu,Wan Bingbing,Liang Jintao,Zhou Zhide
A new sandwich-type electrochemical biosensing platform was developed by gold @polyphthalenediamine nanohybrids (AuNP@PoPD) as the sensing platform and phosphorus doped reduced graphene oxide-hemin-palladium nanoparticles (PrGO-Hemin-PdNP) as the signal amplifier for phosphatidylinositol proteoglycan 3 (GPC3). AuNP@PoPD, co-electrodeposited into the screen printed electrode with high conductivity and stability, is dedicated to assembling the primary GPC3 aptamer (GPC3 Apt ). The second GPC3 Apt immobilized on the high conductivity and large surface area of PrGO-Hemin-PdNP was utilized as an electrochemical signal reporter by hemin oxidation (PrGO-Hemin-PdNP-GPC3 Apt ). In the range 0.001–10.0 ng/mL, the hemin oxidation current signal of the electrochemical aptasensor increased log-linearly with the concentration of GPC3, the lowest detection limit was 0.13 pg/mL, and the sensitivity was 2.073 μA/μM/cm 2 . The aptasensor exhibited good sensing performance in a human serum sample with the relative error of 4.31–8.07%. The sandwich sensor showed good selectivity and stability for detection GPC3 in human serum samples, providing a new efficient and sensitive method for detecting HCC markers. Graphical Abstract
研究领域:
生物医学工程
·
分析化学
·
肿瘤学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Danli Hu,Haiyan Cui,Xinyang Wang,Fang Luo,Bin Qiu,Weicheng Cai,Hui Huang,Jian Wang,Zhenyu Lin
An Au/GaN photoelectrode was prepared by sputtering 30 nm thick Au film on the surface of n-type gallium nitride (GaN). When the electrode contacts with multilayered molybdenum disulfide (MoS2), photogenerated electrons and photogenerated holes transfer to MoS2 because of the band gap matching of MoS2 and GaN. The presence of Au promotes charge transfer and results in a greater recombination of electrons and holes; by this means, a more significant suppression of photocurrent can be detected. This characteristic has been coupled with the high selectivity of an aptamer and applied to develop a novel photoelectrochemical aptasensor for cancer biomarkers (alpha-fetoprotein (AFP) as a model). The aptamer of AFP was modified on the surface of the Au/GaN photoelectrode by Au–S bonds, which can bind to the target protein with high selectivity. Then, the transfer process of the charge carriers of GaN to MoS2 can be blocked by the target protein so that the suppression of photocurrent is reduced. The difference of the photocurrent in the presence and absence of AFP (ΔI) showed a linear relationship with AFP concentration that ranged from 1.0–150 ng/mL (R2 = 0.9995), and the detection limit was 0.3 ng/mL. The standard addition recovery rates ranged from 85.2 to 91.7%. The method possessed good sensitivity and high selectivity for AFP detection. The developed biosensor can be modified to detect other cancer biomarkers by simply replacing the aptamer used.
研究领域:
生物传感技术
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癌症诊断
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材料科学
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Guiyin Li,Bo Wang,Shengnan Li,Xinhao Li,Ruijie Yan,Xiaohong Tan,Jintao Liang,Zhide Zhou
Golgi protein 73 (GP73) is a novel tumor marker in the early diagnosis and prognosis of hepatocellular carcinoma (HCC). Herein, a competitive electrochemical aptasensor for detecting GP73 was constructed using reduced graphene oxide-ferrocene-polyaniline nanocomposite (rGO-Fc-PANi) as the biosensing platform. The rGO-Fc-PANi had larger specific surface area, excellent conductivity and outstanding electroactive performance, which served as nanocarrier for GP73 aptamer (GP73 Apt ) binding and as redox nanoprobe for record electrical signal. Then, a complementary chain (cDNA) was fixed to the electrode by hybridization with GP73 Apt . When GP73 was present, a competitive process happened among cDNA, GP73 Apt and GP73, formed the GP73-GP73 Apt stable chemical structure and made cDNA detach from the sensing electrode, resulting in enhancement of electrical signal. The difference in the corresponding peak current before and after the competition can be used to indicate the quantitative of GP73. Under optimal conditions, the DPV current response showed a good log-linear relationship with GP73 concentrations (0.001 ∼ 100.0 ng/mL) with a detection limit of 0.15 pg/mL (S/N = 3). It was successfully used for GP73 detection in human serum with RSDs ranging from 1.08 % to 6.96 %. Therefore, the aptasensor could provide an innovative technology platform and hold a great potential in clinical application.
研究领域:
生物医学工程
·
肿瘤学
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DOI
PubMed
高影响因子
Alexandria Engineering Journal
·
2025
·
IF 6.8
Xiaohui Wang,Yu Liu,Bude Su,Jinhua Tan,Jianxin Shen,Qi Chen,Yu Gou,Ying Zhang
This study presents a novel electrochemical biosensor for the detection of CA19–9, a crucial pancreatic cancer biomarker, utilizing gold nanoparticles (AuNPs) biosynthesized from mint extract. The green synthesis approach yielded well-dispersed, spherical AuNPs with a characteristic surface plasmon resonance peak at 520 nm. Modification of glassy carbon electrodes with these AuNPs resulted in a significant enhancement of electrochemical properties, including a 50 % increase in peak current and a 66.7 % reduction in charge transfer resistance. The biosensor exhibited a wide linear range from 0.1 to 100 U/mL with excellent linearity (R² = 0.998) and achieved a remarkably low limit of detection of 0.05 U/mL. The sensor demonstrated high specificity against common interfering substances and maintained stability over 100 cycles with less than 5 % decrease in performance. Compared to traditional ELISA methods, this biosensor offers rapid detection, minimal sample preparation, and superior sensitivity. The integration of biosynthesized AuNPs not only enhanced the sensor's performance but also aligned with sustainable practices in nanomaterial synthesis. This innovative approach paves the way for the development of sensitive, eco-friendly, and cost-effective diagnostic tools for early pancreatic cancer detection and monitoring.
研究领域:
生物传感器技术
·
癌症诊断
·
纳米材料合成
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Min Cui,Zhiling Song,Yumin Wu,Bing Guo,Xiaojian Fan,Xiliang Luo
Biocompatible polymers, such as poly(ethylene glycol) (PEG), are of great significance in the development of bio-interfaces and biosensors, as they possess excellent biocompatibility and are easy for modification. A novel highly biocompatible polymer composite was synthesized herein through electrochemical polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a PEG derivative, 4-arm PEG terminated with thiol groups . The electrodeposited conducting polymer composite of PEG doped PEDOT (PEDOT/PEG) exhibited flake-like nanostructure , large surface area and outstanding stability. In order to further immobilize antibodies, gold nanoparticles (AuNPs) were introduced to the PEDOT/PEG composite surface through their unique interaction with the thiol groups . AuNPs modified PEDOT/PEG provided a desirable support for the immobilization of various biomolecules such as antibodies for alpha fetoprotein (AFP), a vital tumor biomarker for liver cancer. The fabricated AFP biosensor demonstrated favorable selectivity, high sensitivity, and ultralow detection limit. Furthermore, owing to the presence of PEG polymers that are highly hydrophilic , such AuNPs/PEDOT/PEG based AFP biosensor also exhibited good anti-fouling ability, and it was capable of assaying target AFP in 10% (V/V) human serum samples, indicating highly feasible potential for clinical diagnosis.
研究领域:
生物医学工程
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材料科学
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肿瘤学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xinqi Luo,Hanxiao Chen,Ge Ling,Wenbo Yue,Bingbing Xie,Kexin Guo,Jing Xu
The self-powered sensing platform uses redox reactions to convert chemical nanogenerators into electrical energy, powering the system for target detection. In this work, a multi-dimensional hollow C@SnO 2 nanocomposite is designed as the electrode substrate and combined with the catalytic hairpin assembly (CHA) amplification strategy. This platform enables highly sensitive detection of the breast cancer marker miRNA-145. C@SnO 2 provides a large specific surface area and excellent electron transport properties, offering abundant enzyme active sites and improving electron transport efficiency. In the presence of miRNA-145, the CHA reaction cycle is triggered, anchoring CHA products to the electrode surface. These signal molecules then participate in the electrochemical reaction, amplifying the signal. Under optimized conditions, the platform demonstrates a linear response range from 1 fM to 10 nM, with a detection limit as low as 0.78 fM. The self-powered sensor enabling sensitive detection of miRNA-145 at low concentrations which is crucial for the early diagnosis of breast cancer. Additionally, this work integrates the self-powered sensing platform with commercial chips, ensuring stable performance and minimal signal fluctuations during long-term continuous monitoring, enabling portable and real-time target monitoring. This design expands the application range of self-powered biosensors and offers new approaches for field detection of other targets.
研究领域:
生物医学工程
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纳米技术
·
癌症诊断
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiang-Cheng Lin,Miaomiao Hu,Minzhe Li,Jian Liu,Qinjie Kou,Rong Huang,Lixian Sun,Changchun Wen
The molecular diagnosis of diseases through the highly sensitive and specific detection of very small amounts of biomarkers in biological samples remains a great challenge, and powerful detection strategies are urgently needed for basic research and clinical applications. Herein, a novel synergistic signal amplification strategy based on Au/Fe 3 O 4 /POPs to integrate magnetic porous composite Fe 3 O 4 /POPs with strong adsorption capabilities for target molecules and gold nanoparticles (AuNPs) with plasmonic properties, is proposed for ultra-high sensitivity and specific Surface-enhanced Raman spectroscopy (SERS) detection of the gastric cancer marker 4-hydroxybenzoic acid (4-HBA). The Au/Fe 3 O 4 /POPs-based SERS assay for gastric cancer-related biomarkers achieved significantly enhanced SERS signals arising from multiple signal amplifications, had good linear calibration from 10 −11 to 10 −5 mol/L, and could detect 4-HBA with a limit of detection (LOD) as low as 1.52 × 10 −12 mol/L. The detection of 4-HBA doped serum exhibited good specificity, homogeneity, and reliability. This material is easy to synthesize and simplifies the sample pretreatment process. This innovative SERS substrate is promising for the rapid detection of disease markers, particularly for early diagnosis and monitoring the effectiveness of gastric cancer treatments.
研究领域:
分析化学
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生物医学
·
纳米材料科学
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lihua Li,Wenzhi Zhang,Huichao Chen,Zhigang Zhao,Meifang Wang,Jiexia Chen
In this work, a method for the visual and electrochemical detection of the breast cancer marker CA15–3 was developed based on the etching reaction of gold/silver core-shell nanoparticles (Au@Ag NPs) by mercury ions (Hg 2+ ). Two kinds of nanocomplex of silicon dioxide nanospheres-antibodies-mercury ions (SiO 2 NSs-Ab-Hg 2+ ) and Au@Ag NPs-antibodies (Au@Ag NPs-Ab) were first prepared in the visual detection. In the presence of antigen, a sandwich structure of immuno-complex was formed through the specific recognition reaction of antigen and antibody. Then, after the addition of pH 4.6 CPBS, redox reaction occurred between Hg 2+ and silver shell. With the increase of the amount of antigen, the silver shell was etched thinner and thinner, and the color changed obviously. Visual detection of CA15–3 could be realized in the range of 30–300 U/mL. In addition, we adopted the DPV signals generated by Hg 2+ etching of silver shell to realize the electrochemical detection of CA15–3. The detection concentration range was 0.1–200 U/mL with a detection limit of 0.05 U/mL.
研究领域:
医学诊断
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纳米生物学
·
分析化学
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DOI
高影响因子
Analytical Methods
·
2021
·Vol. 14
·期 3
·298-305
·
IF 2.896
Dan-Dan Ji,Mei-Xia Wu,Shou-Nian Ding
The combined detection of CA125, CEA and AFP is of great significance in the diagnosis of ovarian cancer. Photonic crystal (PhC) barcodes have apparent advantages in multiplex immunoassays of ovarian cancer markers. In this paper, a novel PhC barcode was assembled from dendritic silica nanoparticles (dSiO2) for multiplex detection of ovarian cancer biomarkers. The interconnected macroporous structure of the dSiO2 PhC beads and the open porous topography of dendritic silica particles could increase the surface area to volume ratio for antibody immobilization. We simultaneously detected multiple ovarian cancer markers in one test tube using the sandwich immunization method by utilizing dSiO2 PhC beads as a barcode and CdTe QDs as a detection signal. The detection limits of the three ovarian cancer markers, AFP, CEA and CA125, were 0.52 ng mL−1, 0.64 ng mL−1 and 0.79 U mL−1, respectively (the signal-to-noise ratio was 3). Compared with the classic silica colloidal crystal bead (SCCB) suspension array, the sensitivity of the dSiO2 PhC bead suspension array was increased. In addition, the results showed that this barcode suspension array had acceptable accuracy and good reproducibility.
研究领域:
生物医学工程
·
肿瘤学
·
纳米技术
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Dandan Fang,Mian Pan,Huan Yi,Hong Dai,Zhensheng Hong,Xiangqin Zheng,Yanyu Lin
Herein, an enhanced lectrochemiluminescence (ECL) immunosensor was designed based on an efficient ECL bioprobe and first realized the quantitative analysis of human epididymis protein 4 (HE4). To obtain the efficient ECL bioprobe, a self-assembled mesocrystalline hybrid (TiO 2 MCs@rGO) was first prepared for loading luminol and 2-(Dibutylamino)ethanol (DBAE), which was explored as a new coreacant of luminol for the first time and triggered more stable and efficient ECL intensity than conventional luminol-H 2 O 2 system. Additionally, the high surface area and outstanding conductivity of TiO 2 MCs@rGO would effectively amplify the ECL intensity. Besides, the synthesized macromolecular complex (PEDOT/PSS/NiCo 2 O 4 NS) integrated the excellent conductivity of PEDOT/PSS and highly porous of NiCo 2 O 4 nanosheets was served as an efficient sensing matrix for further improvement of the analysis sensitivity. The developed ECL immunosensor exhibited excellent performance in the analysis of HE4 with a wide linear range of 1.0 × 10 −5 ˜1000 ng/mL and low detection limit of 3.3 × 10 −6 ng/mL, especially superior stability. The strategy provided promising application for early ovarian cancer monitor and diagnosis, and provoked a new idea for other tumor markers detection.
研究领域:
生物医学工程
·
分析化学
·
肿瘤学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-15
·
IF 5.3
Zhang Shiyu,Chen Xin,Hu Shuai,Cai Ke,Peng Chenxi,Luo Lixia,Gu Yingying,Mei Yong
An electrochemical biosensor was created to identify the liver cancer marker alpha-fetoprotein (AFP) by employing nanocomposite materials. A combination of reduced graphene oxide (rGO) and molybdenum disulfide (MoS 2 ) was selected as the substrate material for the sensor to prepare the PtNPs/MoS 2 @rGO electrochemical immunosensor. Among them, rGO has strong conductivity and MoS 2 provides a large surface area for the anchoring of PtNPs for better attachment to the hybridized nanomaterials. Meanwhile, PtNPs exhibit consistent biocompatibility and excellent electrocatalytic activity. PtNPs also attach to hybrid nanomaterials and bind the antibody via the Pt–S bond, thereby furnishing the antibody with multiple binding sites for enhanced antibody adhesion. The immunosensor achieved ultra-sensitive AFP detection by exploiting the specific antigen–antibody binding. The structure and morphology of the PtNPs/MoS 2 @rGO composites were investigated by transmission electron microscopy (TEM), energy dispersive X-ray (EDS) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy, and the sensor was electrochemically characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimized conditions, using differential pulse voltammetry the biosensor detected AFP in serum within a linear range of 1 ~ 10 5 pg/mL, with a correlation coefficient ( r 2 ) of 0.9989, and a detection limit of 0.12 pg/mL ( S / N = 3). The method offers a new approach for the ultrasensitive detection of serum AFP and is extremely selective, accurate, and precise with a relative standard deviation (RSD) of less than 6%. It has been successfully applied to the analysis of real human blood samples. Graphical
研究领域:
生物医学工程
·
分析化学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·44
·
IF 5.3
Fan Yafeng,Jiao Baoping,Yu Zhongping,Zong Bingbing,Li Haiyan,Wu Xianzhen
A new kind of electrochemical biosensor was fabricated by modifying a glassy carbon electrode (GCE) with Ni-doped ZnO nanorods suspension for the sensitive and selective detection of miRNA-21. The electrode surface was coated with polydopamine (PDA), crosslinked with glutaraldehyde, and functionalized with DNA probes. Bovine serum albumin (BSA) was then used to block non-specific binding sites. Electrochemical analyses were adopted to systematically investigate the progress of the stepwise electrode modification and the hybridization. The electrochemical characterization indicated a gradual decrease in current response and an increase in charge-transfer resistance after each modification step, verifying the successful assembly of the sensing interface. Following optimization, the biosensor could detect concentrations as low as 21.30 fM with a dynamic linear range of 10 − 6 nM to 10 5 nM, good reproducibility, and selectivity to the mismatch and non-complementary sequences. Crucially, recovery experiments in spiked human serum samples gave satisfactory results, confirming the clinical potential of the system. In general, the Ni-doped ZnO nanorod-based biosensor provides sensitive, label-free, and low-cost detection of circulating miRNAs. Its modularity allows for simple conversion for detection of other nucleic acid-based biomarkers, implicating it as a useful diagnostic tool for early cancer diagnosis and point-of-care screening. Graphical abstract
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PubMed
高影响因子
危险品化学品经营许可证（带存储）