BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Chunfang Wang,Yining Wang,Faqiong Zhao,Baizhao Zeng
Organic-inorganic composite photoactive materials can combine the merits of organic and inorganic components and show better performance in comparison with single-component materials. In this work, a novel photoactive material was synthesized through chemical polymerization of 3,4-ethylenedioxythiophene (EDOT) on the surface of hydrothermally prepared Bi 2 WO 6 , followed by modification of self-polymerized dopamine (PDA). The integration of poly(3,4-ethylenedioxythiophene) (PEDOT) and Bi 2 WO 6 effectively facilitated the separation of photogenerated electron-hole pairs, thus demonstrated substantially strong photoelectrochemical signal compared to pure Bi 2 WO 6 . The PDA film, with abundant functional groups, could further sensitize the material and facilitate the immobilization of recognition units. As a proof of application, the composite was combined with carcinoembryonic antibody to construct an immunosensor for carcinoembryonic antigen sensing. Resultantly, although the components and construction procedure were simple, the prepared sensor demonstrated a broad linear detection range of 0.001–20 ng/mL and a relative low detection limit of 0.50 pg/mL. It also showed high selectivity and stability. Its practical feasibility was confirmed by detecting real samples and yielding satisfactory results.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Lei Shang,Xiao Wang,Wei Zhang,Li-Ping Jia,Rong-Na Ma,Wen-Li Jia,Huai-Sheng Wang
Ratiometric electrochemiluminescence (ECL) assays have attracted widespread attentions in biosensing owing to their precise measurements by eliminating the environmental interferences. However, they mostly needed two eligible luminophors, increasing the complexity of the systems and limiting their practical applications. Herein, using luminol as single luminophor, a dual-potential ratiometric ECL strategy was proposed to detect carcinoembryonic antigen (CEA). The luminol exhibited cathodic and anodic emissions on graphene-ionic liquid-platinum (GR-IL-Pt) composites and Ti 3 C 2 MXenes-Au NPs hybrids, respectively. Then, a sandwich ECL sensor was fabricated using GR-IL-Pt composites as matrix to immobilize the primary antibodies of CEA and Ti 3 C 2 MXenes-Au NPs hybrids as platform to load the secondary antibodies. With the presence of CEA, the ratio of anodic ECL to cathodic ECL (ECL anodic /ECL cathodic ) increased obviously, realizing sensitive ratiometric detection of CEA. In addition, the ECL anodic /ECL cathodic was independent with the concentrations of H 2 O 2 , greatly improving the test reliability. The developed ECL sensor exhibited a sensitive detection toward CEA, performing a wide linearity in the range of 0.1 pg mL −1 - 10 ng mL −1 with a low detection limit of 34.58 fg mL -1 (S/N = 3). Furthermore, this strategy exhibited a good practicality to detect CEA in human serums, providing a promising strategy in ECL bioanalysis.
研究领域:
生物传感
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Yange Luan,Qijie Ma,Yihong Hu,Xinyi Hu,Guanyu Chen,Jiaru Zhang,Zhong Li,Dongdong Xie,Guanghui Ren,Yunyi Yang,Guifu Ding,Kate Fox,Bao Yue Zhang,Jian Zhen Ou
2D nanomaterials have shown significant advances in next-generation biosensor development. However, current 2D-enabled electronic biosensors rarely achieve a detection limit down to pico-gram level, possibly due to their intrinsic low carrier mobilities, biocompatibility or instability. TiO 2 , a biocompatible material, has been widely adopted in bio-applications. Recently, 2D TiO 2 with a unique planar hexagonal phase exhibits a reduced bandgap of 2 eV and high p-type mobility. A small perturbation could stimulate an observable electronic swing, enabling a high-performance biosensor in a label-free manner. Here, 2D h-TiO 2 is fabricated into a liquid-gated field-effect transistor, while a wide spectrum, nonspecific biomarker carcinoembryonic antigen (CEA) is selected as the target molecule. The h-TiO 2 surface is first functionalized with 3-aminopropyltriethoxysilane and subsequently decorated with anti-CEA, creating an affinity probe toward CEA molecules. The real-time response to different concentrations of CEA is tested from 1 pg mL −1 to 10 ng mL −1 under a low working voltage of 0.05 V, and an ultralow detection limit of 0.22 pg mL −1 is achieved. Meanwhile, the selectivity of the devices is demonstrated using cytokeratin-19-fragment and neuron-specific enolase. The results highlight the potential of layered hexagonal metal oxides for high-performance biosensors, enabling future low-power portable devices for disease diagnosis.
研究领域:
纳米材料
·
生物传感器
·
电子器件
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生物医学诊断
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Shuai Ma,Minghao Zhang,Fuxiang Song,Wenhao Yang,Liantao Xin,Caifeng Ding
The electrochemical antifouling sensing interface can effectively resist the adsorption of non-specific biomolecules , which provides a powerful means for the sensitive determination of various biomarkers in complex media. Therefore, the development of simple and efficient antifouling coatings is particularly important. Herein, we utilized tris (2-carboxyethyl) phosphine to rapidly reduce disulfide bonds in bovine serum albumin (BSA) to generate amyloid aggregation (PTB), thus forming a macroscopic two-dimensional film through one-step self-assembly. Compared to other antifouling interfaces, PTB has the advantages of simple preparation means, high stability and reproducibility, as well as good antifouling properties. At the same time, a layer of polyaniline (PANI) was electrodeposited on the modified electrode to improve the electron transmission rate, which improves the detection efficiency of the sensor. Furthermore, g-C 3 N 4 was introduced as an internal standard substance to eliminate instrument errors. According to the ratio of electrochemiluminescence (ECL) signals of [Ru(bpy) 3 ] 2+ and g-C 3 N 4 , a ratiometric sensing strategy was designed to improve detection accuracy and sensitivity. Based on this, the proposed biosensor can achieve sensitive detection of CEA with a detection limit as low as 0.43 fg/mL ( S/N = 3), which has important significance for early clinical diagnosis.
研究领域:
生物传感器
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医学诊断
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 12
·1-11
·
IF 5.833
Wang Xiao,Liao Xiaochen,Zhang Bingjian,Zhang Mengmeng,Mei Lisha,Wang Fangwai,Chen Siyu,Qiao Xiuwen,Hong Chenglin
Abstract Carcinoembryonic antigen levels in the human body reflect the conditions associated with a variety of tumors and can be used for the identification, development, monitoring, and prognosis of lung cancer, colorectal cancer, and breast cancer. In this study, an amperometric immunosensor with CuCo/carbon nanocubes (CuCo/CNC) as the signal label is constructed. The bimetal-doped carbon skeleton structure has a high specific surface area and exhibits good electrocatalytic activity. In addition, Au/g-C 3 N 4 nanosheets (Au/g-C 3 N 4 NSs) are used to modify the substrate platform, facilitating the loading of more capture antibodies. The reaction mechanism was explored through electrochemical methods, X-ray powder diffraction, X-ray photoelectron spectroscopy, and other methods. Kinetic studies have shown that CuCo/CNC have good peroxidase-like activity. In addition, the electrocatalytic reduction ability of CuCo/CNC on hydrogen peroxide can be monitored using amperometric i – t curve (− 0.2 V, vs. SCE), and the response current value is positively correlated with the CEA antigen concentration. The prepared electrochemical immunosensor has good selectivity, precision, and stability. The dynamic range of the sensor was 0.0001–80 ng/mL, and the detection limit was 0.031 pg/mL. In addition, the recovery and relative standard deviation in real serum samples were 97.7–103 % and 3.25–4.13 %, respectively. The results show that the sensor has good analytical capabilities and can provide a new method for the clinical monitoring of CEA. Graphic abstract
研究领域:
生物医学工程
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肿瘤学
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分析化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Qianshun Chen,Qiuyan He,Yiting Wang,Chen Huang,Yuyu Lin,Jieyang Wang,Wei Shen,Bin Qiu,Xunyu Xu
Carcinoembryonic antigen (CEA) is the most widely adopted biomarker for the early determination of various cancers, thus it is very important to design a simple and highly effective sensing method to detect CEA. Herein, by synthesizing Fe-MOF nanohybrid loaded with platinum nanoparticles (Pt NPs/Fe-MOF) nanozyme and selecting CEA aptamer (Apt) as the specific recognition element, a simple nanozyme-based voltammetric sensor was designed to detect CEA based on Apt-controlled the catalytic activity of Pt NPs/Fe-MOF nanozyme. The as-synthesized Pt NPs/Fe-MOF nanozyme could catalyze effectively 1,2-diaminobenzene oxidation to produce electroactive 2,3-diaminophenazinc (DAP) which can exhibit a strong reduction current. Nonetheless, when Pt NPs/Fe-MOF is covered fully by Apt of CEA, the peroxidase activity of the as-synthesized Pt NPs/Fe-MOF nanozyme is remarkably restricted and the peak current of DAP would disappear nearly. Interestingly, this catalytic activity could be recovered effectively in the presence of CEA owing to the specific identification of Apt toward CEA. Through optimizing several experimental factors, the as-designed nanozyme voltammetric sensor offers superior detective performance for CEA. In addition, the as-designed sensor possesses superior specificity and selectivity, reproducibility and stability as well as practical applications, showing great potentials for achieving simple, sensitive and highly effective determination of CEA.
研究领域:
生物化学
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医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2012
·
IF 3.898
Tingting Hao,Zhiyong Guo,Shuping Du,Lili Shi
An ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA) in serum and saliva was developed. Ru(bpy) 3 2+ -graphene-Nafion composite was first coated on the glassy carbon electrode surface in this ECL immunosensor. The sandwich-type immunoreactions between the first antibody and the second antibody bridged the donors Ru(bpy) 3 2+ and acceptors QDs , which led to the occurrence of ECL quenching of Ru(bpy) 3 2+ by QDs . Based on the efficient ECL quenching, ultrasensitive CEA detection was realized. Under optimal conditions, the ECL signal depended linearly on the logarithm of the CEA concentration within a range from 0.005 to 0.5 pg/mL, and the detection limit of CEA was 0.002 pg/mL. Moreover, the proposed ECL immunosensor provides a stable, specific, and highly sensitive immunoassay protocol for CEA detection at very low levels, which might hold a promise for clinical application.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Yongze Xie,Min Zhang,Qianyun Bin,Shilei Xie,Lu'an Guo,Faliang Cheng,Weizhong Lv
The fast and accurate detection of Carcinoembryonic Antigen (CEA) plays an important role in clinical cancer treatment and therapy. An ultrasensitive photoelectrochemical (PEC) immunosensor for the detection of CEA was constructed using CdSe@BiVO 4 co-sensitized TiO 2 nanorods as photoactive materials. TiO 2 nanorods were assembled on the FTO modified electrode to immobilize capture antibodies. With a sandwich immunoassay format, CEA and signal antibodies labelled CdSe@BiVO 4 were introduced in sequence via specific immunoreaction, and the ultrahigh sensitivity of this immunoassay results from the following three aspects. Firstly, the co-sensitization of BiVO 4 and CdSe extends the absorption range of TiO 2 from ultraviolet region to visible light region, which can adequately utilize the light energy; Secondly, the effective matching of energy levels among TiO 2 , CdSe and BiVO 4 accelerates the separation and transfer of photogenerated electron-hole pairs and significantly improves the PEC performance; Finally, the introduced Au evidently expedites the interfacial electron transfer from TiO 2 to FTO electrode, further resulting in noticeably increased photocurrent . Based on multiple signal amplification strategy, a largely linear detection range from 0.01 ng mL −1 to 50 ng mL −1 with a low detection limit (0.5 pg mL −1 ) were obtained. In addition, the prepared immunosensor with attractive properties provides a promising platform for PEC detection.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 1
·103
·
IF 5.4
Xu Li,Jiali Huang,Jiayu Ding,Mingzhen Xiu,Kang Huang,Kang Cui,Jing Zhang,Shiji Hao,Yan Zhang,Jinghua Yu,Yizhong Huang
A dual-mode lab-on-paper device based on BiVO4/FeOOH nanocomposites as an efficient generating photoelectrochemical (PEC)/colorimetric signal reporter has been successfully constructed by integration of the lab-on-paper sensing platform and PEC/colorimetric detection technologies for sensitive detection of carcinoembryonic antigen (CEA). Concretely, the BiVO4/FeOOH nanocomposites were in situ synthesized onto the paper-working electrode (PWE) through hydrothermal synthesis of the BiVO4layer on cellulose fibers (paper-based BiVO4) which were initially modified by Au nanoparticles for improving the conductivity of three dimensional PWE, and then the photo-electrodeposition of FeOOH onto the paper-based BiVO4to construct the paper-based BiVO4/FeOOH for the portable dual-mode lab-on-paper device. The obtained nanocomposites with an FeOOH needle-like structure deposited on the BiVO4layer exhibits enhanced PEC response activity due to its effective separation of the electron–hole pair which could further accelerate the PEC conversion efficiency during the sensing process. With the introduction of CEA targets onto the surface of nanocomposite-modified PWE assisted by the interaction with the CEA antibody from a specific recognition property, a signal-off PEC signal state with a remarkable photocurrent response decreasing trend can be achieved, realizing the quantitative detection of CEA with the PEC signal readout mode. By means of a smart origami paper folding, the colorimetric signal readout is achieved by catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue oxidized TMB in the presence of H2O2due to the satisfied enzyme-like catalytic activity of the needle-like structure, FeOOH, thereby achieving the dual-mode signal readout system for the proposed lab-on-paper device. Under the optimal conditions, the PEC and colorimetric signals measurement were effectively carried out, and the corresponding linear ranges were 0.001–200 ng·mL−1and 0.5–100 ng·mL−1separately, with the limit of detection of 0.0008 and 0.013 ng·mL−1for each dual-mode. The prepared lab-on-paper device also presented a successful application in serum samples for the detection of CEA, providing a potential pathway for the sensitive detection of target biomarkers in clinical application.Keywords:PEC;colorimetric;lab-on-paper device;BiVO4/FeOOH;CEA
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Wei Wen,Jing-Yi Huang,Ting Bao,Jun Zhou,Hong-Xing Xia,Xiu-Hua Zhang,Sheng-Fu Wang,Yuan-Di Zhao
We report a triplex signal amplification strategy for sensitive biosensing of cancer biomarker by taking advantage of hairpin-shaped oligonucleotide-functionalized gold nanorods (HO-GNRs), graphene and the avidin-biotin reation. The strategy expands electrochemical detection of carcinoembryonic antigen (CEA) by using an aptamer as biosensor's recognition element and HO-GNRs as signal enhancer. To construct this biosensor, the GNR was used as a carrier of horseradish peroxidase (HRP) and HO aptamer with a biotin at the 3′-end and a thiol at the 5′-end, which amplified the electrochemical response because of a large molar ratio of HRP to HO. In the presence of target CEA, the binding reactions of CEA with the loop portions of the HOs caused HOs’ loop-stem structure opened and exposed the biotins, and then HRP-GNRs-HO conjugates were captured on graphene and streptavidin modified electrodes via the reaction between the exposed biotins and preimmobilized streptavidins. The accumulation of HRP effectively catalyzed the hydrogen peroxide-mediated oxidation of o -phenylenediamine to generate an electrochemical reduction current for CEA detection. Under optimal conditions, the electrochemical biosensor exhibited a wide dynamic range of 5 pg mL −1 and 50 ng mL −1 toward CEA standards with a low detection limit of 1.5 pg mL −1 (signal-to-noise ratio of 3). The proposed biosensor accurately detected CEA concentration in 8 human serum samples from patients with lung diseases, showing excellent correlations with standard chemiluminescence immunoassay . Furthermore, these results of target DNA detection made it abundantly clear that the proposed strategy can also be extended for detection of other relative biomarkers using different functional DNA structures , which shows great prospects in single-nucleotide polymorphisms analysis, biomedical sensing and application for accurate clinical diseases diagnostic.
研究领域:
生物传感器技术
·
医学诊断
·
纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Haoran Tang,Hao Wang,Cheng Yang,Dandan Zhao,Yuanyuan Qian,Yongxin Li
Nanopores have become one of the most important tools for single-molecule sensing, but the challenge for selective detection of specific biomolecules still exists. In this contribution, we develop a new technique for sensing carcinoembryonic antigen (CEA), one of the important cancer biomarkers, using solid-state nanopores as a tool. The method is based on the specific affinity between aptamer (Apt) modified magnetic Fe3O4–Au nanoparticles (MNPs) and CEA, and the formed CEA–Apt–MNPs and remaining Apt–MNPs can transport the nanopores by applying a positive potential after magnetic separation. Due to the obvious particle size difference between CEA–Apt–MNPs and Apt-MPs, their corresponding blockage signals could be distinguished completely by the degree of the current decline. Moreover, the frequency of the blockage signals for CEA–Apt–MNPs is proportional to the concentration of CEA within certain limits, indicating that our designed nanopore sensing strategy can quantitatively detect CEA in complex samples. This work demonstrates that our designed nanopore-based strategy can be used for CEA sensing with good selectivity and sensitivity and also can be used to analyze other protein biomarkers for early diagnosis and monitoring of cancer, though the detection limit (0.6 ng/mL) is not relatively low. In future works, we plan to improve our detection limit by the improvement of the nanopipette preparation technology and detection method.
研究领域:
生物物理学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Nian-Lu Li,Li-Ping Jia,Rong-Na Ma,Wen-Li Jia,Yi-Yang Lu,Sha-Shan Shi,Huai-Sheng Wang
In this study, a novel sandwiched electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA) was developed. The nanocomposite of polydopamine and Ag nanoparticles (PDA-AgNPs) was prepared by the redox reaction between Ag + and dopamine. This nanocomposite not only provided an effective matrix for the immobilization of primary antibody (Ab 1 ) but also enhanced the conductivity of the electrode. Carbon quantum dots (CQDs) were immobilized on the poly(ethylenimine) functionalized graphene oxide (PEI-GO) through amido-bond. Then Au nanoparticles were decorated on the CQDs modified PEI-GO matrix, and the resulted complex AuNPs/CQDs-PEI-GO was introduced to link secondary antibody (Ab 2 ). The CQDs can be connected to the electrode surface through the combination of CEA with Ab 1 and Ab 2 , and then the amplified electrochemiluminescence signal of CQDs was obtained with the synergistic effect of AgNPs , polydopamine, AuNPs and PEI-GO. Under the optimal conditions, the ECL intensity was proportional to the logarithm value of CEA concentration in the linear range from 5 pg mL −1 to 500 ng mL −1 with a detection limit of 1.67 pg mL −1 for CEA detection. The immunosensor was applied for the CEA detection in real samples with satisfactory results. The proposed ECL immunosensor showed good performance with high sensitivity, specificity, reproducibility, stability and will be potential in clinical detection.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）